Hyperthyroid Group Research Articles

Effect of Ghrelin on Epididymal Sperm Analysis and Antioxidant System in Hyperthyroidic Adult Male Rats

The study's objective is to evaluate the enhanced effect of Ghrelin (GRE) on sperm analysis and the antioxidant system in male rats after inducing hyperthyroidism by L–thyroxin. The rats (50 males) were split into five groups. The first group consisted of 10 male rats that were given normal saline for 30 days S.C and set as a control group. While the second group (positive group) was 10 male rats with hyperthyroidism were given normal saline for 30 days S.C, the third group was 10 male rats with hyperthyroidism were given Ghrelin at a dose of (0.5nmol/100μl saline) for 30 days S.C, and the fourth group was male 10 rats with hyperthyroidism were given Ghrelin (1nmol/100μl saline) for 30 days S.C. The fifth group consisted of 10 hyperthyroid male rats that were given Ghrelin at a dose of (2nmol/100μl saline) for 30 days S.C. The results show a significant (P≤0.05) decrease in all parameters of sperm analysis, the number of births and antioxidative system in hyperthyroid group compared to control group. While, all treated groups with GRE (0.5, 1 and 2 nmol) shows enhance effect in sperm analysis, the number of births and antioxidant system compared to the hyperthyroid group.

The molecular evaluation of thioredoxin (TXN1 & TXN2), thioredoxin reductase 1 (TXNRd1), and oxidative stress markers in a binary rat model of hypo- and hyperthyroidism after treatment with gallic acid

Oxidative stress plays an important role in the pathology of thyroid disorders. This study examined the effect of gallic acid (GA) on the oxidative status and expression of liver antioxidant genes including thioredoxin (TXN1 & TXN2) and thioredoxin reductase1 (TXNRd1) in hypo- and hyperthyroid rat models. Forty-nine male Wistar rats were randomly assigned into seven groups as follows: control group, hypothyroid and hyperthyroid groups respectively induced by propylthiouracil and levothyroxine, hypo- and hyper thyroid-treated groups (where the groups were separately treated with 50 and 100 mg/kg of GA daily, orally). The levels of thyroid hormones and serum oxidative stress markers were evaluated after 5 weeks. The relative expression of TXN1,2 and TXNRd1 genes was measured via real-time qRT-PCR. The mean level of total antioxidant capacity (TAC), malondialdehyde, and uric acid index diminished in the hypothyroid group. Increased TAC reached almost the level of control in hypothyroid groups treated with GA. Elevation of thiol index in the hypothyroid group was observed (p < 0.01), which diminished to the control level after GA treatment. The relative expression of TXN1, TXNRd1, and TXN2 genes in the hypothyroid and hyperthyroid groups significantly increased compared to the control group (p ≥ 0.05), but in the groups treated with GA, the expression of these genes declined significantly (p ≥ 0.05). Our results indicated GA can affect the expression of TXN system genes in the rat liver. Also, the results suggest GA has a more positive effect on modulating serum oxidative parameters in hypothyroid rat models than in hyperthyroid.

Comparison on the consistency of Mindray and Siemens chemiluminescence analyzers for detecting FT3, FT4 and TSH in patients with hyper- and hypothyroidism.

To examine the consistency of the Mindray and Siemens full-automatic chemiluminescence analyzers in detecting serum free triiodothyronine (FT3), free thyroxine (FT4) and thyroid stimulating hormone (TSH) in patients with hyper- or hypothyroidism. Included in this study were 164 new patients with abnormal thyroid function assessed at Nan Fang Hospital of Southern Medical University in 2021: 107 patients with hyperthyroidism and 57 with hypothyroidism. Additional 100 healthy individuals comprised the control group. Consistency of the FT3, FT4 and TSH results from the two systems in the different groups was analyzed by Kappa, linear correlation, regression, Bland-Altman and area under the receiver operating characteristic curve (AUC). Compared with the control group, FT3 and FT4 levels were increased and TSH level was decreased in the hyperthyroid group (both P<0.05), whereas FT3 and FT4 levels were decreased and TSH level was increased in the hypothyroidism group (both P<0.05), suggesting that both test systems could provide references for relevant clinical evaluation. Kappa analysis showed high consistency of the two systems in detecting FT3, FT4 and TSH in both hyper- and hypothyroidism patients (mean Kappa value >0.7). In addition, there was a good linear correlation between the test results of the two systems (R2>0.90). Bland-Altman consistency analysis showed that most mean difference values were within the consistency limit (x±1.96s), indicating that the difference between the two systems was acceptable. AUC of FT3, FT4 and TSH detected by the two systems was higher than 0.80 in each group, showing no significant difference between them (both P>0.05), and the difference in sensitivity and specificity was within the acceptable range. Mindray and Siemens chemiluminescence analyzers are highly consistent in detecting FT3, FT4 and TSH in both hyper- and hypothyroidism patients.

