Protein-like Substances Research Articles

C4-HSL-mediated quorum sensing regulates nitrogen removal in activated sludge process at Low temperatures

The activated sludge process faces challenges in achieving adequate nitrification ability under low-temperature conditions. Therefore, we investigated the effects of different concentrations of exogenous N-butyryl-homoserine lactone (C4-HSL) on nitrogen removal in lab-scale sequencing batch reactors (SBRs) at 10 °C. The results revealed that both 10 and 100 μg/L of C4-HSL could improve NH4+-N removal efficiency by 26% and reduce the effluent TN concentration to below 15 mg/L. Analysis of extracellular polymeric substances (EPS) revealed that adding C4-HSL (especially 100 μg/L) reduced the protein-like substance content while increasing the humic and fulvic acid-like substance content in EPS. Protein-like substances could serve as carbon sources for denitrifiers, thus promoting denitrification. Moreover, exogenous C4-HSL increased the abundance of bacteria and genes associated with nitrification and denitrification. Further analysis of quorum sensing (QS) of microorganisms indicated that exogenous C4-HSL (especially 100 μg/L) promoted regulation, transportation, and decomposition functions in the QS process. Furthermore, CS, sdh, fum, and mdh gene expressions involved in the tricarboxylic acid (TCA) cycle were enhanced by 100 μg/L C4-HSL. Exogenous C4-HSL promoted microbial communication, microbial energy metabolism, and nitrogen metabolism, thereby improving the nitrogen removal efficiency of activated sludge systems at low temperatures. This study provides a feasible strategy for enhancing denitrogenation performance at low temperatures through exogenous C4-HSL.

Effects of adding steel slag on humification and characteristics of bacterial community during phosphate-amended composting of municipal sludge

This study aimed to investigate the effects of different proportions (0%, 5%, 7.5%, and 10%) of steel slag (SS) on humification and bacterial community characteristics during phosphate-amended composting of municipal sludge. Compared with adding KH2PO4 alone, co-adding SS significantly promoted the temperature, pH, nitrification, and critical enzyme activities (polyphenol oxidase, cellulase, laccase); especially organic matter (OM) degradation rate (25.5%) and humification degree (1.8) were highest in the 5%-SS treatment. Excitation-emission matrix-parallel factor confirmed that co-adding SS could promote the conversion of protein-like substances or microbial by-products into humic-like substances. Furthermore, adding 5%-SS significantly improved the relative abundances of Actinobacteria, Firmicutes and the genes related to carbohydrate and amino acid metabolism, and enhanced the interactions of bacterial community in stability and complexity. The partial least squares path model indicated that OM was the primary factor affecting humification. These results provided a promising strategy to optimize composting of municipal sludge via SS.

Composition characteristics and metal binding behavior of macrophyte-derived DOM (MDOM) under microbial combined photodegradation: A state closer to actual macrophytic lakes

In actual lakes, the “unstable components” of macrophyte-derived DOM (MDOM) are always degraded and cannot exist abidingly, but the environmental impact brought by it is ignored. In this study, MDOM from Potamogeton crispus was extracted to carry out microbial combined photodegradation (M-Photodegradation) and fluorescence titration experiments. Then the traits and metal binding reaction of MDOM under M-Photodegradation were analysed and compared with the features of lake-derived DOM (LDOM) from point monitoring of Dongping Lake through EEM-PARAFAC, 2D-SF-COS, and 2D-FTIR-COS. The results showed that the features of MDOM after M-Photodegradation were closer to those of LDOM. The degradation amplitudes were 93.53% ± 0.53% for C4 in microbial degradation and 78.31% ± 0.74% for C3 in photodegradation. Correspondingly, both were hardly detected in LDOM. Protein-like substances and aliphatic C–OH were preferentially selected by Cu2+, while humic-like matter and phenolic hydroxyl O–H responded faster to Pb2+. Although the binding sequences remained unchanged after M-Photodegradation, the LogKCu and LogKPb of components decreased overall, indicating increased environmental risks. This study proves that the refractory MDOM retained after degradation was more consistent with the actual state of macrophytic lakes and provides more information for the treatment of heavy metal pollution in lakes.

