Federal State Budgetary Scientific Institution Research Articles

Evaluation of various varieties of peas as raw material for deep processing

The article provides an overview of the criteria for choosing pea varieties to evaluate economically valuable traits. For the effective use of peas as raw materials for deep processing, two directions can be considered: a joint increase in the content of protein and starch or the choice of varieties with the required indicators. The results of laboratory studies of processing for starch and protein isolate of 37 variety samples of peas from the collection of the Federal State Budgetary Scientific Institution «Federal Scientific Center of Legumes and Groat Crops» are presented. As evaluation criteria determining high technological properties, providing the most complete extraction of starch and protein isolate there have been selected coefficients of extraction of these components. When selecting a variety of peas, it is also necessary to consider the physicochemical properties of starch, which depend on the mass fraction of amylose. According to the results obtained, the most promising varieties for the processing of grain into starch and protein isolate are Orlovchanin, Alla, Rodnik, Sibirsky 1, Pharaon, Jaguar, Sophia. The most efficient extraction of the main processed products ‒ protein isolate and starch is established for the variety Alla, protein extraction coefficient 81.2 %, starch 90.6 %. Among the wrinkled pea varieties for industrial processing, the Amius 1241 breeding line is the most interesting, with protein isolate and starch extraction coefficients of 70.3 and 69.1 % and a mass fraction of amylose in starch of 81.5 %. It is found that the mass fraction of starch and protein in pea grains does not correlate with the extraction coefficients of protein and starch isolate during processing by two-stage extraction of protein from pea flour using the alkaline method. When processing wrinkled pea varieties, there are difficulties in separating the starch and residual protein undissolved in alkaline medium, since these pea varieties are found to have complex starch granules, which are easily crushed into small irregular and polygonal granules. In smooth pea varieties, the starch granules are oval in shape with a high percentage of large size and therefore there are no problems with separation of the starch-protein suspension. The dependence of viscosity of 3.0 % pea starch paste on the mass fraction of amylose in pea starch has been determined. The choice of pea variety for processing makes it possible to produce starch that has lifetime modification, i.e., that has the required physical and chemical properties and resistance. In addition, depending on the choice of pea variety, it is possible to predict the yield of isolate and protein concentrate.

Evaluating a newer three component herbicide Fulltime, MD for corn (Zea mays) in the central agro-climatic zone of Krasnodar Krai, Russia

Among the factors that reduce corn yield, the main one is the infestation of crops. Thus, field experiments were carried out in 2019 and 2020 on the basis of the Federal State Budgetary Scientific Institution "Federal Research Center of Biological Plant Protection" (Krasnodar). The aim of the research was to study the effect of the herbicide Fultime, MD (75 g/L mesotrione + 37.5 g/L nicosulfuron + 17.5 g/L picloram) in corn crops. The experiments were laid out in the form of randomized blocks on plots of 25 m2 in quadruple repetition. In the variants with the use of the herbicide Fultime, MD at the rates of 1.0 and 2.0 L/ha, 82-100% herbicidal effect was noted against dicotyledonous and cereal weeds without a negative effect on corn and a significant preserved grain yield (83.8 and 91.9 %) in comparison with the control (without herbicides).

Evaluating a newer three component herbicide Fulltime, MD for corn (Zea mays) in the central agro-climatic zone of Krasnodar Krai, Russia

Among the factors that reduce corn yield, the main one is the infestation of crops. Thus, field experiments were carried out in 2019 and 2020 on the basis of the Federal State Budgetary Scientific Institution "Federal Research Center of Biological Plant Protection" (Krasnodar). The aim of the research was to study the effect of the herbicide Fultime, MD (75 g/L mesotrione + 37.5 g/L nicosulfuron + 17.5 g/L picloram) in corn crops. The experiments were laid out in the form of randomized blocks on plots of 25 m2 in quadruple repetition. In the variants with the use of the herbicide Fultime, MD at the rates of 1.0 and 2.0 L/ha, 82-100% herbicidal effect was noted against dicotyledonous and cereal weeds without a negative effect on corn and a significant preserved grain yield (83.8 and 91.9 %) in comparison with the control (without herbicides).

