Total Antioxidant Capacity Assay Research Articles

Quality evaluation of Eucommiae Folium extracts and discovery of efficacy-associated quality markers based on spectrum-effect relationship

ABSTRACT The spectrum-effect relationship between chemical fingerprints and antioxidant activities was established to evaluate the quality of Eucommiae Folium (EF) extracts and discover potential efficacy-associated quality markers. Characteristic fingerprints of different batches of EF extracts were generated by high-performance liquid chromatography (HPLC), and a similarity analysis was performed by hierarchical cluster analysis (HCA). Then the total antioxidant capacity assay kit with ferric reducing antioxidant assay (FRAP) method was used to determine antioxidant activity. The spectrum-effect relationship was established to identify the chemical constituents that impart antioxidant activities of Eucommiae Folium (EF) extracts using partial least squares regression (PLSR). HPLC fingerprints of EF extracts were established with the optimized chromatographic conditions: the mobile phase consisted 0.1% phosphoric acid -water (A) and acetonitrile (B), the detection wavelength was 326 nm, the column temperature was 30°C, and the flow rate was 0.8 mL/min. The correlation coefficient of similarity between 12 batches of extracts was ≥0.995. Compounds corresponding peaks P1, P2, P6 (chlorogenic acid), and P8 were closely related to the stable antioxidant activities of EF extracts, and could be selected as potential quality markers. An agile and efficient method that can ensure the intra-assay stability of biological activity of EF extracts and discover efficacy-associated quality markers was successfully built based on spectrum-effect relationship.

Naringenin mitigates cadmium-induced cell death, oxidative stress, mitochondrial dysfunction, and inflammation in KGN cells by regulating the expression of sirtuin-1

The objective of this study was to examine the potential protective role of naringenin against the harmful effects induced by cadmium in KGN cell line. Cell viability was evaluated by cell counting kit-8 assay. Caspase-3/-9 activities were determined by caspase-3/-9 activity assay kits, respectively. Intracellular reactive oxygen species (ROS) level was detected by ROS-Glo™ H2O2 Assay, antioxidant capacity was determined by a total antioxidant capacity assay kit. Mitochondrial membrane potential (MMP), ATP level, and ATP synthase activity were determined by JC-1, ATP assay kit, and ATP synthase activity assay kit, respectively. The mRNA expression was determined by qRT-PCR. Cadmium reduced cell viability and increased caspase-3/-9 activities in a concentration-dependent manner. Naringenin improved cell viability and reduced caspase-3/-9 activities in cadmium-stimulated KGN cells in a concentration-dependent manner. Cadmium diminished the antioxidant capacity, increased ROS production, and induced mitochondrial dysfunction in KGN cells. These effects were ameliorated by naringenin treatment in a concentration-dependent manner. Furthermore, naringenin reduced the levels of pro-inflammatory cytokines in KGN cells exposed to cadmium. SIRT1 knockdown downregulated its expression in KGN cells and compromised the protective effects of naringenin on cell viability and caspase-3/-9 activities in cadmium-stimulated KGN cells. Naringenin prevented the reduction of MMP, ATP levels, and ATP synthase activity in cadmium-stimulated KGN cells in a concentration-dependent manner. However, these protective effects were significantly reversed by SIRT1 knockdown. In conclusion, this study suggests that naringenin protects against cadmium-induced damage by regulating oxidative stress, mitochondrial function, and inflammation in KGN cells, with SIRT1 playing a potential mediating role.

