Ulin General Hospital Research Articles

The Effect Of Self Efficacy Towards Independency Level Of Post-Stroke Patient In General Hospital Neuro Policlinic Ulin Banjarmasin

Background: Stroke is a disease of brain function disorder of the nerves in the form of local and global roomates have impact to physical and physcological. Stroke is the third causes of death in Indonesia and in South Kalimantan every 9.2 per mill areas, stroke cases had happened. There are 1258 cases in 2015 hospitalized in Ulin General Hospital.
 Purpose: To analyze the effect of self-efficacy on towards the Independence level of post-stroke patient in general hospital neuro policlinic Ulin Banjarmasin
 Methods: The methodology that use in this study is descriptive analytics, the total respondents in this study is 33 respondents. Collecting the data in this study is using the Stroke Self-Efficacy and Barthel index questionare. The Data Analyzed by Kendal Tau test 
 Result: According to this study to 33 respondent Obtained results. The respondents who have high self efficafy as 25 (75.7%), moderate self efficacy as many as seven respondents (21.2%) and has low self-efficacy is one respondents (3.0%) and to the independence levelof post-stroke patient variables who had Categorized to independence are 14 respondents (42.4%), need minimal assistance 5 respondents (15.2%), partially need assistance in three respondents (9.0%), dependece assistance is five respondents (15.2%) and total assistance in six respondents (18.2%). the analysis result by Kendal Tau get p value = 0.044. the strength of the correlation is r = 310 it was statethat signifficant there is a moderate correlation and the direction are positive correlation.
 Conclusion: There signifficant There is correlation between self-efficacy to the Independence level of post-stroke patient

Obstetrical and Non-Obstetrical Factors toward Uterine Prolapse Occurrence in Ulin General Hospital Banjarmasin on Januari – December 2017

Abstract: Uterine prolapse is a gynecological disease in women that emerge patients physically and psychologically. Uterine prolapse is defined as an uterus herniation inside or outside vagina as the result of the ligament and fascia failure that retain the uterus in its actual place. This research was to identify obstetrical and non-obstetrical factors in uterine prolapses occurrence in Ulin General Hospital Banjarmasin on period January until December 2017 using case control approach. The total sample was 80 medical record samples, which divided into 40 medical record samples of patient with uterine prolapse and 40 medical record samples of patient without the occurrence of uterine prolapse. The results using chi square analysis obtained non-obstetrical factors: age >60 years old (OR 6.67: 95%CI 2.44-18.21), BMI > 30 (OR 3.10: 95%CI 1.24-7.71), menopause (OR 21.00: 95%CI 6.11-72.18); and non-obstetrical factors: parity >4 / multiparity (OR 13.78: 95%CI 4.71-40.28), macrosomia (OR 7.15: 95%CI 2.65-19.34), vaginal delivery (OR 28.78: 95%CI 7.41-111.79). The results of logistic regression obtained non-obstetrical factors: age > 60th (Exp B 4.21 and sig. 0.004), menopause (Exp B 1.12 and sig. 0.001), and multiparity (Exp B 2.35 and sig. 0.016). The results of this analysis obtained that obstetrical and non-obstetrical factors were related to uterine prolapse occurrence, and the dominant factor was age > 60 years old, so it could be concluded that there was significant factor between obstetrical and non-obstetrical factors on uterine prolapse occurrence in Ulin General Hospital Banjarmasin. Keywords: uterine prolapse, obstetrical factor, non-obstetrical factor

The Relationship of Childbirth Preparation Based on Childbirth Preparation Card with Maternal Morbidity and Fetal Outcomes

