Federal State Budgetary Scientific Institution Research Articles

Study of culture filtrates of anthracnose pathogen strains for use in in vitro flax breeding for pathogen resistance

The studies were carried out on the basis of the laboratory of breeding technologies of the Federal State Budgetary Scientific Institution “Federal Scientific Center for Bast Crops” (Tver region) in 2018–2020. The aim of the research is to create in vitro new flax genotypes, resistant to anthracnose, one of the most harmful fungal diseases. As a result of the research, the composition of the culture filtrate of the anthracnose causative agent was clarified. It was revealed that the toxicity of the culture filtrates did not depend on the virulence of the strains used in the present studies — the cultural filtrates of strains 784 (highly virulent) and 780 (medium virulent) were more toxic (decay and death of primary roots on day 5 was observed in 67–88% grown seeds), strains 793 (strongly virulent) and 788 (weakly virulent) are less toxic (on the 5th day, decay and death of primary roots was noted in 9–15% of germinated seeds). It was found that morphogenic foci were formed more actively in genotypes, the morphogenic callus of which was transferred to a medium with a higher concentration of the culture filtrate; it was shown that in the second passage, when transferring morphogenic callus from a selective medium containing 40 ml/l of culture filtrate to a selective medium, containing also 40 ml/l of culture filtrate, as well as when transferring morphogenic callus from a selective medium containing 40 ml/l of culture filtrate, on a selective medium, containing 44 ml/l of culture filtrate, on the 14th day the number of formed morphogenic callus and green buds is significantly greater than when transferred from a selective medium, containing 40 ml/l of culture filtrate to a selective medium, containing 36 ml/l of culture filtrate. Viable regenerant plants were obtained and genotypes were isolated, which for three generations retained resistance to anthracnose at a level of 50–60%: NO-78 x Lenok, HJI-103-2 x Lenok, NL-40-1 x Lenok, NE -38 x Rosinka, NE-36 x Lenok, NE-17 x Lenok, HE-16-2 x Rosinka.

Responsiveness of a new variety of spring barley Notable to the seeding rate in the Ryazan region

Relevance. At present, with the change in economic conditions and the possibilities of agricultural production, it is necessary to clarify in some cases and revise complex agro-technological methods for growing barley that meet the requirements of intensive technology. An increase in grain production is impossible without the development of modern, costly technologies for growing grain crops. Methods. In the conditions of the Institute of seed production and agricultural technologies – a branch of the Federal state budgetary scientific institution "Federal scientific Agroengineering center VIM" (Ryazan region) in 2018-2020, field studies were conducted to establish the optimal seeding rate for a new variety of spring barley Notable, which provides the highest and stable grain yield over the years. We studied seeding rates with an interval of 0,5 million germinating grains per hectare (3,0; 3,5; 4,0; 4,5; 5,0 and 5,5 million). Results . It was found that the seeding rates have a significant impact on the size and quality of the barley grain yield. It was found that a decrease in the rate of seeding of seeds of the Noble variety leads to an increase in the percentage of germination and preservation of plants before harvesting. Thus, the correlation analysis revealed a close relationship between the yield and the preservation of plants for harvesting (r=+0,86). Based on the observation of the phenological phases of this crop, it was found that the date of their occurrence and the duration of interphase periods did not depend on the seeding rates, but had a close relationship with weather conditions (r= + 0,71). Option with a seeding rate of 3,0 million crops. grains/ha formed the maximum tillering coefficient, had the highest productivity of one plant and the number of grains per ear compared to other variants of the experiment – 3,2; 3,6 and 25,3, respectively. In connection with the thickened sowing on the variant of 5,5 million vsh. grain / ha, there was an increase in the development of most leaf diseases by 1,0-1,5 points compared to the variant of 3,0 million vsx. grains/ha and by 0,5-1,0 points compared to the other variants of the experiment. The highest yield over the years of research was achieved with a seeding rate of 3,0 to 4,5 million crops. grain/ha – 6,4-6,5 t/ha.