Thyroid Dysfunction Among Patients with Systemic Lupus Erythematosus in Saudi Arabia

AbstractSystemic lupus erythematosus (SLE) is an autoimmune disease that may be associated with other autoimmune disorders. To date, limited data are available on thyroid dysfunction and SLE in Saudi Arabia. In this retrospective study, we reviewed the cases of 151 patients with SLE. The prevalence of thyroid dysfunction was 17.2%: 4% in the hyperthyroid group and 11.9% in the hypothyroid group. Euthyroid sick syndrome was found in 1.3% of patients. Moreover, 57% of patients with hypothyroidism were positive for antibodies to thyroglobulin and thyroid peroxidase. No correlation was found between the presence of thyroid dysfunction and higher SLE disease activity, according to the Safety of Estrogens in Lupus National Assessment–Systemic Lupus Erythematosus Disease Activity Index (SELENA-SLEDAI) score.

N-Acetylcysteine, an ROS Inhibitor, Alleviates the Pathophysiology of Hyperthyroidism-Induced Cardiomyopathy via the ROS/Ca2+ Pathway

Hyperthyroidism is common and can induce cardiomyopathy, but there is no effective therapeutic strategy. The purpose of this study was to investigate the molecular mechanism of hyperthyroidism-induced cardiomyopathy (HTC) and the effect of N-acetylcysteine (NAC), an ROS inhibitor, on the pathophysiology of HTC in vivo and in vitro. Compared with those in the control groups in vivo and in vitro, TT3 and TT4 were significantly increased, the structure of myocardial cells was enlarged and disordered, and interstitial fibrosis and the apoptosis of myocardial cells were markedly increased in the L-Thy group. The ROS and inflammatory response were increased in the hyperthyroidism group. In the NAC group, the contents of TT3 and TT4 were decreased, the myocardial cell structure was slightly disturbed, fibrosis and apoptosis were significantly reduced, and the ROS level and inflammatory response were significantly reduced. Interestingly, L-Thy decreased the viability of fibroblasts and H9c2 cells, suggesting that L-Thy-induced fibrosis was not caused by the proliferation of fibroblasts. The molecular mechanism of HTC could be explained by the fact that L-Thy could cause cardiac hypertrophy, inflammation, and fibrosis by regulating the Ca2+/calpain/Rcan1-dependent signalling pathway, the Ca2+/Rcan1/NF-κB/p65-dependent signalling pathway, and the Ca2+/ROS/Bcl-2/caspase-3-dependent signalling pathway. In conclusion, NAC can alleviate the pathophysiology of hyperthyroidism-induced cardiomyopathy, probably by regulating the ROS/Ca2+-dependent pathway.

Insinuation of Extrapolative Factors of Diagnostic Importance in Breast Cancer Patients Experiencing Thyroid Dysfunction

