Tissue Culture Laboratory Research Articles

Radiosensitivitas Kalus Embriogenik Kopi Robusta Bp 436 Terhadap Iradiasi Sinar Gamma

<p><em>High genetic diversity is one factor that determines the success of plant breeding. Mutation induction by gamma ray irradiation is one method to improve plant genetic diversity. This study aimed to 1) obtain growth regulators composition suitable in inducing embryogenic callus, 2) determine the effect of gamma ray irradiation on the growth and development of somatic embryos, and 3) obtain lethal dose (LD) <sub>20</sub> and <sub>50</sub> values in Robusta coffee BP 436. The study was conducted at the Tissue Culture Laboratory, Industrial and Beverage Crops Research Institute, from May 2017 to December 2018. Explants used were young leaves of Robusta coffee BP 436. Callus induction used ½ MS media with 2,4-D (4.52 μM) and 2-iP (0.00; 4.93; 9.86; 14.79; and 19.72 μM) treatment. Mutation induction was performed using gamma radiation dosed at 0, 10, 20, 30, 40, and 50 Gy treatments. The regeneration media was ½ MS containing GA<sub>3</sub> (0 and 1 mg/l). The study used a complete randomized design with 10 replications. The results showed the best combination of plant growth regulator to induce the callus was 2.4-D 4.52 μM + 2-iP 19.72 μM. The fresh weight of cultures was inhibited above 30 Gy, whereas the number of somatic embryos decreased at doses above 10 Gy. Addition of GA<sub>3</sub> 1 mg/l in regeneration media increased the number of somatic embryos in torpedo phase, but not in gamma irradiation exposed calluses. The LD<sub>20</sub> and LD<sub>50</sub> of Robusta coffee BP 436 are 16.81 and 28.52 Gy, respectively.</em></p>

Short Communication: Pollen diversity in the Bogor Botanic Gardens, Indonesia

Abstract. Sudarmono. 2018. Short Communication: Pollen diversity in the Bogor Botanic Gardens, Indonesia. Biodiversitas 20: 931-936. Pollen morphology influenced the process of plants evolution. Bogor Botanic Gardens has many living collections in terms of pollen diversity. The purpose of this study was observed at the diversity of pollen morphology at the Bogor Botanic Gardens and its implications for the pollen conservation of plants in the collection. The method used is cleaning the pollen using Glacial Acetic Acid (AAG), then examined using a light microscope in the tissue culture laboratory of Bogor Botanic Gardens. There were 35 specimens that were analyzed and the results were randomly shaped pollen from radial symmetry or bilateral symmetry to its combination with monocolpate, tricolpate, pericolpate and stephanocolpate. While aperture types were the monoporate type with 5 specimens, monocolpate 10 specimens, tricolpate 11 specimens, stephanocolpate 4 specimens, periporate 4 specimens, and syncolpate 1 specimen. Three specimens families Caesalpiniaceae (order Fabales) has the shape of pollen vary, i.e radial symmetry monocolpate and radial symmetry tricolpate. Similarly, two specimens of the family Araliaceae (Apiales) different shapes, namely radial symmetry and bilateral symmetry tricolpate. In Asteraceae and Asclepiadaceae has the same shape, the radial symmetry tricolpate. Overall of 35 specimens then there are 22 specimens of radial symmetry and 13 specimens of bilateral symmetry. For the same family have the same ornamentation, for example in the Lamiaceae, Malvaceae, and Rutaceae. But the uniqueness occurs in the family of Arecaceae or palm family that show the diversity in the shape and size of pollen in each genus. Pollen morphology analysis through a combination of morphological data, palynology, and molecular samples more would be better. Pollen bank as pollen in the living collections of Bogor Botanic Gardens is needed to germplasm conservation of endangered plants in Indonesia.

Automated Relative Humidity and Temperature Control System for Banana Tissue Culture Laboratory with Monitoring System and SMS Notification

The challenged to developers is to automate relative humidity and temperature which control and monitoring approaches is using SMS based on the notification as part of the technology innovation. Thus, it complicates in the banana tissue culture laboratory implementation to have efficient monitoring system supplements the concept of the study that makes it more interesting and timelier in the implementation of Internet of Things Approach. The project intent to help the owner to automatically control and monitor temperature and humidity in banana tissue culture laboratory to provide appropriate temperature and relative humidity for banana which is the basic requirements to maintain the fast growth and resistant to diseases and infection. The prototype was tested with a white-box testing approach which surpass the requirements to the real laboratory. A survey questionnaire was also being used as part of evaluating the functionality and usability of the prototype that a very agree on both are the result.

