Cream Preparations Research Articles

“Rede hit sofft”: John Audelay's Practice of Care

“Rede hit sofft”: John Audelay's Practice of Care

Low-fat ice cream model system: impact of incorporation of alcalase hydrolyzed zein.

Research on fat substitutes with low calories and good flavor is important to reduce the fat content in food. In this paper, the selection of fat substitutes and the preparation of low-fat ice cream were carried out through looking at the emulsion properties of the enzymatic hydrolysis of zein. The results showed that the emulsifying activity of zein after enzymatic hydrolysis for 10 min was 66.76 m2 g-1, and the emulsifying stability was 78.51 min, showing the best emulsifying properties. Enzymatic hydrolysis of zein can effectively reduce the degree of lipid oxidation. The protein digestibility in intestinal juice was also significantly improved, and the release rate of free fatty acids in the emulsion reached more than 80%. The viscosity, shear stress, elastic modulus, electronic nose and electronic tongue of ice cream with 10% oil substitute were close to those of full-fat ice cream. It is expected to provide a basis for the development of functional foods.

Preparation and Evaluation of Flaxseed Oil Cream

Herbal cosmetics are the beauty products, which posses desirable physiological properties like Skin healing, smoothening, appearance, enhancing and conditioning properties due to the herbal ingredients. Cosmetics are safe, effective, elegant properties. Whatever may be the type of skin, these three steps acts as External care for skin to protect it from the constant effect of environment, stress and skins natural process of cell degradation decay. Flaxseed oil cream are used for heal cracked fingertips during winter; whenever You apply cream to your face, hand, leg, cover the cracked area with a nice thick layer too, to seal in moisture and protect the area.

Formulation of Hand Cream Essential Oil of Basil (Ocimum basilicum) Leaves

Basil (Ocimum basilicum) leaf essential oil contains terpenoid compounds that have antibacterialactivity. This study aims to obtain hand cream preparations of basil essential oil, as well as to determinethe effect of concentration variation of liquid paraffin on the results of the evaluation of the preparation.In this study, three formulations of hand cream preparations of basil essential oil were made withthree variations in the concentration of liquid paraffin, namely F1 (9%), F2 (10%), and F3 (11%).Furthermore, the preparation was evaluated for 28 days at room temperature (15-300C) and humidity(60-80%) which included organoleptic, pH, viscosity, homogeneity, spreadability, and type of emulsion.The results showed that formulas F1, F2, and F3 met all the requirements of organoleptic, pH, viscosity,homogeneity, spreadability, and type of emulsion. The results of the statistical analysis showed thatvariations in liquid paraffin concentrations had a significant effect (p<0.05) on the evaluation ofviscosity and did not significantly affect the evaluation of organoleptic, evaluation of pH, evaluation ofhomogeneity, evaluation of spreadability, and evaluation of emulsion type.

Anti Acne Cream Formulation Ethanol Extract of Katuk Leaves (Sauropus androgynus (L.) Merr against Staphylococcus Epidermis and Staphylococcus Aureus

Acne is a skin problem that generally occurs during puberty. Under normal conditions, during puberty, there are Staphylococcus epidermal bacteria and Propionibacterium acnes that proliferate so that inflammation occurs, causing acne on the skin. Since ancient times, our ancestors believed in the use of natural ingredients to treat various diseases, one of which was katuk leaves. Katuk plants have secondary metabolic compounds such as alkaloids, saponins, flavonoids and tannins. The potential of Katuk leaves has been tested for many pharmacological activities, such as antibacterial, antihypertensive, anti-infective, antihyperlipidemic, antianemic and also increases milk production. Katuk leaves are often used in the form of extracts as antibacterial, so it needs to be developed into a pharmaceutical preparation to increase its use. One of the pharmaceutical preparations is cream preparations to treat the causes of acne. This study aims to determine the ability of katuk leaf extract cream to act as an antibacterial against Staphylococcus Epidermis and Staphylococcus Aureus bacteria.. The design of this study is an experimental method, making katuk leaf extract by maceration method by immersing in 70% ethanol solution for 5 days. After 5 days, the pulp and filtrate were separated and then evaporated using a Vacuum Rotary Evaporator to obtain a thick extract. The preparation of katuk leaf extract cream was carried out with a concentration of 10%, 15%, 20% and an antibacterial test was carried out using the pitting method. The antibacterial test results of the katuk leaf cream extract showed an inhibition zone marked with a clear area around the well. Katuk leaf extract cream was able to inhibit the growth of S. aureus and S. epidermis bacteria with the best concentration in formula 3, namely the concentration of katuk leaf extract of 20%.

