Cream Preparations Research Articles

Preparation and Evaluation of Herbal Cold Cream with Incorporated Curcuma longa

Preparation and Evaluation of Herbal Cold Cream with Incorporated Curcuma longa

Formulasi Lip Cream Ekstrak Etanol Biji Buah Pinang (Areca catechu L.) sebagai Pewarna Alami

Natural dyes are a dye that can be produced from various types of plants that produce natural colors that can be obtained from existing parts in plants such as leaves, fruit, stem, flowers and seeds. Lip cream is one of the decorative cosmetics used to lubricate lips so that it is not easily dry and cracked. Lip Cream as a lip dye that can be used safely with the dye of areca nut extract (Areca catechu L.). Pinang fruit seeds as natural dyes contain Tanins and phenolic compounds. This study aims to determine the characteristics and stability of areca nuts (Areca catechu L.) as a natural dye in a lip cream preparation. In making lip cream there are several ingredients used in it such as Bees Wax, Whith Wax, Cethyl Alcohol, Dimeticone, Kaolin, Castrol Oil, Titanium Dioxide, Kaolin, Toko, and Methil Paraben. Researchers used BUA Pinang seed extract as a natural dye in a lip cream preparation with a concentration of 15%, 25%, 50%, and tested physical quality, namely organoleptic test, pH, homogeneity, and for its effectiveness, namely attachment, and the power of spread, as well as Stability test. Based on research and results of evaluation tests on the field of lip cream fruit seed extract areca nut all formulations are not significantly different in the evaluation of stability.

Testing Of The Cream Formula Turmina Right Extract Against The Inhibition Of The Development Of Melanoma Cells

Cancer is one of the biggest causes of death in the world and is still a health problem that still cannot be found the right treatment to overcome it. One of the cancers that cannot be ruled out is skin cancer, which occurs as a result of being exposed to direct sunlight for a long time and often without using skin protection or sunscreen. Cancer treatment that is currently being carried out is surgical therapy, chemotherapy which has adverse side effects for patients such as therapy that not only kills cancer cells but also healthy cells. The current therapy needed is the formulation of topical preparations that can be used to inhibit the growth of cancer cells. This study aims to make a cream preparation from turmeric extract to inhibit the growth of melanoma cells. This research was made experimentally by formulating turmeric rhizome extract into cream preparations at a concentration of 0.5%; 2.5% ; 5% ; 10% and 20% were tested against melanoma cell inhibition in vitro. Melanoma cell inhibition testing was carried out using the MTT Assay method by looking at the IC50 value of turmeric extract cream in inhibiting the growth of melanoma cells. The results showed that 20% turmeric rhizome extract cream was the formulation that gave the best IC50 value of 524.42 ± 5.2 g/mL.

FORMULATION AND EVALUATION OF BREAST MILK ETHANOL EXTRACT FRUIT MEAT (Psidium Guajava L.) BY USING VARIATIONS OF EMULGATOR CONCENTRATION

Preparations cream is a semisolid dosage forms containing one or more ingredients dissolved or dispersed in the base material which one of the plants that is used as a face cream is guava, because it contains vitamin C which is quite high and has the potential as an excellent antioxidant. This study aims to determine the effect of emulsifier concentration on cream preparations and which formulas show stable results.
 This research is an experimental study with a posttest only design. The population in this study was guava fruit (Psidium guajava L.) from guava trees in Dulomo Selatan Village, Kota Utara District, Gorontalo.
 The results showed that in formula I (F1) and formula III (F3) there was a rancid odor, for texture and appearance there had been a change from formula I (F1), namely soft and dense texture and formula II (F2) and formula III (F3). to a soft and fluid texture. The result of measuring the pH of cream preparations in formula 1 (F1) is 7, formula (F2) is 7 and formula (F3) is 7, the test for spreadability of cream preparations in Formula 1 (F1) is 5.25 cm, Formula 2 (F2) 4 .5 cm and Formula 3 (F3) 5.25 cm, the centrifugation test for cream preparations in Formula 1 (F1), Formula 2 (F2) and Formula 3 (F3) separated for 2 hours.

