Antibacterial Activity Of Silver Nanoparticles Research Articles

Characterization and Antibacterial Activity of Silver Nanoparticles Biosynthesized Using Leaves Extract of Artemisia sieberi and Calotropis procera

The prevalence of antibiotic-resistant bacteria has increased recently leading to the need for novel, natural antibacterial agents such as plant-synthesized silver nanoparticles. Such synthesis is safe, cheap, rapid, non-toxic and environmentally friendly. In this study, characterization of biosynthesized silver nanoparticles from extracts of A. sieberi and C. procera was carried out using transmission electron microscopy, fourier transform infrared and energy dispersive x-ray analysis. Spherical nanoparticles with an average size was ~10 nm for A. sieberi and ~14 nm for C. procera were synthesised; synthesis was most effective using A. sieberi. Antibacterial activity of silver nanoparticles was carried out using the agar-diffusion method and by determination of the minimum inhibitory concentration. Biosynthesized silver nanoparticles showed antibacterial activity against Staphylococcus aureus, MRSA, Salmonella typhimurium and Escherichia coli, with silver nanoparticles extracts from A. sieberi being the most antibacterial.

Evaluation antioxidant and antibacterial activities of silver nanoparticles synthesized by aqueous extract of Pistacia atlantica

Silver nanoparticles AgNPs were synthesized from an extract of Pistacia atlantica plant. After mixing the silver nitrate solution and extract of P. atlantica leaves, they changed its color to dark brown. UV–vis spectroscopy result showed maximum adsorption at 468 nm, which represents the characteristic surface plasmon resonance of nanosilver. FTIR spectroscopy results recorded a downward shift of absorption bands between 500 and 1700 cm−1 indicting the formation of silver nanoparticles. X-ray crystal analysis showed that the silver nanoparticles are highly crystalline and exhibit a cubic, face-centered lattice with characteristic (111), (200), (220), and (311) orientations. Composition analysis obtained from SEM–EDXA confirmed the presence of elemental signature of silver. Results also showed that biosynthesized AgNPs exhibit remarkable antioxidant activity using DPPH method with IC50 = 0.47 mg/ml. In addition, synthesized AgNPs were also found to control the growth of E. coli, S. aureus and Acinetobacter baumnnii on nutrient medium with 13, 12 and 11 mm mm zone of inhibition, respectively. The main compounds that were identified in the methanolic and the ethanolic extracts of P. atlantica Desf are ascorbic acid, gallic acid, tannic acid, rutin, and quercetin which were performed by HPLC technique. The AgNPs synthesized from the leaf extract of P. atlantica can be a good method for the preparation of various nano formulations.

Formulation and Characterization of Anti-Bacterial Activity of Silver Nanoparticles Using Root and Leaf of Wedelia trilobata

In the present study one such species, Wedelia trilobata, has be chosen to review the In vitro antibacterial activity of the root extract by using solvent ethanol for the selected plant Wedelia triolobata. The deduction of nanoparticles used to be unalterable by UV-Visible spectroscopy, FTIR, particle size, and zeta potential. The anti-bacterial activity of Wedelia trilobata mediated synthesis silver nanoparticles was tested by disc diffusion assay against standard organisms like Escherichia coli and staphylococcus aureus bacteria. The formation of silver nanoparticles by Wedelia trilobata was initially demonstrated by color changes confirmed by UV-Visible root exhibited a specific absorbance peak around 450 nm and leaf exhibited a specific absorbance peak around 418nm respectively. The Zeta potential of silver nanoparticles was found to be root 0.1 and leaf 0.4, so it indicates the dispersion and stability. The antibacterial activity against Gram-positive Staphylococcus aureus was increased, which was indicated by an increase in the inhibition zone diameter from 3Mm for normal (9.5Mm), standard (12.3Mm), control (11.3Mm), extraction for AgNPs Wedelia trilobata (13.8). 5Mm for normal (7.3Mm), standard (19.4Mm), control (10.5Mm), extraction for AgNPs Wedelia trilobata (19.8). The antibacterial activity against Gram-negative E.coli was increased, which was indicated by an increase in the inhibition zone diameter from 3Mm for normal (7.8Mm), standard (8.5Mm), control (10.2Mm), extraction for AgNPs Wedelia trilobata (14.3). 5Mm for normal (13Mm), standard (19.6Mm), control (15.3Mm) extraction for AgNPs Wedelia trilobata (19.8). The present investigation revealed that the ethanolic Root and aqueous leaf extracts the chosen plant have potential to suppress the expansion of infective bacterial strains.