This research aims to determine the level of primigravida childbirth preparation through childbirth preparation card (CPC) and its relationship with maternal morbidity, including postpartum hemorrhage and ruptured perineum, and fetal outcomes (APGAR score, Down score and infant mortality). The researcher used a descriptive-analytical method with a cross-sectional approach. Childbirth preparation is assessed using CPC. The research begins from January to April 2019. The sample in this study were mothers who gave birth in Ulin General Hospital of Banjarmasin, which was 100 respondents when the researchers conducted a study with sampling using a purposive sampling technique. There were 76 respondents (76%) stated that they were ready for the childbirth preparation, and 24 respondents (24%) were not ready for preparation. From 76 ready respondents, as many as 73 respondents were not bleeding (93.6%), 47 respondents did not experience perineal rupture (87.0%), 70 respondents with good APGAR score (97.2%), 70 respondents with good Down score (70%) and 74 respondents did not die (80.4%). Childbirth preparation was associated with the rate of postpartum hemorrhage (p<0.0001; OR 92.4), perineal rupture (p=0.005; OR 3.93), APGAR score (p<0.0001;OR 128.33), Down scores (p <0.0001; OR 128.33) and infant mortality (p=0.002; OR 12.33). Childbirth preparation is related to the incidence of postpartum hemorrhage, perineal rupture, APGAR score, Down score, and infant mortality.

Monocyte Lymphocyte Ratio, Platelet Lymphocyte Ratio, Neutrophil Lymphocyte Ratio as Prognostic Markers in Patients with Multidrugs Resistant Tuberculosis Treated at Ulin General Hospital Banjarmasin

Abstract: Multi Drugs Resistant Tuberculosis (MDR TB) is tubercolusis with the least resistance to rifampicin and isoniazid. The progresivity and side effects of MDR TB treatment can be monitored through clinical, imaging, and laboratory evaluation. Monocyte Lymphocyte Ratio (MLR), Neutrophil Lymphocyte Ratio (NLR), and Platelet Lymphocyte Ratio (PLR) are the inflammatory markers used as biomarkers of treatment progress in MDR TB. The purpose of this research was to determine the difference of MLR, NLR, and PLR value pre and post MDR TB therapy and to determine the best prognostic biomarker in the evaluation of MDR TB treatment progress at Ulin General Hospital in January-December 2017. This research was an analytic observational study with a cross-sectional design. The sample was 17 patients selected based on the inclusion and exclusion criteria. Results obtained from paired T-tests showed the decreasing of MLR, NLR, PLR value in MDR TB patients receiving TB therapy. PLR had a more role as inflammatory biomarker of MDR TB treatment with p = 0.000, compared to MLR (ρ = 0.01), and NLR ( ρ = 0.006). The conclusion is PLR was significantly the best prognostic biomarker for MDR TB treatment. Keywords: MDR TB, Monocyte Lymphocyte Ratio, Neutrophil Lymphocyte Ratio, Platelet Lymphocyte Ratio

Bacteriological Pattern and Antibiotic Resistance in Children with Urinary Tract Infection in Ulin General Hospital Banjarmasin, Indonesia

Bacteriological Pattern and Antibiotic Resistance in Children with Urinary Tract Infection in Ulin General Hospital Banjarmasin, Indonesia

The Effect of Self-Management Education on Distress of Type 2 Diabetes Mellitus Patients in Ulin General Hospital Banjarmasin, South Kalimantan, Indonesia

Abstract: The purpose of this study was to analyze the effect of diabetes self management education (DSME) on distress of type 2 diabetes melitus patients which is hospitalized in Ulin General Hospital. This Study used Pre-Experiment with One Group Pretest-Posttest Design. The sample size was 32 patients. The results showed that the average of emotional burden before and after DSME was decrease, with difference number 0.325. The result of Distress among healthcare services before and after DSME was increase (0.211). The result of distress due to diabetes management before and after DSME was decrease (0.119). The result of distress interpersonal relationship before and after DSME was up (0,021). The analysis used Wilcox on Sign Rank test with p= 0,135 (p&gt; 0,05) for emotional distress, p = 0,443 (p&gt; 0,05) for Distress healthcare worker, p = 0.656 (p&gt; 0.05) for distress due to diabetes management. P=0,527 (p&gt;0,05) for Distress interpersonal relationship. The conclusion was there is no significant difference of distress level before and after DSME. It is shown that there is no effect of DSME on distress among patients with diabetes mellitus. Keywords: Diabetes Mellitus, Distress, Diabetes Self-Management Education (DSME)