New Hair Growth Cream Formulation with Cocoa Pod Peel (Theobroma cacao L.).

Our previous study verified that the waste skin of cocoa (Theobroma cacao L) fruit or waste cocoa pod husks had the efficacy to overcome hair loss or alopecia. This study aims to determine the formula and activity of hair cream of cocoa pod peel water fraction, which is effective in stimulating hair growth. Activity testing uses the modified Tanaka method. The results showed that the cocoa husk wastewater fraction could be formulated into hair cream, but there were changes in viscosity and pH after the freeze-thaw test, but still within the allowed limit. The hair cream water fraction gel stimulated hair growth activity based on the hair length data with a significant difference in concentration of the preparation. The best activity in hair cream preparation was at 12.5% concentration. In addition, there were no signs of irritation to the rabbit's skin where hair cream preparation was applied. The results of this study indicated that cocoa fruit peel cream can be used for antialopecia treatments.

Formulasi dan Uji Aktivitas Antioksidan Krim Biji dan Ekstrak Etanol Daun Jambu Biji (Psidium guajava L.) sebagai Body Scrub

Ethanol extract of guava leaf (Psidium guajava L.) contains flavonoids and tannins that are potentially as an antioxidant that may inhibit the activity of free radicals.The aim of this study is to formulate a body scrub cream from ethanol extract of guava leaf (Psidium guajava L.) and test their antioxidant activity.This study used three variations of concentration of ethanol extract 70% of guava leaf (Psidium guajava L.) i.e 1,5%, 2% and 2,5%.Body scrub creams are formulated into O/W type of creams by using guava seeds as a scrub.Evaluation of preparations included organoleptic examination, homogeneity, pH, cream type, stability test, particle size distribution, dispersion test and antioxidant activity test using DPPH method. The evaluation results showed that the physical properties of cream preparations provided good results and meet the requirements of cream preparations.The results of the antioxidant activity test found that the values of IC50 for each formula was F0 (without extract) 379,58 µg / mL, F1 (1,5%) 150,78 µg/mL, F2 (2%) 137,26 µg/mL and F3 (2,5%) 125,84 µg/mL.It can be concluded that body scrub creams of ethanol extract 70% of guava leaves (Psidium guajava L.) has the potential to be developed as a cosmetic preparation that can inhibit free radicals.

Anti-Aging Face Cream Preparations Made from Collagen Membrane Chicken Eggshell (Gallus gallus domesticus) Combined with Bee Honey (Apis mellifera)

Handling waste such as chicken egg shells requires innovation to be able to provide added value as a sustainable product. This study aims to extract collagen from chicken egg shell membranes combined with bee honey as an anti-aging face cream. This material has a very high model accuracy value (97.89%) which has the potential to inhibit aging due to Matrix metalloproteinase-1 (MMP-1). This research was carried out by extracting eggshell membrane collagen, making cream combined with bee honey, analyzing product properties, and interpreting virtual data. The results show that in 100 g of face cream formulation with active ingredients of chicken eggshell membrane collagen extract 4.1206% and bee honey 5.0251%; 77.0926% water; 0.0062% ash; 1,8690% protein; 0.0058% fat, the viscosity value was 65.94 dPas, and the pH was 7.08. Thus, this product did not irritate the skin of the experimental animals. The average value of attribute assessment (such as aroma, consistency, texture, stickiness, homogeneous sensation, color, appearance, itching, erythema, washing power, response after washing) was well received by the panelists with a score of 1-3,9 and the highest total achievement bacteria 25 CFU/g and yeast-mold 16 CFU/g met the skin moisturizer quality requirement (SNI 16-4399-1996) which should not exceed 102 CFU/g.
 Key words: collagen; Matrix metalloproteinase-1; honey; extraction; organoleptic