Background: Breast cancer (BC) is the most common malignancy in women and now regarded as the commonest cancer overall. Over 2 million new cases were diagnosed in 2018, accounting for almost 25% of cancer cases among women. The main objective of the study is to find the insinuation of extrapolative factors of diagnostic importance in breast cancer patients experiencing thyroid dysfunction. Materials and methods: This correlational study was conducted on Jinnah Hospital patients with the permission of ethical committee of the hospital and consent of the patients. The data was collected from 88 cases and 50 controls. All the patients who had a definite diagnosis of BC along with, hyperthyroidism, hypothyroidism, autoimmune thyroid disease (AITD), or thyroid cancer were included in this study. The study population was divided into three comparative groups: (1) controls (n = 50), (2) BC cases with hypothyroidism (n = 27), and (3) BC cases with hyperthyroidism (n = 61). Results: The mean age was 47.325±4.59 years for controls, 51.59±8.59 years for the hypothyroid cases, and 50.59±5.58 years for the hyperthyroid cases. The mean systolic blood pressure (SBP) for controls, hypothyroid and hyperthyroid was 121.25±6.58 mmHg, 98.259±8.59 mmHg and 129.65±7.59 mmHg respectively. The P-values less than 0.05 were considered to be significant (table 01). The demographic and hematological data of control, hypothyroid and hyperthyroid groups was tabulated. A positive correlation of breast cancer in women with hyperthyroidism and a slightly negative correlation in women with hypothyroidism was found. Conclusion: There appears to be an association between the thyroid function level and breast cancer risk. It can be extrapolated that anxiety associated with hyperthyroidism may play a role in stress induced BC. Therefore, thyroid function should be monitored in patients at risk of BC. Keyword: Breast cancer, Thyroid, Dysfunction, Hypothyroidism, Hyperthyroidism, Anxiety.

Thyroid hormones and glycaemic indices in euthyroid, hyperthyroid, hypothyroid, all type 2 diabetics and non-diabetic subjects

BackgroundAbnormal thyroid hormone levels have been reported in type 2, but the relationship between thyroid hormone levels and glycaemic indices: fasting plasma glucose (FPG), C-peptide (C-pep) and glycated haemoglobin (HbAlc), used in the management of type 2 is not well defined. This cross-sectional study examined the relationship between thyroid hormones and glycaemic indices in type 2.ResultsPositive correlations were observed between FPG and HbAlc in hypothyroid (r = 0.382, P = 0.011) and hyperthyroid group (r = 0.295, P = 0.012). FPG correlated with C-pep in hyperthyroid diabetics (r = 0.481, P < 0.001). HbAlc and TSH correlated positively in hypothyroid diabetics (r = 0.330: P = 0.031). HbAlc also correlated with T4 in hypothyroid diabetics (r = 0.379: P = 0.012). C-peptide and TSH correlated positively in hyperthyroid diabetics only (r = 0.279; P = 0.042). C-peptide also correlated with T3 and T4 in euthyroid diabetics (r = 0.231, P = 0.020; r = 0.248, P = 0.045), respectively.ConclusionThe presence of abnormal levels of thyroid hormones influenced glycaemic indices in type 2 population. This implies that thyroid hormones investigation can assist in proper diagnosis and management of diabetes mellitus.

Attaining biochemical euthyroidism early after total thyroidectomy in Graves' disease may lower long-term morbidity risk.

BackgroundThe relationship between good early control of thyroid hormone levels after thyroidectomy for Graves’ disease (GD) and subsequent risks of mortality and morbidities is not well known. The aim of this study was to examine the association between thyroid hormone levels within a short interval after surgery and long-term mortality and morbidity risks from a population-based database.MethodsPatients with GD who underwent complete/total thyroidectomy between 2006 and 2018 were selected from the Hong Kong Hospital Authority clinical management system. All patients were classified into three groups (euthyroidism, hypothyroidism, and hyperthyroidism) according to their thyroid hormone levels at 6, 12, and 24 months after surgery. Cox proportional hazards models were performed to compare the risks of all-cause mortality, cardiovascular disease (CVD), Graves’ ophthalmopathy, and cancer.ResultsOver a median follow-up of 68 months with 5709 person-years, 949 patients were included for analysis (euthyroidism, n = 540; hypothyroidism, n = 282; and hyperthyroidism, n = 127). The hypothyroidism group had an increased risk of CVD (HR = 4.20, 95 per cent c.i. 2.37 to 7.44, P < 0.001) and the hyperthyroidism group had an increased risk of cancer (HR = 2.14, 95 per cent c.i. 1.55 to 2.97, P < 0.001) compared with the euthyroidism group. Compared with patients obtaining euthyroidism both at 6 months and 12 months, the risk of cancer increased in patients who achieved euthyroidism at 6 months but had an abnormal thyroid status at 12 months (HR = 2.33, 95 per cent c.i. 1.51 to 3.61, P < 0.001) and in those who had abnormal thyroid status at 6 months but achieved euthyroidism at 12 months (HR = 2.52, 95 per cent c.i. 1.60 to 3.97, P < 0.001).ConclusionsThis study showed a higher risk of CVD in postsurgical hypothyroidism and a higher risk of cancer in hyperthyroidism compared with achieving euthyroidism early after thyroidectomy. Patients who were euthyroid at 6 months and 12 months had better outcomes than those achieving euthyroidism only at 6 months or 12 months. Attaining biochemical euthyroidism early after thyroidectomy should become a priority.

