Photochemical behavior of dissolved organic matter derived from Alternanthera philoxeroides hydrochar: Insights from molecular transformation and photochemically reactive intermediates

Abstract

Hydrochar-derived dissolved organic matter (HDOM) enters aquatic ecosystems through soil leaching and surface runoff following the application of hydrochar. However, the photochemical behavior of HDOM remains unclear. The photo-transformation of HDOM was analyzed by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), multiple spectroscopy methods, high-performance liquid chromatography, and combining synchronous fluorescence and Fourier-transform infrared spectroscopy with two-dimensional correlation spectroscopy. The results showed that with the increase of carbonization temperature, amide II in protein-like substances were observed to be preferentially photolyzed, and the protein-like substances were more sensitive to low irradiation time, while the duration time of the photochemical behavior of amide II and aliphatic C−H were more persistent. FT-ICR MS results showed that N and S-containing molecules, including lignins and lipids were more sensitive to ultraviolet irradiation. Furthermore, the photo-transformation of HDOMs was accompanied by the generation of triple excited state dissolved organic matter and singlet oxygen. Our findings will be beneficial for understanding the mechanisms of photo-transformation of HDOM and for predicting the possible behaviors of hydrochar produced at different temperatures before large-scale application.