Shear Horizontal Surface Acoustic Wave Research Articles

Giant nonreciprocity of surface acoustic waves induced by an anti-magnetostrictive bilayer

Lack of nonreciprocity is one of the major drawbacks of solid-state acoustic devices, which has hindered the development of microwave-frequency acoustic isolators and circulators. Here, we report a giant nonreciprocal transmission of shear-horizontal surface acoustic waves (SH-SAWs) on a LiTaO3 substrate coated with a negative–positive magnetostrictive bilayer structure of Ni/Ti/FeCoSiB. Although the static magnetic moments of two layers are parallel, SH-SAWs can excite optical-mode spin waves much stronger than acoustic-mode ones at relatively low frequencies via magnetoelastic coupling. The measured magnitude nonreciprocity exceeds 40 dB (or 80 dB/mm) at 2.333 GHz. In addition, maximum nonreciprocal phase accumulation reaches 188° (376°/mm), which is desired for an effective SAW circulator. Our theoretical model and calculations provide an insight into the observed phenomena and demonstrate a pathway for further improvement of nonreciprocal acoustic devices.

Ultrasound: A new strategy for artificial synapses modulation

AbstractDue to its non‐invasive nature, ultrasound has been widely used for neuromodulation in biological systems, where its application influences the synaptic weights and the process of neurotransmitter delivery. However, such modulation has not been emulated in physical devices. Memristors are ideal electrical components for artificial synapses, but up till now they are hardly reported to respond to ultrasound signals. Here we design and fabricate a HfOx‐based memristor on 64°Y‐X LiNbO3 single crystal substrate, and successfully realize artificial synapses modulation by shear‐horizontal surface acoustic wave (SH‐SAW). It is a prominent short‐term resistance modulation, where ultrasound has been shown to cause resistance drop for various resistance states, which could fully recover after the ultrasound is shut off. The physical mechanism illustrates that ultrasound induced polarization potential in the HfOx dielectric layer acts on the Schottky barrier, leading to the resistance drop. The emulation of neuron firing frequency modulation through ultrasound signals is demonstrated. Moreover, the joint application of ultrasound and electric voltage yields fruitful functionalities, such as the enhancement of resistance window and synaptic plasticity through ultrasound application. All these promising results provide a new strategy for artificial synapses modulation, and also further advance neuromorphic devices toward system applications.image

Large nonreciprocity of shear-horizontal surface acoustic waves induced by a magnetoelastic bilayer

Large nonreciprocity of shear-horizontal surface acoustic waves induced by a magnetoelastic bilayer

New Advances in Rapid Pretreatment for Small Dense LDL Cholesterol Measurement Using Shear Horizontal Surface Acoustic Wave (SH-SAW) Technology.

Atherosclerosis is an inflammatory disease of the arteries associated with alterations in lipid and other metabolism and is a major cause of cardiovascular disease (CVD). LDL consists of several subclasses with different sizes, densities, and physicochemical compositions. Small dense LDL (sd-LDL) is a subclass of LDL. There is growing evidence that sd-LDL-C is associated with CVD risk, metabolic dysregulation, and several pathophysiological processes. In this study, we present a straightforward membrane device filtration method that can be performed with simple laboratory methods to directly determine sd-LDL in serum without the need for specialized equipment. The method consists of three steps: first, the precipitation of lipoproteins with magnesium harpin; second, the collection of effluent from a 100 nm filter; and third, the quantification of sd-LDL-ApoB in the effluent with an SH-SAW biosensor. There was a good correlation between ApoB values obtained using the centrifugation (y = 1.0411x + 12.96, r = 0.82, n = 20) and filtration (y = 1.0633x + 15.13, r = 0.88, n = 20) methods and commercially available sd-LDL-C assay values. In addition to the filtrate method, there was also a close correlation between sd-LDL-C and ELISA assay values (y = 1.0483x - 4489, r = 0.88, n = 20). The filtration treatment method also showed a high correlation with LDL subfractions and NMR spectra ApoB measurements (y = 2.4846x + 4.637, r = 0.89, n = 20). The presence of sd-LDL-ApoB in the effluent was also confirmed by ELISA assay. These results suggest that this filtration method is a simple and promising pretreatment for use with the SH-SAW biosensor as a rapid in vitro diagnostic (IVD) method for predicting sd-LDL concentrations. Overall, we propose a very sensitive and specific SH-SAW biosensor with the ApoB antibody in its sensitive region to monitor sd-LDL levels by employing a simple delay-time phase shifted SH-SAW device. In conclusion, based on the demonstration of our study, the SH-SAW biosensor could be a strong candidate for the future measurement of sd-LDL.