Neuromuscular electrical stimulation in early rehabilitation of patients with postoperative complications after cardiovascular surgery

Abstract Funding Acknowledgements Type of funding sources: Public hospital(s). Main funding source(s): Federal State Budgetary Scientific Institution Research Institute for Complex Issues of Cardiovascular Diseases Introduction The purpose was to evaluate the effectiveness of NMES in patients with complications after cardiac surgery. Methods This study was 37 patients who had significant postoperative complications after cardiovascular surgery. Participants were randomly - NMES group, n = 18; control, n = 19. It was not possible to blind the investigator. Analyzed basic clinical data. The dynamometry of the muscles upper and lower extremities was carried out, as well as a 6-minute walk test (6MWT). Also, the thickness of the quadriceps was measured using ultrasound.The patients underwent NMES on the quadriceps femoris muscle, daily from the third postoperative day, until discharge. The duration session was 90 minutes. Outcomes No differences were found in the baseline characteristics of the groups, including the results of laboratory and instrumental studies. Groups were comparable in the surgery and perioperative parameteres. The initial strength indicators also had no significant differences in the groups. At discharge knee extensors strength (KES) was significantly higher in the NMES group. The knee flexor strength (KFS) and handgrip strength (HF) increased the same in both groups. The quadriceps crosssectional area (CSA) muscle increased more in the NEMS group than in the control to the time of discharge. Average KES increased to a greater extent in the NMES group. At the same time, average and maximum KFS increased equally in both groups. A 6MWT before discharge did not show a difference between groups (P=.166). The NMES course did not affect the duration of hospitalisation (P=.429). Discussion This pilot study show beneficial effects of NMES on muscle strength in patients with complications after cardiovascular surgery. Physical tests initially and in dynamics NEMS Group (n = 18) Control group (n = 19) Baseline Discharge Baseline Discharge P-level Right knee extensors strength (kg) 20,3 [17,9; 26,1] 28,05 [23,8; 36,2] * 20,1 [18,6; 25,4] 22,3 [20,1; 27,1] * 0,004 Left knee extensors strength (kg) 17,75 [15,5; 27,0] 27,45 [22,3; 33,1] * 20,8 [17,5; 24,2] 22,5 [20,1; 25,9] * 0,017 Right knee flexors strength (kg) 14,85 [11,7; 19,5] 17,5 [14,1; 23,4] * 16,9 [13,1; 23,8] 19,2 [12,5; 26,4] * 0,971 Left knee flexors strength (kg) 14,7 [12,6; 19,6] 19,75 [15,9; 24,2] * 16,2, [10,4; 25,1] 18,8 [13,1; 27,7] * 0,889 6-MWT (m) 148,5 [108,5; 174,0] 288,0 [242,0; 319,0] * 169,0 [115,0; 217,0] 315,0 [277,0; 400,0] * 0,166 Right handgrip strength (kg) 24,5 [15,0; 33,0] 25,5 [19,0; 36,0] * 27,0 [18,0; 32,0] 30,0 [20,0; 35,0] * 0,795 Left handgrip strength (kg) 17,0 [12,0; 27,0] 21,0 [15,0; 31,0] * 19,0 [14,0; 29,0] 23,0 [16,0; 30,0] * 0,541 * - p-level from baseline data < 0,05 ** - p-level from baseline data ≥ 0,05

Psychological status of patients after coronary artery bypass grafting: Data from a 5-year-follow-up study

Abstract Funding Acknowledgements Type of funding sources: Public Institution(s). Main funding source(s): Federal State Budgetary Scientific Institution “Research Institute for Complex Issues of Cardiovascular Diseases” Negative psychological status is acknowledged as one of risk of development of cardiovascular pathology. However, predictive value of type D personality (psychosocial distress) in different cultures and the countries are not fully understood. Purpose: Study aim was to investigate the association of type D personality and quality of life (QoL) with the development of cardiovascular complication in patients in five years after coronary artery bypass grafting (CABG). Materials and Methods: Evaluation of psychological status and combined risk of nonfatal (strokes, nonfatal myocardial infarctions, repeat procedures of revascularization and hospitalization due to relapse or progression of angina pectoris) and fatal (general and cardiovascular mortality) cases before and in five years after CABG. Patients with chronic ischemic heart disease (n = 602, 112 (18.5%) females and 490 (81.5%) males, mean age = 57.7 ± 7.3 years.) who had had CABG were included in the study. Who were formed in two groups: patients with the presence of type D (n= 134) and patients without type D (n = 468). The study of the psychological status was carried out using questionnaire DS-14. Analysis of long-term results were assessed using the Kaplan–Meier method. Data was collected by a SF-36 questionnaire, comprising of 36 questions divided in 8 domains. The score was designated as 0 to 100; with the higher score being indicative of a better QoL. Results: Frequency of fatal cases in both groups amounted 8.3% and 8.1% (p = 0.145), in turn nonfatal end-points in group with type D amounted 31.8%, and 15.9% in group without type D (p = 0.044) in during the 5 years. Presence of type D personality at initial examination increased 3.21 times combined risk of nonfatal and fatal cases (OR 3.21, 95% CI 2.02-6.14, p = 0.002). In long term period there were no differences in survival between groups type D and without type D (95.5% vs. 96.8%), There were differences in groups type D and without type D in nonfatal events (strokes, nonfatal myocardial infarctions, secondary endpoint: repeat procedures of revascularization and hospitalization due to relapse or progression of angina pectoris) (respectively, 97.1% vs 88.0%, (p = 0.341). Findings showed that 75% of subjects reported well QoL, while the mean score regarding QoL in patients with type D personality, were higher than in patients without type D in all health related dimensions. Based on t-test difference, the of QoL in in patients with type D and in patients without type D for physical health (p < 0.015), mental limitation (p < 0.043), somatic pain (p < 0.022) and mental health (p < 0.041) was observed. Conclusion: Type D patients had a greater risk for nonfatal cardiac events and lower QoL indicators, compared with non Type D patients. Accumulated proofs indicate that in detection of patients at risk of development of stress induced cardiac complications after CABG it is reasonable to use approach which involves consideration of personality type.