Ratio of Platelet-Limfosites and Leukosit Level as Indicators of Baby Feedback (APGAR Score) on Premature Rupture of Membrane Cases iIn Ulin General Hospital

Abstract: Premature rupture of membranes (PROM) has the potential effect to cause complications such as premature infants, neonatal sepsis, and intrauterine infection (chorioamnionitis). Some inflammatory markers have been investigated to detect PROM at their early stage and prevent the complications. Platelet lymphocyte ratio (PLR) and leukocytes are a simple, affordable inflammatory marker, and have been used to predict a variety of inflammatory conditions. This study aims to see the relationship between PLR and leukocytes with APGAR Score in the case of PROM. This study was an analytic observational study with a retrospective cross sectional design. Data from 80 samples showed PLR and leucocytes had no significant effect on APGAR value (p&gt; 0.05). Keywords: Platelet Lymphocyte Ratio, Premature rupture of membranes, APGAR score

Relationship Between Age, Sex, Sports Activities, Smoking Status and Obesity Status with Hypertension Incidents

Hypertension is a condition characterized by an increase in blood pressure and provide symptom that will have an impact on the incidence of disease such as stroke, coronary heart disease, and left ventricular hypertrophy. Factors that influence the occurrence of hypertension were divided into two groups, namely controllable risk factors such as stress, obesity, unbalanced diet, and lifestyle, and the risk factors that can not be controlled such as genetics, age, gender, and ethnicity. Based on a preliminary survey of the number of Patients with hypertension served Ulin General Hospital in 2011 as many as 80 Patients, as many as 181 Patients in 2012 and in 2013 as many as 192 Patients. Research carried out by the analytic observational method with of cross sectional approach to the sample amounted to 97. The instrument research used spreadsheet, microtoise, and scales underfoot. Dependent variable: hypertension, independent variables: age, sex, sports activity, smoking status, and obesity status. Data were analyzed with chi square test with a level of 95%. The results showed that 51.5% of all respondents were classified as low-risk age, 58.8% male, 64.9% had a risky sport activities, 76.3% of light smokers, 67% obese and 61.9% including hypertension. The results of data analysis with chi square test showed that age, sports activity, smoking status, and status of obesity are related with hypertension. While sex is not related with the incidence of hypertension.

POST AMPUTATION RESPONSE AND COPING OF DIABETES MELLITUS PATIENT IN ULIN GENERAL HOSPITAL BANJARMASIN

 DM disease is a health problem that is very important because it is associated with a high incidence of complications and high mortality. For most people the disease diabetes mellitus is a disease that is very worrying and the public aware of the magnitude of the potential dangers caused. For individuals who suffer from diabetes mellitus with post-amputation, the next life is a new round of challenges and changes, and will go through the process of coping with the change process. The  aim of this study was to explore various diabetic patients post-amputation responses and coping experienced. This study used a qualitative phenomenological study. Retrieving data using in depth interview in four participants were treated at Diabetic Foot Polyclinic Ulin General Hospital Banjarmasin equipped with a guidance interview and informed consent. The analytical method structure from Creswell into 6 steps. There are 2 theme depicting various responses post-amputation and  depicting various coping DM patients post-amputation. Sub-Theme in depicting various responses post-amputation namely: (1) Experiencing physical barriers, (2) Experiencing the changing role, (3) Experiencing the grieving process, and are 4 Sub-theme depicting various coping DM patients post-amputation namely: (1) More worship, (2) Accept the circumstances, (3) A strong Motivation, (4) social support.