Therapeutic effects of Salvia balansae on metabolic disorders and testicular dysfunction mediated by a high-fat diet in Wistar rats

Medicinal plants offer an important therapeutic resource in treatment of male infertility. We aim to evaluate the possible therapeutic effects of Salvia balansae on metabolic disorders and testicular dysfunction resulting from a high-fat diet (HFD). Antioxidant activity of aqueous extract of S. balansae leaves was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay and total antioxidant capacity (TAC) assay. Antidiabetic activity was determined by α-amylase inhibition. In vivo, HFD was administered in Wistar rats for 18 weeks and aqueous extract of S. balansae for the last 6 weeks (200 mg/Kg of body weight/day). At the term of experimentation, testosterone and some plasma parameters were analyzed and removed testes were subjected to a histomorphometric study. Our results show high levels of phenolic components in aqueous extract of S. balansae and significant antioxidant and antidiabetic activity. HFD increases body weight, causes type 2 diabetes, dyslipidemia, liver failure and inflammation. Also, HFD decreases testosterone and alters testis histological structure (seminiferous tubular degeneration, impaired spermatogenesis and interstitial fibrosis). Treatment of HFD rats with extract of S. balansae normalizes body weight and plasma parameters, increases testosterone and regenerates testicular structure and function. In summary, S. balansae could reduce metabolic complications induced by HFD and serve the basis for developing a new therapy for testicular dysfunction.

Comparative study of total reducing and total antioxidant capacity of Escherichia coli on different media composition obtained by response surface methodology

Anti-oxidants are compounds that act against oxidative stress, prevent DNA damage and lipid peroxidation which are usually the major causes of aging and many diseases including cancer, cardio vascular disorders etc. There are many anti-oxidant assays which are used to measure anti-oxidant potential but total anti-oxidant capacity (TAC) and total reductive capability (TRC) are more preferred because of ease to use, good reproducibility and accuracy. Media components were optimized through response surface methodology (RSM) and sixteen different media compositions were obtained at primary level. These media compositions were used for TAC and TRC antioxidant assays. The highest reducing power activity was observed in the form of absorbance in media number 4 (0.0275 for 10 min) in wet biomass and for media number 6 (0.0244 for 10 min) in dry biomass. After 30 min, highest reducing power was reported in media number 5 (0.0286) in wet biomass and for dry biomass it was in media number 6 (0.0230). Total anti-oxidant capacity showed the higher percentage inhibition of microbial growth for wet biomass in media number 4 and media number 13 and for dry biomass, the highest percentage inhibition was in media number 5. Therefore, it was observed that media have same nutrients but different compositions which directly affect the level of TAC and TRC. Hence it is an important parameter that before experiments, media components must be optimized by RSM.

Anticancer effect of antioxidant-rich methanolic extract of Rauvolfia serpentina (L.) Benth. ex Kurz leaves in HepG2 and HeLa cells: A mechanistic insight

Rauvolfia serpentina (L.) Benth. ex Kurz is a pharmaceutically, and agriculturally important medicinal plant traditionally used against snake and insect bites, dysentery, abdominal pain, fever, and insanity. However, no detailed study has been done on anticancer potency of crude leaf extract of Rauvolfia serpentina. Therefore, we investigated the anticancer activity of methanolic extract of Rauvolfia serpentina leaf (MERSL) in human hepatocarcinoma HepG2 and cervical cancer HeLa cells along with its antioxidant capacity. GC-MS analysis revealed the presence of many anticancer phytochemicals including campesterol, stigmasterol, beta-sitosterol, alpha-amyrin, lupeol, phytol, and D-pinitol. The antioxidant activity of MERSL was confirmed by DPPH (IC50 = 106.16 μg/ml), ABTS (IC50 = 146.67 μg/ml), FRAP (IC50 = 1.4 mg/ml), and total antioxidant capacity assay invitro, and reduction of H2O2-induced ROS in noncancer fibroblast F111 cells. Crystal violet assay showed that MERSL reduced cell viability in HepG2 (IC50 = 274.12 μg/ml) and HeLa (IC50 = 231.33 μg/ml) at 24 h, but not in F111 cells at these IC50 concentrations. Further, wound healing and clonogenic assays confirmed anticancer activity, which resulted in decreased cell migration and revival capacity in HepG2 and HeLa cells. Apoptotic changes were visualized by acridine orange-ethidium bromide and DAPI staining using fluorescence and confocal microscopy. Also, comet assay indicated DNA damage. An increase in intracellular ROS, calcium influx, reduction of mitochondrial mass and membrane potential, and cell cycle arrest, established by flow cytometry and fluorimetry, highlighted the possible underlying mechanism of cell death by MERSL in HepG2 and HeLa cells. Therefore, this study evidenced that Rauvolfia serpentina leaves could be an encouraging pharmacological application for developing anticancer strategies.