Cardio-ankle vascular index in persons with prediabetes and diabetes mellitus in a population sample of Western Siberia of the Russian Federation

Abstract Funding Acknowledgements Type of funding sources: Public Institution(s). Main funding source(s): Federal State Budgetary Scientific Institution “Research Institute for Complex Issues of Cardiovascular Diseases” The aim of this study was to evaluate the level of cardio-ankle vascular index (CAVI) in the presence of pre-diabetes and diabetes mellitus in the population sample of the Russian Federation, as well as to investigate the factors associated with pathological CAVI in these subjects. Methods 1628 (age 25-64 years) were recruited in a observational cross-sectional study ESSE-RF in the period from 2012 to 2013. The standard protocol of the ESSE-RF has been extended with additional study of CAVI. The final sample included 1617 people, they are divided into 3 groups: group 1 - patients with type 2 diabetes mellitus (n = 272), group 2 - patients with prediabetes - IFG, IGT or their combination (n = 44), and group 3 - persons without CMD (n = 1301). Results The median CAVI was higher in diabetes and pre-diabetes groups (p = 0.009 and p <0.001, respectively, compared with group without CMD). Pathological CAVI (≥9.0) was detected in 16.8% of diabetes patients, in 15.9% of those with prediabetes, and in 9.0% of those without CMD (p <0.001 when comparing diabetes group and group without CMD). The groups with diabetes and prediabetes also did not differ in factors affecting the level of arterial wall stiffness (gender, blood pressure, age, dyslipidemia, uric acid level). According to the result of the correlation analysis in the general sample, CAVI positively correlated with age (p <0.001), male sex (p <0.001), type 2 diabetes (p <0.001), prediabetes (p = 0.002), waist circumference (p <0.001), visceral obesity (p <0.001), total cholesterol (p <0.001), triglycerides (p <0.001), LDL cholesterol (p <0.001), and with the inverse correlation - with glomerular filtration rate (GFR, p <0.001). In multivariate analysis, age and the presence of arterial hypertension were independently associated with pathological CAVI in the groups of patients with CMD, and in the group without CMD, in addition to these factors - a decrease in GFR. Conclusions In the population sample of the Russian Federation, the CAVI index values in the prediabetes and diabetes patients were higher than in the normoglycemia persons. The groups with diabetes and prediabetes also did not differ in factors affecting the level of arterial wall stiffness. Since the identified disorders of arterial stiffness in prediabetes are similar to those in diabetes, their identification is important to prevent further cardiovascular complications.