The Effect of Xylocarpus granatum J. Koenig Seed Extract Cream on The Number of Fibroblast and Re-Epithelialization in IIA Degree Burn Wound Healing

Burns require a recovery process that multiplies complex biological mechanisms including anti-inflammatory, proliferation, and tissue remodeling. X.granatum J.Koenig (XG) is a popular plant that grows in the mangrove forest Bali. Traditionally, this plant is used mainly for antiinflammation, dysentery, fever, skin emolliating, wound treatment, and abdominal problems. Flavonoids, tannins and saponins are active metabolites of this plant, which has antiinflammation, antioxidant and wound healing effects. IL-1β, IL-6 and TNF-α are pro-inflammatory cytokines that are involved in the pathophysiology of wound. Fibroblasts support granulation tissue synthesis, ECM production, and collagen synthesis. Re-epithelialization plays a role in wound closure. The aim of present study to evaluate the effectiveness XG extract seeds cream on burn wound grade IIA in mice. XG extract was evaluated for antioxidant activity by DPPH method. The two cream formulations were measured for physical quality for organoleptic, homogeneity, and pH. The wound healing activity of XG creams were evaluated from wound contraction, histological analysis of fibroblast proliferation and re-epithelialization in mice. Statistical analyses were performed with post hoc Tukey using analysis of variance and were analyzed using Statistical Package for the Social Science (SPSS, Version 26). XG extract has a very strong antioxidant activity with an IC50 value of 7,939 ppm. The preparation of XG extract cream has homogeneous and pH of 6,5. The treatment with XGC 15% promote significant increases in wound contraction rate, proliferation of fibroblasts and re-epithelialization with SSD (p>0,05). These results suggest that XGC 15% is effective in wound healing in mice by increasing wound contraction rate, proliferation of fibroblasts and re-epithelialization.

Formulation and Evaluation of Body Scrub Cream Containing Ethanol Extract of Black Cumin Seeds (Nigella sativa L.) and Coffee Grounds (Coffea arabica L.) as Anti-aging

Cosmetics are now mandatory for most women. Various natural ingredients have now been used as basic ingredients for beauty treatments. This study aimed to formulate and determine the effectiveness of body scrub cream preparations containing ethanolic extract of N. sativa and coffee grounds as antiaging. The skin antiaging was divided into five formulas: F0 (cream base), F1 (coffee ground 5%), F2 [coffee ground 5% + Ethanol Extract of N. sativa (EENS) 2%], F3 (coffee ground 5% + EENS 3.5%) and F4 (coffee ground 5% + EENS 5%). The body scrub cream preparations were subjected to organoleptic, homogeneity, emulsion type, and pH evaluation. The irritation test and antiaging activity were evaluated by using a skin analyzer. The body scrub was stable in 12-week storage. The result showed that there was no irritation on the volunteers’ skin. The body scrub cream with the highest antiaging activity was the F4 formula. It showed 9.6% for increasing skin moisture, 17.68% for increasing skin smoothness, 11.42% in reducing the size of pores and 12.84% in reducing the number of spots.

Sunscreen Cream Formulation of Noni Leaf Extract (Morinda citrifolia L.) with Emulsifier Combination of Tween 80 and Lecithin

Background: Noni leaf (Morinda citrifolia L.) extract is a natural product that can be used as a sunscreen. The extract contains flavonoids which function act as an antioxidant. In this work, sunscreen cream formulated with noni leaf extract was prepared using a combination of tween 80 and lecithin. Objective: The purpose of this study is to evaluate how the combination of tween 80 and lecithin affects the physical qualities of the cream, such as organoleptic, homogeneity, emulsion type, spreadability, adhesion, pH, and stability over 28 days of storage at room temperature, and to find the best formula. Methods: This study used 10% of noni leaf extract in the cream formulation. The Simplex Lattice Design (SLD) method was used to determine the effect of different concentrations of the two emulsifiers on the cream's spreadability, adhesion, and pH. Furthermore, the SLD was used to find the best formula. Results: The results showed that different concentrations of the emulsifier, which are the tween 80 and lecithin combination, affected the physical properties and storage stability of cream preparations. The interaction of tween 80 and lecithin is having a significant impact on the cream's adhesion and spreadability; however, the effect of the interaction on the pH value was not significant. Conclusion: The formula containing 2.5 % tween 80 and 2.5 percent lecithin was found to be the most effective in fulfilling the cream physical properties while remaining stable during storage.