A Study of Polyphenolic Compounds and in vitro Antioxidant Activity of Trianthema portulacastrum Linn. Extracts

Background: Trianthema portulacastrum Linn. is an exotic plant growing as weed in Africa, Southeast Asia and tropical America. Chemical fingerprinting under the present study was undertaken to characterize the polyphenolic compounds and antioxidant activities present in the plant. Methods: Several in vitro antioxidant assays were performed to evaluate the antioxidant activities in aqueous, alcoholic and hydro-alcoholic extracts. Characterization of polyphenolic compounds was done by HPLC and GCMS analyses. Result: Total phenols, flavonoids and total antioxidant activity as assessed by DPPH, ABTS and total antioxidant capacity assays was found to be maximum in hydroethanolic extract of the plant. HPLC analysis confirmed the presence of gallic acid, protocatechuic acid, catechin hydrate, vanillic acid, epicatechin, p-coumaric acid, rutin, salicylic acid, myricetin, quercetin and trans-cinnamic acid in plant extracts being maximum in hydroethanolic followed by aqueous, hydromethanolic, ethanolic and then methanolic extracts. Catechin, epicatechin, rutin, myricetin and quercetin were main flavonoid compounds in most of the plant extracts. GCMS analysis also confirmed the presence of several other antioxidants. Based on the findings of the phytochemical analyses and in vitro antioxidative studies, it can be clearly established that Trianthema portulacastrum has rich amounts of potent polyphenolic and flavonoid compounds having antioxidant properties.

New Specific α-Glucosidase Inhibitor Flavonoid from Thymelaea tartonraira Leaves: Structure Elucidation, Biological and Molecular Docking Studies.

The phytochemical investigation of Thymelaea tartonraira leaves led to the isolation and characterization of six compounds, including one new flavonoid glycoside identified as hypolaetin 8-O-β-D-galactopyranoside (4) along with five known compounds, daphnoretin (1), triumbelletin (2), genkwanin (3), tiliroside (5) and yuankanin (6). Their structures were established based on spectroscopic methods, such as UV, IR, NMR, and HR-ESI-MS. Triumbelletin (2) and tiliroside (5) were isolated for the first time from T. tartonraira leaves. The antioxidant property of all isolated compounds was tested based on DPPH, FRAP and total antioxidant capacity assays. Compound 4 displayed an antioxidant potency more interesting than vitamin C with an IC50 =15.00±0.50 μg/ml, followed by compound 5. Furthermore, the both compounds 4 and 5 were tested for their α-amylase inhibitory activity in-vitro. Compound 4 displayed higher potency to inhibit α-amylase, with an IC50 =46.49±2.32 μg/ml, than compound 5, with an IC50 =184.2±9.2 μg/ml, while the reference compound acarbose presented the highest potency to inhibit α-amylase with an IC50 =0.44±0.022 μg/ml. Compound 4 displayed a strong inhibitory ability of α-glucosidase activity approximately twice more than the reference compound, acarbose, with IC50 values of 60.00±3.00 and 125.00±6.25 μg/ml, respectively. Thus, compound 4 exhibited a specific inhibitory activity for α-glucosidase. The molecular docking studies have supported our findings and suggested that compound 4 has been involved in various binding interactions within the active site of both enzymes α-amylase and α-glucosidase.