Dynamics of blood pressure indicators in patients with diabetes mellitus after planned percutaneous coronary intervention

Arterial hypertension (AH) is a common modifiable cardiovascular risk factor. Despite the wide choice of antihypertensive drugs, according to epidemiological studies, just over 20% of patients demonstrate effective control of hypertension. Failure to achieve the target values of blood pressure (BP) determines a high probability of developing cardiovascular events, which is also true for patients undergoing percutaneous coronary interventions (PCI). However, there is evidence that individuals who have undergone PCI show a higher percentage of achieving the target blood pressure levels. Aim of the study was to evaluate the dynamics of blood pressure indicators in patients with stable coronary artery disease (CAD) and diabetes mellitus, depending on the fact of performing PCI.Material and methods. The study was conducted at federal state budgetary scientific institution research institute of complex issues of cardiovascular diseases as a prospective, open, not comparative. The study included 75 patients with stable CAD and type 2 diabetes who regularly received therapy for at least 1 month prior to the start of participation in this study. The majority of patients 45 (60%) are represented by males. The median age was 52.64±6.96 years. 44 patients (58.81%) had a history of myocardial infarction, and 46 patients (61.33%) had previously undergone myocardial revascularization, history of stroke had 5 patients (6.66%). All patients according to the daily blood pressure monitoring at the 1st visit had average daily blood pressure values above 130/80 mmHg. Participants were divided into 2 groups: 43 patients had indications for routine PCI at the time of inclusion in the study, 32 patients had no indications for revascularization. The groups did not have significant differences in clinical and anamnestic characteristics, and regular significant differences were noted in relation to previously undergone revascularization (46.51 and 81.25%, respectively, p=0.0022) and the SYNTAX Score (14.39±9.20 and 6.13±8.28, respectively, p=0.0001). During 4 visits (1 visit, 1 month, 3 months, 6 months), ambulatory blood pressure monitoring (ABPM) was performed using the BiPiLAB system.Results. Initially according ABPM parameters group without the alleged PCI had greater variability in systolic blood pressure (SBP) in the daytime, higher values of mean diastolic blood pressure (DBP) at night, more time index of hypertension DBP at night. After 1 month of follow — up (for the group with PCI 1 month after the procedure), there were significant differences in the average daily SBP and DBP, the index of the time of hypertension DBP in the daytime, and the average daily SBP. Patients who underwent revascularization had lower ABPM values. The previously described differences in the night time DBP and the index of hypertension time at night were preserved. After 3 months of follow-up, significant differences were observed only in the indicators of night time SAD and DBP. At the end of the study (6 months of follow-up), the groups had differences only in the indicators of mean diastolic pressure at night.Conclusion. Patients who underwent PCI for 1 month after the procedure have a significant tendency to normalize (compared to the group without revascularization) blood pressure indicators. However, over time, the effect «escapes». Most likely, the short-term improvement in blood pressure is due to a temporary improvement in treatment adherence after an invasive procedure.

Реакция семян и эксплантов льна на осмотический стресс

Abstract. Purpose. Obtaining experimental data for the development of methods for obtaining fiber flax forms resistant to stress factors (drought) by cell selection methods. Methods. The work was carried out in the Tver region in the laboratory of breeding technologies in 2017–2019. The flax varieties Varbara, Belinka, LM-98, Svetoch, Diplomat, Symfonia were used as objects of research. The seeds were obtained from the National Flax Collection Federal state budgetary scientific institution “Federal scientific center of lubya kultur”. The effect of sucrose solution on the length of the primary root was detected at concentrations of 0, 8.7, 14.9 %. To assess the energy of seed germination under osmotic stress, the concentration of sucrose was reduced and the range from 0 (control) to 9 % was considered. Results. Sucrose as a selective agent in the culture of immature flax embryos in vitro at a concentration of 5.0–7.0 % can be selective only for certain genotypes, for example, the Svetoch variety. When culturing callus tissues, using mannitol as an osmotic at concentrations of 0; 30.0; 36.4; 37.0; 37.4; 38.0 mg/l, selection of resistant callus cells with subsequent formation in meristematic foci of adventive buds and shoots can be carried out on media containing 30.0 or 36.4 mg/l osmotic. The scientific novelty of the research lies in the fact that the method for obtaining in vitro resistant explants to osmotic stress for flax is being developed for the first time.

