Abstract
One of grape cultivars widely used as raw material for wine production is ‘Shiraz’ cultivar. Propagation of this cultivar is necessary for the provision of grape as a wine making material in Bali. In vitro culture is an alternative propagation technique than can be employed to produce planting materials in a shorter time. This research aims to determine the most suitable medium and growth regulator combination in inducing grape cv. ‘Shiraz’ callus in vitro. The study was conducted from November 2017 until February 2018 at Plant Tissue Culture Laboratory, Biology Study Program, Udayana University. The explants used were young stem of grape cv. ‘Shiraz’ and the experiment was conducted using factorial design with two factors. The first factor was basal medium used (MS and WPM) and the second factor was IBA concentration (0; 0.5 and 1 mgL-1) and BAP (0, 1 and 2 mgL-1). The results showed that the highest percentage of callus induction (60%) was obtained on WPM medium without growth regulator combination (control). However, the fastest time of callus appear was on MS medium + 2 mgL-1 BAP without IBA, which was 17 days after planting. The texture and color of callus resulted on this research were friable with white, greenish white, greenish yellow and green in color.
Highlights
Grape (Vitis vinifera L.) is one of edible fruit plants belong to Vitaceae family with woody stem, cylindrical shape, rough surface and brownish color
One of grape cultivars widely used as raw material for wine production is ‘Shiraz’ cultivar
The results showed that the highest percentage of callus induction (60%) was obtained on WPM medium without growth regulator combination
Summary
Grape (Vitis vinifera L.) is one of edible fruit plants belong to Vitaceae family with woody stem, cylindrical shape, rough surface and brownish color. One of grape cultivars that is widely used as raw material for wine production but not yet widely cultivated in Bali is ‘Shiraz’ cultivar. An alternative propagation technique such as in vitro culture is needed to obtain plant materials in a relatively shorter time and produce more plantlets. Explants were surface was able to produce in vitro shoot sterilized with 50% Bayclin with addition of propagation in Vitis thunbergii plants 2 drop of Tween 20 for 3 minutes compared with using of MS and NN medium. The young stem explants were cut on MS and WPM medium, on growth into 0.5 cm pieces and cultured on MS and regulators combination. WPM medium supplemented with 30 gL-1 sucrose, 1.5 gL-1 activated charcoal and 8 gL-
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