Effects of additive on formation and electron transfer capacity of humic substances derived from silkworm-excrement compost during composting

Amending biochar or MnO2 is a common strategy to regulate humification during manure composting. However, how these additives affect the formation, spectrum characteristics (UV–vis, FTIR, EEM) of humic substances (HSs) in silkworm-excrement (SE) compost and their electron transfer capacities (ETC) remains unclear. Thus, the SE composting pilot separately added with 10% corncob biochar (CB) (w/w) and 0.5% MnO2 (w/w) was run to investigate the effects. The results revealed that adding 10% CB slightly affected the HA/FA (humic acids/fulvic acids) ratios, UV–vis and FTIR spectra of the final SE-compost HSs and EEM components in the FA, but remarkably improved fulvic-like (C1)/quinone-like (C3) substances and reduced humic-like (C2)/protein-like substances (C4) in the HA. Meanwhile, 0.5% MnO2 had a noticeable positive effect on the aromatization of SE-compost FA and HA but only weak impact on SUVAs and EEM components in these HSs except C4 in the FA. Moreover, 10% CB obviously reduced EAC/EDC of FA and HA in the final SE compost by 31.1%/22.0% and 19.7%/24.0%, while MnO2 improved EDC of these HSs by 6.5%/9.1% (FA/HA). These results showed MnO2 can be used as a useful amendment to enhance the promotion effect of SE-compost HA in the soil remediation other than CB. Further investigation is suggested to focus on the effects of adding MnO2 on SE-compost HSs enhancing soil remediation and its effect on ETC derived from other manure compost.

Analysis of the Effects of Surfactants on Extracellular Polymeric Substances

Reservoirs after chemical flooding usually have residual chemicals, which can affect the driving effect of subsequent microbial drives. Among them, the effect of surfactants on the metabolites of oil-recovering bacteria is the most obvious. Therefore, this paper investigates the influence mechanism of sodium dodecyl sulfate (SDS) on the nature and structure of Extracellular Polymeric Substances (EPS) produced by metabolism of Enterobacter cloacae, through a variety of characterization to analysis the components and structure of EPS under SDS stress. The results showed that Enterobacter cloacae was identified as a glycolipid-producing strain, the main components of EPS were polysaccharides and proteins. The polysaccharide composition (%: w/w) was glucosamine, 37.2; glucose, 31.5; rhamnose, 26.3; xylose, 1.7; and unidentified sugar, 3.3; and the main component of proteins was polyglutamic acid. EPS under the stress of SDS showed an increase in the content of functional groups such as -C=O and -COOH and an increase in the cellular particle size, and production of EPS increased by 10.69 × 103 mg/L when the SDS concentration was 2.5 × 102 mg/L; 3D-EEM results showed that the components of all three types of EPS The 3D-EEM results showed that all three types of EPS fractions contained tryptophan and protein-like substances, humic acid-like substances were only distributed in the solubilized extracellular polymers (SL-EPS), and aromatic proteins were only present in the loosely bound type (LB-EPS) and tightly bound type (TB-EPS). In addition, the peaks representing humic-like substances showed a blue shift, indicating that SDS had the greatest effect on SL-EPS. This study provides a guidance for refining the mechanism of strain EPS response to reservoir residual surfactant SDS, and provides a more comprehensive and in-depth understanding of surfactant-protein interactions.

The telltale fluorescence fingerprints of sewer flows for interpreting the low influent concentration in wastewater treatment plant