Производственная эффективность выращивания сортов сливы в аридных условиях Астраханской области

Abstract. Purpose. The study of biometric growth indicators, the peculiarities of the passage of the main phenological phases of development, the study of early maturity, yield and commercial qualities of fruits of various varieties of plum, substantiation of the possibility and economic feasibility of their use for setting up intensive plantings in the arid conditions of the Astrakhan region. Methods. Research is carried out in the arid conditions of the Northern Caspian region, in the laboratory of fruit and horticultural crops of the Federal State Budgetary Scientific Institution “Caspian Agrarian Federal Scientific Center of the Russian Academy of Sciences”, to study the most promising variety-rootstock combinations of plums, according to the method of variety study of fruit crops for subsequent selection and recommendation of the most promising for industrial gardening. Results. Based on the data obtained and analyzed, the highest level of profitability was obtained for varieties of plum Kubanskaya rannyaya (71.4 %) with a productivity of 2.48 kg/m3 of crown volume, and a payback of 1.79 rubles. for 1 rub. invested costs, and Anna Szpet (77.3 %) with a productivity of 1.07 kg/m3 of crown volume, with a payback of 1.77 rubles. for 1 rub. investment costs. Comparing the research results, it is concluded that the Kubanskaya rannyaya variety, which is the most profitable for industrial cultivation, has a higher productivity potential and more efficiently uses the planting area in comparison with other studied varieties. This variety can be recommended for intensive gardens in the Astrakhan region. The scientific novelty lies in the fact that, despite the wide assortment of plums that exists today, for each zone it is necessary to select both varieties and rootstocks for stable and economically viable fruit production.

The Federal State Budgetary Scientific Institution "Far Eastern Scientific Center of Physiology and Pathology of Respiration" is 40 years old

The analysis of the history of creation and development of the Federal State Budgetary Scientific Institution “Far Eastern Scientific Center of Physiology and Pathology of Respiration”, its contribution to the results of research work of the Russian Academy of Sciences and the effectiveness of medical activities of healthcare institutions in the Far East region.

Anoxic brain injury due to global ischemia: analysis of three clinical cases

The article analyses three clinical cases of patients in a permanent vegetative state due to cardiac arrest and prolonged cardiopulmonary resuscitation. All patients were admitted to Federal State Budget Scientific Institution — Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia. For diagnosing and prognosis for the progression of the disease a detailed examination was held, including electroencephalography, polysomnography, positron emission computed tomography, singlephoton emission computed tomography, functional perfusion computed tomography, electroneuromyography, evoked potentials studies, laboratory studies, and other available tests. Instrumental diagnostics methods confirm the principal component that determines the level of consciousness in this type of damage — diffuse damage to the cerebral cortex. To conclude realization of the rehabilitation potential of such patients is impossible without the control of nutritional support. Amino acid profile is one of the markers and ways to control metabolic function.

ВЛИЯНИЕ КОРМОВОЙ ДОБАВКИ ПРОФОРТ НА МЯСНУЮ ПРОДУКТИВНОСТЬ ИНДЕЕК КРОССА «ХАЙБРИД КОНВЕРТЕР»

An acute problem in industrial turkey breeding is maintaining a high immune status of birds in order to increase the safety of the stock, productivity and, accordingly, the quality of meat. At the same time, the most important factor is proper bird feeding arrangement. In recent years, there has been a tendency in industrial turkey feeding to use various products that improve the birds’ digestion physiology which can replace feed antibiotics. The search for biologically active substances that can stimulate development of the natural microflora of turkeys in order to suppress pathogenic bacteria by increasing the number of bifido and lactoflora are of great interest in science and practice. Various probiotic products have similar properties. The aim of our research was to study the effect of Profort probiotic feed additive on meat productivity of turkeys of Hybrid Converter cross. A production experiment was carried out on turkeys of Hybrid Converter cross. Control slaughter of turkeys was carried out at the age of 98 days. Ten birds were selected from each group. The birds were fed for 60 days, the eed additive was given at a dose of 1 kg per 1 ton of the combined feed. Poultry processing and anatomical and morphological analysis of the carcasses were carried out in the laboratory of the research center of the Federal State Budgetary Scientific Institution “All-Russian Research Veterinary Institute of Pathology, Pharmacology and Therapy”. The results were statistically processed using Statistica v6 program . Studies showed that application of Profort feed additive had a positive effect on meat production of turkeys. The superiority of the experimental group was clearly expressed in all the studied parametres. The results obtained allow us to recommend Profort probiotic feed additive for use in turkey breeding in order to increase productivity