Nutritional value of sorghum as a feedstock for poultry

Recently, many farms are increasingly using such a reserve of intensification of feed production as the cultivation of new low-spread varieties of feed crops. In this regard, sorghum, a widespread crop of southern latitudes, is attracting more and more attention. Sorghum crops due to their high heat and drought resistance, unpretentiousness to soils and low demands on nutrients, can replace cereal crops such as wheat, barley, corn, etc. The article is devoted to the study of the chemical composition and nutritional value of 15 sorghum varieties grown in the Stavropol Territory. The originator and patent holder of all these varieties is Federal State Budgetary Scientific Institution “North Caucasian Federal Scientifi c Agrarian Center”. The results of the study of these sorghum varieties in the laboratory of physiology and biochemical analysis in the Siberian Scientifi c and Research Institute of Poultry showed that the nutritional value of sorghum grain has diff erences both in varieties and in comparison with the average data specifi ed in the reference manual for poultry feeding. The varieties Zersta-38A, Knyazhna, A-63, Pharaoh, KiM, Ayushka, Athlete, Stavropol-63 and Krusta turned out to be the richest in crude protein content, but at the same time crude fat content and metabolic energy in them are less than those indicated in the methodological guide. Based on the results obtained, experimental compound feed recipes for broiler chickens and laying hens have been developed with the inclusion of varieties of different nutritional values and doses of sorghum grain with the possibility of partial or complete replacement of wheat. The conducted research is of interest because poultry is a competitor of humans in wheat consumption in order to save the traditional widely used grain crop.

To the 70th Anniversary of Academician of the Russian Academy of Sciences Vitaliy Vasil’evich Zverev

June 03, 2022 70th years have passed since the birth of Academician of the Russian Academy of Sciences, Doctor of Biological Sciences, Professor, Scientific Director of the Federal State Budgetary Scientific Institution Research I. Mechnikov Institute of Vaccines and Serums.

Влияние метеорологических условий на формирование урожайности сортов сои селекции ВНИИ сои

Abstract. The purpose is to study correlation dependence of soybean varieties yield on hydrothermal conditions of the southern zone of the Amur region. Methods. The subject of research are local recognized standard soybean varieties – Lidiya, Dauriya, and Alena. The experimental part of the work was carried out in 2017–2021 on the experimental field of the Soybean Breeding and Genetics Laboratory of the Federal State Budget Scientific Institution Federal Research Center All-Russian Scientific Research Institute of Soybean (FSBSI FRC ARSRIS). The varieties were studied in the nursery of competitive variety testing. Sowing was carried out in triplicate by the method of randomized repetitions (blocks) in the period from May 19 to May 21 using a SN-P-16 seeder. The plot area is 40.5 m2. During the growing season, cultivation, manual weeding, phenological observations and visual assessments were carried out. The plots were harvested by continuous threshing with a Sampo 130 combine. The yield of varieties was determined in terms of standard moisture content (14 %). Results. In the course of the yield data analysis of varieties depending on weather conditions it was found that 2019 and 2020 were excessively wet (HTF – 2.6; 2.4), optimum conditions developed in 2017 (HTF – 1.7). The highest average varietal yield (26.5) over the years of research was noted in 2017, the lowest (23.4 c/ha) – in adverse meteorological conditions of 2018, due to drought period of the early plant growth and heavy storm rainfall afterwards. It was found in the course of research that formation of the Lidiya soybean variety yield is mainly influenced by the effective heat sum during periods of sprouting – flowering and flowering – ripening (r = 0.51 and –0.53). The productivity of the Dauriya variety is more dependent on the air temperature (r = –0.61) and total precipitation, and HTF of flowering – ripening periods (r = 0.62; 0.67). The Alena soybean variety showed a fairly close correlation dependence of the productivity on the effective heat sum of the periods of dropping – sprouting and flowering – ripening (r = –0.87 and –0.68), and the amount of precipitation and HTF of the period sprouting – flowering (r = –0.64 and –0.60 respectively). Scientific novelty. Special aspects of meteorological effects on the yield of soybean varieties of various ripeness groups have been established; the correlation between them have been determined. It has been found that all studied varieties are highly adapted to changing weather and climatic conditions of the Amur Region.

