Abstract
Biosynthesis (green synthesis) of metal nanoparticles such as Silver Nanoparticles (AgNPs) has been oneof the safest, most cost-effective and environmentally sustainable method in recent years. In this research,AgNPs were synthesized using Withania Somnifera (Ashwagandha roots). For the characterization ofsynthesized AgNPs different techniques were used, such as X-ray diffraction (XRD), Ultraviolet Visible(UV-VIS) spectroscopy, Fourier transform Infra-Red (FTIR) spectroscopy, scanning electron microscopy(SEM), Energy Dispersive X-ray (EDX) analysis, and atomic force microscope (AFM(. X-ray diffractionanalysis showed that the particles were crystalline, while in nature. UV-Visible absorption spectra of thereaction medium containing silver nanoparticles showed maximum in the visible region at 430 nm. FTIRanalysis confirmed the reduction of Ag+ ions to Ag0 ions in synthesized silver nanoparticles. The SEManalysis the size 5 nm shape and spherical in structure. The antibacterial activity of silver nanoparticles wasperformed on the growth of both gram-positive and gram-negative bacteria. A bactericidal was observedthrough the highest zone of inhibition against the bacterial strains, with higher activity for Meropenem. Theroot extract of Ashwagandha quickly reduces Ag+ to Ag0and enhances the synthesis of silver nanoparticleswith highly significant antibacterial activity.
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