Matrix Solid-phase Dispersion Research Articles

Rapid and Efficient Isolation of Anti-Inflammatory Components From <i>Lindera aggregata</i> (Sims) Kosterm Leaves by Combining Matrix Solid Phase Dispersion Extraction With High-Speed Countercurrent Chromatography

Rapid and Efficient Isolation of Anti-Inflammatory Components From <i>Lindera aggregata</i> (Sims) Kosterm Leaves by Combining Matrix Solid Phase Dispersion Extraction With High-Speed Countercurrent Chromatography

Migration studies of chloropropanols from paper straws: an improved method using GC-MS/MS

ABSTRACT An optimised method was established for the determination of four chloropropanols in paper straws, namely 1,3-dichloropropan-2-ol(1,3-DCP), 2,3-dichloropropan-1-ol (2,3-DCP), 3-monochloropropane-1,2-diol (3-MCPD) and 2-monochloropropane-1,3-diol (2-MCPD). After extraction with a food simulant, purification with matrix solid phase dispersion (MSPD), and derivatisation using heptafluorobutyrylimidazole (HFBI), the chloropropanols were analysed by gas chromatography–tandem mass spectrometry (GC-MS/MS), and quantified with a deuterated internal standard. Under the optimised conditions, the limit of detection (LOD) was 0.200 µg/L for the four target compounds. The recoveries were in the range of 71.4% – 119.4% with relative standard deviations (RSDs) of 0.4% – 9.9%. The method fulfilled the requirements of the European regulations for the quantification of 3-MCPD and 1,3-DCP. Fifteen paperboard straws were tested using the proposed method. The results showed that 1,3-DCP and 3-MCPD exhibited the highest detection rate among the four chloropropanols tested (93.3%). the method can be used to detect chloropropanol contamination in paperboard products, and further to guide food manufacturers to reduce the levels of chloropropanols in paperboard food contact materials.

A Simple Method for the Determination of Pharmaceutical and Personal Care Products in Fish Tissue Based on Matrix Solid-Phase Dispersion.

A simple and effective pretreatment method based on matrix solid-phase dispersion was developed for the determination of pharmaceutical and personal care products (PPCPs) and their metabolites in fish by high-performance liquid chromatography tandem mass spectrometry. The type and amount of dispersant, adsorbent, and eluting solvent were optimized by a single-factor experiment and Box-Behnken design. Under the optimal conditions with 2.5 g of Florisil as a dispersant, 500 mg of C18 as an adsorbent, and 5 mL of acetonitrile as an eluting solvent, the recoveries ranged from 70.4 to 99.9% with relative standard deviations less than 10.5%, and the limits of quantitation ranged from 0.13 to 1.01 μg/kg. The developed method was successfully applied to detect PPCPs in marketed fish, and five PPCPs, including triclocarban, sulfadiazine, sulfadimidine, sulfamethoxazole, and carbamazepine, were detected at trace levels. The proposed method, which has the advantages of short analysis time, less solvent consumption, and high sensitivity, can be used for the determination of trace PPCPs in fish.

Effect of Pretreatment on Detection of 37 Pesticide Residues in Chrysanthemum indicum.

This study aimed to develop a method, followed by gas chromatography-mass spectrometry, for detecting 37 pesticides in Chrysanthemum indicum (C. indicum) and investigating the decrease in the matrix-induced enhancement effect. The influence of QuEChERS extraction and matrix solid-phase dispersion (MSPD) on the recovery and matrix effect (ME) was compared. extraction and matrix solid-phase dispersion (MSPD) on the recovery and matrix effect (ME) was compared to decrease the ME. The cleanup sorbents, volume and type of solvent, and treatment time were optimized. The accuracy (as recovery), precision (as relative standard deviation, RSD), linearity, limit of quantitation, and limit of detection were determined. The recoveries at the three levels using mixed standard solution ranged between 76% and 120% with RSD ≤15%, and 76% and 120% with RSD ≤11% for MSPD and QuEChERS extraction, respectively. The results suggested that the ME for 21 pesticides was in the range of 80%–120% after MSPD and 15% after QuEChERS extraction. QuEChERS extraction was simpler and faster than MSPD. This methodology was applied in the analysis of 27 C. indicum samples; phorate was most frequently detected (63.0% of the sample).

Boron nitride nanosheet-assisted matrix solid-phase dispersion microextraction of alkaloids from lotus plumule by high-performance liquid chromatography coupled with ultraviolet detection and ion mobility quadrupole time-of-flight mass spectrometry.

