Abstract
Evaluation of somaclones in the field of three potato varieties (Diamant, Multa and Cardinal) were examined through callus culture using node, internode and leaf segments. Multiple shoot regeneration from callus at various frequencies was observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction from internode was observed in MS medium containing 3.0 mg l-1 of 2,4-D + 150 mg l-1 of L-asparagine. In this combination, the percentage of callusing was 90% for Diamant, 80% for Multa and 70% for Cardinal. The best response for multiple shoot formation was observed in MS medium supplemented with 4.0 mg l-1 of KIN + 0.5 mg l-1 of NAA. Diamant responded better than Multa and Cardinal for shoot regeneration from callus. Best rooting was observed from node or internode derived plantlets of primary calliclone in MS medium supplemented with IBA. Rooted calliclones were transplanted in the field and evaluated somaclones variation using parameters viz. plant height, number of leaves/plant, number of tuber/plant and tuber weight/plant. The result of statistical analysis on individual character supports existence of significant variation among the different somaclones.
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