Effectiveness Test of White Skin Extract Cream from Red Watermelon (Citrullus Lanatus) on Increasing Elasticity, Sebum and Hydration in White Mice (Mus Musculus) Skin

Skin moisturizing gel can provide a moisturizing effect on the skin. One of the natural ingredients that can be made into gel preparations is the white peel of watermelon (Citrullus Lanatus) because it contains a lot of citrulline, vitamin A, vitamin B2, vitamin B6, vitamin E, vitamin C, and protein, beta-carotene, lycopene, and water. The purpose of this study was to determine the activity of the ethanol extract of watermelon rind on increasing elasticity, sebum, and hydration in the skin of male white mice. This study used the Pretest-Posttest Control Group Design method. The stages of the research were sample collection, extraction, formulation of various concentrations of moisturizing gel preparations (0, 2.5%, 5%, and 10%). Tests were carried out on the skin of male mice for 4 weeks by measuring levels of elasticity, sebum, and hydration. The results of the phytochemical screening extracts contained flavonoids, saponins, steroids, triterpenoids, and glycosides. The evaluation results showed that all formulas met good preparation parameters and did not irritate the skin. The measurement results in the fourth week of each cream preparation group showed an increase in the levels of elasticity, sebum and hydration and differed significantly from the blank group (p <0.05) and the highest increase occurred in cream with watermelon rind extract concentration 10 %. The results of this study indicate that the cream preparation of ethanol extract of watermelon rind can increase the levels of elasticity, sebum, and hydration of the skin.

Test Of the Effectiveness of Tomato (Lycopersicum Esculentum Mill) Fruit Extract Cream on Increasing Elasticity, Sebum and Hydration in White Mouse Skin (Mus Musculus)

The skin is the outermost organ that acts as a protector from the external environment both in the ligaments, muscles, and internal organs from exposure to direct sunlight (ultraviolet light) and dehydration, also functions as a sense of taste and touch as well as a defense against pressure and infection. The imbalance between oxidants and antioxidants is influenced by external factors and internal factors that will affect wrinkling and aging of the skin. Tomatoes contain substances such as citrulline, vitamin A, vitamin B2, vitamin B6, vitamin E, vitamin C, and protein, beta-carotene, lycopene, and water. The purpose of this study was to determine the activity of tomato fruit ethanol extract to increase elasticity, sebum and hydration in the skin of male white mice. Cream preparations of tomato fruit ethanol extract were formulated with concentrations of 2.5%, 5%, and 10%. The test was carried out on the skin of male mice for 4 weeks by measuring the levels of elasticity, sebum and hydration. The results of phytochemical screening of extracts contained flavonoids, saponins, steroids, triterpenoids, and glycosides. The measurement results in the fourth week of each group of cream preparations showed an increase in the levels of elasticity, sebum and hydration and was significantly different from the blank group (p<0.05) and the highest increase occurred in cream with 10% tomato extract concentration. The results of this study indicate that the preparation of tomato fruit ethanol extract cream can increase the elasticity, sebum and hydration levels of the skin.

Formulasi dan Uji Stabilitas Fisik Sediaan Lulur Krim Ekstrak Daun Pandan Wangi (Pandanus amaryllifolius Roxb.)

Scrub cream is a pharmaceutical preparation which function is to remove dead skin cells, moisturize and brighten the skin with the help of scrub ingredients. One of the natural ingredients used as raw material for scrub cream is fragrant pandan (Pandanus amaryllifolius Roxb.). The aim of this study was to make a cream scrub preparation from pandan extract with various concentrations of 3%, 3.5% and 4%. This research was conducted experimentally. Preparation testing includes organoleptic test, homogeneity test, pH test, irritation test, spreadability test, stability test, and emulsion type test. The results of the physical quality inspection of the scrub cream from pandan Wangi extract showed that the preparation was homogeneous, had an M/A emulsion type, had a pH value of 6.1-6.5, did not cause irritation and did not change during storage for 4 weeks except for the preparation with a concentration of 3.5% because the preparation underwent a change in the breakdown of the emulsion in the preparation on storage starting from the 3rd week. From the research that has been done, it can be concluded that Pandanus amaryllifolius Roxb extract can be formulated as a scrub cream with a variation of concentration of 3%; 3.5% and 4% and does not irritate the volunteers' skin.