Investigation of Coxsackie Virus Type B in Grave’s Thyroiditis

Thyroiditis is a broad term that refers to a variety of clinical conditions marked by inflammation and damage to thyroid cells. These disorders can be chronic or acute, include lymphocytic infiltration, have a microbial cause, or be linked to autoimmune thyroid disease, such as in Graves’ disease (GD). This cross-sectional study aimed to detect anti-TSH antibodies and investigate Coxsackie virus B (CVB) IgM and IgG antibodies in the serum of patients to know their role in the pathogenesis of thyroiditis. The study included 91 patients suffering from thyroid dysfunction through thyroid hormone tests (FT3, FT4 and TSH) by Snibe Maglumi-800 Chemiluminescence Immunoassay, where they were divided into three groups (subclinical thyroiditis, hypothyroidism and hyperthyroidism). To determine which of them has Graves’ disease, detection of anti-TSH Ab have been performed then detection of antibodies to specific CVB IgM and IgG in the sera of patients by ELISA. The results of serology showed that the percentage of anti-TSH antibodies in the three groups was (68.13%), and the percentage was the highest in the hyperthyroidism group, which amounted to (85%). The serological diagnosis also showed that the presence of antibodies to CVB IgM was (65.93%) while, IgG levels were (20.87%). Conclusions: Coxsackie virus B infection is commonly seen in patients with thyroiditis and may have a role in the its pathogenesis. Keywords: Coxsackie virus B, Graves’ disease, Thyroiditis

Anxiety in patients with hyperthyroidism

IntroductionMental symptoms are the first manifestations of hyperthyroidism. They include anxiety, dysphoria, irritability, emotional lability, sleep disorders, intellectual dysfunction, mania or depression. Anxiety is the main symptom and requires more detailed study.ObjectivesThe objective was to determine symptomatology of anxiety in patients with hyperthyroidism and compare with euthyroid patients.MethodsThe study included 56 patients with hyperthyroidism (high free T3 and free T4, suppressed TSH) and 32 euthyroid patients (normal free T3, free T4 and TSH) of the control group. For psychiatric assessment State-Trait Anxiety Inventory [STAI], Hamilton Depression Rating Scale [HAM-D], and Hamilton Anxiety Rating Scale [HAM-A] were used.ResultsTotal scores obtained from STAI, HAM-D and HAM-A were significantly greater in the hyperthyroidism group than that of the euthyroid group (p&lt;0.05). The level of state anxiety in patients with hyperthyroidism was 51.39 ± 0.95 (high level) compared with 41.59 ± 2.41 (moderate level) in the control group. The level of trait anxiety in patients with hyperthyroidism was 46.86 ± 0.69 (high level), and 44.16 ± 2.17 (moderate level) in the control group. Psychomotor agitation (HAM-D # 9), psychic anxiety (HAM-D # 10), insomnia (HAM-A # 4) and weight loss (HAM-D # 16) were typical for patients with hyperthyroidism, while in the control group predominate the feelings of fatigue, weakness and loss of interest in working (HAM-D # 7).ConclusionsThe prevalence of anxiety in patients with hyperthyroidism is significantly more frequent compared to euthyroid patients. Anxiety and other psychiatric symptoms should be considered by both endocrinologists and psychiatrists.DisclosureNo significant relationships.