ИСПОЛЬЗОВАНИЕ КУЛЬТУРАЛЬНЫХ ФИЛЬТРАТОВ В СЕЛЕКЦИИ ЛЬНА IN VITRO НА УСТОЙЧИВОСТЬ К АНТРАКНОЗУ

The research was carried out on the basis of the laboratory of selection technologies of the Federal State Budgetary Scientific Institution “Federal Scientific Center of Fiber Crops” (Tver region) in 2018–2020. The aim of the research is in vitro development of new flax genotypes resistant to anthracnose, one of the most harmful fungal diseases. As a result of the research, the composition of the cultural filtrate of the anthracnose causative agent was clarified. It was revealed that toxicity of the cultural filtrates did not depend on the virulence of the strains used in the present studies, the cultural filtrates of strains 784 (highly virulent) and 780 (medium virulent) turned out to be more toxic (decay and death of radicle was observed on the 5th day in 67 - 88% of germinated seeds), less toxic are strains 793 (highly virulent) and 788 (weakly virulent) (decay and death of radicle was observed on the 5th day in 9-15% of germinated seeds). It was found that morphogenic foci were formed more actively in genotypes the morphogenic callus of which was transferred to a medium with a higher concentration of the cultural filtrate; it was shown that in the second passage, when transferring morphogenic calli from a selective medium, which contains 40 ml / L of cultural filtrate on a selective medium also containing 40 ml / L of cultural filtrate, as well as on a selective medium containing 44 ml / L of cultural filtrate, the number of formed morphogenic calli and green buds on the 14th day is significantly higher than in case of transferring on a selective medium containing 36 ml / L of cultural filtrate. Viable regenerant plants were obtained and genotypes were isolated, which retained resistance to anthracnose for three generations at a level of 50 - 60%: NO-78 x Lenok, NL-103-2 x Lenok, NL-40-1 x Lenok, NE-38 x Rosinka, NE-36 x Lenok, NE-17 x Lenok, NE-16-2 x Rosinka.

Retrospective analysis of infection of young sheep with intestinal cestodosis in the plain and mountainous zones of the Republic of Dagestan

The purpose of the research is analysis of infection with intestinal cestodosis of young sheep in the plain and mountainous zones of the Republic of Dagestan.Materials and methods. For a number of years, on the basis of the Caspian Zonal Scientific Research Veterinary Institute – Branch of the Federal State Budgetary Scientific Institution “Federal Agrarian Scientific Center of the Republic of Dagestan”, of the laboratory for the study of infective diseases of livestock and birds, we carried out studies of sheep fecal specimens from various regions of the Republic of Dagestan and tested various antiparasitic drugs.Results and discussion. It has been established that sheep cestodosis are ubiquitous in the Republic, although there are effective antiparasitic drugs, including those of domestic production. The infection extensity by Moniezia sp. in 2015 was 67.8%, Thysaniezia sp. – 12.5%, and Avitellina sp. – 6.2%. In 2016, infections continued to be recorded at a high level. In 2017, monieziosis of lambs was recorded with the infection extensity of 24.0–30.0% and with the infection intensity value of 1.5–6.4 sp. per animal. Avitellinosis was noted in 17.0–23.6 % of sheep with an infection intensity value of 1.0–4.8 sp. per animal. Cestodosis in sheep in 2019 are also found, but less frequently.

Development of new antigens for serologic diagnosis of animal trypanosomosis and their use for epizootic monitoring

The purpose of the research is developing a method for obtaining erythrocyte antigens containing and not containing Trypanosoma equiperdum and T. evansi DNA, which can later be used in serological reactions to differentiate these types of Trypanosoma.Materials and methods. The studies were conducted in the Protozoology Laboratory and the Vyshnevolotsk Branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV RAS”, as well as livestock farms of the Russian Federation and other countries using clinical, microscopic, hematological, parasitological, biomolecular and serological methods.Results and discussion. Studies carried out for the first time have shown that it is possible to use erythrocyte antigens containing the T. equiperdum and T. evansi DNA obtained after 3-fold administration to mice and rabbits of a mixture of trypanosomal antigen with addition of 1.0 ml of an adjuvant (aluminum hydroxide), and bleeding of animals at 25 to 30 days. The formed precipitate was used as an antigen for serological tests. Experiments have shown that blood for preparation of positive serum can be taken when antibodies are in titers of 1:20 in the Prolonged Complement Fixation Test, and at least 1:400 in the Indirect Hemagglutination Test and ELISA, and for negative serum when horse blood serum reacts negatively with antigens of T. equiperdum and T. evansi in the Prolonged Complement Fixation Test, Indirect Hemagglutination Test and ELISA. The test systems of the Prolonged Complement Fixation Test, Indirect Hemagglutination Test and ELISA prepared by us with antigens containing and not containing T. equiperdum and T. evansi DNA resulted in creating a universal test system (Indirect Hemagglutination Test) for differentiating T. equiperdum from T. evansi.