Integrated Rayleigh wave streaming-enhanced sensitivity of shear horizontal surface acoustic wave biosensors

Shear horizontal surface acoustic wave (SH-SAW) sensors are regarded as a promising alternative for label-free, sensitive, real time and low-cost detection. Nevertheless, achieving high sensitivity with SH-SAW has approached its limit imposed by the mass transport and probe-target affinity. We present here an SH-SAW biosensor accompanied by a unique Rayleigh wave-based actuator. The platform assembled on an ST-quartz substrate consists of dual-channel SH-SAW delay lines fabricated along a 90°-rotated direction, whilst another interdigital electrode (IDT) is orthogonally placed to generate Rayleigh waves so as to induce favourable streaming in the bio-chamber, enhancing the binding efficiency of the bio-target. Theoretical foundation and simulation have shown that Rayleigh acoustic streaming generates a level of agitation that accelerates the mass transport of the biomolecules to the surface. A fourfold improvement in sensitivity is achieved compared with conventional SH-SAW biosensors by means of complementary DNA hybridization with the aid of the Rayleigh wave device, giving a sensitivity level up to 6.15 Hz/(ng/mL) and a limit of detection of 0.617 ng/mL. This suggests that the proposed scheme could improve the sensitivity of SAW biosensors in real-time detection.

Sensitivity measurements for a 250 MHz quartz shear-horizontal surface acoustic wave biosensor under liquid viscous loading

Surface acoustic wave (SAW) devices have been used in biochemical assays due to their high sensitivity. The device sensitivity is a function of changes in the density and viscosity of the liquid. Here, we studied the effect of fluid viscosity using a 250 MHz quartz shear-horizontal (SH)-SAW biosensor by monitoring different concentrations of binary aqueous/glycerol solutions. In this study, the sensitivity of the biosensor was determined by fitting the data to models derived from perturbation theory. Measurements in water were used as the reference. For a 0% to 50% glycerol solution, an 87°–204° separation in the phase shift was observed. The slope of the plot of the phase shift vs (ηρ)0.5 was used to indicate the sensor’s sensitivity. The sensitivity for our 250 MHz quartz SH-SAW sensors was calculated to be 3.7×10−3m2sKg. The corresponding mass sensitivity was determined to be 9.25 × 105m2Kg. The limit of detection was calculated to be 36 picograms (pg), while the limit of quantification or LOQ was calculated to be 109 pg. Traditionally, liquid phase measurements have been challenging for SAW devices because liquids dampen the vibrating sensors severely. This problem has been largely solved using a transverse (shear) wave instead of the more popular longitudinal or Rayleigh waves. Liquid measurements are now possible using transverse waves, also known as shear waves, because transverse waves are only minimally attenuated by liquids. Shear-horizontal SAW sensors (SH-SAW) show great promise as label-free biosensors because of their ability to handle liquid samples. However, the viscosity of the liquid still induces loading effects and can be measured when the liquid is loaded onto the SH-SAW propagating surface (delay line). When the liquid above the delay line is perturbed by physical or chemical changes, such as binding to a receptor, it alters the propagating acoustic wave. The SH-SAW device can measure these changes in liquid properties as a change in the wave’s phase compared to the original wave. The device’s phase shift was recorded as a function of the changes in the density and viscosity of the binary glycerol solution and used to determine the sensitivity in the linear dynamic range of responses.

5.7 GHz Ultrasensitive Shear Horizontal-Surface Acoustic Wave Humidity Sensor Based on LiNbO3/SiO2/SiC Heterostructures with a Sensitive Layer of Polyethyleneimine-SiO2 Nanocomposites.

Humidity sensing and water molecule monitoring have become hot research topics attributed to their potential applications in monitoring breathing/physiological conditions of humans, air conditioning in greenhouses, and soil moisture in agriculture. However, there is a huge challenge for highly sensitive and precision humidity detection with wireless and fast responsive capabilities. In this work, a hybrid/synergistic strategy was proposed using a LiNbO3/SiO2/SiC heterostructure to generate shear-horizontal (SH) surface acoustic waves (SAWs) and using a nanocomposite of polyethylenimine-silicon dioxide nanoparticles (PEI-SiO2 NPs) to form a sensitive layer, thus achieving an ultrahigh sensitivity of SAW humidity sensors. Ultrahigh frequencies (1∼15 GHz) of SAW devices were obtained on a high-velocity heterostructure of LiNbO3/SiO2/SiC. Among the multimodal wave modes, we selected SH waves for humidity sensing and achieved a high mass-sensitivity of 5383 MHz · mm2 · μg-1. With the PEI-SiO2 NP composite as the sensitive layer, an ultrahigh sensitivity of 2.02 MHz/% RH was obtained, which is two orders of magnitude higher than those of the conventional SAW humidity sensors (∼202.5 MHz frequency) within a humidity range of 20-80% RH.