Evaluation of Body Scrub from Boergesenia forbesii and Kaempferia galanga

The use of cosmetics at this time prioritizes natural ingredients over synthetic materials. The materials that are often used and become potential are green seaweed and rhizomes. Boergesenia forbesii and Kaempferia galanga are two natural ingredients with bioactive content that is good for human skin. The combination of these two natural materials has never been studied. This study aims to evaluate the best formulation of body scrub preparations from these two natural ingredients. In this study, the ratio of B. forbesii and K. galanga was 1:1 (cream R1), 1:2 (cream R2), 2:1 (cream R3), and control (no added natural ingredients) (cream R0). The cream preparations were evaluated by viscosity, emulsion type, stability (emulsion), skin moisture, and irritation test. Viscosity values in cream preparations ranged from 22,500 cPs to 34,600 cPs. All the treatment belongs to the type of water soluble oil (O/W). Cream R3 was the best for the stability of emulsion (88.89%). The skin moisture parameter for R0, R1, R2, and R3 were 40.44%, 52.75%, 54.34%, and 57.55%, respectively. Based on the results of the irritation test on the skin (for all treatments), there were no volunteers who experienced signs of irritation such as rash, itching, and edema. So, the best formulation was cream R3, based on the evaluation of all parameters.

Enhancing effects of chemical permeation enhancer propylene glycol and ultrasonic technology on transdermal permeation of Baimai Ointment

This study aimed to improve the transdermal permeation quantity of Baimai Ointment by investigating the enhancing effects of physical and chemical permeation promoting methods on transdermal permeation of Baimai Ointment. The improved Franz diffusion cell method was used for in vitro transdermal experiment. The abdominal skin of mice was used, and the skin was treated with 3% propylene glycol in the chemical enhancement group. Ultrasonic technology was introduced in the physical enhancement group. The conditions of ultrasonic technology were optimized by single factor trial. Taking Q_(EF) and ER as the indexes of penetration promotion performance, the enhancing effects of the two methods were compared. The results showed that the promotion performance of 3% propylene glycol for ammonium glycyrrhizinate, nardosinone and curcumin of the chemical enhancement group were 1.74, 1.60, and 3.73 times higher than those of the blank group, respectively. The overall permeation efficiency of the Baimai Ointment was significantly improved. The comprehensive promoting effect on each component was curcumin>ammonium glycyrrhizinate>nardosinone. In the physical enhancement group, the penetration promoting effect of ultrasonic power 1.0 W was better than that of 2.0 W and 0.5 W, ultrasonic time 5 min was better than 3 min and 8 min, and the ultrasonic frequency 1 MHz was better than 3 MHz. Therefore, the optimal ultrasonic condition was 1.0 W-5 min-1 MHz. Under this condition, in terms of the transdermal permeation for ammonium glycyrrhizinate, the Q_(EF) and ER of the ultrasonic technology were better than those of 3% propylene glycol. In terms of the transdermal permeation for nardosinone and curcumin, the QEF and ER of 3% propylene glycol were better than those of the ultrasonic technology. Therefore, 3% propylene glycol combined with ultrasonic technology can be used to promote permeation of Baimai Ointment that contains both water-soluble and fat-soluble components in the clinical application. This study provides a theoretical basis for the clinical application of Baimai Ointment and other transdermal preparations.

Pemberdayaan PRA Tamantirto Utara melalui Pelatihan Pembuatan Es Krim Jamu dan Sosialisasi DAGUSIBU serta Pemeriksaan Kesehatan

Pimpinan Ranting Aisyiyah (PRA) Tamantirto Utara is part of the Muhammadiyah association. Universitas Muhammadiyah Yogyakarta (UMY), especially the Pharmacist Professional Education Study Program has the responsibility to carry out the guidance and empowerment of PRA. The purpose of this community service activity is to empower PRA Tamantirto Utara in the introduction of health products in the form of training in making herbal ice cream and improving health status through DAGUSIBU (Get, Use, Save and Dispose of) medicine and health checks. This activity is in collaboration with the student hermitage of the UMY Pharmacist Professional Education Study Program. The method used in this activity is through training in making herbal ice cream, DAGUSIBU counseling, health checks (cholesterol checks, blood sugar and uric acid) which ends with the provision of equipment grants. The activity begins with a pretest and ends with a posttest to evaluate the participants' understanding. The management of the PRA Tamantirto Utara received knowledge of herbal medicine processing into ice cream preparations, as well as a comprehensive understanding of DAGUSIBU as evidenced by an increase in the pretest score of 88.57% to 97.62%. The results of the examination showed that only about 19.04% had hypercholesterolemia, 9.52% had hyperglycemia, and 14.28% had hyperuricemia. Education on the handling of metabolic disorders was given to the PRA Tamantirto Utara. This community service activity provides skills and insight into making herbal ice cream, understanding DAGUSIBU and efforts to improve health status for PRA Tamantirto Utara.