Diversification strategy in Dealing the Development of the world coffee products (Study On Coffee And Cacao Research Centers Indonesia/ICCRI )

Indonesian Coffee and Cocoa Research Institute (ICCRI) is a institution that Conducting research to obtain technological innovation in the field of cultivation and processing of coffee and cocoa products. ICCRI has an Experimental Garden and Office Area of 380 ha, consisting of arabica coffee experimental garden (KP Andungsari height of 100-1,200 m asl), robusta coffee and cocoa (KP Kaliwining and KP Sumberasin 45-550 m above sea level). The laboratory has an area of 2,365 m2 with equipment of 850 units. Consisting of Plant Breeding Laboratory, Soil Physics Laboratory, Soil Chemistry and Soil Biology, Tissue Culture Laboratory, Agricultural Mechanization Laboratory, Product Processing Laboratory, Quality Control Laboratory, Information Center and Training. The purpose of this research is to provide an overview of diversification by Indonesian Coffee and Cocoa Research Institute (ICCRI) to further develop coffee production business in Indonesia. Analysis data used in this study is discriptive analysis to describe holisticly and also SWOT analysis to discribe deeply strength weakness opportunity and threat to make strategy to improve the performance. The result of the research show that needed a diversification business conducted in the form of internal deverifikation by doing research to get a better quality coffee plant, quality and have high competitiveness at national and international level. The resulting coffee plant is also resistant to plant diseases, more fruiting, better fruit quality. Deverification is also carried out by performing aoutonomous fund with the establishment of Coffe and Cocoa science techno park which is also intended to disseminate the results and activities of ICCRI to the community.

DIRECT EMBRYOGENESIS AND INDIRECT ORGANOGENESIS OF DATE PALM (PHOENIX DACTYLIFERA L.) CV. SEWI USING IMMATURE FEMALE INFLORESCENCES

This study was achieved at the Tissue Culture Laboratory of the Agricultural Genetic Engineering Research Institute, Giza, Egypt during the period from 2013 to 2017, Direct embryo initiation and callus formation of date palm cv. Sewi from immature female inflorescences have been achieved on modified MS medium supplemented with 4 mg l-1 Picloram plus 3 mg l-1 2 iP and 2 g l-1 PVP. Results also showed that BA at 0.5 mg l-1 produce the highest number of differentiated embryos/culture, while kinetin at 0.25 mg l-1 significantly increased the average number of adventitious shoots/culture. NAA at 1.0 mg l-1 induced the highest rooting percentage and micro-shoot length. On the other hand the best survival percentage during the acclimatization stage was observed with plantlets produced from IBA at 0.5 mg l-1 during the rooting stage.

Pertumbuhan Tunas Pisang Barangan (Musa Acuminata Colla.) akibat Pemberian Benzyl Amino Purin dan Arang Aktif secara In Vitro.

Pertumbuhan Tunas Pisang Barangan Akibat Pemberian Benzyl AminoPurin dan Arang Aktif secara In Vitro Accretion of Barangan Banana Shoot Effect of (BAP) and Activated Charcoal Explant by In VitroAbstrak. Banana as a superior product of horticultural crops, has not achieved high productivity and has several obstacles in its multiplication. Tissue culture is one solution to overcome this problem. This study aims to determine the composition of PGRBenzil Amino Purine (BAP) and activated charcoal that are appropriate in the multiplication of barangan banana shoots. This research was conducted at the Tissue Culture Laboratory Faculty of Agriculture Syiah Kuala University, Darussalam Banda Aceh. The design used was a Completely Randomized Design (CRD) with two treatment factors. The first factor is BAP concentration consisting of 3 levels, namely 4 mg/L, 6 mg /L, and 8 mg/L. The second factor is activated charcoal concentration consisting of 3 levels, namely control, 1 g/L and 2 g/L. In this study, from 9 treatment combinations, only 4 treatment combinations were not contaminated. Of the 4 treatment combinations the combination of BAP concentration of 6 mg/L and control (without activated charcoal) showed the fastest shoot growth time of 29 days after multiplication, the most shoot growth was 6 shoots and the average shoot height was 15.9 mm

Growth Induction of Cavendish Buds (Musa Acuminata L.) on Difference Concentration of IBA and BA in Vitro