Nitrogen-Doped Carbon Nanoflowers Decorated with PtNi Nanoparticles for Colorimetric Detection of Total Antioxidant Capacity

A total antioxidant capacity (TAC) assay plays an essential role in evaluating antioxidant behaviors. It mainly involves examining the dosages of antioxidants such as ascorbic acid (AA), while TAC can be measured by nanozyme with peroxidase-like activity. Herein, nitrogen-doped carbon nanoflowers decorated with uniform PtNi nanoparticles (PtNi/NCFs) were fabricated by a simple pyrolysis strategy. The obtained PtNi/NCFs showed high peroxidase-mimicking activity to catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation by generating rich hydroxyl radicals (·OH) in the H2O2 system. By virtue of the PtNi/NCFs nanozyme, a visual colorimetric sensor was developed for assays of H2O2 and AA. The limits of detection were as low as 45.3 and 0.94 μM for determination of H2O2 and AA with linear ranges of 100–1000 and 1.0–20.0 μM, respectively. Moreover, such sensor was also successfully employed to detect AA and H2O2 in real tablets and juices. The PtNi/NCFs nanozyme holds great potential for practical applications.

Essential Oils and Biological Activities of Eucalyptus falcata, E. sideroxylon and E. citriodora Growing in Tunisia

Many plants are able to synthesize essential oils (EOs), which play key roles in defense against weeds, fungi and pests. This study aims to analyze the chemical composition and to highlight the antioxidant, antimicrobial and phytotoxic properties of the EOs from Eucalyptus falcata, E. sideroxylon and E. citriodora growing in Tunisia. EOs were analyzed by gas chromatography coupled to mass spectrometry (GC/MS) and their antioxidant properties were determined by total antioxidant capacity (TAC), DPPH and ABTS assays. The phytotoxic potential was assessed against weeds (Sinapis arvensis, Phalaris canariensis) and durum wheat crop (Triticum durum) and compared to chemical herbicide glyphosate. The antifungal activity was investigated in vitro against eight target fungal strains. All EOs displayed a specific richness in oxygenated monoterpenes (51.3-90%) and oxygenated sesquiterpenes (4.8-29.4%), and 1,8-cineole, citronellal, citronellol, trans-pinocarveol, globulol, spathulenol and citronellyl acetate were the main constituents. Eucalyptus EOs exhibited remarkable antioxidant activity and E. citriodora oil exhibited significant activity when compared with E. falcata and E. sideroxylon EOs. The phytotoxic potential of the tested oils had different efficacy on seed germination and the growth of seedlings and varied among tested herbs and their chemical composition variability. Their effectiveness was better than that of glyphosate. At the post-emergence stage, symptoms of chlorosis and necrosis were observed. Furthermore, a decrease in chlorophyll and relative water content, electrolyte leakage and high levels of MDA and proline were indicators of the oxidative effects of EOs and their effectiveness as bioherbicides. Moreover, all the EOs exhibited moderate fungitoxic properties against all the tested fungal strains. Therefore, according to the obtained results, Eucalyptus EOs could have potential application as natural pesticides.

CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITIES OF TANNINS EXTRACT FROM ALOE SAPONARIA

The objective of this work was to study the chemical composition and antioxidant activity of tannins extract of Aloe saponaria (TEAS). The Aloe saponaria (syn. Aloe maculata) is a succulent plant belonging to the Xanthorrhoeaceae family, this succulent plant native to South Africa was introduced in Morocco as an ornamental plant, rustic and easy to grow. In order to ensure this, morphological and histological study method of the species, phytochemical screening, characterization of TEAS by gas chromatography coupled to mass spectrometry (GC/MS), and the study of antioxidant activity by two methods (2,2-diphenyl-1-picrylhydrazyl (DPPH) and total antioxidant capacity (TAC) assays) were examined. The results of phytochemical screening indicated that the Aloe saponaria leaf was rich of alkaloids, tannins catechics, flavonoids, sterols, triterpenes, oses, holosides, mucilages and reducing sugars. In addition, the main constituents of TEAS identified by GC/MS analysis were Linolenic acid (32.22%), Palmitic acid (17.42%), and Phytol (11.30%). Regarding antioxidant activity, the TEAS showed a significant antioxidant effect.

Leaf-buds of Pistacia atlantica: a novel source of bioactive molecules with high anti-inflammatory, antioxidant, anti-tyrosinase and antimicrobial properties.