Gonadotropin profile in experimentally induced hypothyroid and hyperthyroid cyclic female rats

The current study examined the association of thyroid disorders with reproductive dysfunction by determining its effect on gonadotropin secretion in cyclic female rats. Sixty cyclic females were assigned to three groups (20 each) and supplemented, for 30 days plus two consequent estrous cycles, with drinking water (control), methimazole in drinking water (0.02% w/v) (hypothyroid group), and thyroxine in drinking water (0.002% w/v) and gastric gavage of 200 μg/kg body weight (hyperthyroid group). At late proestrus, ten females from each group (for each cycle) were anesthetized and dissected. Blood samples were obtained to assess thyroid-stimulating hormone, free and total triiodothyronine, free and total thyroxin, follicle-stimulating hormone, luteinizing hormone, and prolactin concentrations. Ovarian and pituitary tissue samples were obtained for molecular analysis of ovarian thyroid receptor genes and pituitary TSH, FSHβ, and LHβ genes. In comparison with control, the Hypo group revealed increased serum concentrations of TSH and PRL and the expression levels of pituitary TSH and ovarian TRsTRs genes and significant decrease of FT3, TT3, FT4, TT4, FSH, and LHLH concentrations and the expression levels of pituitary FSHβ and LHβ genes. In contrast, the Hyper group showed increased serum FT3, TT3, FT4, TT4, and LHLH concentrations and the expression levels of pituitary LHβ and ovarian TRsTRs genes and decreased serum TSH FSH and PRL concentrations and pituitary FSHβ and TSH gene expression levels. It is concluded that thyroid dysfunction is associated with altered serum gonadotropin secretion and reproductive failure.

Determination of the Level of IL-6 and Vaspin in Hyperthyroid Patients Treated with Carbimazole

The level of adipokines (interleukin-6 and vaspin) in hyperthyroid patients treated with carbimazole drug was determined. Eighty-five male and female participated in this study, with mean age from 20-70 years. They included: negative control group of thirty euthyroid persons represented group 1, twenty-five hyperthyroid patient (positive control) included in the second group and thirty hyperthyroid patients treated with carbimazole drug for one year or less comprised the third group. By vein puncture, blood was collected, and serum was isolated and preserved at –20 C. Adipokines (interleukin-6 and vaspin) were estimated by using ELISA method. The results demonstrated that the the increase of IL-6 level was highly significant (p&lt;0.01) in hyperthyroid group as compared with euthyroids. But the result showed a significant decrease in vaspin level in hyperthyroid group with treatment as compared with hyperthyroid group. However, there was a significant (p&lt;0.05) decrease in the level of vaspin in hyperthyroid group with treatment in comparison to euthyroids. Also there was no significant difference in vaspin level in hyperthyroid group with treatment in comparison to hyperthyroid group. These findings suggest that the level of IL-6 increase in hyperthyroid patient and decrease after treatment with carbimazole drug for one year or less. Although vaspin level decreased in hyperthyroid patients, it decreased even after treatment with carbimazole drug for one year or less.

Comparative Clinicopathological Evaluation of the Ocular Surface in Newly Diagnosed Patients of Hyperthyroidism and Hypothyroidism Compared to Healthy Subjects.