Ultrahigh frequency shear-horizontal acoustic wave humidity sensor with ternary nanocomposite sensing layer

Surface acoustic wave (SAW) technology is promising for humidity monitoring due to its digital output, small size, large-scale production and wireless passive capability, but there are major challenges to achieve ultra-high sensitivity and fast responses using the conventional SAW devices. Herein, ultrahigh frequency (4.7 GHz and 5.9 GHz) shear-horizontal (SH) SAW devices were developed and a ternary nanocomposite strategy of graphene quantum dots/polyethyleneimine/silicon dioxide nanoparticles (GQDs-PEI-SiO2 NPs) was proposed as a sensitive layer to achieve ultrahigh sensitivity and fast response. This ternary material system was constructed by modifying the surface of SiO2 NPs with the PEI through an electrostatic force, and then adsorbing the GQDs onto the PEI through hydrogen bonds. Compared with the conventional low frequency SAW devices, the ultrahigh frequency SH-SAW devices showed exceptionally ultra-high sensitivity (2.4 MHz/%RH, 1000 times as high as a 202 MHz SAW device), fast response (20 s/5 s), excellent linearity, and good repeatability in the range of 20–80% RH. These superior performances are attributed to ultrahigh frequency of SAW devices, large specific surface areas of the nanocomposite (which exposed multiple hydrophilic groups in PEI and GQDs), and high vapor pressure of convex spherical curved liquid surface (which accelerated the adsorption and desorption of water molecules).

Application of Shear Horizontal Surface Acoustic Wave (SH-SAW) Immunosensor in Point-of-Care Diagnosis.

Point-of-care testing (POCT), also known as on-site or near-patient testing, has been exploding in the last 20 years. A favorable POCT device requires minimal sample handling (e.g., finger-prick samples, but plasma for analysis), minimal sample volume (e.g., one drop of blood), and very fast results. Shear horizontal surface acoustic wave (SH-SAW) biosensors have attracted a lot of attention as one of the effective solutions to complete whole blood measurements in less than 3 min, while providing a low-cost and small-sized device. This review provides an overview of the SH-SAW biosensor system that has been successfully commercialized for medical use. Three unique features of the system are a disposable test cartridge with an SH-SAW sensor chip, a mass-produced bio-coating, and a palm-sized reader. This paper first discusses the characteristics and performance of the SH-SAW sensor system. Subsequently, the method of cross-linking biomaterials and the analysis of SH-SAW real-time signals are investigated, and the detection range and detection limit are presented.

Giant spin-vorticity coupling excited by shear-horizontal surface acoustic waves

A non-magnetic layer can inject spin-polarized currents into an adjacent ferromagnetic layer via spin vorticity coupling (SVC), inducing spin wave resonance (SWR). In this work, we present the theoretical model of SWR generated by shear-horizontal surface acoustic wave (SH-SAW) via SVC, which contains distinct vorticities from well-studied Rayleigh SAW. Both Rayleigh- and SH-SAW delay lines have been designed and fabricated with a Ni81Fe19/Cu bilayer integrated on ST-cut quartz. Given the same wavelength, the measured power absorption of SH-SAW is four orders of magnitudes higher than that of the Rayleigh SAW. In addition, a high-order frequency dependence of the SWR is observed in the SH-SAW, indicating SVC can be strong enough to compare with magnetoelastic coupling.