FAKTOR ORGANISASI YANG MEMPENGARUHI KINERJA (STUDI PADA PERAWAT IGD RSUD ULIN BANJARMASIN)

Emergency Installation (EI) is the first place for patients to get emergency treatment. Successful EIs are not solely about the availability of support facilities, but also human resource performance measured from organizational factors, such as nursing management, leadership style, incentives, organizational structure and job design are included. The main purpose of this study is to partially and simultaneously test the influence of organizational factors on nurse performance with descriptive method with quantitative analytic approach. The sampling technique used a census approach of 33 nurses at the Emergency Installation, Banjarmasin Ulin Hospital. All data were obtained by distributing questionnaires and analyzed by using multiple regressions. The result of data analysis shows that nursing management, transformational style, and work design have positive but not significant influence on nurse's performance. Incentives and organizational structures have a positive and significant influence on nurse performance. Organizational factors simultaneously positive effect on the performance of nurses in the Emergency Installation Banjarmasin Ulin General Hospital for 55.10% and the remaining 44.90% influenced by other variables. Keywords: Organization Factor, Nurse Performance, Emergency Installation.

HEMODYNAMIC STABILITY AND RECOVERY TIME OF PROPOFOL AND SEVOFLURANE IN ULIN GENERAL HOSPITAL BANJARMASIN

Abstract: The main interest in general anesthesia is safety and the well being of the patient especially the stability of hemodynamic during induction until extubation. The most common causes of prolonging awakening are residual effects of drugs either anesthetics, sedatives, and analgesics. This study aimed to compare hemodynamic stability and recovery time between propofol and sevoflurane at Ulin General Hospital Banjarmasin. This was an observational analytic study conducted in July until November 2016 on 31 patients. The result showed that mean systolic blood pressure, diastolic blood pressure and heart rate for 90 minutes maintenance were no significant difference in group-A (propofol) compare with group-B (sevoflurane), post hoc Bonferroni test showing p&gt;0.05. Mean recovery time to consciousness was faster in group-A (10,46 minutes) than group-B (15,59 minutes), there was no significant difference in two group. In conclusion, there was no significant difference in hemodynamic stability and recovery time between propofol and sevoflurane. Keywords: hemodynamic stability, recovery time, propofol, sevoflurane.

IMMATURE TO TOTAL NEUTROPHIL RATIO (I/T RATIO) ON NORMAL AND SEPSIS RISK NEONATUS FACTOR AT ULIN GENERAL HOSPITAL BANJARMASIN

Abstract: Neonatal sepsis is the main cause of morbidity and mortality in neonates. Early diagnostic and appropriate treatment can reduce the mortality and morbidity rate. Immature neutrophil ratio tand total neutrophil (I/T ratio) can be used as one of early diagnostic tool of neonatal sepsis. The purpose of this research was to find difference of I/T ratio between normal neonates and neonates with sepsis risk factor at Ulin General Hospital Banjarmasin on April – June 2016. It was an analytic observational study and collected by accidental sampling. From 39 neonates, there was 23 normal neonates and 16 neonates with sepsis risk factor who diagnosed by competence pediatrics based on mayor and minor risk factor. Mean I/T ratio from normal neonates is 0,06 and neonates with sepsis risk factor is 0,16. From statistical test with independent T-test, there is significant difference p=0,000 (p&lt;0,05). In conclusion, there is a difference of I/T Ratio between normal neonates and neonates with sepsis risk factor. Keywords: neonatal sepsis, I/T ratio, risk factor, neonates

CD4 LYMPHOCYTE IN HIV / AIDS BEFORE AND AFTER ANTIRETROVIRAL THERAPY

Abstract: Human Immunodefficiency Virus (HIV) is a virus that decreases the human immunity system, therefore the infected people become susceptible to any kind of infections. Examination of CD4 lymphocyte count periodically is one of the antiretroviral therapy success indicators. The purpose of this research was to determine the difference of CD4 lymphocyte count before and after antiretroviral therapy at Ulin General Hospital Banjarmasin on 2013-2015. The method of this research was observational analytic with cross-sectional approach. The study population was 55 patients which were selected by inclusion and exclusion criteria. Results portrayed an increase of the CD4 lymphocyte count in 51 patients after being given four kinds of antiretroviral for 6 months with the mean increase in CD4 lymphocyte count was 92,72 cell/µL. Data analysis result with Wilcoxon test portrayed a difference of the CD4 lymphocyte count before and after antiretroviral therapy with p value=0,000. In conclusion, there was a significant difference of the CD4 lymphocyte count before and after antiretroviral therapy. Keywords: Human Immunodeficiency Virus, CD4, lymphocyt, antiretroviral therapy