Low degradability of wastewater treatment plant (WWTP) influents negatively affects its ability to effectively remove pollutants through wastewater treatment processes. Proactive assessment of urban sewer system performance is highly valued in the selection of targeted countermeasures for this occurrence. In this study, a fluorescence spectrum interpretation approach was developed to identify the causes of low biodegradability of WWTP influent by using parallel factor analysis (PARAFAC) and fluorescence regional integration (FRI) of excitation-emission matrix spectroscopy. Statistical analysis was also used to further interpret the PARAFAC- and FRI-derived data. The urban sewer catchment served by a WWTP in Wuhan City, China, was used as the test site to demonstrate the effectiveness of this approach. The results showed that electronics manufacturing industrial wastewater and groundwater input into the urban sewer would significantly decrease the biodegradability of the WWTP influents, and these sources were characterized by much lower fluorescence peak intensities, especially for protein-like substances, including tryptophan-like T and tyrosine-like B1 and B2. The potential conversion of high freshness T into low freshness B2 within the sewer may also contribute to this undesirable scenario. The ratio of peak T to peak B2 and the ratio of the FRI fraction of region I to that of region II can be used together to determine the predominance of industrial wastewater and groundwater. T/B2 < 1.3 indicates the entry of industrial wastewater or groundwater into urban sewers, and I/II > 0.5 further confirms the input of industrial wastewater. Accordingly, the low biodegradability of the WWTP influents in our study site is mostly due to the inflow of industrial wastewater rather than groundwater infiltration into the urban sewers. Therefore, actions should be focused on the surveillance of industrial wastewater rather than widespread sewer inspection and repairs. In this way, this methodology is cost-effective in aiding targeted countermeasures to improve the urban sewer system performance.

Responses of partial denitrification process to long-term perfluorooctane sulfonic acid stress: Performance, microbial community and functional genes

This study investigated the changes in reactor performances, microbial communities and functional genes in partial denitrification (PD) process under elevated perfluorooctane sulfonic acid (PFOS) stress. The results showed that PFOS significantly (P ≤ 0.001) decreased NO-2-N accumulation rate from 71.15 ± 1.83 % to 65.96 ± 3.97 % and increased total nitrogen removal efficiency from 25.49 ± 1.67 % to 30.90 ± 4.97 %, which indicated that PFOS addition hindered the PD process but promoted the total nitrogen removal. Excitation emission matrix fluorescence spectroscopy and fourier transform infrared spectroscopy suggested significant changes in fluorescence characteristics and functional groups of extracellular polymeric substances, especially, PFOS induced the considerable synthesis of protein-like substances. In addition, high-throughput sequencing revealed that the abundance of genus Thauera, which is responsible for the NO-2-N accumulation, was significantly decreased from 35.98 % to 26.00 % under PFOS stress. While the abundances of common denitrifying bacteria, such as genus Denitratisoma, SM1A02 and OLB8, increased after PFOS exposure, which was consistent with the changes of nitrogen transition. Moreover, PICRUSt showed that the abundance of NO-3-N reductase genes (napA) significantly (P ≤ 0.001) downregulated from 0.02 % to 0.01 %, while NO-2-N reductase genes (nirK) significantly (P ≤ 0.001) upregulated by 1.97 folds after PFOS addition. These results showed that PFOS stress had a profound influence on microbial structure and the functional genes. Overall, this study demonstrated the response mechanism of PD system to long-term PFOS stress and made some contributions to the improvement in the control strategy of wastewater treatment plants (WWTPs).

Characteristics of microplastic-derived dissolved organic matter and its binding with pharmaceuticals unveiled by fluorescence spectroscopy and two-dimensional correlation spectroscopy

Microplastics were an extensively detected pollutant in the environment, but microplastic-derived dissolved organic matter (MP-DOM) has received less attention, much less its impact on the binding behavior of pollutants (e.g., pharmaceuticals). In this study, DOM derived from two typical MPs, i.e., polyethylene terephthalate (PET) and polystyrene (PS) was generated by UV irradiation (a widely used way for MPs' aging treatment) and characterized by multiple spectroscopic techniques and methods. Chloramphenicol (CAP) and carbamazepine (CBZ) were selected to investigate the binding mechanism between MP-DOM and pharmaceuticals. After UV irradiation, the concentration of the dissolved organic carbon, colored DOM, and carboxyl/carbonyl groups of MP-DOM increased. Moreover, the humic-like substance released preceding and more under UV irradiation. Furthermore, the protein-like substances on PET-DOM and the humic-like substances on PS-DOM were positively correlated to the binding capacity to the pharmaceuticals, respectively. 2D-COS results revealed that the fluorescent materials having more oxygen-containing functional groups for MP-DOM preferentially interacted with the pharmaceuticals. Overall, the higher fluorescence quenching was related to the protein-like substance, CBZ, and PET-DOM as compared to the humic-like substance, CAP, and PS-DOM. It was verified by the relatively high binding ability (logKM) for them (the protein-like substance: 5.15; CBZ: 4.61; PET: 4.48). This study first proved the environmental reactivity of MP-DOM to the pharmaceuticals highlighting the significance of the spectral properties for the binding behavior of MP-DOM with pharmaceuticals and the competitive sorption role of MP-DOM to the pollutants in the natural environment.