The automated cell counter for the analysis of morphological characteristics and the quantitative estimation of starch granules in different potato varieties

Relevance. Potato starch is widely used in the food, textile, perfume and pharmaceutical industries. The starch content and the size of starch granules in potato tubers are varietal characteristics (Solanum tuberosum L.). The knowledge of morphological properties of starch plays a key role in the technologies for the production of consumer and industrial goods.Materials and methods. The studies were carried out in 2020-2021 at the experimental base of Federal State Budget Scientific Institution “Federal Scientific Center of Agricultural Bio-technology of the Far East named after A.K. Chaiki” (Primorsky Krai). The object of the research were 11 varieties of different maturity dates. Morphological structure of starch granules was evaluated by image analysis on Countess II FL automated cell counter.Results. The varieties studied had predominantly rounded and oval pellets regardless of maturity and genetic origin. Differences in starch granule size depending on tuber size and ripeness group were noted. Early maturing varieties had granule size less than 20 microns regardless of tuber fraction. The exception is the variety Queen Anne, in small tubers of this variety starch granules larger than 20 microns is 67.86%. It was noted that the proportion of medium and large grains increased in varieties with a longer growing season. For example, the variety Augustin medium-ripening had a proportion of medium and large grains in the large and small tuber of 47.72 and 41.48%, respectively. The maximum number of grains larger than 20 microns was observed in the mid-late varieties Kazachok (63.77%) and Smak (92.22%).Conclusion. The method using a cell counter and subsequent processing of microphotographs of starch granules is an accessible, economical, simple and effective approach to phenotyping potato varieties and hybrids of Solanum tuberosum L. by physical and chemical parameters of starch. This method can be used for accelerated analysis of a large number of samples on a limited amount of natural material, including in field and farm laboratories.

Growing the planting material of juzgun leafless in the Astrakhan region

. In the Astrakhan region, land desertification occurs mainly in the form of deflation and salinization. This process is characterized by the degradation of the vegetation cover, the development of erosion processes and the growth of open sand areas. It is possible to stop the desertification of territories by creating forest protection belts and phytomelioration of degraded phytocenoses. As part of scientific work at the Federal State Budget Scientific Institution "PAFSC RAS" in the period from 2019 to 2021 research was carried out on the cultivation of planting material juzgun of various phenotypes, for use in phytomelioration of arid pastures. As part of the experiment, a leafless dzhugun shrub of various phenotypes (Astrakhan and Kazakh) was sown on planting material under rainfed and irrigated conditions. When growing seedlings on irrigation, the method of drip irrigation was used. According to the results of the study, the following was revealed: the germination of seeds of leafless juzgun on a rainfed land was higher than on irrigation by 1.3-9.9%. The germination of seeds of the Astrakhan phenotype turned out to be 2.5 times better than the Kazakh one on a rainfed land, and on irrigation, the seeds of the Kazakh phenotype had single shoots. The height of plants by the end of the growing season on irrigation exceeded the rainfed variant by 0.35-0.85 m. At the end of the growing season (September-November), depending on the amount of soil moisture (50-60% of HB), after autumn rains, all planting material was planted on arid natural pastures. Seedlings grown on rainfed crops had a survival rate of 37.7 - 49.6%, and on irrigation - 43.4-45.4%.

Reproductive cycles and spermatogenesis in male sterlet in industrial fish farming