Bionanoparticles from Moringa as Antimicrobial Activity and It’s Application in Water Purification: Thesis Abstract

Thesis Abstract Background: Nanotechnology is the greatest promising technique for generating new applications in water purification. Green synthesis of silver nanoparticles and copper oxide nanoparticles is an eco-friendly, cheap and non-toxic more than chemical and physical methods. Aim: This study focuses on green synthesis, characterization, antibacterial activities of silver nanoparticles and copper oxide nanoparticles synthesized using Moringa oleifera leaf and stem extract and its application in water purification. Materials and Methods: The green synthesis of silver nanoparticles was done by using Moringa oleifera leaf and stem extract and 1mM of silver nitrate solution while copper oxide nanoparticles were synthesized by using Moringa oleifera leaf and stem extract and 10mM of copper sulfate pentahydrate solution. pH, temperature and the mixture reaction time are parameters affecting the formation of silver nanoparticles and copper oxide nanoparticles. Results: In this study, various techniques and devices used to characterize and confirms the formation of silver nanoparticles and copper oxide nanoparticles included visual observation, UV-Vis spectroscopy, TEM, FTIR, and EDX. Silver nanoparticles and copper oxide nanoparticles showed antimicrobial activity against E. coli ATCC 8739 and S. typhi ATCC 14028 and isolated microorganisms from tested water sample which included Pseudomonas aeruginosa and Klebsiella variicola. Cellulose filter paper which coated with silver nanoparticles and copper oxide nanoparticles showed good effect in water purification as its good antimicrobial activity against microorganisms, increasing pH value and reducing conductivity, total alkalinity values and Pb concentration value. Conclusion: The results from this study indicate that bio-nanoparticles from moringa have an antimicrobial activity that can be applied in water Purification

An Investigation on the Antiproliferative and Antibacterial Activity of Silver Nanoparticles of Quercus infectoria and Daucus carota subsp sativum

Plant mediated fabrication of nanoparticles and nanomaterials are gaining momentum as it is eco-friendly and cost-effective. In the present study, we synthesis of Silver nanoparticles using aqueous extract of Quercus infectoria nuts and Daucus carota subsp sativum leaves. The surface plasma resonance at 417 and 450 nm for Q. infectoria and D. carota respectively confirmed the formation of AgNPs. Scanning Electron Microscopic (SEM) confirmed the spherical shape of the nanoparticles, which had an average size of 67.5 nm and 49.2 nm for Q. infectoria nanoparticles (QAgNPs)and D. carota nanoparticles (DAgNPs). The elemental composition by Energy-Dispersive X-ray analysis of the nanoparticle showed an atomic percentage of silver as 73.64 % and 75.93% for Q. infectoria and D. carota.FT- IR analysis of the plant extracts and synthesized silver nanoparticles showed the presence of various functional groups. The total antioxidant activity of QAgNPs was 81.18% and that of DAgNPs was 73.36%. The QAgNPs and DAgNPs exhibited antibacterial activity against B. subtilis, E. coli and S. aureus. The percentage of cell viability for QAgNPs and DAgNPs assessed using HeLa cells was 21.1% and 6% respectively.

Biosynthesis of silver nanoparticles using Bacillus subitilis and its antibacterial activity

Background: Silver nanoparticles (AgNPs) have recently been extensively investigated because of their superior physical, chemical, and biological characteristics, and their superiority is primarily due to the size, shape, composition, crystallinity, and structure of AgNPs compared to their bulk forms. Objective: The current study aimed to investigate the antibacterial activity of silver nanoparticles (AgNPs) synthesized intracellular by using standard strain Bacillus subitilis ATCC 6633 against reference strains Escherichia coli ATCC 2592 and Staphylococcus aureus ATCC 29737. Results: The synthesized AgNPs showed potent antibacterial activities against the two tested bacterial strains with inhibition zones ranged from 42 -52mm and MIC 27.2 µg / ml. The silver nanoparticles were characterized with particle size ≃ 100 nm and zeta potential -19. There was deformation in both tested strains upon treatment with AgNPs which was observed by Scanning Electron Microscopy (SEM). Conclusion: The results indicated that AgNPs could be used as an effective antibacterial agent.