Double-edged sword effects of mechanical aeration on granulation of Spirulina platensis for mariculture wastewater treatment

Microalgae biogranulation holds immense promise for the efficient treatment of high-salinity mariculture wastewater. However, a comprehensive understanding of the underlying mechanisms and critical operational parameters driving microalgae granule formation is crucial for successful practical implementation. In this study, granulation of the salt-tolerant microalgal strain Spirulina platensis was attempted under different aeration intensities to treat synthetic mariculture wastewater. Although all the four reactors at upflow air velocity (UAV) of 0.45, 0.9, 1.35, and 2.70 cm/s, respectively achieved high removals of total nitrogen (85 %), total phosphorus (90 %), and dissolved organic carbon (80 %), S. platensis granules were only formed under UAV of 1.35 cm/s or higher. The granules at UAV 2.70 cm/s doubled alginate-like exopolymers recovery to 113.60 mg/g-MLVSS and P content to 11.86 mg/g-MLSS, whereas the proportion of S. platensis in the microbial community decreased significantly to 4.91 % and energy consumption increased. Mechanism analysis suggested S. platensis granulation commenced with tryptophan- and aromatic protein-like substances, followed by Marinobacter-mediated flocculation and augmented extracellular polymeric substance excretion, and final shaping through shear force. To conclude, this study highlights the dual nature impact of mechanical aeration on S. platensis granulation in mariculture wastewater treatment, and the need for diverse microalgae granulation strategies tailored to specific objectives in practical applications is emphasized.

Deciphering the bioavailability of dissolved organic matter in thermophilic compost and vermicompost at the molecular level

Studies on compost dissolved organic matter (DOM) previously focus on its composition and humification, without considering DOM bioavailability to understand compost fertility. To decipher the fertility basis of compost, DOM bioavailability in thermophilic compost (TC) and vermicompost (VC) was investigated and linked with its molecular composition. Results showed that DOM bioavailability of VC (36 % BDOC) was generally higher than that of TC (22 % BDOC) due to containing more tannin-like substances. Inversely, only lipid-/carbohydrate-/protein-like substances contributed to DOM bioavailability in TC. Moreover, these differences of bioavailability expanded with C/N decreased in composting materials. Specifically, the %BDOC of VC with N-rich materials (C/N < 25) was 2.1–3.0 times higher than that in TC, while it was only 1.2–1.4 times for C-rich materials (C/N < 25), because N-surplus facilitated the formation of O-/N-containing aromatics (e.g., CHON and tannin) in VC, but inhibited the decomposition of organic materials into small bioactive molecules in TC.

Harnessing the composition of dissolved organic matter in lagoon sediment in association with rare earth elements using fluorescence and UV–visible absorption spectroscopy

Dissolved Organic Matter (DOM) plays a pivotal role in influencing metal binding and mobility within lagoon sediments. However, there exists a gap in understanding the compositional alterations of DOM concerning Rare Earth Elements (REEs) across varying pollution gradients. This study aimed to characterize DOM and examine its relationship with REEs in sediment cores from different pollution levels in Yundang Lagoon, China using excitation-emission matrix-parallel factor analysis (EEM-PARAFAC). The results raveled four distinct fluorescent components. Among these, two correspond to humic-like substances, while the remaining two are attributed to protein-like substances. Remarkably, the prevalence of protein-like compounds was observed to exceed 58% of the total fluorescence intensity across all the investigated sites. Furthermore, a substantial discrepancy in total fluorescence intensity was detected between the Songbai Lake and the Inner and Outer Lagoon, indicating a variance in DOM content. In terms of REEs, the average concentration of total REEs was notably elevated within the Songbai Lake sediments (318.36 mg/kg) as compared to the Inner and Outer Lagoon sediments (296.36 and 278.05 mg/kg, respectively). Of significance is the enrichment of Light Rare Earth Elements (LREEs), particularly Ce, La, Pr, and Nd, over Heavy REEs (HREEs) across all surveyed locations. Intriguingly, a coherent trend emerged wherein the fluorescence intensity and LREE concentrations exhibited a synchronized increase from Outer to Inner to Songbai Lake core sediments. This observation substantiates a strong correlation between DOM content and pollution levels (p < 0.05). By shedding light on the intricate interplay between DOM and REEs within urban aquatic sediments, this study imparts novel insights which enrich our comprehension of urban environmental dynamics.