A boron nitride nanosheet (BNNS)-assisted matrix solid-phase dispersion method was established to microextract alkaloids from medicinal plants. The target compounds were identified by high-performance liquid chromatography coupled with ultraviolet detection and ion mobility quadrupole time-of-flight mass spectrometry. During the experimental process, several important parameters, including the type of dispersant, the amount of dispersant, the grinding time, and the type of elution solvent, were optimized. Finally, the BNNSs were chosen as the best dispersant, and their microcosmic morphologies were identified by scanning electron microscopy and transmission electron microscopy. Because of the special property of BNNSs, the cost of this experiment was greatly reduced, especially in elution volume, sample amount (50mg), and extraction time (2min). Under the best conditions, 50mg of sample powder was dispersed with 50mg of BNNSs, the grinding time was 120 s, the mixed powder was eluted with 200μL of methanol, and good linearity (r2 >0.9993) and satisfactory recoveries (80-100%) were obtained. The inter- and intraday precisions were acceptable, with RSDs lower than 2.01 and 4.84%, respectively. The limits of detection ranged from 2.54 to 15.00ng/mL, and the limits of quantitation were 8.47 to 50.00ng/mL. The proposed method was successfully applied for the determination of liensinine, isoliensinine, and neferine in lotus plumule.

Column-free matrix solid-phase dispersion extraction based on cucurbit[8]uril used for the determination of the flavonoid content in Oroxylum indicum (L.) Vent.

A simple, rapid and environmental method named column-free matrix solid-phase dispersion (FMSPD) has been investigated for the extraction of three flavonoids (oroxin A, oroxin B and baicalein) from the seeds of Oroxylum indicum (L.) Vent. In this study, cucurbit[8]uril was used as the sorbent of FMSPD to selectively extract three flavonoid compounds and transferred them into an aqueous solution for direct determination using HPLC-UV based on the recognition interaction of cucurbit[8]uril toward the compounds. The variables that influenced the extraction efficiency were investigated, which include the amount of cucurbit[8]uril, grinding time, volume of water, and pH of the solution. A good linearity was exhibited with correlation coefficients > 0.9991 for all three flavonoids. The intra- and inter-day precisions were 1.7–2.6% and 3.1–5.5%, respectively, and the limit of detection was between 0.1 and 0.18 μg mL–1. The recoveries ranged from 90.8% to 101.4%, with relative standard deviations of 2.9–6.8% under the optimized conditions. The developed method was applied to analyze four kinds of Semen Oroxyli from different regions of China. F- and t-tests showed that the results had no significant difference when comparing the traditional ultrasound-assisted extraction method to the novel FMSPD extraction method, but the method was simpler, faster and did not use organic solvents.

Performance of different extraction methods for paralytic shellfish toxins and toxin stability in shellfish during storage.

Accurate analysis of paralytic shellfish toxins (PSTs) in shellfish is important to protect seafood safety and human health. In this study, the performance of different extraction protocols for PSTs from scallop tissues is compared and discussed, including regular extraction solvents hydrochloric acid (HCl) and acetic acid (AcOH) followed by heating and solid-phase extraction (SPE) purification, and a novel technique of matrix solid-phase dispersion (MSPD) without heating. The possible conversion of C1/2 and GTX2/3 standards after heating, and the stability of PSTs in wet scallop tissues stored at -20°C for a 6-month period are also explored. Results showed that the MSPD technique could effectively mitigate matrix interference, but its recoveries of PSTs were significantly lower than those of the HCl and AcOH extraction methods followed by carbon SPE purification. The molar concentrations of M-toxins obtained by the MSPD method were generally lower than those analyzed by the HCl and AcOH extraction methods, which demonstrated a weak chemical conversion of C1/2 and GTX2/3 due to the heating process. Most of the PSTs were relatively stable in scallop tissues during 1-month storage at -20°C, while the concentrations of PSTs in scallop tissues obviously changed after 6 months due to the degradation and transformation of PSTs during long-term storage at -20°C. This work helps improve our understanding of the performance of different extraction methods and the stability of PSTs in scallop tissues stored at -20°C.