Ag@ZnO Nanoparticles Induce Antimicrobial Peptides and Promote Migration and Antibacterial Activity of Keratinocytes.

Antibacterial activity of silver nanoparticles is often associated with toxicity to the host. We here report that noncytotoxic doses of silver nanoparticles coated with zinc oxide, Ag@ZnO, can stimulate proliferation and migration of human keratinocytes, HaCaT, with increased expression of Ki67 and vinculin at the leading edge of wounds. Interestingly, Ag@ZnO stimulates keratinocytes to produce the antimicrobial peptides hBD2 and RNase7, promoting antibacterial activity against both extracellular and intracellular Staphylococcus aureus isolated from wounds. Overall, these results suggest that Ag@ZnO has the potential to significantly improve treatment outcomes in clearing wound infection.

Sunlight irradiation‐assisted green synthesis, characteristics and antibacterial activity of silver nanoparticles using the leaf extract of Jasminum subtriplinerve Blume

Silver nanoparticles (AgNPs) were biosynthesized at room temperature under sunlight irradiation, using the extract of Jasminum subtriplinerve Blume (JS) leaf as a reducing agent and also stabilizers. The obtained AgNPs were thoroughly characterized by UV–Vis Spectroscopy, X-ray diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), Fourier-transform infrared spectroscopy (FT-IR) and their antibacterial property was evaluated on five types of bacteria by implementing the minimum inhibitory concentration test (MIC) and the zone of inhibition test. No antibacterial activity was observed in the JS extract compared to the AgNPs; meanwhile, AgNPs exhibited effective antibacterial activity against all bacteria with an average diameter of inhibition zones of over 10.0 mm and the minimum inhibitory concentration of 33.08 µg/mL (higher inhibiting activity against gram (−) bacterial than that of gram (+) bacterial).

Green Synthesis and Characterization of Silver Nanoparticles using Crude Extract of Crotalaria burhia

Background: Appearance of antibiotic resistance has raised the demand to find alternative therapies and modified drug delivery system of medicinal plants to treat bacterial infections.
 Objective: The aim of this study is the green synthesis and characterization of silver nanoparticles by using crude extract of Crotalaria burhia and to evaluate their antibacterial potential.
 Methods: The roots and stems of plant were used to prepare the crude extract. The phytochemical analysis of different compounds in extract was performed. 1mM AgNO3 and different concentrations of plant extract were used for the green synthesis of silver nanoparticles. The particles size and zeta potential were measured by zeta sizer while surface morphology of silver nanoparticles was observed with Scanning Electron Microscope (SEM). The antibacterial activity of silver nanoparticles was performed by 96 well microdilution plate method.
 Results: The particle size and zeta potential of optimized formulation was 92 nm and -24.8 mV. The SEM analysis showed that silver nanoparticles are irregular and spherical shape. The antibacterial activity showed that MIC value of silver nanoparticles was lower for E. coli than S. aureus.
 Conclusion: Silver nanoparticles possess potent bactericidal activity against E. coli and moderate activity against S. aureus. It had been concluded that these nanoparticles can be used against multi-drug resistant bacterial infections.

Antibacterial activity of Green Synthesis of Silver Nanoparticles from Withania Somnifera (Ashwagandha) Root Extract

Biosynthesis (green synthesis) of metal nanoparticles such as Silver Nanoparticles (AgNPs) has been oneof the safest, most cost-effective and environmentally sustainable method in recent years. In this research,AgNPs were synthesized using Withania Somnifera (Ashwagandha roots). For the characterization ofsynthesized AgNPs different techniques were used, such as X-ray diffraction (XRD), Ultraviolet Visible(UV-VIS) spectroscopy, Fourier transform Infra-Red (FTIR) spectroscopy, scanning electron microscopy(SEM), Energy Dispersive X-ray (EDX) analysis, and atomic force microscope (AFM(. X-ray diffractionanalysis showed that the particles were crystalline, while in nature. UV-Visible absorption spectra of thereaction medium containing silver nanoparticles showed maximum in the visible region at 430 nm. FTIRanalysis confirmed the reduction of Ag+ ions to Ag0 ions in synthesized silver nanoparticles. The SEManalysis the size 5 nm shape and spherical in structure. The antibacterial activity of silver nanoparticles wasperformed on the growth of both gram-positive and gram-negative bacteria. A bactericidal was observedthrough the highest zone of inhibition against the bacterial strains, with higher activity for Meropenem. Theroot extract of Ashwagandha quickly reduces Ag+ to Ag0and enhances the synthesis of silver nanoparticleswith highly significant antibacterial activity.