Characteristics and phytotoxicity of hydrochar-derived dissolved organic matter: Effects of feedstock type and hydrothermal temperature

The dissolved organic matter (DOM) with high mobility and reactivity plays a crucial role in soil. In this study, the characteristics and phytotoxicity of DOM released from the hydrochars prepared from different feedstocks (cow manure, corn stalk and Myriophyllum aquaticum) under three hydrothermal carbonization (HTC) temperatures (180, 200 and 220°C) were evaluated. The results showed that the hydrochars had high dissolved organic carbon content (20.15 to 37.65 mg/g) and its content showed a gradual reduction as HTC temperature increased. Three fluorescent components including mixed substance of fulvic acid-like and humic acid-like substances (C1, 30.92%-58.32%), UVA humic acid-like substance (C2, 25.27%-29.94%) and protein-like substance (C3, 11.74%-41.92%) were identified in hydrochar DOM by excitation emission matrix spectra coupled with parallel factor analysis. High HTC temperature increased the relative proportion of aromatic substances (C1+C2) and humification degree of hydrochar DOM from cow manure, while it presented adverse effects on the hydrochar DOM from corn stalk and Myriophyllum. aquaticum. The principal component analysis suggested that feedstock type and HTC temperature posed significant effects on the characteristics of hydrochar DOM. Additionally, seed germination test of all hydrochar DOM demonstrated that the root length was reduced by 8.88%-26.43% in contrast with control, and the germination index values were 73.57%-91.12%. These findings provided new insights into the potential environmental effects for hydrochar application in soil.

Fluorescence spectroscopic characterization of dissolved organic matter in the wastewater treatment plant and hybrid constructed wetlands coupling system in winter: A case study in eastern China

Dissolved organic matter (DOM) in municipal wastewater can have a detrimental effect on aquatic ecosystems, as DOM can participate in the global carbon cycle and change the transport of other pollutants. The characteristics of DOM can also affect the utilization efficiency of dissolved organic carbon by microorganisms in water. In this study, variations in DOM quantity and quality in a full-scale wastewater treatment plant (WWTP) coupled with a hybrid constructed wetland (CW) system during cold periods were characterized by using excitation-emission matrix spectroscopy combined with parallel factor analysis (EEM-PARAFAC). The results showed that the DOM concentration significantly decreased after exiting the WWTP, while a slight increase was found in the CW. The coupled system could significantly eliminate influent DOM with a removal efficiency of 92.1%. The CW system could still remove DOM even in winter. Five fluorescent components, including three humic-like components, one protein-like component and one tyrosine-like component, were identified. Although the biological treatment process in the WWTP contributed a large number of protein-like substances, the optical indices showed that the bioavailability of wastewater was gradually reduced, indicating that DOM was selectively removed by the integrated system. Moreover, there were significant correlations between water quality parameters and DOM indices. This study could contribute to a better understanding of the elimination process of dissolved organic carbon in coupled systems of WWTPs and CWs and offer novel ideas for organic carbon resource recovery and water quality monitoring in watershed environments.