CORRELATION TRIGLISERIDA LEVEL AND MONOCYTE IN CORONARY HEART DISEASE AT ULIN GENERAL HOSPITAL BANJARMASIN

Abstract: Coronary heart disease (CHD) is an unbalance condition between oxygen supply and demand in heart muscle which caused by coronary artery obstruction known as atherosclerosis. Both triglyceride and monocyte have important role in the process of atherosclerosis plaque forming which cause CHD. This research aimed to analyse correlation between triglyceride level and monocyte count in patients with coronary heart disease at Ulin General Hospital Banjarmasin on August 2014-August 2015. This research is an analytical observational research with cross-sectional approach. Total sample for this research are 94 CHD patients selected by inclusion criteria. The result discovered the average rate for triglyceride level is 114 mg/dL and for monocyte count is 0,62 thousand/µL. Based on result of data analysis with Pearson correlation test demonstrates the value of r=0,347 and p=0,01. It can be concluded that there is weak, significant and positive correlation between triglyceride level and monocyte count in CHD patients Keywords: triglyceride, monocyte, coronary heart disease

Klebsiella pneumoniae IDENTIFICATION OF PRODUCING EXTENDED SPECTRUM BETA LACTAMASE (ESBL) AND RESISTANCE PATTERN OF ANTIBIOTICS IN ULIN GENERAL HOSPITAL BANJARMASIN

Abstract: Klebsiella pneumoniae(K. pneumoniae) can synthesize beta lactamase enzymes that have the ability to open lactam beta rings and cause resistance to antibiotics such as amoxicillin, third generation cephalosporins namely cefotaxim, ceftazidim, ceftriaxone and monobactam groups such as aztreonam. Extended spectrum beta lactamase (ESBL) is a plasmid enzyme that mediates the occurrence of hydrolysis and inactivation of beta lactam antibiotics. Infections caused by ESBL-producing bacteria become a serious problem for hospitals worldwide. ESBL isolate susceptibility tests against antibiotics are required to provide a sensitivity pattern that can be used as a reference in more rational antibiotic selection. This study was conducted to describe the prevalence of ESBL group K. pneumoniae and antibiotic resistance pattern at Ulin General Hospital Banjarmasin. The method of this research used secondary data material in the form of reporting document of antibiotic sensitivity test result that isolation and identification using VITEK 2, and then be analyzed using Microsoft Excel. The culture examination toward 50 patients in the Dahlia ward, Ulin General Hospital Banjarmasin was taken from January until May 2017, with the results arefrom 24 data Staphylococius aureus (S.aureus) isolate, more than half of it are ESBL group (54.16%). The results of sensitivity test of K.Pneumoniae of ESBLpositive to antibiotic group were found to be antibiotics that still have&gt; 90% sensitive were meropenem (100%), amikacin (100%), tigecycline (92,85%), ertapenem (92,86%). The antibiotics with resistance&gt; 50% was found in ampicillin antibiotics (100%), ceftriaxone (78.5%), ceftazidime (64.28%), cefepime (57.14%) and aztreonam (71.4%). As for ESBL negative results obtained sensitive&gt; 90% for all antibiotics except ampicillin, ampicillin / sulbactam, and nitrofurantoin. Meropenem (100%), amikacin (100%), tigecycline (92.85%), ertapenem (92.86%) are antibiotic choices that are still sensitive to ESBL infection Keywords: antibiotic resistance, ESBL pattern, Vitek 2