Aktivitas Antioksidan dan Evaluasi Fisik Sediaan Body Scrub dari Bubur Rumput Laut Boergesenia forbesii dan Serbuk Kencur (Kaempferia galanga)

Seaweed has bioactive components that are very much needed in cosmetic preparations, in addition to seaweed, natural ingredients that can be used are kencur (Kaempferia galanga). This study aims to determine the best combination of B. forbesii seaweed porridge and kencur (K. galanga) powder as a body scrub preparation from the results of antioxidant activity and physical evaluation. This research was conducted by looking at the difference in concentration between B. forbesii and kencur (K. galanga) seaweed with a ratio of 1:1 (R1 cream), 1:2 (R2 cream), and 2:1 (R3 cream) and without the addition of seaweed. and Moringa (R0). The cream preparation was then tested for antioxidants and physical evaluation of the preparation included pH, homogeneity, centrifugal test, and organoleptic observations. The test results showed that all formulations of body scrub cream which were given B. forbesii seaweed porridge and kencur (K. galanga) powder had weak antioxidant activity. The results of the evaluation of physical preparations showed good results for the skin, but the formulation of R2 cream which is the best cream seen from the overall evaluation results

Test the Potential of Macadamia Nut Oil (Macadamia F. Muell) As Sunscreen in Cream Preparations In Vitro

Background: Sunlight has a beneficial effect by activating provitamin D3 found in the epidermis of the skin to become vitamin D3. However, excessive sun exposure can also have detrimental effects, especially on the skin. Macadamia nut oil contains vitamin E (tocotrienols and tocopherols) which are natural antioxidants. Macadamia nut oil also contains linoleic acid which is great for softening the skin, regenerating skin cells, moisturizing the skin, and is a natural anti-inflammatory. and helps restore skin barrier function and reduce transepidermal water loss (TEWL). Objectives: To determine whether there is a sunscreen potential from macadamia nut oil, the difference in the SPF value is significant between cream preparations with the addition of macadamia nut oil in various concentrations and to determine the sunscreen category of each macadamia nut oil formulation. Methods: This research was carried out experimentally, including the preparation of macadamia nut oil lotion, pH test, irritation test, and in vitro determination of the SPF (sun protection factor) number using a UV-Vis spectrophotometer. Results: The addition of macadamia nut oil combined with oxybenzone and octylmetoxycinamate in cream preparations has an average SPF value of F1 (blank) of 9.97; F2 (blank + 2% oxybenzone + 5% octyl methoxycinamate) of 23.14; F3 (1% macadamia nut oil) of 29.39; F4 (5% macadamia nut oil) of 32.99; and F5 (10% macadamia nut oil) of 37.59. The addition of macadamia nut oil in various concentrations gave a significant difference in increasing the SPF value between each formula combined with oxybenzone and octylmetoxycinamate. Conclusion: The addition of macadamia nut oil can increase the effectiveness of the combination oxybenzone and octylmetoxycinamate sunscreen in cream preparations.

Release of salicylic acid from new multiphase bases with consideration of the preparation method

Abstract: The study evaluated the release of salicylic acid with new two multiphase ointment bases: Fitalite™ and Nourivan™ Antiox. Fitalite™ is a cream-gel base while Nourivan™ Antiox is a cream oil in water. Salicylic acid is a substance often used in the formulation of semi-solid dosage forms applied on the skin. The release rate of salicylic acid was examined depending on the base and the particle size of the active ingredient. For this purpose, formulations containing 5% salicylic acid were prepared and used for the study. Three different methods were used for the incorporation of salicylic acid into the bases: non-grinded, ground with the base for 15 minutes, and concentrate of salicylic acid with white soft paraffin (1:1). Measurements of the particles of the active substance in each of the preparations were also taken. Salicylic acid has been shown to be released to a greater extent from Fitalite ™ base. Qualitative analysis of the composition of the bases, in studies conducted by the Department staff, allows concluding that the amount and release rate of salicylic acid from the bases is influenced by its viscosity. This parameter has a significant impact on the level of trituration of salicylic acid. It was also found that the concentrate of salicylic acid with white soft paraffin (1:1) reduces the time of preparation of a suspension type ointment with salicylic acid, wherein the particle size accomplishes the requirements of pharmacopeia.

