Novel SH-SAW Biosensors for Ultra-Fast Recognition of Growth Factors.

Daniel Matatagui,Ágatha Bastida,M Carmen Horrillo

doi:10.3390/bios12010017

Abstract

In this study, we investigated a label-free time efficient biosensor to recognize growth factors (GF) in real time, which are of gran interesting in the regulation of cell division and tissue proliferation. The sensor is based on a system of shear horizontal surface acoustic wave (SH-SAW) immunosensor combined with a microfluidic chip, which detects GF samples in a dynamic mode. In order to prove this method, to our knowledge not previously used for this type of compounds, two different GFs were tested by two immunoreactions: neurotrophin-3 and fibroblast growth factor-2 using its polyclonal antibodies. GF detection was conducted via an enhanced sequential workflow to improve total test time of the immunoassay, which shows that this type of biosensor is a very promising method for ultra-fast recognition of these biomolecules due to its great advantages: portability, simplicity of use, reusability, low cost, and detection within a relatively short period of time. Finally, the biosensor is able to detect FGF-2 growth factor in a concentration wide range, from 1–25 µg/mL, for a total test time of ~15 min with a LOD of 130 ng/mL.

Highlights

Growth factors (GFs) are biomolecules which are involved in a variety of cellular processes, such as proliferation, anti-apoptosis, drug resistance, and angiogenesis [1]
In the case of fibroblast growth factor-2 (FGF-2) detection, anti-FGF-2 protein was incorporated in the cone the system, with a frequency decrease around 12.6 kHz observed due to the binding amino groups of anti-FGF-2 with the aldehyde groups anchored on the device surfac which was very similar to the case of the anti-NT-3 (both antibodies have similar weigh Afterward, BSA protein was added to block aldehyde groups not bound to the a ti-FGF-2 (Figure 5)
This article describes the detection of GFs, in a very fast and sensitive way and without the need for labeling, using shear horizontal surface acoustic wave (SH-SAW) sensors

Summary

Introduction

Growth factors (GFs) are biomolecules which are involved in a variety of cellular processes, such as proliferation, anti-apoptosis, drug resistance, and angiogenesis [1]. The growth factor FGF-2 is a member of a large family of proteins that binds heparin and heparan sulfate which modulate the function of a wide range of cell types. These techniques have large processing, use many reactives, and work with high volumes, being, slow and expensive techniques To solve these important inconvenients, in this work, shear horizontal surface acoustic wave (SH-SAW) immunosensors were used, since they show relevant advantages, such as high sensitivity, low cost, low power, ultra-fast recognition, and real-time monitoring, features of great interest for different fields of application [20], such as environment [21], security, food, and biomedicine [22]. We have detected NT-3 and FGF-2 proteins as suitable biological markers for metastasis using SH-SAW ultra-fast devices with microfluidics systems

Materials

System Temperature Control

Conclusions