A New Insight of Effect of Ampicillin-Gentamicin Through Chlorinative Stress Mechanism in Saliva of Newborn at Risk of Sepsis

Clinical diagnosis of sepsis in neonate is difficult, because many signs of sepsis are nonspecific. There are several salivary biomarkers of stress as objective indicators of stress reactions.This study was designed to investigate the effect of ampicillin-gentamicin treatment to chlorinative stress parameters insaliva of newborn at risk of sepsis. Four chlorinative stress parameters were used, hydrogen peroxide (H2O2) levels, myeloperoxidase (MPO) activity, advanced oxidation protein products (AOPPs) levels, and chlorinative index (CI). This study was performed in May until September 2016. Saliva samples were taken from 26 newborns at risk of sepsis treated in Ulin General Hospital, Banjarmasin, South Kalimantan, Indonesia. All newborns were given ampicillin-gentamicin three until ten days. The H2O2 levels, MPO activity, AOPPs levels, and CI were measured from the saliva taken from both pre and post treatment. Statistical analysis of the parameters were obtained from before and after the ampicillin-gentamicin treatment, using Mann-Whitney test. The used ofampicillin-gentamicin for treatment of newborns at risk of sepsis showed significant decrease in the H2O2 levels, MPO acitivity, AOPPs levels, and significant increases in the CI. From this results, it can be concluded that Ampicillin-gentamicin could reduce the chlorinative stress in newborn at risk of sepsis

LAYANAN PESAN SINGKAT PENGINGAT MENINGKATKAN KEPATUHAN MINUM OBATPASIEN DIABETES MELITUS DI RSUD ULIN BANJARMASIN

Diabetes melitus is one ofthe metabolic disorders with characteristic hyperglycemia that occurs due to abnormal insulin secretion, insulin resistance or both. The non adherence patients of taking antidiabetic drugs are the main factors that could cause high blood glucose levels, so it is necessary an intervention to achievedoutcome therapy desired. Giving of short message service reminder intervention in diabetes mellitus patients was expected to improved the medication adherence and achieved normal blood glucose levels.This study was conducted to determine the effect of a short message service reminder on medication adherence of ambulatory diabetes melitus patients in Ulin General Hospital Banjarmasin.This study was conducted with quasi-experimental design,the data were taken prospectively during May to June, 2014. The subjects were ambulatory diabetes melitus patients in Ulin General Hospital Banjarmasin who had received oral antidiabetic drugs. Subject who met the inclusion and exclusion criteria were 39 patients and had given an intervention for seven days. The data collected by interviews and pill counting on filling sheet. The blood glucose levels data was taken from their medical records.The result showed that giving of a short message service reminder intervention improve patient adherence (p&lt;0,05). Fasting blood glucose level and blood glucose level two hours post prandial have decreased significantly (p&lt;0,05). There were correlation between the patient adherence and the decreasing in fasting blood glucose levels (p=0,050; r=0,316) and blood glucose two hours post prandial levels (p=0,010; r=0,040).Based on these result, it can be concluded that the giving of short message service reminder in diabetes melitus patientshas been improved patient adherence.

Occurrence of measles genotype D8 during a 2014 outbreak in Banjarmasin, South Kalimantan, Indonesia

An outbreak of measles symptoms occurring in children in Banjarmasin, South Kalimantan, Indonesia in 2014 was investigated. Nasal swabs were collected from 23 children (median age 41 months) with fever and other symptoms of measles hospitalized in Ulin General Hospital and Islamic Hospital, Banjarmasin, South Kalimantan. Viral RNA was extracted for cDNA synthesis, followed by PCR and sequencing using paramyxovirus family consensus and N-gene primers. Sixteen measles-positive patients (70%) were identified. Fifteen virus strains belonged to genotype D8 and the remaining one strain was confirmed as belonging to genotype D9. Measles virus genotype D8 was detected in an outbreak of measles in South Kalimantan, Indonesia, in 2014.

