Antibacterial activity of Green Synthesis of Silver Nanoparticles from Withania Somnifera (Ashwagandha) Root Extract

Abstract

Biosynthesis (green synthesis) of metal nanoparticles such as Silver Nanoparticles (AgNPs) has been oneof the safest, most cost-effective and environmentally sustainable method in recent years. In this research,AgNPs were synthesized using Withania Somnifera (Ashwagandha roots). For the characterization ofsynthesized AgNPs different techniques were used, such as X-ray diffraction (XRD), Ultraviolet Visible(UV-VIS) spectroscopy, Fourier transform Infra-Red (FTIR) spectroscopy, scanning electron microscopy(SEM), Energy Dispersive X-ray (EDX) analysis, and atomic force microscope (AFM(. X-ray diffractionanalysis showed that the particles were crystalline, while in nature. UV-Visible absorption spectra of thereaction medium containing silver nanoparticles showed maximum in the visible region at 430 nm. FTIRanalysis confirmed the reduction of Ag+ ions to Ag0 ions in synthesized silver nanoparticles. The SEManalysis the size 5 nm shape and spherical in structure. The antibacterial activity of silver nanoparticles wasperformed on the growth of both gram-positive and gram-negative bacteria. A bactericidal was observedthrough the highest zone of inhibition against the bacterial strains, with higher activity for Meropenem. Theroot extract of Ashwagandha quickly reduces Ag+ to Ag0and enhances the synthesis of silver nanoparticleswith highly significant antibacterial activity.