IntroductionThyroid eye disease is a multifactorial disorder affecting the tear film and ocular surface, which leads to dry eye. The aim of the study is to evaluate the tear function test and ocular surface changes clinically and to correlate pathologically using impression cytology in recently diagnosed cases of thyroid dysfunction, including both hyperthyroid and hypothyroid patients, and to compare the results with healthy subjects.MethodsThis was a cross-sectional analytical study. Thirty diagnosed patients with hyperthyroidism and hypothyroidism, each within three months, and 30 healthy age-matched controls were included. All patients and controls underwent an assessment of proptosis, palpebral fissure height (PFH), tear function test, Ocular Surface Disease Index (OSDI) questionnaire, Schirmer’s test, tear break-up time (TBUT), and impression cytology for ocular surface assessment. These parameters were compared between cases and controls. Standard statistical analysis was used.ResultsMean proptosis and PFH showed no significant difference among groups (P-value = .071). The mean value of OSDI was 42.33+22.67, 41.15+16.03, and 29.33+6.84 in the hyperthyroid, hypothyroid, and controls groups, respectively; the difference being statistically significant (p=0.001). Mean TBUT was 7.13+3.28 sec, 6.38+2.46 sec, and 11.15+2.39 sec in hyperthyroidism, hypothyroidism, and controls, respectively (p=0.001). The mean value of the Schirmer tear test was 12.93+5.81 mm, 13.30+4.44 mm, and 17.55+7.35 mm in hyperthyroidism, hypothyroidism, and controls, respectively (p=0.001). Eighty percent (80%) of patients in the hyperthyroidism group had grade 2-3 squamous metaplasia as compared to 70% in hypothyroidism patients and 24.4% in controls, signifying ocular surface damage (p<0.05).ConclusionsThe mean PFH and proptosis did not differ between the three groups. However, increased OSDI score, decreased Schirmer’s test value, decreased TBUT, and grades 2-3 squamous metaplasia in patients with thyroid dysfunction suggest the presence of dry eyes. Despite no clinically visible signs of thyroid ophthalmopathy, there is ocular surface damage right from the early stages of thyroid dysfunction, possibly attributable to the evaporative mechanism as well as ocular surface inflammation and instability of the tear film.

The Relationship Between Thyroid Diseases and Irisin Hormone

Introduction: The aim of this study is to investigate the relationship between irisin, a new test with similar effects on energy metabolism and thermogenesis, and thyroid hormones. Material and Methods: 86 subjects included in the study. The subjects were divided into three groups as euthyroid, hypothyroid and hyperthyroid group by evaluating their TSH and fT4 levels. For all subjects, gender, age, medical history and blood test results were examined. Irisin levels of all groups were measured. Statistical analysis was performed with the obtained data. Results: A total of 86 people; 30 patients with newly diagnosed hypothyroidism, 28 patients with newly diagnosed hyperthyroidism and 28 euthyroid individuals as the control group were included in the study.The mean irisin level was highest in the hyperthyroid group and lowest in the hypothyroid group. The mean irisin level was found to be significantly higher in the hyperthyroid group than in the hypothyroid and control group (p˂0.01).The irisin level was found to be significantly higher in the control group than in the hypothyroid group (p˂0.01). A statistically significant negative correlation was found between the irisin levels and TSH levels (p˂0.01). A statistically significant positive correlation was found between the irisin levels and fT4,fT3 levels (p˂0.01). Conclusion: A significant correlation has been found between the TSH,fT3,fT4 and irisin.It was found that as TSH levels increase, irisin levels decreased and that as fT3, fT4 levels increase, irisin levels increased.We believe that irisin can be used as a new test in the follow-up and diagnosis of thyroid diseases, with studies with larger sample size and long-term follow-up in future.

Dyslipidemia In Thyroid Disorders

Background: Thyroid hormones play an important role in regulating lipid metabolism; and thyroid dysfunctions can result in lipid abnormalities which increase the risk of endothelial dysfunction, hypertension and cardiovascular disease.&#x0D; Methods: A cross-sectional study was conducted on 50 patients with suspicion of thyroid disorders were taken as cases. 50 patients with normal thyroid profile and no history of other chronic diseases were taken as control group.&#x0D; Results: The serum TC, TG and LDL levels in hypothyroid individuals (both overt and subclinical) were significantly higher than euthyroid subjects but the levels were comparable between hyperthyroid and euthyroid group.&#x0D; Conclusion: We conclude that, dyslipidemias are associated with thyroid disorders, so biochemical screening for thyroid dysfunction in all dyslipidemic patients. Therefore, patients presenting with dyslipidemia are recommended for investigation to explore thyroid dysfunction.&#x0D; Keywords: Total cholesterol, Triglycerides and LDL.

Menopausal Impact on the Association Between Thyroid Dysfunction and Lipid Profiles: A Cross-Sectional Study.