Fast determination of phthalates in mussel samples by micro-matrix solid-phase dispersion (micro-MSPD) coupled with GC\u2013MS/MS

A fast, effective and low cost sample preparation method based on miniaturized matrix solid-phase dispersion (micro-MSPD) combined with gas chromatography coupled to tandem triple-quadrupole-mass spectrometry (GC–MS/MS) has been developed for the determination of six phthalate diesters (DMP, DEP, DBP, BzBP, DEHP and DnOP) in mussel samples. The six target compounds have been included in the list of priority pollutants by United States Environmental Protection Agency. The extraction step was optimized on real spiked mussel coming from Galician Rías by means of a factorial design. The final procedure involved the use of 0.45 g of sample, 0.5 g of dispersant agent (Florisil) and 3 mL of organic solvent (ethyl acetate). The optimized method was validated giving satisfactory analytical performance, low detection limits (0.09 to 6.73 ng g−1 dw) and high recoveries (93 and 114%). The validated method was applied to four real mussel samples coming from Galician Rías.

Hydrophilic-interaction-based magnetically assisted matrix solid-phase dispersion extraction of carbadox and olaquindox in feeds.

Carbadox and olaquindox have been banned from feeds since 1998 by the EU because of their mutagenic, photoallergic, and carcinogenic effects. Unfortunately, owing to their outstanding effect, they are frequently abused or misused in animal husbandry. There is an urgent need to develop a sensitive and reliable method for monitoring these drugs in animal feeds. This work reported a new method of hydrophilic-interaction-based magnetically assisted matrix solid-phase dispersion (MMSPD) extraction coupled with reversed-phase liquid chromatography-mass spectrometry for simultaneous determination of carbadox and olaquindox in animal feeds. 3-Trimethoxysilylpropyl methacrylate (γ-MAPS)-modified attapulgite (ATP) was crosslinked with γ-MAPS-modified iron(II,III) oxide (Fe3 O4 ), 1-vinyl-3-(butyl-4-sulfonate) imidazolium (VBSIm), acrylamide (AM), and N,N'-methylene-bis(acrylamide) (MBA) to synthesize ATP@Fe3 O4 @poly(VBSIm-AM-MBA) particles. The resultant particles were characterized by scanning electron microscopy, energy dispersive spectrometer, transmission electron microscopy, vibrating sample magnetometer, and Fourier transform infrared spectroscopy. Crosslinking of ATP into the magnetic particles has significantly increased the adsorption capacity of the particles. Under optimum conditions, the limits of detection (S/N=3) were 0.3μg kg-1 and 0.9μg kg-1 for carbadox and olaquindox respectively. The intra-day and inter-day recoveries of the spiked targets in feed samples were in the range 83.5-98.3% with relative standard deviations of 1.0-8.3%. With a simplified procedure and a low amount of sample, the proposed hydrophilic-interaction-based MMSPD method is not only useful for the determination of carbadox and olaquindox in feeds but also holds great promise for the analysis of other polar targets in solid or semisolid matrices. © 2021 Society of Chemical Industry.

Occurrence of Fungicides in Vineyard and the Surrounding Environment.

Seventeen fungicides were determined in different matrices from vineyard areas, including vine leaves, soils, grapes and water, using gas chromatography coupled to tandem mass spectrometry (GC-MS/MS). For leaf analysis, ultrasound-assisted extraction (UAE) was performed evaluating different solvents. UAE was compared with other extraction techniques such as vortex extraction (VE) and matrix solid-phase dispersion (MSPD). The performance of the UAE method was demonstrated on vine leaf samples and on other types of samples such as tea leaves, underlining its general suitability for leaf crops. As regards other matrices, soils were analyzed by UAE and microwave-assisted extraction (MAE), grapes by UAE and waters by SPE using cork as the sorbent. The proposed method was applied to 17 grape leaf samples in which 14 of the target fungicides were detected at concentrations up to 1000 μg g−1. Furthermore, the diffusion and transport of fungicides was demonstrated not only in crops but also in environmental matrices.

Rapid Screening Alpha-Glucosidase Inhibitors from Polygoni Vivipari Rhizoma by Multi-Step Matrix Solid-Phase Dispersion, Ultrafiltration and HPLC.

Polygoni Vivipari Rhizoma (PVR), the dried root of Polygonum viviparum, has been used as herbal medicine in China for a long time. In the present study, a new method based on multi-step matrix solid-phase dispersion (MSPD), ultrafiltration and high performance liquid chromatography (HPLC) for screening alpha-glucosidase inhibitors (AGIs) from PVR was proposed. First, three different PVR extractions were prepared by multi-step MSPD with 15% methanol, 60% methanol and 100% methanol. Second, the alpha-glucosidase inhibition tests for the three extracts were carried out, and the 60% methanol extraction showed the best activity. Then, the AGIs screening experiment was performed with ultrafiltration and HPLC analysis using the 60% methanol extraction. Seven binding components (quercetin−3−O−vicianoside, quercetin 3−O−neohesperidoside, rutin, hyperoside, quercetin 3−O−glucuronide, luteolin−7−O−neohesperidoside, kaempferol 3−glucuronide) were found. These seven components were further validated as the AGIs by molecular docking analysis. The developed method was a rapid and efficient tool for screening AGIs from PVR, which provided scientific data for the bioactive components study of PVR.