The results of selective breeding of blue chives (Allium nutans L) on leached chernozem of the Ob region of the Altai Territory

Perennial onion crops, due to their nutritional and taste properties, have been known and used by humans for a long time. Perennial species of onion crops are divided into edible and decorative, and people use them for these same qualities in their daily life. The group of perennial wild species of onion crops for consuming includes 18 species. These onions have a fairly high productivity and high eating experience. This group includes blue chives (Allium nutans L). In practical vegetable growing, this perennial plant is not very widespread. One of the reasons for the lack of demand for crops among producers is the lack of varieties adapted to growing conditions. The purpose of our work was to create a variety of blue chives for the conditions of the south of Western Siberia. The studies of promising clones of blue chives were carried out in 2008-2018 in the forest-steppe conditions of the Ob region of the Altai Territory. Field experiments were laid on a site located outside the selection crop rotation, at the West Siberian Vegetable Experimental Station, a branch of Federal State Budgetary Scientific Institution “FSCVG”. The objects of the research are 8 breeds of blue chives, with numbers according to the station catalog: 28, 41, 51, 72, 169, 195, 218, and 225. The subject of research is economically valuable traits and indicators of the qualitative composition of leaves. The recognized variety “Green” was taken as a standard. As a result of the research carried out on the totality of indicators, sample No. 72 was selected, which was included in the program to create a new variety in 2011. Applying the method of analytical selection and using multiple clone selection, a new variety of blue chives, named Svetloyar, was developed. In 2018 sample No. 72 was transferred to the State Variety Test, successfully passed it and, according to the results of the study in 2020, it was zoned and entered into the State Register of Plant Variety Achievements approved for use as the variety “Svetloyar”.

Experimental studies on the evaluation of ultrasonic effects on the structure, composition and nutrition of sunflower husks

Today, the bulk of the sun generated in the food and processing industry (about 70%) is supplied to agriculture unchanged, and more than 10% is not used at all. The volume of aircraft waste annually in Russia is about 3.0 million tons and the problem of their use requires serious attention, since the content of dry substances in them is about 5-10%, they are very unstable during storage, quickly sour, ferment, losing valuable components and polluting the environment. Storing them in this state is possible only for 2-3 days. Therefore, it is undoubtedly relevant and necessary to increase the degree and depth of processing of raw materials, the complexity of its use, more complete extraction of valuable components from it with the use of progressive environmentally friendly technologies. The studies were carried out at the Federal State Budgetary Scientific Institution “Federal Scientific Center for Biological Systems and Agricultural Technologies of the Russian Academy of Sciences” and the Federal State Budgetary Educational Institution of Higher Education “Orenburg State University”. In the course of the work, laboratory studies were carried out to assess the effect of cavitation treatment of sunflower husks on the chemical properties. Based on the studies carried out, it can be noted that the most pronounced changes in the chemical composition of sunflower husks are observed at pH3. So after processing in an acidic environment, the content of crude fiber is 35-40%, while the digestibility increases to 53.6%. In an alkaline medium, these values were on average 5-10% lower than in an acidic medium. The depth of interaction of cavitation treatment of sunflower husks depends on the exposure time, temperature and ultrasound intensity. It is technologically expedient to carry out cavitation treatment with the following parameters: hydromolule 1:3, medium temperature from 40 to 60 °C, time 15-20 minutes, ultrasound intensity 0.3-0.35 kW.

Dairy goats productivity using the probiotic preparation “Plantarum” in the diet

The influence of the experimental probiotic “Plantarum” on the productive and some exterior-constitutional features of dairy Saanen breed goats was studied. The preparation was developed in the microbiology laboratory of milk and dairy products of the department “Siberian Research Institute for Cheese Making” of the Federal State Budgetary Scientific Institution “Federal Altai Scientific Center of Agrobiotechnology” (FSBSI FASCA). The composition of the preparation includes pure cultures of probiotic strains of lactobacilli and propionic acid bacteria from the “Siberian collection of microorganisms”. The probiotic preparation in liquid form was introduced into concentrated feed (oats). The positive effect of the use of the probiotic “Plantarum” on the average daily milk yield of the experimental groups of fully developed (assessed by the exterior) pregnant female goats was established. A significant increase in milk yield was observed in the second and third groups by 1.66% (p < 0.05) and 3.27% (p < 0.05), respectively. With an increase in the average daily milk yield, the main physical and chemical parameters of milk (mass fraction of fat, mass fraction of protein) of the experimental groups did not decrease. There was a tendency to improve the safety of young animals in the second and third groups with an increase in the probiotic dose. At this stage of the work, the optimal dose with the maximum positive effect of introducing the probiotic preparation into the diet of experimental animals (0.8 ml/kg of body weight per day) was established. In this connection, it is necessary to conduct an additional experiment with an increase in the dose of probiotic administration when feeding it to experimental animals.