Resonance properties of shear-horizontal surface acoustic waves on Ca3TaGa3Si2O14 at room and high temperatures

The resonance properties of shear-horizontal surface acoustic waves on Ca3TaGa3Si2O14 (CTGS) with Au or Al interdigital transducers (IDTs) were investigated. IDT-type resonators were fabricated on CTGS (0°, 134° or 155°, 90°) using Au- or Al-IDT, and the strong resonance properties and near-zero temperature coefficient of frequency (TCF) were measured at a certain electrode film thickness. From the measured and simulated results, an effective coupling factor of approximately 1.2% and zero TCF can be simultaneously obtained by using not only a high-density Au-IDT but also an Al-IDT and by adjusting the cut angle and electrode film thickness. Moreover, the resonance properties on Au-IDT/CTGS were evaluated at temperatures up to 570 °C. From the cut-angle dependences of the frequency shift calculated using the determined second-order temperature coefficients of the elastic constants, the potential for sensor applications that measure not only temperature but also pressure in such high-temperature environments can be expected.

Gigahertz Acoustic Delay Lines in Lithium Niobate on Silicon Carbide With Propagation-Q of 11174

This work demonstrates gigahertz wideband acoustic delay lines (ADLs) with record-breaking propagation-Q using a thin-film X-cut lithium niobate on silicon carbide (LiNbO3-on-SiC) platform. Benefiting from the high bulk wave velocity and excellent mechanical <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\text{f}\times \text{Q}$ </tex-math></inline-formula> of SiC, the shear horizontal surface acoustic wave (SH-SAW) excited by unidirectional transducers propagates in the top-surface of LiNbO3-on-SiC with low acoustic loss. The zero power flow angle (PFA) of −3° to +Y axis is obtained through simulation analysis and experiment validation, which leads to acoustic wave transmission perpendicular to the electrodes. Oriented at zero PFA, the fabricated ADLs show scalable center frequencies from 1.19 GHz to 2.11 GHz, 3-dB fractional bandwidth ranging from 2.7% to 11.5%, and a record-high propagation-Q of 11174. The performance has shown the great potential of the LiNbO3-on-SiC acoustic platform for various signal processing applications.

Exploring Low-Loss Surface Acoustic Wave Devices on Heterogeneous Substrates.

This article presents shear horizontal surface acoustic wave (SH-SAW) devices with excellent temperature stability and low loss on ultrathin Y42-cut lithium tantalate film on sapphire substrate (LiTaO3-on-sapphire, LTOS). The demonstrated resonators exhibit scalable resonances from 1.76 to 3.17 GHz, effective electromechanical coupling coefficients between 5.1% and 7.6%, and quality factors (Bode-Q) between 419 and 3019. The filter with a center frequency of 3.26 GHz features a suppressed spurious passband, a 3-dB fractional bandwidth (FBW) of 3%, and a minimum insertion loss (IL) of 2.39 dB. In addition, coplanar waveguides (CPWs) and SH-SAW resonators built on LTOS and LiTaO3-on-insulator (LTOI) substrates were compared over a temperature range of 25 °C-150 °C. Due to the extremely high resistivity of the sapphire and the excellent thermal stability of the LiTaO3/sapphire interface, the IL of the CPW and the impedance ratio (in addition to Bode-Q) of the SH-SAW on the LTOS are maintained well even at 150 °C, while those on the LTOI seriously deteriorate. Of these, the impedance attenuation of LTOS-SAW at the antiresonant frequency is only 3.7 dB at 150 °C, whereas that of LTOI-SAW reaches 9.6 dB, demonstrating excellent temperature stability of the LTOS substrate's radio frequency (RF) performance. Overall, the SAW devices on LTOS substrates show great potential for temperature-sensitive and low-loss applications in RF wireless communications.

Identification and Quantitation of Aqueous Single- and Multianalyte Solutions of the Isomers Ethylbenzene, m-, p-, and o-Xylene Using a Single Specifically Tailored Sensor Coating and Estimation Theory-Based Signal Processing.

The isomer-specific detection and quantitation of m-, p-, and o-xylene and ethylbenzene, dissolved singly and as mixtures in aqueous solutions at concentrations from 100 to 1200 ppb by volume, is reported for a specifically designed polymer-plasticizer coating on a shear-horizontal surface acoustic wave (SH-SAW) device. The polystyrene-ditridecyl phthalate-blend coating was designed utilizing Hansen solubility parameters and considering the dipole moment and polarizability of the analytical targets and coating components to optimize the affinity of the sensor coating for the four chemical isomers. The two key coating sorption properties, sensitivity and response time constant, are determined by the (slightly different) dipole moments and polarizabilities of the four target analytes: as analyte dipole moment decreases, coating sensitivity increases; as analyte polarizability decreases, coating response time lengthens. Using the measured sensitivities and time constants for the targets, sensor signals were processed with exponentially weighted recursive-least-squares estimation (EW-RLSE) to identify (with near 100% accuracy) and quantify (with ± 5-7% accuracy) the isomers. This impressive performance was achieved by combining the specifically tailored, high-sensitivity coating and an SH-SAW platform (yielding a detection limit of 5 ppb for the analytes) and using the EW-RLS estimator, which estimates unknown parameters accurately even in the presence of measurement noise and for analytes with only minor differences in response. Identification of the xylene isomers is important for applications including environmental monitoring and chemical manufacturing.