The propagation of Cavendish (Musa acuminata L.) seedling conventionally relatively took a longer time, not uniformed in quality and possibly has a disease that is derived from unhealthy mother stock. This research aimed to find the effect of IBA and BA on difference concentrations on the growth of plantlet, also to determine the optimum concentration of IBA and BA on the growth of Cavendish bud plantlets in vitro. This research had been carried out started from April to July 2018 in Tissue Culture Laboratory, Research Institute of Horticultural Seed Development of Pasir Banteng, Sumedang, West Java. Indonesia. The method used in this research was Completely Randomized Design with two factors. The first factor was IBA (i0 = 0 mg L-1, i1= 0,75 mg L-1, dan i2= 1,5 mg L1), and the second factor was BA (b1= 1 mg L-1 b2= 3 mg L-1 b3= 5 mg L-1 b4= 7 mg L-1), replicated three times. Duncan Multiple Range Test (DMRT) was used as a further test. The result showed an interaction between IBA and BA on the parameters of shoot growth and the number of leaves at 6 WAC. IBA showed an independent effect on the number of buds. The addition of 1,5 mg L-1 IBA and 3 mg L-1 BA was optimum for the growth of Cavendish bud explants.

PERKECAMBAHAN DAN PERTUMBUHAN PROTOKORM ANGGREK PHALAENOPSIS PADA BEBERAPA KOMBINASI KOMPOSISI MEDIA DAN AIR KELAPA

The composition of artificial media used is one of the factors that determine the speed of germination and growth of orchid seedling in the bottle. The study aims to: 1) obtain a good combination of media composition and coconut water for the germination and growth of Phalaenopsis amabilis orchids protocorm; and 2) to know whether the leaf fertilizer can replace the use of MS base media in germinating the seeds and helping in the growth of Phalaenopsis orchids protocorm. The study was conducted from September to November 2017, at the Tissue Culture Laboratory of Politeknik Negeri Lampung. There are 9 treatment combinations tested: P1 = MS without addition of coconut water, P2 = MS plus coconut water 75 ml. l-1, P3 = MS plus coconut water 150 ml. l-1, P4 = Kristalon Hijau without coconut water, P5 = Kristalon Hijau plus 75 ml coconut water. l-1, P6 = Kristalon Hijau plus 150 ml coconut water. l-1, P7 = Growmore without coconut water, P8 = Growmore plus 75 ml coconut water. l-1, and P9 = Growmore plus 150 ml of coconut water. l-1. Observations were made on the time needed for the seeds to germinate and the growth speed of protocorm until they were ready to be subcultured. The results of this research showed: 1) The best media composition for the germination and growth of Phalaenopsis orchid protocorm is basic media MS with 75 ml coconut water addition. l-1 followed by MS media with the addition of 150 ml of coconut water. l-1; 2) Kristalon Hijau Fertilizers with the addition of coconut water 75 ml. l-1 as well 150 ml. l-1 can be used for germination and growing of Phalaenopsis orchid protocorm to become seedling, but with longer time needed than MS medium.

The Application of Adaptive Concept Form of Tissue Culture Laboratory Building in Black Orchid Research and Development Center in Samarinda

Black Orchid is an endangered species in East Kalimantan in the last five years. If it’s not conserved, this endemic species will extinct. The research and development centre aims to research and develop black orchids so that the population is maintained. In this research and development, the main facilities are tissue culture laboratory and greenhouse with adaptive as the concept form. The adaptive concept was applied to create a form of building that can adapt to the environment, without having to reject the use of modern materials and technology. The shape of the structure based on the shape of regional architecture of East Kalimantan which adapts to the local cultural values to create harmony with the surrounding environment. The characteristic of the building has many openings, and in the front facade, there are typical of Dayak carvings with philosophy as protection of the building.

Direct organogenesis in vitro propagation of local balinese banana with thidiazuron

Bali’s community needs for bananas are very high, but cannot be fulfilled from the production of bananas in Bali. The main problem for banana cultivation in Bali is the difficulty of getting seeds.  Direct organogenesis is offered to overcome this problem.  The objective of the research was to study direct organogenesis on in vitro propagation of local Balinese banana with Thidiazuron. The research was carried out at Tissue Culture Laboratory, Faculty of Agriculture, Udayana University, Denpasar, Bali. The experiment was laid out in a completely randomized design with five treatments of thidiazuron concentration, i.e. 0; 0.5; 1.0; 1.5, and 2.0 ppm. Each treatment was replicated 5 times. The Murashige & Skoog medium added with coconut water and extract of bamboo shoots was used as a basic medium.   The local Balinese bananas named Saba and Susu were used as source of planting materials.  Explants were taken from shoot tips of suckers of newly growing plants. The results showed that the best treatment for both Saba and Susu, was 1 ppm of thidiazuron, as indicated by the highest number of plantlets produced, the number of shoots and plant height.