The Effectiveness of Pandan Wangi Leaves (Pandanus Amaryllifolius Roxb.)Body Scrub Formulation in Smoothing the Skin

This study aims to formulate ethanol extract of pandan wangi leaves into a cream body scrub preparation and examining the effectiveness of body scrub in smoothing the skin by analyzing the moisture and evenness of volunteers' skin. This research was carried out by sample processing, extract making, formulating cream body scrub preparation by evaluating its consistency, color and odor (organoleptic), homogeneity, pH of preparation, spreadability, emulsion type, irritation test, stability and effectiveness test using skin analyzer. Cream scrub preparations were made with several concentrations, namely 1%, 2% and 3%. The results of the study showed that the ethanol extract of pandan wangi leaves (Pandanus amaryllifolius Roxb.) could be formulated in cream scrub preparations which show homogeneous preparations, pH ranging from 5.4-6.0, met the requirements for spreadability, was an oil in water emulsion type, did not irritate the skin and the preparations were stable during the accelerated stability test with cycling test method. Furthermore, the cream scrub preparations of Pandan wangi leaf extract can increase moisture and improve skin smoothness until 31.48% and show a significant different with another group test (p<0.05).

EKSTRAK ETANOL DAUN SUJI (Pleomele angustifolia) TERENKAPSULASI NANOPARTIKEL KITOSAN DALAM SEDIAAN KRIM TABIR SURYA DENGAN VARIASI TWEEN 80-SETIL ALKOHOL

ABSTRACTAntioxidants in the ethanolic extract of suji leaves (Pleomele angustifolia) have the potential as a sunscreen. The use of chitosan in the nanoparticle system is able to protect the degradation of flavonoids due to the influence of temperature and light. Tween 80 and cetyl alcohol play a strategic role in the formation of cream preparations. The purpose of this study was to determine the characteristics of sunscreen cream with variations of Tween 80-cetyl alcohol containing the active ingredient of ethanol extract of suji leaves encapsulated in a nanoparticle system. Chitosan nanoparticles of suji leaf ethanol extract (KEEDS) were prepared using ultrasonic agitation. KEEDS nanoparticles were made into cream preparations with variations of Tween 80-cetyl alcohol (T80:SA) FI (3:3)%, FII (3:10)%, FIII (10:10)%. The formed cream was observed for organoleptic, homogeneity, type of cream, and measured the value of pH, spreadability, adhesion, viscosity, and sunscreen activity using a UV-Vis Spectrophotometer at a wavelength of 290–320 nm. Organoleptic data, homogeneity, pH and cream type were analyzed descriptively, while the spreadability, adhesion, viscosity and sunscreen activity were analyzed using ANOVA with 95% confidence limit. KEEDS nanoparticle cream visually looks homogeneous, with a brownish white color, soft texture, and the aroma of suji leaves. The pH value of the preparation is 4.74-4.88, the type of cream is O/W. The variation of the T80:SA combination resulted in significant differences in the values of dispersion, adhesion, viscosity and sunscreen activity between the three formulas (sig < 0.05).Keywords: cetyl alcohol, chitosan, cream, leaves Suji (Pleomele angustifolia), sunscreen, Tween 80

Effect of Process and Formulation Variables on the Structural and Physical Properties in Cream Cheese using GDL Acidulant