High-performance SH-SAW resonator using optimized 30° YX-LiNbO3/SiO2/Si

With the rapid development of 5G technology, acoustic wave filters with large bandwidths are urgently required to deal with the explosive increase in data traffic. Recently, there is extensive attention paid to shear-horizontal (SH) surface acoustic wave (SAW) resonators based on lithium niobate (LiNbO3) substrates, thanks to its large effective coupling coefficient (k2eff). However, because of the bulk acoustic wave (BAW) energy radiation into the LiNbO3 substrate, it is very challenging to obtain a high quality factor (Q) for SH-SAW resonators. In this study, a 30° YX-LiNbO3/SiO2/Si SAW resonator with the SH mode is proposed to achieve a large coupling and a high Q simultaneously. By bonding a LiNbO3 thin film onto a thermally oxidized Si(100) substrate, the velocity mismatch between the piezoelectric layer and the SiO2/Si substrate could significantly reduce the BAW energy leakage. Finite element method simulation is employed to optimize the cut angle of the LiNbO3 film and the thickness of each layer. The fabricated SH-SAW resonators with a resonant frequency of 924 MHz yield a k2eff of 24.8% and a maximum of Bode-Q (Bode-Qmax) of 1107. In comparison with the previously reported same-type SAW resonators, a higher Bode-Qmax is demonstrated in this work when their k2eff is larger than 20%, providing a potential solution to enable wideband tunable filters in the 5G communication system.

Measuring lubricant viscosity at a surface and in a bearing film using shear-horizontal surface acoustic waves

The lubricating effectiveness of an oil film in a journal bearing depends on the dynamic viscosity of the oil. The viscosity in turn depends on the local operating temperature, pressure, and shear rate. Reproducing these conditions in a laboratory viscometer to investigate the lubricant behaviour is a challenging task. As a result, methods that allow oil viscosity measurement in-situ in a film, would be preferred. Ultrasound technology utilising shear bulk acoustic waves (BAW) has been used to measure liquid viscosity in the bulk, as well as in-situ in a film; the reflection of a shear BAW from a solid-liquid interface depends on the liquid viscosity. Surface acoustic waves (SAW) have been also used for measuring bulk liquid viscosity. In this paper, shear-horizontal surface acoustic waves (SH-SAWs) were explored for measuring oil film viscosity, as they present good coupling with liquids and sensitivity to surface changes. The main objectives of this work were to generate SH-SAWs on metallic media, investigate the wave response at the metal-oil interface, relate the wave response to viscosity with the aim to apply this knowledge to a journal bearing application for measuring viscosity in-situ the lubricant film. Initially, the SH-SAW response was investigated at a solid-liquid interface. SH-SAWs attenuate at the solid-liquid interface, due to the liquid viscosity. This was modelled as a function of the liquid properties, material and geometry of the medium, and wave frequency. The SH-SAW attenuation-viscosity model was used to calculate the viscosity (in the range of ∼3 to 4600 cP) of different oils at a free surface, which agreed with the viscosity values from datasheets and bench-top viscosity measurements. This approach was then implemented in-situ in a journal bearing application. A bearing sleeve was instrumented with a pair of SH-SAW transducers and a shear BAW transducer installed inside the rotating journal. These two approaches were used to measure the film viscosity of 4 lubricants blended with different additives in two ways; the former by the leakage of the surface wave, and the latter by the reflection of the bulk wave. Both approaches were found to be in good agreement. They successfully distinguished the chemistry of the oil test samples according to their viscosities under various loading conditions and constant speed, and were able to monitor changes in the oil film viscosity in the loaded region. The SH-SAW sensors used were low cost and small sized and so can be fitted relatively conveniently into a bearing sleeve, requiring nothing but a function generator and digitiser to operate. This approach could then be used to evaluate lubricant formulations for their performance actually inside a bearing, rather than through the extrapolation of data from a conventional bench top viscometer.