In this study, for the first time, the anti-inflammatory, antioxidant, anti-tyrosinase and antimicrobial property of P. atlantica Desf. subsp. atlantica leaf-bud extracthave been investigated. The anti-inflammatory activity was determined in vivo by reducing carrageenan-induced hind paw edema in mice, while the antiradical function was evaluated using DPPH, total antioxidant capacity (TAC) and reduction power assays. The extract induced a significant reduction of the edema, from 1 to 6h in a dose-dependent manner (150, 200 and 300mg/kg). Histological observations of the inflamed tissues also confirmed this. An effective antioxidant activity of the plant samples was demonstrated, showing an EC50 = 0.183 ± 0.005mg/mL for the DPPH test, a value of 28.776 ± 2.541mg AAE/g for the TAC and an EC50 = 0.136 ± 0.003mg/mL for reducing power. The leaf-bud extract also revealed a good antimicrobial activity against S. aureus and L. monocytogenes (mean diameter of inhibition zones of 13.2 and 17.0mm, respectively), while a slight antifungal effect was observed. The plant preparation was then documented to inhibit tyrosinase activity, with an EC50 value of 0.098 ± 0.00mg/mL in a dose-dependent manner. HPLC-DAD analysis revealed that dimethyl-allyl caffeic acid and rutin were the most abundant molecules. The current data documented that P. atlantica leaf-bud extract has strong biological properties and constitutes a potential source of pharmacological molecules.

Effects of medwakh smoking on salivary metabolomics and its association with altered oral redox homeostasis among youth

The use of alternative tobacco products, particularly medwakh, has expanded among youth in the Middle East and around the world. The present study is conducted to investigate the biochemical and pathophysiological changes caused by medwakh smoking, and to examine the salivary metabolomics profile of medwakh smokers. Saliva samples were collected from 30 non-smokers and 30 medwakh smokers and subjected to metabolomic analysis by UHPLC-ESI-QTOF-MS. The CRP and Glutathione Peroxidase 1 activity levels in the study samples were quantified by ELISA and the total antioxidant capacity (TAC) by TAC assay kits. Statistical measurements and thorough validation of data obtained from untargeted metabolomics identified 37 uniquely and differentially abundant metabolites in saliva of medwakh smokers. The levels of phthalate, L-sorbose, cytosine, uridine, alpha-hydroxy hippurate, and L-nicotine were noticeably high in medwakh smokers. Likewise, 20 metabolic pathways were differentially altered in medwakh smokers. This study identified a distinctive saliva metabolomics profile in medwakh smokers associated with altered redox homeostasis, metabolic pathways, antioxidant system, and CRP levels. The impact of the altered metabolites in medwakh smokers and their diagnostic utility require further research in large cohorts.

Antioxidant, cytotoxic, antibacterial and thrombolytic activities of Centella asiatica L.: possible role of phenolics and flavonoids