Characterizing water-soluble brown carbon in fine particles in four typical cities in northwestern China during wintertime: integrating optical properties with chemical processes

Abstract. Brown carbon (BrC) aerosol could impact atmospheric radiative forcing and play a crucial role in atmospheric photochemistry. In this study, fine particulate matter (PM2.5) filter samples were collected synchronously in four major cities in northwestern China during the winter season (December 2019–January 2020): Lanzhou (LZ), Xining (XN), Yinchuan (YC), and Ürümqi (UR), which are represented as energy-producing and heavy manufacturing cities in China. The primary aim of the study is to explore the optical properties, sources, and chemical processes of water-soluble BrC (WS-BrC). The average mass absorption efficiency at 365 nm (MAE365) of WS-BrC at these four cities was 1.24 ± 0.19 m2 g−1 (XN), 1.19 ± 0.12 m2 g−1 (LZ), 1.07 ± 0.23 m2 g−1 (YC), and 0.78 ± 0.16 m2 g−1 (UR). The properties of WS-BrC were further investigated by an acid–base titration experiment. The results showed that the MAE365 values in all cities increased with higher pH values (2–11), while the fluorescence intensities of water extracts fluctuated with pH values, being stronger under both highly acidic and basic conditions. The sensitivity to pH variation was most pronounced in the WS-BrC samples from YC and LZ, indicating the important contribution of acid or base functional group compounds in these locations. Additionally, the study revealed significant photo-enhancement (LZ) or photo-bleaching (YC and UR) phenomena of WS-BrC in different cities. These results suggest that the sources and/or chemical processes of WS-BrC varied among the cities. The sources and chemical processes of WS-BrC were further explored by a combination of parallel factor analysis (PARAFAC) on excitation–emission matrix (EEM) spectra of WS-BrC and positive matrix factorization analysis (PMF) on high-resolution mass spectra of water-soluble organic aerosol (WSOA). Six PARAFAC components were identified, including three humic-like substances (HULIS; two less oxygenated (LO) HULIS and one highly oxygenated (HO) HULIS), two protein-like or phenol-like substances (PLS), and one undefined substance. Four PMF factors were identified, including a water-soluble primary OA (WS-POA), a less oxidized oxygenated OA associated with coal combustion-induced WSOA (LO-OOA), and two highly oxidized oxygenated OAs resulting from photochemical oxidation and aqueous-phase oxidation transformations (HO-OOA1 and HO-OOA2). WS-POA was determined to be the most significant source of light absorption, accounting for 30 %–60 % based on multiple linear regression models, and it showed a significant correlation with PLS and LO-HULIS components. The loss of light absorption of WS-POA was found to occur through its conversion to LO-OOA and HO-OOAs through photochemical or aqueous reactions, with HO-OOAs being significantly correlated with the HO-HULIS component. These processes can be clearly illustrated by integrating optical properties and chemical composition using a Van Krevelen diagram and an EEM plot.

Spectroscopic analysis on effluent organic matter (EfOM) evolution in different UV-based advanced oxidation processes

The effluent organic matter (EfOM) removal performance of the advanced wastewater treatment process has drawn great attention owing to the potential ecological risk of EfOM in the receiving aquatic environments. However, the EfOM evolution process is still unclear in the various advanced wastewater treatment processes. In this study, the EfOM evolution process in terms of components, structures, and functional groups was investigated in four kinds of UV-based advanced oxidation processes (AOPs). The overall EfOM removal performances were in the order: UV/NaClO > UV/PMS > UV/H2O2 > UV. According to the spectroscopic analysis results, simple aliphatics/aromatic/acetylenes compounds with C–O, =C–H, amides, or amino structures deriving from protein-like substances, are easily destroyed under UV irradiation. Apart from these structures, microbial metabolic was strongly sensitive to a reactive chlorine species (RCS)-dominated system, especially nutrients, amino acids, and small peptides with ester. Total of 22 species of halogen by-products with high biotoxicity (acetonitrile-based chlorinated EfOM, chlorinated acetate, chlorinated methane) were detected in the UV/NaClO group. In contrast, the reactive oxygen species (ROS)-dominated systems exhibited strong structure selectivity for ester (UV/H2O2) and carbonyl (UV/PMS) in all of the EfOM components, exhibiting strong reaction activity with EfOM at different stages. The results reveal the EfOM evolution in different UV-based AOPs, providing a strategy for selecting suitable UV-based AOPs in the advanced wastewater treatment process in terms of high-efficiency removal of EfOM.