Rayleigh and shear-horizontal surface acoustic waves simultaneously generated in inclined ZnO films for acoustofluidic lab-on-a-chip

There are significant challenges in controlling uniformity of crystal inclination angles, growth orientations and film thicknesses to generate dual-mode surface acoustic waves (e.g., Rayleigh ones and shear-horizontal ones) for lab-on-a-chip applications. In this study, we demonstrate large area (up to three inches) and uniformly inclined piezoelectric ZnO films, sputtering-deposited on silicon using a glancing angle deposition method. Characterization using X-ray diffraction showed that the inclined ZnO films have an average crystal inclination angle of 29.0°, apart from the vertical (0002) orientation, at a substrate tilting angle of 30o. Reflection signals of ZnO/Si surface acoustic wave devices clearly show the generations of both shear horizontal surface acoustic waves and Rayleigh waves. The Rayleigh waves enable efficient acoustofluidic functions including streaming and transportation of sessile droplets. Excitation direction of Rayleigh waves on the acoustofluidics versus the inclined angle direction has apparent influences on the acoustofluidic performance due to the anisotropic microstructures of the inclined films. The same device has been used to demonstrate biosensing of biotin/streptavidin interactions in a liquid environment using the shear-horizontal surface acoustic waves, to demonstrate its potential for integration into a complete lab-on-a-chip device.

Carbon SH-SAW-Based Electronic Nose to Discriminate and Classify Sub-ppm NO2.

In this research, a compact electronic nose (e-nose) based on a shear horizontal surface acoustic wave (SH-SAW) sensor array is proposed for the NO2 detection, classification and discrimination among some of the most relevant surrounding toxic chemicals, such as carbon monoxide (CO), ammonia (NH3), benzene (C6H6) and acetone (C3H6O). Carbon-based nanostructured materials (CBNm), such as mesoporous carbon (MC), reduced graphene oxide (rGO), graphene oxide (GO) and polydopamine/reduced graphene oxide (PDA/rGO) are deposited as a sensitive layer with controlled spray and Langmuir–Blodgett techniques. We show the potential of the mass loading and elastic effects of the CBNm to enhance the detection, the classification and the discrimination of NO2 among different gases by using Machine Learning (ML) techniques (e.g., PCA, LDA and KNN). The small dimensions and low cost make this analytical system a promising candidate for the on-site discrimination of sub-ppm NO2.

Novel SH-SAW Biosensors for Ultra-Fast Recognition of Growth Factors.

In this study, we investigated a label-free time efficient biosensor to recognize growth factors (GF) in real time, which are of gran interesting in the regulation of cell division and tissue proliferation. The sensor is based on a system of shear horizontal surface acoustic wave (SH-SAW) immunosensor combined with a microfluidic chip, which detects GF samples in a dynamic mode. In order to prove this method, to our knowledge not previously used for this type of compounds, two different GFs were tested by two immunoreactions: neurotrophin-3 and fibroblast growth factor-2 using its polyclonal antibodies. GF detection was conducted via an enhanced sequential workflow to improve total test time of the immunoassay, which shows that this type of biosensor is a very promising method for ultra-fast recognition of these biomolecules due to its great advantages: portability, simplicity of use, reusability, low cost, and detection within a relatively short period of time. Finally, the biosensor is able to detect FGF-2 growth factor in a concentration wide range, from 1–25 µg/mL, for a total test time of ~15 min with a LOD of 130 ng/mL.

A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW).

Since the Coronavirus disease 2019 (COVID-19) pandemic outbreak, many methods have been used to detect antigens or antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including viral culture, nucleic acid test, and immunoassay. The shear-horizontal surface acoustic wave (SH-SAW) biosensor is a novel pathogen detection platform with the advantages of high sensitivity and short detection time. The objective of this study is to develop a SH-SAW biosensor to detect the anti-SARS-CoV-2 nucleocapsid antibody. The rabbit sera collected from rabbits on different days after SARS-CoV-2 N protein injection were evaluated by SH-SAW biosensor and enzyme-linked immunosorbent assay (ELISA). The results showed that the SH-SAW biosensor achieved a high correlation coefficient (R = 0.9997) with different concentrations (34.375–1100 ng/mL) of the “spike-in” anti-N protein antibodies. Compared to ELISA, the SH-SAW biosensor has better sensitivity and can detect anti-N protein IgG signals earlier than ELISA on day 6 (p < 0.05). Overall, in this study, we demonstrated that the SH-SAW biosensor is a promising platform for rapid in vitro diagnostic (IVD) testing, especially for antigen or antibody testing.