With the advances made in the aquaculture industry, the role of males is becoming increasingly important, since their reproductive capacity determines the outcomes of artificial breeding. The key factor here is the developmental asynchrony of sex cells inherent in sturgeon males, which helps retain spawning readiness in males for longer periods of time without loss of quality of reproductive products produced by them. The paper presents the findings of the studies performed at the Konakovo Division of the Branch for Freshwater Fisheries of the Federal State Budget Scientific Institution “Russian Federal Research Institute of Fisheries and Oceanography” (VNIIPRKH), in a flow-through industrial farm and recirculating aquaculture system (RAS). These studies have confirmed the possibility to control spermatogenesis in sturgeons by changing the temperature during the prespawn period, thus improving the productivity of fish farms by prolonging the breeding season. The histological analysis of sterlet spermatogenesis presented in the paper offers an insight into annual reproductive cycles, natural developmental asynchrony of sex cells, and successive transformations of mature and overmature sperm in cysts and excretory duct. Such phased analysis of the development of sex cells, depending on environmental factors (the effect of water temperature) and considering the patterns of spermatogenesis, made it possible to propose the methods to control spermatogenesis in mass spawning. The design of recirculating aquaculture systems makes it possible to control the environmental conditions and spermatogenesis, and promote spawning in sturgeon males in due time, thus ensuring high-quality reproductive products whenever the farm need them. The histological analysis has not only offered an insight into the gonadal maturity parameters, but also, with partial cyst emptying, has allowed detailed characterization of the development of sex cells in spermatogenesis. Understanding the reproductive capacity of males under various housing conditions will allow defining the timing and duration of spawning, i.e., the prespawn, spawning, and postspawn periods of the annual cycle in sterlet.

Bocconia cultivation in the Belgorodsky region

Bocconia is an evergreen plant belonging to the poppy family, and has been used in folk medicine since ancient times. The unique feature of plume poppy to cure various diseases can be explained by the alkaloids, sanguinarin and heleritrin, available in its green component. In the period of 2019 – 2021 in the Belgorod branch of the federal state budgetary scientific institution «All-Russian Institute of medicinal and aromatic plants» field studies were carried out to study the effect of AgroMaster complex fertilizer of grade 13-40-13 with microelements on the productivity of plume poppy. Complex fertilizers are an important reserve for increasing the productivity of crops, including medicinal and aromatic plants [4, p. 273]. Along with the main application of mineral fertilizers, a significant role belongs to plant fertilization, especially complex fertilizers with trace elements. The main components of complex fertilizers are macrofertilizers (nitrogen, phosphorus, potassium, and others), which are necessary for proper growth and development of any crops. Auxiliary composition of complex fertilizers includes such important chemical elements as zinc, copper, manganese, and others. All of the above makes the use of complex fertilizers an indispensable method of increasing the productivity of cultivated crops [6, p. 194; 7, p. 138; 11, p. 55]. The objectives of the study are to study the peculiarities of adaptation, growth processes and yields of bocconia in specific agro-ecological conditions. Variants of field study included different doses of AgroMaster 13-40-13 fertilizer (from 2 to 5 kg/ha) with single and double treatment of plants to identify the optimal value. The greatest intensity of growth processes, as well as the growth of leafy mass has been found in the double treatment of plants at a dose of 2.0 + 2.0 kg/ha. Variant with maximum dose of fertilizer is 5,0 kg/ha at double treatment provided insignificant yield increase – 0,1 t/ ha against the background of the best variant.

Improved methods of cultivating dining beet in arid conditions of Northwest Caspian Sea

For the first time, in the conditions of light chestnut soil of the Astrakhan region, a study was conducted on the influence of extracorneal treatments with modern growth stimulating drugs aimed at increasing the yield of the studied crop during drip irrigation. A field experiment on the cultivation of various varieties and hybrids of table beets, carried out at the experimental irrigated area of ? ? the Federal State Budgetary Scientific Institution "PAFNC RAS" during 2019-2021. showed that the largest commercial yield of 66.9 tons/ha was obtained on the option using extrabort treatment with Aminovit fertilizer. Net income per 1 hectares under this option was 644.9 thousand rubles, the profitability of production of 1 ton of products amounted to 179.8%.

Variety study of home plum for intensive plants in arid conditions of the Northern Caspian region

The results of biometric indicators of growth, features of the passage of the main phenological phases of development, analysis of precocity, yield and commercial qualities of fruits of various plum varieties are presented. The studies were carried out on the irrigated plot of the orchard of the Federal State Budgetary Scientific Institution "Caspian Agrarian Federal Scientific Center of the Russian Academy of Sciences", which was founded in 1993. The difficult climatic conditions of the region require the selection of varieties, first of all, with increased adaptive potential. As a result of the research, it was found that the Renklod Altana (3.1 m) and Mechta (3.1 m) varieties had the highest tree height. All studied varieties began to bear fruit in the 3rd year. Stable fruiting was observed in all varieties in the 5th year of vegetation. The highest yield was obtained in the variety Tatyana - 8.6 kg/tree.