BackgroundCentella asiatica L. (Apiaceae), a green leafy vegetable, has been used as a popular folk medicine in Bangladesh in the treatment of various ailments. The plant is reported to contain phenolics and flavonoids, but their bioactive potentials are not yet completely known. The present study was designed to investigate the role of the phenolic and flavonoids in the antioxidant, cytotoxicity, antibacterial and thrombolytic activities.MethodsFour solvent fractions viz. methanol (MSF), ethyl acetate (ESF), chloroform (CSF) and petroleum ether (PSF) were prepared from the dried powder of the whole plant by the modified Kupchan method. Total phenolic content and flavonoid content were determined by Folin Ciocalteu method and aluminum chloride colorimetric method, respectively. The antioxidant activity was assessed by the DPPH radical scavenging and total antioxidant capacity assays. The antibacterial activity was determined by the disc diffusion method and cytotoxicity was evaluated by the brine shrimp lethality bioassay. Thrombolytic activity was assayed using streptokinase as standard.ResultsQualitative analysis of phytochemical revealed the presence of phenolics and flavonoids along with other bioactive constituents. Among the extractives, CSF contained the highest content of phenolics (155.46 ± 0.52 mg GAE/g) and flavonoids (345.17 ± 1.12 mg QE/g) and exhibited the most potent antioxidant activity in terms of total antioxidant capacity (179.01 ± 0.89 mg AAE/g) and DPPH scavenging ability (IC50; 15.31 ± 0.32 µg/mL). Similarly, CSF showed the highest cytotoxicity with LC50 values of 13.80 ± 0.23 µg/mL, and thrombolytic activity with 43.94 ± 0.62% clot lysis. The fraction also exhibited broad spectrum antibacterial activity. A significant correlation was observed between the flavonoid content and total antioxidant activity (r2 = 0.894, p < 0.05), while high correlation was seen between phenolic and flavonoid content and DPPH radical scavenging, total antioxidant capacity and cytotoxicity (r2 = 0.612–0.928). Similarly, a positive correlation was found between phenolic and flavonoid content with thrombolytic and antibacterial activities.ConclusionThese results revealed that C. asiatica is a rich source of phenolics and flavonoids and correlated with antioxidant, cytotoxicity, antibacterial and thrombolytic activities. Hence isolation of phenolics and flavonoids from this plant may offer potential candidates which may be effective in the prevention of many chronic diseases.

Phytochemical composition and potential anti-inflammatory and antioxidant mechanisms of leaf extracts of Sida linifolia L. (Malvaceae)

IntroductionThe classes of phytocompounds and in vitro anti-inflammatory and antioxidant potentials of crude aqueous extract (CAE) and ethanol extract (CEE) of Sida linifolia L. leaves were studied. MethodsThe assayed in vitro anti-inflammatory parameters were phospholipase A2 activity, platelet aggregation, albumin denaturation, membrane stabilization, protease inhibition, and heat-induced membrane hemolysis using the human red blood cells (HRBCs) method. Also, antioxidant properties of extracts were determined using reducing power assay, nitric oxide assay, total antioxidant capacity assay, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The anti-inflammatory study used aspirin and prednisolone as reference drugs, while the antioxidant assay deployed butylated hydroxytoluene (BHT), gallic acid, and ascorbic acid as standard drugs. All parameters were determined following conventional procedures. Results and discussionPhytochemical screening of both extracts showed varying amounts of tannins, saponins, flavonoids, steroids, cyanogenic compounds, glycoside, alkaloids, phenolics, and terpenoids. At various concentrations (0.2–1.0 mg/ml), the CAE and CEE exhibited significantly (P < 0.05) high % anti-inflammatory activities similar to the standard drugs (aspirin/prednisolone), and these correlated positively with concentration. However, CAE exhibited better anti-inflammatory activities than CEE across all the parameters assayed. The in vitro antioxidant study revealed that both extracts demonstrated good antioxidant activities, which increased with concentrations. The ranges of IC50 values (in mg/ml) for CAE (0.64–0.92) and CEE (0.51–0.62) showed that CEE possesses more antioxidant potential compared with CAE; however, both extracts were less potent compared with gallic acid (0.47), ascorbic acid (0.32–0.50), and BHT (0.30). ConclusionsThis study suggests that Sida linifolia leaf extracts are rich in some classes of biologically active phytocompounds and exhibit good anti-inflammatory and antioxidant activities.

Antioxidant Phenolics of Justicia adhatoda L. and Cordia dichotoma Frost. Promote Thrombolytic Activity through Binding to a Serine Protease, Tissue Plasminogen Activator Protein