Matrix solid-phase dispersion based on cucurbit[7]uril-assisted dispersive liquid–liquid microextraction coupled with high performance liquid chromatography for the determination of benzimidazole fungicides from vegetables

A new method involving matrix solid-phase dispersion (MSPD) and dispersive liquid–liquid microextraction (DLLME) was optimized with the aid of stoichiometry and applied to the extraction, purification, and determination of benzimidazole residues in vegetables. Carbendazim, thiabendazole, and thiophanate-methyl were selectively extracted from vegetables using cucurbit[7]uril as the MSPD extractant and transferred to an aqueous solution, then further enriched using DLLME with acetonitrile and chloroform as dispersive and extraction solvents, respectively. The optimal extraction conditions of MSPD and DLLME were selected by two-level full-factorial design and central-composite design (CCD). The developed method (MSPD–DLLME–HPLC–UV) showed good linearity in the range of 0.025–5 μg/g, with R2 > 0.9984. Intra- and interday precisions were 5.3–10.9% and 10.6–12.4%, respectively, and the limit of detection was between 0.004 and 0.007 μg/g of fresh weight. This method was applied to the analysis of four different types of vegetables, and the recoveries ranged from 65.4% to 124.0%. The method was environmentally friendly, easy to operate, and sensitive.

Numerical design of the filtering process of a matrix solid-phase dispersion based extraction plant using computational fluid dynamics and the discrete element method

Matrix solid-phase dispersion is a common procedure for extracting biochemical components from plants. For this process, the size of the particles of organic materials is reduced using a pretreatment to increase the mass transfer area. Afterwards, elution and filtration are performed to extract secondary metabolites with the help of solvents. In the final step, the solvent is removed using a separation process in order to save it for future extractions, and to obtain the extract as final product. Here, a semi-automatic design using a numerical model based on the discrete element method and computational fluid dynamics is presented. The fluid dynamics is modeled during the filtering process to predict the particle concentration in the entire control volume. The results show that the proposed model exhibits an error of less than 2% relative to the experimental value, with potential use in industrial applications where Newtonian fluids interact with solid particles in the liquid or gas phase.

A sustainable vortex-assisted matrix solid phase dispersion for the simultaneous extraction of five active components from Saposhnikoviae Radix

Saposhnikoviae Radix (SR), one of top-grade herbs, is widely used to treat cold, headache, fevers, cough, rheumatism and cardiovascular diseases in prescriptions. A vortex-assisted matrix solid phase dispersion (VA-MSPD) extraction method applying a reusable dispersant was developed to extract four chromones and one coumarin from Saposhnikoviae Radix ( SR ) , which followed by high performance liquid chromatography analysis (HPLC). The novel dispersant, acetonitrile modified carbon-coated magnetite nanoparticles (Fe 3 O 4 @C@ ACN MNP), could be reused for 10 times and it still possesses excellent extraction performance. Various optimal experimental condition of VA-MSPD was considered and obtained reliable matrix linearity (r 2 > 0.999) and good recovery (94.8%–105%). In order to recovery the dispersant from sample-dispersant powder after elution, methanol/water (50:50, v/v) was applied as washing solution to separate the dispersant and samples, so that the percent recovery of modified materials was obtained as 88.6% (RSD = 0.74%). The results indicated that Fe 3 O 4 @C@ACN MNP could be considered as sustainable dispersant and it exhibited tremendous potential for efficacious extraction of chromones and coumarins. • Fe 3 O 4 @C@ACN MNP was firstly prepared, of which amide groups (NH 2 CO) was modified on the surface of Fe 3 O 4 @C MNP. • VA-MSPD was established applying Fe 3 O 4 @C@ACN MNP as dispersant. • The established method was to extract components from Saposhnikoviae Radix . • A novel washing method was first developed to recover the magnetic dispersant. • The recovered magnetic dispersant could be reused 10 times.