Multiplikasi Tunas Meriklon Kentang Pada Berbagai Jenis dan Konsentrasi Sitokinin

Mericlone shoots are shoots of propagation using meristem explants which aim to produce seeds in a short time, large number, and disease-free seed. The aim of this experiment was to find out the best types and concentration of cytokinin to mericlone shoot multiplication of potato Jala Ipam variety. The experiment was carried out at Seed Technology Tissue Culture Laboratory of Agriculture, Padjadjaran University during November 2016 until February 2017. The experimental design was Completely Randomized Design with 13 treatments and 4 replications. Murashige and Skoog Medium with different concentration and types of cytokinine was used in this experiment. The result showed that the type and concentrations of cytokinin give the different effect on the mericlone shoot multiplication of potato Jala Ipam variety. MS medium with the additional of cytokinin 2 -iP 1.5 mg L-1 significantly increased the mericlone shoot multiplication of potato Jala Ipam variety at the variable number of leaves, number of nodes, and the number of branches.

RESPONS PERTUMBUHAN TANAMAN HIAS ANTHURIUM ‘GELOMBANG CINTA’ (Anthurium plowmanii) PADA BEBERAPA KONSENTRASI BAP DAN FREKUENSI PEMBERIAN PUPUK DAUN

Ornamental plants anthurium wave love great demand of society because the leaves are beautiful, unique and interesting. The edges of the leaves of anthurium generally with characteristic wavy. Anthurium plant growth stimulation can be enhanced by the application of growth regulators BAP and foliar fertilizers. The purpose of this study was to obtain a concentration of growth regulators BAP and best foliar fertilizer for growing ornamental plants anthurium. Experiments was carried out in the plant tissue culture laboratory of the Faculty of Agriculture, Andalas University from November 2015 to February 2016. Materials used in the love wave anthurium seeds, plant media are a mixture of soil, sand, compost 1: 1: 1 (v / v). Two-factor factorial experiment arranged in completely randomized design with three replications. The first factor was the concentration of BAP (0, 2.5, 5.0 and 7.5 mg/L) and the second factor was the frequency of giving leaf fertilizer (0, 1, 2 times). The variables observed were plant height, leaf width, root length and fresh weight of plants. The data were analyzed by analysis of variance followed by Honestly Significant Difference test 5%. The results showed that there is an interaction between BAP and the frequency of leaf fertilizer application to plant height and root length. The best BAP 2.50 mg/L for leaf width growth and 5.0 mg/L best to increase the fresh weight of plants. The best BAP concentration of 2.50 mg/L for leaf width growth and 5.0 mg/L is best for increasing the fresh weight of plants. Application of leaf fertilizer with a frequency of 2 times shows the highest plant height Keywords: Anthurium, BAP, Concentration, Foliar fertilizer, Frequency

Callus Induction on True Shallot Seed Explant Using a Combination of BA and 2,4-D

BA and 2,4-D combination were commonly used for in vitro culture of Shallot (Allium cepa L. var agregatum 2n = 2x = 16) to induce callus, but there was no information for callus induction on shallot seed (TSS) explant. Callus could be utilized for in vitro selection and generating of genetic variation. The aims of the research was to identify the response of TSS (Trisula and Tuk Tuk) as explant and to obtain the optimum combination of BA and 2,4-D (mg.L-1): (0–0, 2–1, 2–2, 2–3, and 2–4) in callus induction. The research had been carried out in the Tissue Culture Laboratory, Faculty of Agriculture, Universitas Gadjah Mada during the year 2015-2016. Factorial treatments of variety and growth regulators were arranged in Completely Randomized Design with four replications. Data of percentage of germination, shoot height, root length, percentage of callus formation, callus weight, and chromosomes number of callus were recorded. The results showed that combination of 2 mg.L-1 BA + (1– 4) mg.L-1 2,4-D induced callus formation on TSS but inhibits shoots and roots growth. The best callus proliferation was at a concentration of 1 mg.L-1 2,4-D. Tetraploid callus chromosomes (2n = 4x = 32) was detected in Trisula grown in the 2 mg.L-1 BA + 4 mg.L-1 2,4-D, but in the Tuk Tuk callus did not detected the changing of chromosomes number.