BackgroundBoth dyslipidemia and thyroid dysfunction have a high prevalence rate and are important risk factors for cardiovascular diseases. However, the relationship between blood lipids and thyroid dysfunction is still controversial. This study aims to analyze the blood lipids in people with different thyroid functions.MethodsA total of 80937 adults were included in this population-based cross-sectional TIDE survey, which collected demographic and clinical data on thyroid function, blood lipid levels and other metabolic indicators. After screening, the final analysis included 10,747 participants, who were divided into hypothyroidism (n=311), subclinical hypothyroidism (n=5015), hyperthyroidism (n=203), subclinical hyperthyroidism (n=118) and control (n=5100) groups. The risk of dyslipidemia was analyzed by a logistic regression model and divided into groups of female menopausal.ResultsAfter full adjustment, significant associations were found between hypothyroidism and hypertriglyceridemia. Subclinical hypothyroidism was associated with a significantly higher risk of hypertriglyceridemia and hyper-low density lipoprotein cholesterolemia. Hyperthyroidism was significantly correlated with a reduced risk of hypercholesterolemia and hyper-low density lipoprotein cholesterolemia but positively correlated with the risk of low-high density lipoprotein cholesterolemia. There was no significant association between subclinical hyperthyroidism and blood lipids. Hypothyroidism increased the risk of hypertriglyceridemia in both premenopausal and postmenopausal females. Subclinical hypothyroidism was significantly associated with increased hypertriglyceridemia and low-high density lipoprotein cholesterolemia in premenopausal females. Hyperthyroidism was significantly associated with a reduced risk of hypercholesterolemia and hyper-low density lipoprotein cholesterolemia in premenopausal females and an increased risk of low-high density lipoprotein cholesterolemia in postmenopausal female.ConclusionAbnormal thyroid function has an important effect on blood lipids and is closely related to female menopause.

The effect of selenium on learning disability due to maternal hyperthyroidism during lactation

Aim: Hyperthyroidism causes adverse changes in hippocampal-dependent learning and memory functions in developmental and adult periods. In the present study, the healing effect of selenium on learning disability in the offspring of mothers given L-thyroxine during lactation was investigated. Material and Methods:12 pregnant rats were used in the study. The pregnant rats were randomly assigned into three groups (n=8 per group): control group (CG), lactational hyperthyroid group (LHG), and lactational hyperthyroid + selenium group (LH+SeG) group. LHG consisted of male rat offspring from these mothers treated with L-thyroxine (0.2 mg kg–1 body mass, 1 mL) during lactation. LH+SeG was induced by selenium supplementation during 39-60 days after lactational hyperthyroid. The offspring of mother rats administered saline during the lactation period formed the control group (CG). Spatial learning and memory function were measured with the Morris water maze (n = 16 for each group). Measurements were made when offspring rats were 60-66 days old. Results: Post-hoc test with one-way ANOVA revealed that LHG group rats found the platform by traveling longer distances and swimming longer than the control group (p 0.005). There was no significant difference between the groups in terms of the time spent in the target quadrant in the probe trial (memory test). Conclusion: The study findings show that high thyroid hormone levels in mother rats during lactation have long-term negative effects on hippocampal-dependent learning function in offspring rats and selenium has a healing effect on these disorders.

Bone mineral density (BMD) and neutrophil-lymphocyte ratio (NLR), monocyte-lymphocyte ratio (MLR), and platelet-lymphocyte ratio (PLR) in childhood thyroid diseases.

This study aimed at investigating the laboratory parameters related to the pathogenesis of bone loss, including bone mineral density (BMD), neutrophil-lymphocyte ratio (NLR), monocyte-lymphocyte ratio (MLR), and platelet-lymphocyte ratio (PLR) in children with thyroid disease and healthy controls. Children and adolescents with hypothyroidism (n=63) and hyperthyroidism (n=30) as well as 32 age- and sex-matched healthy controls were included in the study. Auxological data, BMD, hemogram parameters, the levels of thyroid hormone, thyroid stimulating hormone (TSH), thyroid autoantibodies, parathyroid hormone, 25-hydroxy vitamin D, alkaline phosphatase, calcium, and phosphorus were analyzed. The mean age of the patients was 12.12±2.7 years (range: 8-17). BMD Z-scores were within the normal range in all the patients and healthy controls. The BMD Z-scores were significantly higher in patients with hyperthyroidism than those in the control group and in patients with hypothyroidism. No significant difference was observed between the control and hypothyroid groups in terms of the BMD Z-scores. A correlation was observed between the BMD Z-scores and NLR, MLR, PLR, and free T4 levels. In patients with hypothyroidism, the BMD Z-scores were significantly positively correlated with the NLR, MLR, PLR, and the TSH level. In the control group, there was a moderate positive correlation between the BMD Z-scores and NLR. In the hyperthyroid group, there were no significant correlations between the BMD Z-scores and other variables. The study data suggest that in children and adolescents with thyroid disease, the relationship between the BMD Z-scores and NLR, MLR, and PLR at the initial diagnosis in the hypothyroidism group was different from that in their healthy peers.

