Binary Eluent Based Vortex-Assisted Matrix Solid-Phase Dispersion for the Extraction and Determination of Multicomponent from Musk by Gas Chromatography-Mass Spectrometry.

Shanshan Wang,Jiading Guo,Ye Shang,Kunze Du,Chunxiao Liang,Jin Li,Tao Liu,Yan-Xu Chang,María José Trujillo-Rodríguez

doi:10.1155/2021/9913055

Abstract

A green, flexible, and effective strategy was proposed to quantify four target compounds (muscone, ethyl palmitate, ethyl oleate, and ethylparaben) from musk by binary eluent based vortex-assisted matrix solid-phase dispersion (MSPD) extraction coupled with GC/MS. Single-factor tests and orthogonal design were employed to optimize the MSPD parameters. In addition, the binary eluent system, methanol, and ethyl acetate 3 : 7 (v/v) were used to extract the target analytes. Finally, C18 was applied as the easily available dispersant and the sample powder was ground for 2 min. Thereafter, the mixture was rapidly extracted with the binary eluents under whirling for 3 min. Eventually, the analysis of the samples was completed within 12 min by GC/MS. All correlation coefficients (r) of four targets were more than 0.9991. The recoveries of all target compounds ranged from 92.8% to 101% while their RSDs were less than 6.94%. There was no significant matrix interference for the analysis. Thus, the combination of vortex-assisted MSPD with GC/MS was considered as a novel, rapid, and environmentally friendly quantitative approach for musk samples.

Highlights

Musk, as a precious aromatic-type traditional medicine, is obtained from the dried secretion of mature male Moschus berezovskii Flerov, Moschus sifanicus Przewalski, and Moschus moschiferus Linnaeus principally distributed in Northwest and Northeast of China
The shrinkage of suitable habitats made the numbers of wild musk dramatically reduce under the adverse effects of the natural environment and hunting [9]. e bad impact of the musk reduction led to the lack of natural musk. erefore, it is of great necessity to enhance the management system of artificially culture musk and improve the quality of artificial musk
In the matrix solid-phase dispersion (MSPD) step, the solid dispersant has two important effects on the sample including mechanical damage and adsorption capacity [19]. e analyte was exposed to the surface of the sample by grinding and dispersing the sample with dispersant, and the exposed substance was transferred by the dispersant. e interaction between the target compound and packing may be hydrogen bonding and van der Waals forces [27]. erefore, C18, C8, Florisil, HXN, and PEP were investigated to select superior dispersants

Summary

Introduction

As a precious aromatic-type traditional medicine, is obtained from the dried secretion of mature male Moschus berezovskii Flerov, Moschus sifanicus Przewalski, and Moschus moschiferus Linnaeus principally distributed in Northwest and Northeast of China. Muscone has made a great contribution to brain injury because it could enhance the permeability of the blood-brain barrier (BBB) [1] and protect the neurons [2] through prolonging the resistance to hypoxia. It had good performance in the treatment of diabetic neuropathy, antiinflammatory, and analgesic activities [3,4,5]. The study of quality assessment is more focused on muscone in musk and its Chinese patent medicines

Methods

Results

Conclusion