High diversity of natural Dalmatian pyrethrum based on pyrethrin composition at intra- and interpopulation level

Seeds collected from 10 Dalmatian pyrethrum (Tanacetum cinerariifolium /Trevir./ Sch. Bip. Asteraceae) populations naturally occurring along the Adriatic coast and islands, were used to establish a common garden with the aim of estimating the variation of pyrethrin compounds between and within populations. A recently optimized matrix solid phase dispersion (MSPD) method was used for the extraction of six pyrethrin compounds from dry flower heads of 200 Dalmatian pyrethrum individuals. Separation and quantification of pyrethrin compounds were performed by high performance liquid chromatography with diode array detector (HPLC-DAD). High variability in pyrethrin content and composition was observed within and between populations. Total pyrethrin content in individual samples varied from 0.10% to 1.35% of flower dry weight. On average, the lowest total pyrethrin content was observed in the population of Pelješac (0.22%) and the highest in the island populations of Mali Lošinj and Zlarin (0.87%). The population of Mali Lošinj had the most favourable pyrethrin profile, including the lowest variability in total pyrethrin content, the highest pyrethrin I (PI) content (up to 60.47%) and PI/PII ratio (up to 5.88), as well as the lowest pyrethrin II (PII) content. In contrast, the populations from Biokovo and Pelješac represented pyrethrin profiles with the lowest content of total pyrethrin, PI and PI/PII ratio. The Biokovo population also had the highest PII content (43.18% on average), while the Pelješac population had the highest jasmolin I (JI) and jasmolin II (JII) content. Four different chemotypes were determined by cluster analysis. Chemotype P1 is the most promising for future breeding programs and is characterized by the highest total pyrethrin content, PI content and PI/PII ratio, as important measures of insecticidal activity. Correlation analysis of each pyrethrin compound with 25 environmental variables and Principal Component Analysis (PCA) were carried out and the importance of optimum temperature and precipitation on pyrethrin quantity and quality was suggested. Total pyrethrin content was significantly correlated with six temperature and five precipitation parameters. Pyrethrin I, PII and cinerin II (CII) were significantly correlated mainly with temperature parameters; JII and cinerin I (CI) with precipitation parameters, while JI was correlated with temperature range and precipitation parameters. The mountainous Biokovo population was an outlier in the PCA analysis, mainly due to the extremely low values of temperature parameters at a higher altitude. Precipitation variables separated the central Adriatic islands inhabiting drier habitats from populations inhabiting habitats with greater precipitation. The high-resolution data from this study provide the opportunity for individual-based selection for breeding plants with the best pyrethrin profiles, as well as for increased adaptation to broader climatic conditions.

Magnetic Ionic Liquids in Sample Preparation: Recent Advances and Future Trends

In the last decades, a myriad of materials has been synthesized and utilized for the development of sample preparation procedures. The use of their magnetic analogues has gained significant attention and many procedures have been developed using magnetic materials. In this context, the benefits of a new class of magnetic ionic liquids (MILs), as non-conventional solvents, have been reaped in sample preparation procedures. MILs combine the advantageous properties of ionic liquids along with the magnetic properties, creating an unsurpassed combination. Owing to their unique nature and inherent benefits, the number of published reports on sample preparation with MILs is increasing. This fact, along with the many different types of extraction procedures that are developed, suggests that this is a promising field of research. Advances in the field are achieved both by developing new MILs with better properties (showing either stronger response to external magnetic fields or tunable extractive properties) and by developing and/or combining methods, resulting in advanced ones. In this advancing field of research, a good understanding of the existing literature is needed. This review aims to provide a literature update on the current trends of MILs in different modes of sample preparation, along with the current limitations and the prospects of the field. The use of MILs in dispersive liquid–liquid microextraction, single drop microextraction, matrix solid-phase dispersion, etc., is discussed herein among others.

Simultaneous determination of pesticides and their degradation products in potatoes by MSPD-LC-MS/MS

The matrix-solid phase dispersion coupled with ultra-high performance liquid chromatography-tandem mass spectrometry was developed and in-house validated to determine pesticides commonly used in crop protection programs dedicated to potatoes and other vegetables. Five pesticides (thiamethoxam, lambda-cyhalothrin, deltamethrin, rimsulfuron and metalaxyl) and five main degradation products were selected for in-house validation and quantification in potatoes. The method showed good accuracy with recovery values between 70–96 % and relative standard deviation lower than 10 % for all target analytes. The limit of quantification ranged between 0.002 mg kg-1 and 0.051 mg kg-1. The method was used to monitor the target compounds in fifteen potato samples bought at fifteen different local markets in Poland. In eight of the potato samples, at least one pesticide concentration exceeded the maximum residue limit established by the European Union, while in ten of the potato samples, at least one pesticide degradation product was detected. This is the first analytical method for simultaneous determination of pesticides and their degradation products in potatoes that fulfils SANTE/12682/2019 requirements.