Abstract Background: The tissue plasminogen activator (tPA) protein dissolutes fibrin clots and prevents the disease like thrombosis. The current study aimed to study the tPA-promoting activity of bioactive molecules of Justicia adhatoda L (JA) and Cordia dichotoma Frost (CD). Methods: The phytochemical characterization of methanolic and aqueous extracts of JA and CD stems was performed through qualitative analysis, Fourier-transform infrared spectroscopy (FTIR), and biochemical tests (total phenolic and total flavonoid content [TPC and TFC]). The bioactivity of the extracts was studied through total antioxidant capacity (TAC) and ferric-reducing antioxidant potential (FRAP) assays. Finally, forty phytocompounds from JA and CD were identified from the literature, and in silico molecular docking study was performed to target tPA protein (PDB id 1A5H, Chain A, X-ray diffraction, resolution 2.90 Å). Results: Various phytochemical classes were identified from extracts, through qualitative and FTIR analysis. TPC and TFC were estimated from the JA and CD extracts within the range of 9.34–28.67 mg gallic acid equivalent/100 g of extract weight (EW) and 2.48–16.17 mg quercetin equivalent/100 g of EW, respectively. The aqueous extract of CD showed the highest TAC of 14.90 ascorbic acid equivalent (AAE)/100 g of EW, and the methanolic extract of JA had the highest FRAP activity of 27.77 mg AAE/100 g EW. The molecular docking study showed that apigenin 6,8-di-glucopyranoside had the highest binding potential toward the tPA (−9.380 kcal/mol). Conclusion: It can be concluded that antioxidant phytochemicals of JA and CD could promote the tPA activity, thereby promoting thrombolytic activity.

Screening and identification of antioxidant active ingredients from red wine via spectrum-effect relationship analysis

Red wine was a prominent constituent of diets across the globe and modern pharmacological studies showed that red wine had the main function of antioxidant. However, the reported screening methods for active ingredients were inefficient and insensitive. In this research, 35 batches of red wine were used to establish the chromatography fingerprint, which were further utilized to screen and identify the antioxidant active ingredients through spectrum-effect relationship analysis. Indeed, the antioxidant capacity of red wine was evaluated by total antioxidant capacity assay kit (ABTS method). After evaluating the antioxidant capacity of samples, correlations were analyzed by Pearson correlation analysis, Spearman correlation analysis, and Gray Relational Analysis (GRA). Moreover, spectrum-effect relationships combined with ultrafast liquid chromatography/quadrupole-time-of-flight mass spectrometry (UFLC-Q-TOF/MS) revealed that the succinic acid, gallic acid, protocatechuic acid, catechin, syringic acid, salicylic acid, homogentisic acid, ellagic acid, isorhamnetin-3-O-glucoside, and trans- resveratrol show antioxidative efficacy. Collectively, the present study established the spectrum-effect relationship strategy of red wine and identified active antioxidant ingredients, which would give reasonable index components for the health function of red wine and provide reference for quick screening of bioactive compounds for food.

Determination of physical, chemical and antioxidant properties of pomegranate sauces sold in Turkish markets

In this study, it was aimed to determine the physical, chemical and antioxidant properties of pomegranate sauces sold in the market in Turkey. A total of 18 pomegranate sauces were studied; 17 of which were purchased from the market, and one of them was produced in a rotary evaporator in the laboratory. For each sample, analysis of antioxidant activity, titratable acidity, brix, hydroxymethyl furfural, pH, color analysis, and sugar analyzes were performed. Antioxidant activity values were analyzed using 6 different methods (DPPH (2,2-diphenyl-1-picrylhydrazil) radical scavenging activity, ferric reducing antioxidant power (FRAP), ABTS●+ radical scavenging capacity, total antioxidant capacity assay (TAC), the total phenolic content (TPC) and total flavonoid content (TFC) methods). The lowest and highest values were determined for methods of DPPH (5.23- 2822.69 mg AA/kg), FRAP (57.94 - 2380.94 mg FeSO4/kg), TAC (660.47 - 3690.83 mg AA/kg), TFC (23.06 - 11680.71 mg QEE/kg), TPC (123.54 -9566.95 mg GAE/kg). Brix, pH and % titration acidity values varied between 70.00 - 76.70%, 1.66 -2.88, 2.65 -7.58%, respectively. In addition, HMF values were measured below the limit values in most of the samples, while these values were found to be high in some of the samples. The highest and lowest HMF values were measured between 4.58 -103.68 mg/kg, respectively. Many factors such as raw materials, additives, applied heat treatments, production processes and storage conditions are effective on produced pomegranate sauces. If the production conditions are regulated in accordance with the standards, the HMF values in pomegranate sauce will be below the limit.