We report on the properties of analogue cream cheeses prepared using glucono delta-lactone (GDL) acidulant, notably the impact of particular processing and formulation variables, (homogenisation pressure, coagulation pH and temperature, and stabiliser level) on cream cheese physical, material and microstructural properties. Protein–protein and protein-fat interactions were seen to be the primary structural contributors to the physical properties of cream cheese. Cream cheese microstructure and its properties demonstrated well-defined correlations to specific and controllable processing elements within the manufacturing process, showing significance in interactions between parameters in multivariable linear regression analysis (P < 0.05). Summarising the effect of processing variables on key cheese properties, we observed that a progressive reduction in fat particle size of cheese milk arising from increasing homogenisation pressures was seen to increase the total surface area of fat that could be incorporated into the curd during coagulation. The greater extent of fat-fat and fat-proteins interactions during coagulation provided a reinforcing effect on the microstructure of the final cream cheese, with a corresponding increase in compressive fracture stress, shear storage modulus (G′) and shear loss modulus (G″). In terms of other processing variables, cream cheese firmness was also observed to progressively increase through lowering of coagulation pH from 5.13 to 4.33. Increasing coagulation temperature from 58 °C to 78 °C similarly caused an increase in cheese firmness. Finally, increasing the levels of added stabiliser were shown to correlate with increasing cheese firmness. Similar correlations could be observed in relation to physical properties, notably forced expressible serum separation. This model cream cheese preparation method has provided a useful model system for relating food structure to material and functional properties. In addition, it has the advantage of being able to rapidly screen many formulation and process variables because it is faster than the traditional cheesemaking. This study showed that the adjustment of process and formulation variables, either in isolation or in combination, in the manufacture of cream cheese can significantly influence the final material and textural properties of the product, thereby enabling controllable functional attributes capable of meeting different customer needs.

Uji Hedonik dan Daya Simpan Sediaan Salep Ekstrak Etanol Umbi Hati Tanah

The Tanah Hati plant is one of the medicinal plants that have benefits as traditional medicine and is used by the people of the city of Palangka Raya, Central Kalimantan Province. Empirically this plant tuber is believed to treat wounds, postpartum wounds, malaria, and diarrhea. The ethanol extract of the Hati Tanah tuber has been made into an innovative pharmaceutical preparation in the form of an ointment. Hati Tanah tuber ethanol extract ointment is intended to inhibit wound infection caused by the bacteria Staphylococcus aureus. The purpose of this study was to determine the level of preference through hedonic tests and shelf-life stability of pharmaceutical preparations of ethanol extract ointment from Hati Tanah tuber with a hydrocarbon base and a water-soluble base. The method used in this study is a method non-experimental with steps in the form of hedonic tests and shelf-life stability. The assessment that becomes a reference in the hedonic test includes aroma, shape, and color. To test the stability with the shelf-life indicator using the variable storage time after the measured variable. The stability test was carried out for 2 weeks, tested from day 0 to day 14 by observing changes in aroma, shape, and color of the ethanol extract of the liver soil tuber ointment. Based on the results of research that has been carried out on 20 panelists and 2 weeks of storage, it was found that the pharmaceutical preparations of the ethanol extract of the liver tuber in a water-soluble base were preferred in terms of aroma and shape. Meanwhile, the preparation of hydrocarbon-based ointments is preferred in terms of the color of the preparation. In the shelf-life stability test, it was found that the pharmaceutical preparations of the ointment with ethanol extract of the liver tubers on a hydrocarbon basis showed a change in the dosage form on the 7th day, which was watery at the top of the ointment. While the water-soluble base ointment remained stable for 2 weeks of storage.

Formulasi Sediaan Krim Antiinflamasi Ekstrak Etanol Daun Rambusa (Passiflora foetida L)

Rambusa (Passiflora foetida L) is a medicinal plant that is widely found in various regions in Indonesia, including in Central Kalimantan. Rambusa is empirically used as an antibacterial and anti-inflammatory. Previous research has shown that the content of Rambusa (Passiflora foetida Linn) leaves from Central Kalimantan is one of the steroids which has anti-inflammatory properties. The purpose of this study was to determine whether the ethanol extract of rambusa leaves can be processed into anti-inflammatory cream preparations and to determine the results of physical tests, namely organoleptic tests, homogeneity tests, dispersibility tests, and pH tests. This research was conducted by the smelting method. The results showed that the three creams of ethanol extract of rambusa leaf (Passiflora foetida L) had met the requirements for physical properties of homogeneity, spreadability, and pH.