The effect and spectral response mechanism of dissolved organic matter (DOM) in Pb(II) adsorption onto biochar

This study investigated the interaction of biochar-derived dissolved organic matter (DOM) and metal pollutant Pb(II) during adsorption. Research data showed that the adsorption capacity of biochar and residual biochar (after removal of DOM) was 277.62 and 204.47 mg g−1, respectively, indicating that DOM played a significant role in enhancing the adsorption efficiency of Pb(II). The results indicated that the adsorption process of Pb(II) on biochar and residual biochar followed the pseudo-second-order kinetic equation (R2 > 0.99), and the adsorption isotherm was much closer Freundlich model. A combination of three-dimensional excitation-emission matrix (3D-EEM), synchronous fluorescence, Fourier transform infrared spectroscopy (FT-IR), and two-dimensional correlation spectroscopy (2D-COS) was employed to investigate the interaction between DOM and Pb(II). 3D-EEM indicated the presence of four major components of DOM, namely two protein-like substances, one humic acid-like substance, and one fulvic acid-like substance. The synchronous fluorescence revealed a gradual quenching of the fluorescence of the DOM sample with an increase in Pb(II) concentration, indicating a static quenching mechanism between DOM and Pb(II). FTIR-2D-COS analysis showed a specific sequence of DOM binding with Pb(II) is as follows: 1500 > 1010 > 880 > 1390 > 1260 cm−1. This findings have significant implications for the role of DOM in environmental science and have potential applications in various fields.

Photochemical behavior of dissolved organic matter derived from Alternanthera philoxeroides hydrochar: Insights from molecular transformation and photochemically reactive intermediates

Hydrochar-derived dissolved organic matter (HDOM) enters aquatic ecosystems through soil leaching and surface runoff following the application of hydrochar. However, the photochemical behavior of HDOM remains unclear. The photo-transformation of HDOM was analyzed by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), multiple spectroscopy methods, high-performance liquid chromatography, and combining synchronous fluorescence and Fourier-transform infrared spectroscopy with two-dimensional correlation spectroscopy. The results showed that with the increase of carbonization temperature, amide II in protein-like substances were observed to be preferentially photolyzed, and the protein-like substances were more sensitive to low irradiation time, while the duration time of the photochemical behavior of amide II and aliphatic C−H were more persistent. FT-ICR MS results showed that N and S-containing molecules, including lignins and lipids were more sensitive to ultraviolet irradiation. Furthermore, the photo-transformation of HDOMs was accompanied by the generation of triple excited state dissolved organic matter and singlet oxygen. Our findings will be beneficial for understanding the mechanisms of photo-transformation of HDOM and for predicting the possible behaviors of hydrochar produced at different temperatures before large-scale application.

Enhanced membrane fouling by microplastics during nanofiltration of secondary effluent considering secretion, interaction and deposition of extracellular polymeric substances

Microplastic (MP) has been found to influence membrane fouling during microfiltration/ultrafiltration processes in direct and indirect ways by acting as fouling components and changing microbial activities, respectively. However, there is no relevant research about the contribution of MPs to nanofiltration membrane fouling. In this study, for the first time, the impacts of MPs on membrane fouling during the nanofiltration of secondary effluent (SE) were systematically investigated from the perspective of bacterial extracellular polymeric substances (EPS) secretion, their interaction with coexisting pollutants and also deposition. Membrane flux behaviors indicate that MPs simultaneously aggravated the short-term and long-term membrane fouling resistance of nanofiltration by 46 % and 27 %, respectively. ATR-FTIR, XPS and spectrophotometry spectra demonstrate that the deteriorated membrane fouling by MPs directly resulted from the increased accumulation of protein-like, polysaccharides-like and humic-like substances on membranes. EEM spectra further confirmed that MPs preferred to induce serious cake layers, which dominated membrane flux decline but hindered pore fouling. According to CLSM and SEM-EDS mappings, MPs in SE could stimulate microbial activities and then aggravate EPS secretion, after which their interaction with Ca2+ was also enhanced in bulk solution. The cross-linker nets could promote the deposition of other unlinked pollutants on membranes. Besides, MPs could weaken the rejection of certain dissolved organic matters (from 57 % to 52 % on the 50th day of filtration) by aggravating cake-enhanced concentration polarization (CECP), but improved the average removal of inorganic salts from 58 % to 63 % by improving their back diffusion through cake layers. Based on these analyses, the mechanisms of MP-enhanced membrane fouling during the nanofiltration of SE can be thoroughly revealed.