Hyperthyroidism in Graves Disease Causes Sleep Disorders Related to Sympathetic Hypertonia.

It is well known that Graves disease (GD) causes sleep disorders (SDs). However, the characteristics and associated factors of SD and its clinical course post hyperthyroidism normalization remain unclear. To clarify the characteristics and associated factors of subjective SD and its clinical course after GD treatment. From November 2017 to October 2020, we enrolled 72 participants (22 newly diagnosed with GD with untreated hyperthyroidism, 20 previously diagnosed with GD with normal thyroid function, and 30 normal controls) with no other underlying SD-related diseases. We compared the groups at enrollment and conducted prospective observations after 12 months of treatment on participants with newly diagnosed GD. Main outcome measures were differences and changes in the Pittsburgh Sleep Quality Index (PSQI) global and component sleep quality scores. PSQI global sleep quality scores (P = .036) and sleep disturbance scores (P = .011) were significantly different among the 3 groups, and were highest in the untreated hyperthyroidism group. Multiple regression analysis demonstrated that free thyroxine level, which was positively correlated with sympathetic tone (ST) as evaluated by pulse rate, and urinary total metanephrines was associated with poorer PSQI global sleep quality scores independently of other factors (P = .006). Prospective observation showed that PSQI global sleep quality scores (P = .018) and sleep disturbance scores (P = .011) significantly improved with thyroid function normalization and ST attenuation. Hyperthyroidism caused by GD augmented ST and exacerbated subjective SD. Normalization of hyperthyroidism caused by GD improved subjective SD.

Interplay Between Thyroid Hormone Status and Pulmonary Hypertension in Graves' Disease: Relevance of the Assessment in Thyrotoxic and Euthyroid Patients.

BackgroundGraves’ disease (GD) is the most common cause of hyperthyroidism and can cause cardiac changes, such as pulmonary hypertension.MethodsThis is a prospective study in which we obtained demographic, clinical, laboratory data and characteristics of the GD, in addition to investigating cardiorespiratory function, focusing on the detection of pulmonary hypertension. Patients were separated into two groups: thyrotoxicosis and euthyroidism. Ninety patients with GD of both sexes, over 18 years of age, were included. The cardiorespiratory assessment included an echocardiographic evaluation, a questionnaire of specific symptoms, spirometry and a six-minute walk test.ResultsThe hyperthyroid group included 42 patients (47.73%) and the euthyroid group 46 patients (52.27%); 78 were women (86.67%). The prevalence of pulmonary hypertension between the hyperthyroidism (48.57%) and the euthyroidism (29.41%) groups was not different. Free thyroxine levels (FT4) (OR 1.266), higher left atrium volume (OR 1.113) and right ventricle diameter were associated with pulmonary hypertension. A direct correlation between FT4 with forced vital capacity (FVC) and forced expiratory volume in the first second (FEV1), as also an inverse correlation between initial oxygen saturation (SpO2) with diagnostic time and drop SpO2 with the ratio between the diastolic velocity E of the mitral flow and the diastolic velocity of the mitral ring (E/e’) were observed in the euthyroid group. An inverse correlation between FT4 levels with walked distance as % of predicted value, and a direct correlation between E/e’ ratio and walked distance as % of predicted value were observed in the hyperthyroid group.ConclusionWe emphasize the importance of a cardiorespiratory reassessment in GD, even after a long-term control of the thyrotoxic state, as we demonstrate that about 30% of these patients remain with PH and are subject to specific treatment.