Pemanfaatan Pliek U (Bumbu Khas Aceh) sebagai Krim Antibakteri

This study aims to determine the antibacterial activity of Pliek U ethanol extract cream against Staphylococcus aureus. The method was an experimental method with a completely randomized design (CRD) on five treatments, namely positive control, negative control, F1, F2, and F3, each with four repetitions. Antibacterial testing was carried out using the disc diffusion method. The results showed that the average diameters of F1, F2 and F3 were 12.65 mm, 14.9 mm and 12.92 mm, which were included in the solid antibacterial category in inhibiting the activity of Staphylococcus aureus bacteria. Pliek U can be formulated as a cream preparation, meets the requirements for quality tests for cream preparations and has met SNI standard parameters. In conclusion, Pliek U ethanol extract cream has antibacterial activity with the most optimum effectiveness at a concentration of 5%. 
 Keywords: Antibacterial, Cream, Pliek U, Staphylococcus aureus

Comparative Analysis of Salmonella enterica subsp. enterica Serovar Thompson Isolates associated with Outbreaks Using PFGE and wgMLST

The strains associated with foodborne Salmonella enterica Thompson outbreaks in Korea have not been identified. Therefore, we characterized S. Thompson strains isolated from chocolate cakes linked to foodborne outbreaks in Korea. A total of 56 strains were isolated from preserved cake products, products in the supply chain distribution, the manufacturer's apparatus, and egg white liquid products used for cream preparation. Subsequently, serological typing, pathogenic gene-targeted polymerase chain reaction (PCR), pulsed-field gel electrophoresis (PFGE), and whole-genome multi-locus sequence typing (wgMLST) were performed to characterize these isolates. The antigen formula of all isolates was 7:k:1,5, namely Salmonella enterica subsp. enterica Serovar Thompson. All 56 isolates harbored invA, his, hin, and stn, and were negative for sefA and spvC based on gene-targeted PCR analyses. Based on PFGE results, these isolates were classified into one group based on the same SP6X01.011 pattern with 100% similarity. We selected 19 strains based on the region and sample type, which were subjected to wgMLST. Although the examined strains showed 100% similarity, they were classified into seven clusters based on allelic differences. According to our findings, the cause of these outbreaks was chocolate cake manufactured with egg white liquid contaminated with the same Salmonella Thompson. Additionally, comparative analysis of wgMLST on domestic isolates of S. Thompson from the three outbreaks showed genetic similarities of over 99.6%. Based on the results, the PFGE and wgMLST combination can provide highly resolved phylogeny and reliable evidence during Salmonella outbreak investigations.

Pengaruh Penambahan Enhancer Isopropil Miristat dan dan Propilen Glikol terhadap Penetrasi Krim Asiklovir

Acyclovir, a BCS class III drug, has a high ability to fight the herpes virus but has low permeability and is poorly soluble in water. In order to obtain the expected effect of viral infection on the skin, the penetration of the active ingredients becomes a critical factor for a topical treatment to achieve the desired concentration. This study was to see the effect of adding isopropyl myristate and propylene glycol as enhancers that help diffuse the drug through the stratum corneum. The formulation is made by adding enhancer propylene glycol 8% (F1), isopropyl myristate 8% (F2), and without enhancers (F3). Physical stability of acyclovir cream, including homogeneity, organoleptic, pH, viscosity, type of emulsion and power spread, and in vitro cream penetration test with Franz diffusion cells will be observed. The result of acyclovir cream preparations F1, F2, and F3 had a homogeneous preparation of white semisolid and odorless with pH between 7,30-7,60, emulsion type o/w, and a viscosity between 35000-47000 cPS. The cumulative amount of acyclovir penetrated at F3 was 226,008± 8,462 μg/cm2, while the cream containing the enhancers F1 258,059±1,730 μg/cm2 and F2 268,336±0,740 μg/cm2, respectively. The presence of enhancers will increase the penetration ability of acyclovir cream, with more penetration using isopropyl myristate than propylene glycol.