Evaluation of inhibitory and probiotic properties of lactic acid bacteria isolated from vaginal microflora.

Lactic acid bacteria (LABs) are known to secrete species-specific secondary metabolites that could be utilized as novel therapeutics against multi-drug resistant pathogens. This study aimed to investigate the antagonistic and probiotic properties of LABs isolated from the vaginal ecosystem of healthy women and to assess the stability of their antagonistic metabolites. Among 43 strains isolated from healthy women, eight LAB strains exhibited detectable BLISs (bacteriocin-like substances) producing ability against E. faecalis (JH-86), S. aureus (JH-68), Streptococcus sp. (JH-80), and E. coli (JH-101), with zone of inhibition (ZI) ranging from 9.00 to 20.33mm and minimum inhibitory concentrations (MICs) from 62.5 to 500 μL/mL, respectively. The partially purified compounds extracted from cell free supernatant (CFS) displayed an increase in antagonistic activity based on ZI, 9.67-30.17mm and MICs, 3.91-15.63mg/mL, respectively. In a time-kill study, both crude and partially purified compounds of Limosilactobacillus reuteri (MT180537), Pediococcus pentosaceus (MT176555), Limosilactobacillus pontis (MW362838), and Levilactobacillus brevis (MW362790) exhibited significant bactericidal action against E. faecalis (MW051601), the most frequent etiological agent of aerobic vaginitis (AV). The active secondary metabolites from L. reuteri (MT180537), P. pentosaceus (MT176555), and L. pontis (MW362838) were protein in nature and remained stable under different physicochemical conditions. Regarding probiotic properties, the strains presented probiotic characteristics, i.e., good acid, bile salt tolerance, aggregation properties, and biofilm formation. The strains were susceptible to most of the commonly used antibiotics and had no hemolytic activity. In conclusion, antagonistic compounds or BLIS produced by L. reuteri (MT180537) could be investigated further for preparation of ointments to treat AV.

Formulasi Sediaan Salep Ekstrak Minyak Atsiri Daun Jeruk Purut (Citrus Hystrix DC.) terhadap Jamur Candida Albicans

&lt;p&gt;&lt;em&gt;T&lt;/em&gt;&lt;em&gt;he purpose of this&lt;/em&gt;&lt;em&gt; study &lt;/em&gt;&lt;em&gt;was&lt;/em&gt;&lt;em&gt; to formulate essential oils in ointment preparations with different bases and to determine the physical properties and antifungal activity against Candida albicans.&lt;/em&gt;&lt;em&gt; &lt;/em&gt;&lt;em&gt;The essential oil of kaffir lime leaves was obtained by distillation using the water distillation method and the raw material of fresh kaffir lime leaves was 1.5 k&lt;/em&gt;&lt;em&gt;ilograms&lt;/em&gt;&lt;em&gt; which was then identified with Sudan III reagent and formulated in ointment preparations.&lt;/em&gt;&lt;em&gt; &lt;/em&gt;&lt;em&gt;The ointment is made with FI, FII, and FIII base formulations with an active substance concentration of 7%. The FIII ointment base had the best evaluation results and was continued for antifungal testing against Candida albicans.&lt;/em&gt;&lt;em&gt; &lt;/em&gt;&lt;em&gt;From the research results obtained % yield of essential oil of kaffir lime leaves is 1.533%. The results of the phytochemical identification test showed positive results with the formation of an even red color in the solution.&lt;/em&gt;&lt;em&gt; &lt;/em&gt;&lt;em&gt;The results of the evaluation of the ointment preparations showed that FIII had the best evaluation results and continued antifungal testing with a positive control of 2% miconazole nitrate cream.&lt;/em&gt;&lt;em&gt; &lt;/em&gt;&lt;em&gt;The average diameter obtained is 8.43 mm and is included in the medium category.&lt;/em&gt;&lt;em&gt; &lt;/em&gt;&lt;em&gt;The results of the Shapiro Wilk normality test showed that the research data was normally distributed and homogeneous, then the One-Way ANOVA test was carried out and a significance value of P&amp;lt;0.05 was obtained, and the LSD test showed a significant difference seen from the significance value obtained P&amp;lt;0.05.&lt;/em&gt;&lt;em&gt;&lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;em&gt; &lt;/em&gt;&lt;/p&gt;