Antimicrobial coating of fabric by biosynthesized silver nanoparticles from Panchakavya

Silver nanoparticles can be synthesized biologically by means of microbes and plants since they offer eco-friendly, non-toxic and uniform nanoparticles, though many researchers have done major work on biosynthesis there is very less research carried out on synthesis of nanoparticles by Panchakavya. Panchakavya is an Indian eco friendly fertilizer. Silver nitrate is treated with panchakavya to synthesize silver nanoparticles where in panchakavya acts as a reducing agent. The synthesized silver nanoparticles are monitored by UV-spectrophotometer then subjected to structural and morphological studies by XRD, SEM and AFM and silver nanoparticles of size 20–35 nm were obtained. The silver nanoparticles are then used against napkins containing urine sample for antimicrobial studies. Then the napkins are examined for the growth of colonies by carrying out bacterial culture method. The comparison study was done between the napkin without silver nanoparticles and with silver nanoparticles by using colony counting process. The antibacterial activity of silver nanoparticles were traced for two weeks and found same activity which was shown initially. Hence antimicrobial activity of AgNPs lasted even after weeks in napkin used.

Green Synthesis and Investigation of Antibacterial Activity of Silver Nanoparticles Using Eryngium bungei Boiss Plant Extract

Metal nanoparticles synthesis using plant extracts and the study of their medicinal effects is a relatively new topic. Many chemical techniques have been proposed for the synthesis of silver nanoparticles (AgNPs), but green synthesis has a special place due to its clean and non-toxicity. Eryngium bungei Boiss plant was selected for this study due to its reducing properties and medicinal properties. The effect of various factors including concentration of silver nitrate, contact time and pH were investigated to reach optimum conditions. Silver nanoparticles were synthesized by the reaction between extract and silver nitrate after 20 min at room temperature and pH 12. The obtained nanoparticles were analyzed by XRD, UV–Vis, FESEM, EDX analysis to determine the crystals, morphology, identification of constituents, identification of unknown substances and particle distribution, respectively. The absorption of silver nanoparticles at 438 nm was measured using spectrophotometer machine. Particle size, morphology and antibacterial activity against standard strains of Escherichia coli (E. coli; ATCC® 25922™), Klebsiella pneumoniae (K. pneumonia; PTCC® 700603™) and Staphylococcus aureus (S. aureus; PTCC® 16538™) were investigated and the results showed that the synthesized silver nanoparticles have antibacterial properties. Due to the antibacterial results, biosynthesized silver nanoparticles enhanced antibacterial efficiency against S. aureus, K. pneumonia and E. coli bacteria with MIC value of 27.34, 27.34, 6.83 µg/ml and MBC value of 54.68, 109.3 and 13.67 µg/ml, respectively. The results of this study showed that the synthesis of silver nanoparticles can be performed using the extract of Eryngium bungei Boiss and the synthesized nanoparticles by this extract have effective antibacterial effects.

Green Synthesis, Characterization and Antibacterial Activity of Silver Nanoparticles from Capparis Spinosa Leaf Extract

The current study reports the green synthesis of silver nanoparticles (AgNPs) using Capparis spinosa leaf extract acting as a capping and reducing agent. The characterization of AgNPs was confirmed using ultraviolet-visible spectrophotometry (UV-Visible), fourier transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). The plant extract used reduces Ag+ into AgNPs within a few minutes as indicated by the changed color, from yellow to reddish-brown. The UV-vis spectrum of AgNPs appeared a characteristic surface plasmon resonance peak at 400-450 nm. FTIR spectroscopy confirmed the role of plant extract as a reducing and capping agent of silver ions. The spectra of FTIR revealed a broad transmission peaks from 3412 to 617 cm-1. An EDX analysis signal at 3 keV and weight 65.38% showed the peak to be in the silver region, a fact which was confirmed by the presence of elemental silver. Under TEM, the nanoparticles were seen to be spherical, with an average particle size of 13 nm. AgNPs showed antibacterial activity against S.epidermidis, S. aureus, MRSA and E. coli. The inhibition zones for S.epidermidis and S. aureus were 8 to 10 mm, while MRSA is 7 to 10 mm. The inhibition zone of E. coli was higher at 10 to 13 mm.