Copper regulates degradation of typical antibiotics by microalgal-fungal consortium in simulated swine wastewater: insights into metabolic routes and dissolved organic matters

Microalgae-based biotechnology for antibiotics biodegradation in swine wastewater has been receiving an increasing attention. In this study, microalgae and fungi co-cultivation system, regulated by copper (Cu(II)), was investigated in terms of nutrients and sulfonamides degradation in simulated swine wastewater. Results showed that the removal of ammonium nitrogen (NH4+-N), total nitrogen (TN), total phosphorus (TP) and chemical oxygen demand (COD) by microalgal-fungal consortium increased under 0.1-0.5 mg/L Cu(II) with the highest removal efficiency of 79.19%, 76.18%, 93.93% and 93.46%, respectively. The addition of Cu(II) (0-0.5 mg/L) enhanced the removal of sulfamonomethoxine (SMM), sulfamethoxazole (SMX) and sulfamethazine (SMZ) from 49.05% to 58.76%, from 59.31% to 63.51%, and from 37.51% to 63.9%, respectively, and the main removal mechanism was found to be biodegradation. Biodegradation followed a pseudo-first-order model with variable half-lives (10.12 to 15.51 days for SMM, 9.01 to 10.88 days for SMX, and 8.74 to 12.85 days for SMZ). Through mass spectrometry analysis, metabolites and intermediates of sulfonamides were accordingly identified, suggesting that the degradation routes were involved with hydroxylation, deamination, oxidation, de-sulfonation and bond cleavage. Dissolved organic matters released by microalgal-fungal consortium were induced by Cu(II). Fulvic acid-like and protein-like substances were bound to Cu(II), reducing its concentration and thus mitigating the organismal damage to microorganisms. These findings drew an insightful understanding of microalgal-fungal consortium for sulfonamides remediation by Cu(II) regulation in simulated swine wastewater.

Halophyte Elymus dahuricus colonization regulates microbial community succession by mediating saline-alkaline and biogenic organic matter in bauxite residue

Alkalinity regulation and nutrient accumulation are critical factors in the construction of plant and microbial communities and soil formation in bauxite residue, and are extremely important for sustainable vegetation restoration in bauxite residue disposal areas. However, the establishment and succession of microbial communities driven by plant colonization-mediated improvements in the physicochemical properties of bauxite residues remain poorly understood. Thus, in this study, we determined the saline-alkali properties and dissolved organic matter (DOM) components under plant growth conditions and explored the microbial community diversity and structure using Illumina high-throughput sequencing. The planting of Elymus dahuricus (E. dahuricus) in the bauxite residue resulted in a significant decrease in total alkalinity (TA), exchangeable Na, and electrical conductivity (EC) as well as the release of more tryptophan-like protein compounds and low-molecular-weight humic substances associated with biological activities into the bauxite residue substrate. Taxonomical analysis revealed an initial-stage bacterial and fungal community dominated by alkaline-tolerant Actinobacteriota, Firmicutes, and Ascomycota, and an increase in the relative abundances of the phyla Bacteroidota, Cyanobacteria, Chloroflexi, and Gemmatimonadota. The biological activities of phylum Actinobacteriota, Bacteroidota, and Gemmatimonadota were significantly associated with protein-like and UVA-like humic substances. As eutrophic bacteria, Proteobacteria participate in the transformation of humic substances and can not only utilize small molecules of organic matter and convert them into humic substances but also promote the gradual conversion of humic acids into simple molecular compounds. Our results suggest that plant roots secrete organic matter and microbial metabolites as the main biogenic organic matter that participates in the establishment and succession of the microbial community in bauxite residues. Root length affects bacterial and fungal diversity by mediating the production of protein-like substances.