Recent Advances in Molecular-Imprinting-Based Solid-Phase Extraction of Antibiotics Residues Coupled With Chromatographic Analysis

Abuse and residues of antibiotics cause great harm to organisms and the environment. Appropriate sample pretreatment is usually required for sensitive determination, because of the low content presence of a variety of antibiotics in complicated matrices. Molecular-imprinting-based solid-phase extraction (MISPE) has been widely used for sample pretreatment of antibiotics, using molecularly imprinted polymers (MIPs) as adsorbents. Herein, we comprehensively review the recent advances of MISPE of antibiotics, followed by chromatographic analysis. Various solid-phase extraction (SPE) modes based on MIPs are briefly introduced, such as conventional SPE, dispersive SPE, magnetic SPE, matrix solid-phase dispersion, and pipette-tip SPE. Then, several emerging preparation techniques for antibiotics MIPs are summarized including surface imprinting, nanoimprinting, living/controlled radical polymerization, multitemplate imprinting, multifunctional monomer imprinting, and dummy template imprinting. Subsequently, applications of MISPE to analysis of a variety of antibiotics residues since 2018 are overviewed, including sulfonamides, quinolones, tetracycline, and others. Finally, the preparation and application of antibiotics MIPs are prospected.

Binary Eluent Based Vortex-Assisted Matrix Solid-Phase Dispersion for the Extraction and Determination of Multicomponent from Musk by Gas Chromatography-Mass Spectrometry.

A green, flexible, and effective strategy was proposed to quantify four target compounds (muscone, ethyl palmitate, ethyl oleate, and ethylparaben) from musk by binary eluent based vortex-assisted matrix solid-phase dispersion (MSPD) extraction coupled with GC/MS. Single-factor tests and orthogonal design were employed to optimize the MSPD parameters. In addition, the binary eluent system, methanol, and ethyl acetate 3 : 7 (v/v) were used to extract the target analytes. Finally, C18 was applied as the easily available dispersant and the sample powder was ground for 2 min. Thereafter, the mixture was rapidly extracted with the binary eluents under whirling for 3 min. Eventually, the analysis of the samples was completed within 12 min by GC/MS. All correlation coefficients (r) of four targets were more than 0.9991. The recoveries of all target compounds ranged from 92.8% to 101% while their RSDs were less than 6.94%. There was no significant matrix interference for the analysis. Thus, the combination of vortex-assisted MSPD with GC/MS was considered as a novel, rapid, and environmentally friendly quantitative approach for musk samples.

Impact of Different Extraction Methods on Furanosesquiterpenoids Content and Antibacterial Activity of Commiphora myrrha Resin.

The oleo-gum-resin of Commiphora myrrha is one of the most known natural antimicrobial agents, mainly due to its furanosesquiterpenes. A validated method based on sample extraction by matrix solid-phase dispersion (MSPD) followed by high-performance column chromatography (HPLC) determination is applied to analyze two furanosesquiterpenoids, namely, 2-methoxyfuranodiene (CM-1) and 2-acetoxyfuranodiene (CM-2), existing in C. myrrha. The trial parameters that controlled the extraction prospective were studied and optimized. These include the nature of dispersant, mass ratio of sample to the dispersant, and the volume of elution solvent. A comparative antimicrobial study that used the Minimum Inhibitory Concentration Assay (MIC) method between MSPD, ultrasonic, and Soxhlet of myrrh extracts was also conducted. The optimal MSPD parameters used were (i) 15 mL of methanol applied as elution solvent; (ii) silica gel/sample mass at a 2 : 1 ratio; and (iii) a dispersing sorbent selected as silica gel. Technique retrievals were regulated from 96.87% to 100.54%, with relative standard deviations (RSDs) from 1.24% to 4.45%. Commiphora myrrha-MSPD (CM-MSPD) extract showed the highest antibacterial activity against gram-positive and gram-negative bacteria (156.25 μg/mL and 312.5 μg/mL, respectively) and antifungal activity (156.25 μg/mL). Yields acquired through the MSPD technique were larger than yields from other extraction techniques (sonication and traditional reflux extraction methods) with less consumption of time, sample, and solvent. The mode of antibacterial action of CM-1 and CM-2 was elucidated by performing molecular docking with bacterial DNA gyrase. Both the compounds interacted with key residues of DNA gyrase.