Preface

EDITORIAL PREFACE SIDDA-2020 “SUSTAINABLE AND INNOVATIVE DEVELOPMENT IN THE DIGITAL AGE” EDITORIAL PREFACE The International Scientific Conference “SIDDA-2020 “SUSTAINABLE AND INNOVATIVE DEVELOPMENT IN THE DIGITAL AGE” is held from 17 to 18 November 2020 on the basis of Autonomous Nonprofit Organization of Higher Education ANO HO “National Institute of Business” and the All-Russian Research Institute for Organization of Production, Labor and Management in Agriculture – a branch of the Federal State Budgetary Scientific Institution «Federal Scientific Center for Agrarian Economics and Social Development of Rural Areas» – «All-Russian Research Institute of Agricultural Economics».“SIDDA-2020 “SUSTAINABLE AND INNOVATIVE DEVELOPMENT IN THE DIGITAL AGE” is an annual conference.The work of the conference is aimed at providing an opportunity for scientists, graduate students and representatives of the construction industry to generalize the recent achievements results in the field of industrial and civil construction (design, organization of all types of work, construction materials, etc.), discussing promising directions for the construction industry development and establishing useful partner relationships for future interaction.List of titles ORGANIZERS AND SPEAKERS, Leading Conference Speakers are available in this Pdf.

Study of cell culture strains sensitivity to sacbrood virus

A huge number of cell culture strains obtained from one organ (tissue) of an animal, sensitive to causative agents of various infections, are known. However, a comparative characterization of various cell culture strains sensitivity to such bee viruses as SBV has not yet been carried out. This work was carried out in the Department of Cell Biotechnology and the Laboratory of Bee Diseases of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”. For the study, we took cell cultures of the African green monkey kidneys (Vero (3 strains) - Vero (strain from the FSC VIEV Collection)), Vero K (strain from the FSC VIEV Collection), Vero (strain from Germany). We have shown that all 3 of the Vero cell cultures above strains are sensitive to SBV, however, the cell model in which the most pronounced signs of CPD were revealed is the Vero K cell line. The first signs of CPD manifestation virus in the culture of Vero K cells were observed at first passage. After 6 days. screeds were found in the monolayer after infection. At the second passage of the virus after 5 days. culturing CPD manifested itself as the appearance of voids, cell strands, and rounding of the cells part.

АЛАДДИН – МОРОЗОСТОЙКИЙ СТОЛОВЫЙ СОРТ ВИНОГРАДА

The most promising way to reduce the need for fungicides in viticulture is the use of re-sistant cultivars. In this regard, an urgent task is the selection of table grape varieties with increased resistance to diseases and frost in combination with high marketability and taste. The article discusses the results of a fif-teen-year study of the promising table variety Aladdin. The variety is interspecific hybrid obtained from a saturating crossing Vostorg krasnyy × Vostorg muskatniy at All-Russian Research Institute for Viticulture and Wine-making– Branch of Federal State Budget Sci-entific Institution «FRARC». The Aladdin variety was compared with the regionalized table variety Vostorg, which is also the paren-tal form in the second generation. The viticul-ture is not irrigated, grafted, not covered, the training is fan type on trunk, the planting scheme is 3 × 1.5 m. In the variety study, the methods generally accepted in viticulture and the standard technology of cultivation of vineyards were used. The Aladdin variety is distinguished by an early ripening period, large berries, medium-sized bunches, firm pulp, attractive berry color, light muscat aro-ma. The variety has a proven genetic high re-sistance to powdery mildew and mildew, high frost resistance is confirmed by the data of agrobiological records. According to the total-ity of economically valuable traits, resistance to unfavorable biotic and abiotic environmen-tal factors, the Aladdin variety can be recom-mended for transfer to the State variety test-ing of the Russian Federation.