Gold nanoparticle-decorated MoSe2 nanosheets as highly effective peroxidase-like nanozymes for total antioxidant capacity assay

Gold nanoparticle-decorated MoSe2 nanosheets as highly effective peroxidase-like nanozymes for total antioxidant capacity assay

Effect of Mature Stages on Phenolic Contents, Antioxidant Activities and Mineral Distribution in Different Parts of Kei Apple Fruit

This study aimed to evaluate the concentrations of the heavy metals, macro and micro-elements as well as the distribution of phenolic constituents in different parts of half-ripe and full-ripe kei apple fruits. For mineral analysis, fruit samples were digested and analyzed using inductively coupled plasma optical emission spectrometry (ICPOES). The colorimetric determination of total polyphenolic contents (TPs) and the antioxidant capacity using DPPH and total antioxidant capacity (TAC) assays were done using aqueous alcoholic extracts. Also, the phenolic profiles were identified and quantified using high performance liquid chromatography (HPLC). The results showed that the highest antioxidant activity using DPPH and TAC and total phenolic content were detected in the pulp of full-mature fruits and its values were 2.03 mg GAE/g d.w, 22.61 mg AAE/g d.w and 26.24 mg GAE/g d.w, respectively. Average of mineral concentrations in dried samples (in μg/g), were ranged between: 6,164.99 to 18,339.61 (K); 2,538.06 to 3,488.92 (Ca); 749.26 to 2,110.12 (Mg); 82.13 to 733.36 (Fe); 22.74 to 58.02 (Zn); 6.43 to 11.85 (Mn); 10.17 to 20.02 (Cu); 1.86 to 8.73 (Ni) and much lower concentrations of Co, Pb and Cd. The key phenolic constituents of the fruit harvested in different times were 4-hydroxy benzoic acid, chlorogenic acid and catechol. Overall, harvesting of kei apple fruit in the first week of June indicates the full maturity of the fruit with characteristic higher phenolics, antioxidant capacity without any deterioration in most of elemental minerals.

Black Rice Extract Induced Apoptosis and Cell Cycle Arrest on Human Cancer Cell Lines

The purpose of the research is to evaluate the anti-cancer outcomes of aqueous black rice (BR) extract on human cancer cell lines; HeLa, T47D, and U2OS. To begin the investigation, the total phenol and flavonoid content of the BR extract was determined. Additionally, the antioxidant activity of the extract was determined using the ferric reducing antioxidant power (FRAP), total antioxidant capacity (TAC), and 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) assays. The cell viability, apoptosis and cell cycle on several human cancer cells were determined after BR extract administration in several doses (BR1; 100 mg/ml, BR2; 200 mg/ml, BR3; 300 mg/ml, BR4; 400 mg/ml, and BR5; 500 mg/ml) using flow cytometry analysis. According to the analysis, the extract had total phenol and flavonoid content are 66.42 mg/g and 11.12 mg/g, respectively. Based on the assay of DPPH (53.19 µg/ml), FRAP (49.86 mg/g), and TAC (96.70 mg/g), BR extract showed strong antioxidant activity. This study also revealed that BR extract significantly reduced human cancer cell lines viability and selectively (shown to have no effect on PBMC cells) (p&lt;0.05) especially BR5 group (500 g/mL) . The administration of BR extract was most responsive to induce apoptosis in T47D cells, up to 93.64 % in BR5 close to cisplatin-induced apoptosis effect, compared to HeLa and U2OS cells. The study also found BR extract induced the cell (HeLa, T47D, and U2OS) to arrest or in the phase G0/G1. Hence, BR extract was a food-based cancer treatment with a promising effect to inhibit cancer cell growth and progression.