Green Synthesis of Silver Nanoparticles from the Extracts of Fruit Peel of Citrus tangerina, Citrus sinensis, and Citrus limon for Antibacterial Activities.

Wide application of nanoparticles motivates the need for synthesising them. Here, a nontoxic, eco-friendly, and cost-effective method has been established for the synthesis of silver nanoparticles using extracts of lemon peel (Citrus limon), green orange peel (Citrus sinensis), and orange peel (Citrus tangerina). The synthesised nanoparticles have been characterised using UV-visible absorptionspectroscopy, Fourier transform infrared spectroscopy, and transmission electron microscopy (TEM). The UV-visible absorption spectrum of these synthesised silver nanoparticles shows an absorption peak at around 440 nm. TEM images show different shaped particles with various sizes. Furthermore, the antibacterial activity of silver nanoparticles was appraised by a well-diffusion method and it was observed that the green synthesised silver nanoparticles have an effective antibacterial activity against Escherichia coli and Staphylococcus aureus. The outcome of this study could be beneficial for nanotechnology-based biomedical applications.

Antibacterial Activity of Green-Synthesized Silver Nanoparticles Using Areca catechu Extract against Antibiotic-Resistant Bacteria.

In this work, the antibacterial activity of silver nanoparticles (AgNPs) synthesized using Areca catechu extracts against three species of antibiotic-susceptible and three species of resistant bacteria was investigated. The effects of this plant were more promising when compared with other medicinal plants tested. The hydrothermal extract of Areca catechu was mixed with silver nitrate to synthesize AgNPs. The synthesized particle characteristics were analyzed by UV–Vis spectrophotometry, scanning electron microscopy (SEM), dynamic light scattering (DLS), and Fourier-transform infrared spectroscopy (FT-IR). Minimum inhibitory concentration and minimum bactericidal concentration tests were conducted to confirm antibacterial activity and the results showed that AgNPs synthesized using Areca catechu extracts effectively inhibited the growth of bacterial species. Moreover, the SEM images of the bacterial species treated with AgNPs synthesized with Areca catechu extracts showed that clusters of AgNPs were attached to the surface of the bacterial cell wall, which could induce destruction of the cell membranes. The results suggest that AgNPs synthesized with Areca catechu extracts have the potential to treat antibiotic-resistant bacteria known as the major cause of nosocomial infections.

Synthesis of Terminalia bellirica fruit extract mediated silver nanoparticles and application in photocatalytic degradation of wastewater from textile industries

Terminalia bellirica has many biological applications and their metal nanoparticles have no hazardous effect on the environment. A biological synthesis, photocatalytic and antibacterial activities of silver nanoparticles using ethanolic extract of Terminalia bellirica fruit extract is reported in this article. The diffraction light scattering and UV–Vis. Spectroscopy is used to characterize the synthesis of silver nanoparticles. The photocatalytic degradation of methylene blue is evaluated by UV–Vis. Spectroscopy. The disc diffusion method is used to find antibacterial activities. Silver nanoparticles synthesized by T. bellirica fruit ethanolic extract showed good photocatalytic activity as well as antibacterial activities. The results obtained in the study showed the relevance of nanobiotechnology in the purification of water.

Evaluation of the antibacterial activity of silver nanoparticles, doxycycline, collagen and their amalgamation – An in vitro study

Biosynthesis of silver nanoparticles (AgNPs) using aqueous root extract of Delphinium denudatum (Dd). The fabricated DdAgNPs were characterized using UV–vis spectroscopy, high resolution transmission electron microscopy (HRTEM). The spectrum of brownish yellow color colloid exhibited notable peak at 416 nm. The size of DdAgNPs was ranging from 40 to 85 nm and was spherical in shape. The advent of multi-resistant pathogens has imposed the search of new strategies. In this situation, we combined silver nanoparticles (DdAgNPs) with doxycycline (DO) and collagen to assess the potency of this hybrid as a bactericidal agent against Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli, Salmonella paratyphi, Shigella dysentriae and Vibrio cholera. DdAgNPs exhibited antibacterial activity against only two test organisms but combination with collagen exhibited strong antibacterial activity against all test organisms. Doxycycline showed no activity against Escherichia coli, Shigella dysentriae and Vibrio cholera but combination with collagen exhibited antibacterial activity towards all test organisms with increased zone of inhibition except Bacillus cereus. Synergistic action of DdAgNPs, doxycycline and collagen resulted in enhanced antibacterial effect. Moreover, simultaneous action of doxycycline and DdAgNPs has made it difficult for pathogenic bacteria to develop resistance and hence, this combinational therapy can be further studied to develop new formulations of DdAgNPs in conjunction with doxycycline and collagen.