INFLUENCE OF MINERAL COMPOSITION OF SELECTIVE MEDIUM ON FLAX MORPHOGENESIS IN IN VITRO CULTURE

The studies were carried out on the basis of the laboratory of breeding technologies of Federal State Budgetary Scientific Institution "Federal Scientific Center of Bast Crops" (Tver region) in 2016, 2018, 2021. The purpose of the research is to determine appropriate mineral composition of the selective medium for effective in vitro selection for resistance to anthracnose. Various mineral composition media were studied. It was found that it is possible to use nutrient media Gamborg, MS, SH-2, which do not contain vitamins, chelate complex and phytohormones, in order to obtain cultural filtrates of fungus strains - the causative agent of flax anthracnose. The culture filtrates obtained on the basis of these media were highly toxic and it was possible to use them for in vitro selection. It was noted that the callus formed on the basis of hypocotyl segments had a higher ability for morphogenesis compared to the callus formed on the basis of immature embryos. As for selective medium consisting of mineral salts of Gamborg medium and cultural filtrate, the proportion of formed morphogenic callus was the smallest in all genotypes taken in the study and amounted to 0.1–6.8%. Mineral composition of the selective medium and the morphogenetic potential of the genotype influenced the formation of morphogenic callus based on primary callus. Depending on the genotype, the proportion of morphogenic calli formed on Gamborg medium + CF was 0.1 - 2.2%, on MS medium + CF - 3.3 - 8.0%, on Sh-2 medium + CF - 3.4 – 8.1%, respectively. Mineral composition of the selective medium had no significant effect on formation of morphogenic cells based on transplanted callus. The proportion of morphogenic callus was 3.0–5.2% for Lenok variety; 3.0 - 5.4% - Rosinka; 0.1 - 4.3% - Zaryanka; 3.1 - 5.5% - for L 2053-5-11 line; 6.1 - 7.4% - L 957-8-7. The breeding lines L 2053-5-11 and L 957-8-7 had a high morphogenetic potential.

ADVANTAGEOUS SOURCES OF BREEDING AND VALUABLE TRAITS AMONG GRAPE VARIETIES OF ANAPA AMPELOGRAPHIC COLLECTION

Genetic collection of Anapa Zonal Experimental Station of Viticulture and Winemaking includes samples of grape culture of various ecological and geographical origin, which are successfully used in breeding work of this experimental station and other scientific institutions. Active search for new sources and donors of economically valuable traits and resistance to biotic and abiotic stressors is carried out in ampelographic collection of genetic resources of Anapa Zonal Experimental Station of Viticulture and Winemaking. This article contains main results of scientific research over the past three years related to the study and identification of new sources of economically valuable traits and resistance to biotic and abiotic environmental factors among the varieties of the ampelographic collection of Anapa Zonal Experimental Station for Viticulture and Winemaking - a branch of the Federal State Budgetary Scientific Institution of the North Caucasus Federal Scientific Center of Horticulture, Viticulture, Winemaking. The objects of the research were grape varieties of various ecological and geographical origin from the gene pool of Anapa ampelographic collection. The study used traditional and improved breeding programs and methods. In the course of long-term research work, 15 sources of selection-valuable traits were identified among the grape varieties of Anapa Zonal Experimental Station of Viticulture and Winemaking, which are used in crosses to create new varieties. These source varieties are annually investigated for phenological and agrobiological characteristics, which have consistently high parametres. As for table grape direction, such varieties as Strashenskiy, Doch Nimranga, Pamyati Dombkovskaya, Arcadia and Olimpiada have the highest fruiting coefficient and yield. Source varieties that annually have high parametres of sugar accumulation - an important feature for winemaking - Bastardo, White Muscat, Rkatsiteli Magaracha, Pierrel are actively used in hybridization to produce new high-quality grape varieties.

