Background/Objectives: The research concerns a theoretical and methodological rationale for the use of digital educational resources as a means for differentiation of students’ training in computer science. In this context, this article aims to identify and disclose the use of specialized software, allows performing the learning process analysis and, depending on the result, giving students assignments corresponding to the level of their training, and promptly providing the teacher or the students the necessary digital educational materials, not diverting them from their main job. Methods: The basic provisions of the theory of knowledge, logic and science, methodology of the system approach and the appropriate psycho-educational treatment of ‘activity’ concept constitute the methodological basis of the research. Findings: The structure of the digital practice and training complex has been elaborated; didactic possibilities of the above complex have been identified; a methodology for using this complex in the conditions of differentiated instruction has been developed, the factors have been defined that are relevant in the context of differentiated learning organization using digital practice and training complex, which is a significant contribution and a practical result of this research, they can be used in teaching practice. Improvements: Materials of this article are of practical value; the results of the study were discussed at the meetings of methodological seminar of the National Center of Information Technologies, were presented at international scientific conferences and published in the republican scientific and educational publications.