Green synthesis of silver nanoparticles using Syngonium podophyllum leaf extract and its antibacterial activity

Aqueous leaf extracts play a vital role in the development of nanoparticles through the environmentally friendly route in nanotechnology. An aqueous solution of dry leaves of Syngonium podophyllum (Nephthytis, arrowhead) is mixed with the silver nitrate (AgNO3) precursor for the synthesis of silver nanoparticles (AgNPs), which serves as a reducing, capping, and stabilizing agent. The change in color from golden yellowish to dark brownish signified the synthesis of silver nanoparticles. The UV–visible spectroscopy, X-ray diffraction (XRD), and fourier transform infrared spectroscopy (FT-IR), all confirmed the reduction of the silver nanoparticles. The morphology of the silver nanoparticles was investigated by the Field emission scanning electron microscope (FE-SEM), and High-resolution transmission electron microscopy (HR-TEM). The extracted solution produces the maximum absorbance peak at 420 nm. HR-TEM suggested the biosynthesized nanoparticles are spherical in shape, of particle size ranges from 2 to 47 nm. The EDX analysis of silver nanoparticles confirmed the elemental synthesis of silver at 3 keV. The compounds existing in the Syngonium podophyllum extracts were analyzed using the FT-IR to recognize the nature of the capping agents in these leaf extracts. Antibacterial activity of silver nanoparticles has been demonstrated in this study. The well diffusion method was used to analyze antibacterial activity, and the 96 well plate method was used to test minimum inhibitory concentration (MIC) against bacterial pathogens (Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus).

Prevalence of Listeria monocytogens in Farm Milk and Some Dairy Products with Comparing its Sensitivity to Different Antibiotics and H2O2 Loaded on Silver Nanoparticles in Dakahlia Governorate, Egypt.

L. monocytogens is one of the major food-borne pathogens which can affect humans and a wide variety of animal species. It usually invades Milk and its products at several steps in the food chain, Listeriosis outbreaks remain an important problem globally. High resistance of L. monocytogens to the most of antibiotics due to misusing of it in both veterinary and human field, it necessitates searching about alternatives. Drug laden nanoparticles possibly will overwhelm the restrictions of conservative antibiotic actions related with poisonousness, unsuitable distribution, or enzymatic dilapidation. The aim of this research is to detect prevalence of this bacteria in farm milk and some milk products and to mark the result of H2O2 loaded on silver nanoparticles on Multidrug resistance strains of L. monocytogenes. Therefore, One hundred samples (25 farm bulk tank, 25 plain yoghurt and 50 fresh soft cheese (Talaga)) were haphazardly composed from dairy farms and different shops in different areas of Mansoura city, Dakahlia governorate. Egypt. The isolated strains of L. monocytogenes were evaluated phenotypically and genotypically for detection of virulence factors (invasive linked protein (iap), haemolysin (hylA) and actin polymerization protein (actA)) that were carried out by using multiplex PCR. Out of 100 samples 7 listeria monocytogenes isolates from Fresh soft cheese (Talaga) samples (14%). These 7 isolates had iap gene, while 6 isolates had hly gene and 5 isolates had actA gene. Also the three types of virulence genes were in four isolates at which a higher multiple antibiotic resistance between L.monocytogenes isolates. Assessment of antibacterial action of silver nanoparticles and hydrogen peroxide Antibacterial doings was assessed by broth micro-dilution test and our results revealed that H2O2/AgNPs with 6.25-3.13 µg/mL MIC values of the original concentrations 13 µg/mL MIC values of the original concentrations highly effective against L. monocytogens.

Antibacterial activity of silver nanoparticles synthesized from leaf and flower extracts of Galinsoga formosa

Antibacterial activity of silver nanoparticles synthesized from leaf and flower extracts of Galinsoga formosa