In vitro shoot regeneration of mint (Mentha sp. L.) Using different types of explants and levels of benzylaminopurine

An experiment was conducted in the Tissue Culture Laboratory of the Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur-1706 from March 2013 to February 2014 for in vitro shoot regeneration of mint using different explants and levels of benzylaminopurine (BAP) in full strength MS media. Three different types of explants viz. nodal segment, shoot tip and leaf were evaluated using three levels of BAP (1.0, 2.0 and 3.0 mg/l) along with control for shoot regeneration. Results revealed that shoot tip and nodal segments performed better than leaf as explants in almost all the characters studied. Shoot tip and nodal segments initiated shoot within the shortest time of 9.6 and 10.6 days, respectively with 1.0 mg/l of BAP. Regarding number of shoot per explant and number of node/shoot, shoot tip and nodal segments along with 1.0 mg/l of BAP performed superior at almost all days after inoculation. In case of interaction of explants and BAP, better performance was recorded in most of the studied parameters from shoot tip and nodal segments alongwith 1.0 mg/l BAP. Therefore, for in vitro shoot regeneration of mint, shoot tip or nodal segment may be used with 1.0 mg/l of BAP.Bangladesh J. Agril. Res. 43(4): 703-716, December 2018

IN VITRO CALLUS INDUCTION OF ‘SHIRAZ’ GRAPE (Vitis vinifera L.) USING DIFFERENT MEDIUM AND GROWTH REGULATOR COMBINATION

One of grape cultivars widely used as raw material for wine production is ‘Shiraz’ cultivar. Propagation of this cultivar is necessary for the provision of grape as a wine making material in Bali. In vitro culture is an alternative propagation technique than can be employed to produce planting materials in a shorter time. This research aims to determine the most suitable medium and growth regulator combination in inducing grape cv. ‘Shiraz’ callus in vitro. The study was conducted from November 2017 until February 2018 at Plant Tissue Culture Laboratory, Biology Study Program, Udayana University. The explants used were young stem of grape cv. ‘Shiraz’ and the experiment was conducted using factorial design with two factors. The first factor was basal medium used (MS and WPM) and the second factor was IBA concentration (0; 0.5 and 1 mgL-1) and BAP (0, 1 and 2 mgL-1). The results showed that the highest percentage of callus induction (60%) was obtained on WPM medium without growth regulator combination (control). However, the fastest time of callus appear was on MS medium + 2 mgL-1 BAP without IBA, which was 17 days after planting. The texture and color of callus resulted on this research were friable with white, greenish white, greenish yellow and green in color.

Organogenesis of palm oil from apical bud explants

The purpose of this research is to find out explants apical bud from oil palm of different position which are apex, middle, and basal segment, were cultured in Y3 medium suplemented with 2,4-D in the presence of activated charcoal as many 3 g/L for induction organogenesis from callus inisiation. This research is conducted at Tissue Culture Laboratory, Mathematic and Natural Science Faculty, North Sumatera University from November 2013 to June 2014. In this research, experiment for organogenesis by growth regulator substance 2,4-D at concentration of 100 mg/L, 115 mg/L, 120 mg/L, 135 mg/L, and 140 mg/L. This research was designed by completely randomized design two factorials with 5 attempt. The production of organ was affected by 2,4-D concentration and explants position; 2,4-D 100 mg/L was the most effective (66%) in inducing organogenesis from the basal segment.

Somatic Embryogenesis of Melon (Cucumis melo L.) As Affected by Culture Media and Composition of Plant Growth Regulators