INCREASE OF SELECTION EFFICIENCY OF FLAX INITIAL MATERIAL IN PRIMARY SEED PRODUCTION

The studies were carried out on the basis of the laboratory of breeding technologies of the Federal State Budgetary Scientific Institution "Federal Scientific Center of Bast Crops" (Tver region) in 2019-2021. The purpose of the research is to study selection effectiveness of fiber flax plants for a new trait - height at various seeding amounts in the selection nursery. It was established that initial testing with subsequent removal of plants that were not included in the interval of typical height (± 10% of the average value) allowed to ensure a higher uniformity of typical plants in terms of fiber content compared to the control in the stalk, when sowing 200 viable seeds per meter of row in the selection nursery, the highest yield of renewed seed material (91.8 g/m²), it also allowed to reduce labor costs. Studies showed that selection of fiber flax plants by height provided an increase of seeding energy in case of sowing of 150 and 200 viable seeds by 14-15%, seed strength by 20.8-27.3%, and 1 cm of seed germination by 7.0 - 21.4% compared with the control variant. However, the studied methods of plant selection did not affect mass formation of 1000 seeds. According to soil control data, when testing plants for a new trait, their high uniformity was achieved in terms of the main varietal trait - fiber content in the stem, which was characterized by variation coefficient at a level of 3.0-3.5% with 3.0-3.1% in control. A good evenness of plants was also revealed by another varietal trait - height. Variation coefficient for this trait was 4.3-5.5%. The effectiveness of selection method of fiber flax plants by a new trait-height is confirmed by the results of its verification. When checking the method, the cost reduction for work performance on selection and testing of plants by height amounted to 27% compared to the control.

Updating the rates of output of pollock processing products based on statistical analysis of experimental data. Message 2. Updating the rates of walleye pollock processing products in the West Bering Sea, East Kamchatka, North and South Kuril zones

The purpose of this study is update the norms for the output of walleye pollock processing products for the Far Eastern fishery basin based on a statistical assessment of the results of experimental control works aimed at improving the fishing regulation system in terms of verifying actual catches. Methods used: experimental and control work was carried out according to the «Methods for determining the consumption rates of raw materials in the production of products from hydrobionts». 145 experimental and control works were carried out, the mass of raw fish directed to their implementation was 21499.4 kg. Calculations of the results of experimental and control work using the «Complex of programs for processing the results of experimental control works in the production of frozen products from raw fish (except for sturgeon and salmon)». Novelty: it has been established that the use of modernized fish cutting equipment or new generation machines can increase the yield of headless walleye pollock by 3,8 %, and output skinless and boneless walleye pollock fillets in season «B» increased by 2,9 %. For the first time, the output of skinless, boneless fillets from walleye pollock in season «A» was established, which amounted to 26,9 %. Result: this article is a continuation of the great work carried out by the Federal State Budgetary Scientific Institution «VNIRO» to update the rates of output from walleye pollock. In the first message, the rates of output from the Sea of Okhotsk pollock were considered, in the second message the data on the output of production from the West Bering Sea, East Kamchatka, North and South Kuril zones. Practical significance: the developed rates for the yield of walleye pollock processed products in 2020 included in new collection of «Basin rates for waste, losses, finished product yield and raw material consumption in the production of frozen and fodder products from fish of the Far East region».

Inventive Activity of Educational Institutions Subordinate to the Ministry of Agriculture of Russia

The general level of inventive activity of agricultural universities subordinate to the Ministry of Agriculture of Russia is revealed. The current state and main directions of inventive activity of agricultural educational institutions are shown. The information services of the industry databases (DB) of the Federal State Budgetary Scientific Institution “Rosinformagrotekh” for the formation of a digital environment for monitoring and accounting for the results of intellectual activity (RIA) in the implementation of the state task in the field of science are considered.