Neutrophil, TLR2, and TLR4 expression in newborns at risk of sepsis

Background There is increasing evidence that toll-like receptors (TLR) play a key role in the mediation of systemic responses to invading pathogens during sepsis. Saliva is an important body fluid for detecting physiological and pathological conditions of the human body. Neutrophils are participants in the acute response against pathogens in many tissues, and their influx into the oral cavity may occur at any time.Objective To compare mean neutrophils and the expression of TLR2 and TLR4 in saliva and blood of newborns at risk for sepsis to those of healthy newborns.Methods This cross-sectional study was conducted from July to December 2011 in the Division of Neonatology, Department of Child Health, Ulin General Hospital, Lambung Mangkurat University Medical School, Banjarmasin. Case subjects were newborns with sepsis risk factors (30 infants), while 30 healthy infants were in the control group. Saliva and blood specimen examinations were performed in the Biomedical Laboratory of Brawijaya University Medical School, Malang. We used T-test for statistical analyses.Results From saliva specimens, mean neutrophils were significantly higher in the case group than in the control group [14.43 (SD 12.21) % vs. 5.63 (SD 6.78) %, respectively, (P=0.021)]. In addition, mean TLR2 and mean TLR4 saliva levels were significantly higher in the case group than in the control group [TLR2: 64.97 (SD 26.42) % vs. 40.06 (SD 6.23) %, respectively, (P=0.011); TLR4: 1.5 (SD 1.61) % vs. 0.57 (SD 0.53) %, respectively, (P=0.044)]. From blood specimens, mean neutrophils were also significantly higher in the case group than in the control group [1.09 (SD 0.61)% vs. 0.21 (SD 0.09)%, respectively, (P=0.000)]. Similarly, mean blood TLR2 and TLR4 levels were significantly higher in the case group than in the control group [TLR2: 92.51 (SD 5.51) % vs. 81.74 (SD 11.79) %, respectively, (P=0.003); TLR4: 0.71 (SD 1.42) % vs. 0.12 (SD 0.06) %, respectively, (P=0.000)].Conclusion There are significant increases in neutrophils, as well as neutrophil expression of TLR2 and TLR4 in the saliva and blood from newborns with sepsis risk factors compared to those of healthy newborns. [Paediatr Indones. 2013;53:132-7.]

PARP-1 expression against Epstein-Barr virus LMP-1 and BZLF-1 in undifferentiated nasopharyngeal carcinoma

Objective: This study aims to determine the poly(ADP-ribose) polymerase (PARP)-1 expression levels against the Epstein-Barr virus (EBV) latent membrane protein (LMP-1) and BamHI-Z leftward reading frame (BZLF)-1 of nasopharynx tissues and serum of nasopharyngeal carcinoma (NPC) patients and characterize the histopathology of necrosis cells of NPC tissues. Methods: 32 tissues sections consisting 24 NPC and 12 polyp tissues, and also blood serum of patients were taken from Ulin General Hospital, Banjarmasin. Tissue samples were analyzed by using immunofluorescence staining in order to examine LMP-1 and PARP-1 expression. Analyses were done by using immunoblotting with anti-LMP-1, anti-BZLF-1, anti-PARP-1, and anticaspase-3 antibodies. Histopathological profiles of necrosis cells were analyzed by using hematoxylin-eosin staining and necrotic cells were counted by using the standard software of the microscope. Results: LMP-1, BZLF-1 and PARP-1 proteins have high levels of expression in patients with NPC. LMP-1 expression of NPC patients and control sera are equal. Interestingly we found that 35 kDa of BZLF-1 and 55 kDa PARP-1 only expressed in NPC patients, meanwhile caspase-3 expression was negative. Histopathologically, NPC tissues were more dominated by karyorrhexis than pyknosis and karyolysis. Conclusions: This study showed that the expression level of PARP-1 in NPC tissues was increased against LMP-1 and BZLF-1 EBV to protect DNA damage of the recipient cells. This mechanism probably stimulated cell metamorphosis and consequently provoked necrosis. © 2013 GESDAV