Formulation preparation and evaluation of Apigenin ointment for anti-inflammatory and wound healing activities

Formulation preparation and evaluation of Apigenin ointment for anti-inflammatory and wound healing activities

Implications of a Diabetic Foot Xerosis Treatment With an Emulsion Containing the Plant-Based Anionic Phospholipids.

Purpose:This study compares and contrasts a skin cream containing plant-based anionic polar phospholipid (APP) technology with a mineral oil hydrocarbon (petrolatum)-based (MHB) skin cream technology in the treatment of skin xerosis (dryness) in diabetic feet. Skin cream with APP technology promotes phospholipid absorption, reparation of intercellular lamellae, and organization of water promoting hydration; whereas skin cream with mineral hydrocarbon-based (MHB) technology principally covers skin, preventing dehydration.Methods:Subjects (n = 54) with diagnoses of diabetes mellitus and foot skin dryness were studied using a multicenter, double-blind, masked-study design. An emulsion cream containing 0.05% APP in triglycerides (APP preparation) was compared to MHB skin cream, Eucerin® (MHB preparation) applied topically to skin of the feet. Graded measurements were recorded on 4 efficacy variables including dryness, erythema, fissures, and itching and neurovascular assessments. Implications of the plant-based and mineral-based skin creams in the context of skin xerosis are contrasted.Results:APP and MHB preparations were similar in effectiveness and safety. There was no significant difference among any of the 4 efficacy variables (P < .5) including neurovascular measurements. The APP preparation is absorbed into the skin, whereas the MHB skin cream leaves detectable residues after each application.Conclusion:Although the APP and MHB preparations were not significantly different in effectiveness and safety, distinctively, application of the APP skin cream preparation absorbed into the skin leaving no discernible residue in contrast to the MHB preparation leaving residues potentiating textile damage. Both of these technologies function in the hydration of skin; however, they differ in their modes of action. The plant-based APP preparation functions actively by phospholipid and triglyceride absorption, reparation of skin lamellae, and in the consequent delivery and organization of waters of hydration in skin. The MHB preparation functions passively, hydrating the skin it covers by sealing the skin against dehydration.

Formulation of Black Mulberry Fruit (Morus nigra l.) Ethanol Extract as Anti-Acne Cream Against Propionibacterium acne

Various parts of mulberry have been identified consisting of a large number of phenols and flavonoids, which are effective for immunosuppression, antioxidants, and are antibacterial, and anticancer agents. A polysaccharide and an alkaloid related to increased macrophage activity from mulberry fruit extracts. This study aims to formulate black mulberry fruit into cream dosage form and determine the antibacterial activity against Propionibacterium acnes. This is an experimental research which varying extract into 4 different concentrations: 6.25%, 12.5%, 25%, and 50%. There is two control group which is positive using vitacid cream and the negative control is cream base without extract. The cream was made and examined for its physical characteristic and the cream was tested for antibacterial activity using the Kirby-Bauer disk diffusion method. The result showed that the cream met the requirements of homogeneity, pH as its requirement, stable during storage, not irritating, and the antibacterial activity of 6,25% concentration has an average inhibition zone diameter of 10.15mm, a concentration of 12,5% is 12.36mm, a concentration of 25% is 13.16mm, and a concentration of 50% is 15.5 mm. From this study can be concluded that the cream preparation from an ethanol extract of black mulberry has good antibacterial activity against P.acne bacteria with a very strong inhibition zone.