Сравнительная оценка водных экстрактов прополиса, приготовленных разными способами

Abstract. In the course of the study, an updated database of the content of biologically active substances in aqueous extracts of propolis prepared at different temperatures and in different ways is presented. The purpose of the work was to select the optimal modes of preparation of aqueous propolis extracts, to determine the content of biologically active substances in freshly prepared propolis extracts and during storage. The studies were carried out in the laboratory of the Federal State Budgetary Scientific Institution “FNC of beekeeping”. The relevance of the work lies in the development of methods for extracting aqueous extracts of propolis at different temperatures (t = 20 °C, t = 93 °C), the use of ultrasound. Scientific novelty for the first time biologically active substances were determined by the method of determining antioxidant activity. Methods. The yield of extractives (mass fraction of solids) was determined by drying to constant weight according to GOST 28886-90. Determination of unsaturated compounds was performed according to GOST 28886-90. The determination of the hydrogen index (pH) was performed according to the Pharmacopoeia (1987) in our modification GOST 28886-90. Determination of flavonoid and other phenolic compounds was performed by photometry, according to GOST 28886-90 (total flavonoid compounds). Determination of flavan, flavanones, flavonols was carried out according to the method presented in the “Guidelines for methods of quality control and safety of biologically active food supplements” R 4.1.1672-0, 2004 in terms of routine. The antioxidant activity of propolis aqueous extracts was assessed by a method based on the reaction of KMnO4 in the presence of 0.24 M H2SO4 with the studied propolis solution, followed by conversion to quercetin in 1 ml or 1 g of the drug (patent 2170930). Results. The amount of solids during storage in the aqueous extract of propolis (method 1) decreased from 0.23 to 0.15 %; in an aqueous extract prepared using ultrasound (method 3) increased from 0.21 % to 0.23 %. The amount of solids in propolis aqueous extracts prepared by methods 2 and 4 decreased during storage from 0.55 to 0.49 % (method 2) and from 0.6 to 0.59 % (method 4).

DEVELOPMENT OF THE ALL-UNION INSTITUTE OF HELMINTHOLOGY IS THE WAY OF GREAT ACHIEVEMENTS (to the 90th anniversary of creation)

April 7, 2022 All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution "Federal Scientific Centre VIEV" celebrates the 90th anniversary of the establishment of an independent scientific institution in the field of helminthology in Russia. For the first time in the world, a specialized institute was created, which included all areas of helminthological science. This event was preceded by many years of hard and purposeful work of the founder and first director of the All-Union Institute of Helminthology Konstantin Ivanovich Skryabin, his students and associates. In this work, we have tried to show this way of the formation of scientific thought, the development of scientific methods, the search for and training of helminthologists, the deepening of work, the formulation and solution of a large number of problems. K. I. Skryabin successfully applied the features of the socialist system for the systematic control of helminthiasis in animals, humans and plants, based on a solid foundation of theoretical training. At present, the institute continues to work successfully in the field of veterinary and agronomic parasitology

Evaluate of tomato growing in the conditions of subirrigation aeroponics at Fitopiramida greenhouse

Aeroponics and hydroponics technologies are one of the most advanced today. The aim of this work is to select and assess the assortment of determinant tomatoes for suitability for cultivation under the conditions of this technology. The study was conducted on the crop of two specific hybrids of different foliar dressings, as well as the assessment of the level of interaction «variety-option of feeding» based on the statistical analysis of the results. The article discusses the features of two methods of the aeroponics and Fitopyramida soilless technology in the context of solving the problems of resource and energy conservation in the production of vegetables. It is applied in agriculture to plan the several activities and missions properly by utilizing limited resources with minor human interference. The aim of this work is to evaluate the effectiveness of non-root top dressing and to identify the optimal habitus and other characteristics of the determinant tomato hybrid for the “Fitopiramida” technology. A greenhouse research project was conducted at All-Russian Scientific Research Institute of Vegetable Growing - a branch of the Federal State Budgetary Scientific Institution “Federal Scientific Center of Vegetable Growing” (VNIIO - a branch of FGBNU FNTSO). This study was revealed that 2 foliar dressing at a dose (N: P: K 10:54:10) 2.5 g/l + Maxifol 2g/l ) have a significant impact on productivity and yield determinant hybrids of tomato Flame F1 and Rosana F1, providing yield increase in comparison with the control by 7.87 kg/m2 and 2.52 kg/m2, respectively. Among the investigated options for the use of mineral fertilizers, the most maximum and reliable results were obtained in the 3NPK option; on the basis of statistical processing, the optimal variant of the interaction «variety-level of mineral nutrition» was established - hybrid F1 Rozanna against the background of 3NPK.