Conventional production of melon hybrid seeds requires a long time. Propagation through plant tissue culture can be an alternative method to produce hybrid melon seedlings in order to fulfill the high demand for uniform seedlings. Our current study was aimed to determine the type of propagation media and the best concentration of picloram for the induction of somatic embryogenesis in melon from young seed explants. The study was expanded to examine the effective concentrations of two of auxins, 2-4 D and NAA, combined with BAP to induce somatic embryogenesis in melon using hypocotyl explants. The experiment was conducted at Plant Tissue Culture Laboratory, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University. The experiment was arranged in a randomized complete block design with three replications. The first experiment tested three types of planting media (MS, B5, and WPM) and four levels of picloram concentration (0, 0.5, 1.0, 1.5 mg.L-1). The second experiment tested auxin (2,4-D and NAA) concentrations of 0, 1, 2, 3, 4, 5 mg.L-1 and two BAP concentrations, 0 and 1 mg.L-1. The first study showed that no somatic embryos were formed with the media types and picloram concentration tested; the seeds, however, germinated and formed callus. In the second study, there was an interaction between concentration levels of auxin (2,4-D and NAA) and BAP on induction of somatic embryos using hypocotyl explants. Somatic embryo formation can be induced with treatment of 1 mg.L-1 NAA + 0.1 mg.L-1 BAP and 2 mg.L-1 NAA + 0.1 mg.L-1 BAP. The highest yield of embryos formation was with the treatment of 1 mg.L-1 NAA + 0.1 mg.L-1 BAP at 0.53 embryos per explant. Keywords: embryogenesis, tissue culture media, melon, Picloram, 2,4 D, BAP

Viability estimation and morphological characterization of pollen Datura suaveolens Willd (Solanaceae)

The species Datura suaveolens W. belongs to the family Solanaceae. It is a natural shrub species of the Himalayas, is widely distributed in South America and Brazil. In Brazil it is cultivated for medicinal and ornamental purposes. The objective of this work was to perform the morphological characterization and verify the pollen viability of the D. suaveolens species by means of colorimetric tests in order to determine the best dye for the species. Flower buds of Datura suaveolens were collected with occurrence in the municipality of Alta Floresta, MT. The experiment was carried out at the Cytogenetic and Tissue Culture Laboratory at the State University of Mato Grosso, Alta Floresta University Campus. For the estimation of pollen viability, three different dyes were evaluated: lugol, Alexander’s reactive and acetic orcein. For the pollen morphology, forty pollen grains stained with 3% safranin were analyzed. The morphological parameters analyzed were: equatorial diameter, polar diameter, size and shape and opening of the pollen grains. The diameter measurements were measured with the help of ImageJ software. The Alexander’s reactive is the most recommended colorimetric method for the pollen viability analysis of Datura Suaveolens because it revealed the highest average of viable pollen and presents a better visual distinction between viable and non-viable pollen. The Datura Suaveolens species has large pollen grains with Oblate-Spheroidal shape, the opening of the pollen is 3-pore type. The mean polar diameter of the species is 70.99 μm and the equatorial diameter is 67.94 μm.

Photoautotrophic System: A Review and Potential Application for Plant Propagation In Vitro

AbstractThe standard method of in vitro plant micro propagation uses of tightly closed culture bottles using agar media containing macro and micro nutrients and sucrose as a source of carbon for the explants. The closed bottle culture is usually kept in a temperature and light controlled environment which is lower and of different quality from the natural sunlight, resulting in high relative humidity and no air exchange inside the bottles. Explants produced in vitro have malfunctioned stomata, undeveloped cuticles and lower leaf chlorophyll levels, and hyper hydration of the plantlets. Photoautotrophic tissue culture is micro propagation without or with a reduced sugar level in the culture media, so the growth or accumulation of carbohydrates of the explants is dependent fully upon photosynthesis and inorganic nutrient uptake. This method is usually combined with ventilation or CO2 enrichment, and recently, with incorporating porous materials such as vermiculite, gum or paper pulp to the agar media to promote better root system of the explants. This article discuss the advantages and disadvantages of the photoautotrophic micro propagation compared to the standard micro propagation methods, and provided the results of the photo autotrophic micro propagation studies conducted at Laboratory of Tissue Culture II of the Department of Agronomy and Horticulture, Bogor Agricultural University, Indonesia.

Transplanting Space Effect on In-vitro Raised Sugarcane

Tissue cuture is considered to be a best technique for rapid multiplication and production of disease free, healthy seed cane. With a view to studying the effect of transplanting spacing on growth and yield of micropropagated crop of sugarcane, an experiment was carried out at Tissue Culture Laboratory, Shobhit University, Meerut. Tissue culture raised plantlets of sugarcane variety. In vitro cultured sugarcane were transplanted at various spacing of 90 x 45, 90 x 60, 90 x 90 and 120 x 60 cm. Among the four spacings, the highest plant growth, number of tillers, number of malleable canes, cane height and cane yield were recorded at 90 x 60 cm. Thus, a spacing of 90 x 60 cm was found most suitable for transplantation of tissue culture raised plantlets of sugarcane