Effectiveness of Turmeric Ethanol Extract Cream Preparation (Curcuma Longa) in Speeding up Wound Healing in Male Wistar Rats

Injury is the loss or damage of some body tissues, due to a factor that interferes with the body's protective system. The results of several studies state turmeric rhizome extract is effective in wound healing. This study aims to find out the effect of turmeric extract (Curcuma longa) on wound healing in white rats. This type of research is experimental with the approach of Pre-test and Post-test group only control design, conducted November to December 2020. The samples used were turmeric rhizomes (Curcuma Longa) and male white rats. Determination of the size of the sample according to Federer's formula, so that the number of samples as many as 25 mice, the division of 4 treatment groups and 1 control group. The results of the study from the Anova test obtained a Sig value. Calculate 0.000 while sig value (α) is 0.05 which means sig value. Count sig &lt; (α). This means that there is a real influence of the administration of turmeric extract (Curcuma Longa) on the healing of sores in mice. Turmeric ethanol extract (Curcuma Longa) has several bioactive compounds such as alkaloids, flavonoids, saponins, and tannins that play a role in wound healing. The optimum concentration of turmeric ethanol extract (Curcuma Longa) that can cure sores in white rats is 5%. The highest percentage of cures on k-14 days was in the positive control (Bioplacenton®) which was 95% and followed by extract 5% v/ v with a cure percentage of 90%. The creamy preparation of turmeric ethanol extract (Curcuma Longa) has an ability that is close to Bioplacenton® in healing wounds in mice.

Characteristic Evaluation of Various Formulations of Anti-Aging Cream from Carotenoid Extract of Bacterial Symbiont Virgibacillus salarius Strain 19.PP.Sc1.6

Premature aging can be triggered by free radicals from UV rays, since exposure to these rays can cause the skin to experience oxidative stress. Oxidative stress induces intracellular DNA damage, protein denaturation, and lipid peroxidation that lead to cell death. However, cell death can be prevented with antioxidants such as carotenoids, which are among the potential natural compounds for its treatment. Sources of carotenoids include microbial symbionts associated with Sinularia sp., one of which is the bacterium Virgibacillus salarius strain 19.PP.Sc1.6, a carotenoid-producing bacteria. This study aims to explore the utilization of carotenoids from the bacterium V. salarius strain 19.PP.Sc1.6 for the preparation of anti-aging creams. Furthermore, the method employed three formulations (vs, ow, and wo) containing different types of cream tested for stability, and antioxidant and sunscreen abilities. The results obtained established that the carotenoid extract from V. salarius strain 19.PP.Sc1.6 was more stable in the cream vs. the oil-in-water type cream with an anionic emulsifier.

Effect Collagen Concentration Tilapia Scales on the Quality of Burn Ointment

Collagen is one of the main connective tissue animal proteins and has been widely used as a biomedical material. Collagen is divided into XIX types. Type I collagen, among others, is obtained from bone, scales and skin. Collagen derived from type I can repair tissue or accelerate tissue regeneration to heal burns. The purpose of this research was to determine the addition of fish scale collagen extract to the characteristics of the burn ointment preparation in accordance with the Indonesian National Standard (SNI) and the best quality. The method used in this research is an experimental method of Completely Randomized Design (CRD) consisting of 4 collagen addition treatments: 0%, 2%, 4% and 6% repeated 5 times. Parameters in this method include physical-chemical parameters (pH, spreadability, shelf life and homogeneity) and organoleptic parameters (appearance, aroma, texture and color). Bayes test results, the concentration of the addition of tilapia scale collagen in the ointment preparation of 4% resulted in a value close to the control treatment. The addition of 4% collagen was the best treatment compared to 2% and 6% with a pH value of 6.12, dispersion of 3.22 cm, safe ointment preparation did not change at all during 28 days of storage. Based on the results of the organoleptic test parameters, the ointment at this concentration had a homogeneous appearance, slightly yellowish white color, a distinctive smell of collagen and a semi-solid texture, this was in accordance with the quality standard of the ointment and had the best quality characteristics.