Poly Herbal Research Articles

Efficacy and Safety of Iranian Poly Herbal Formulation (Compound Honey Syrup) in Pediatric Patients with Mild to Moderate Asthma: A Randomized Clinical Trial

Background: Asthma is a chronic relapsing airways disease that represents a major public health problem worldwide. With the high incidence of asthma, there has been a surge in the use of complementary therapies, such as compound honey syrup in Traditional Persian Medicine, in the treatment of asthma. The aim of this study was to evaluate the efficacy and safety of Iranian poly herbal formulation (compound honey syrup) in the treatment of mild to moder­ate pediatric asthma. Materials and Methods: The study was a randomized clinical trial that was conducted on 80 patients with mild to moderate asthma assigned to two groups (n=40 for each group) for eight weeks. Control and experimental groups received classical treatment of asthma with fluticasone spray; in case of worsening of symptoms, salbutamol spray was used for short term. The experimental group also received compound honey syrup (the combina­tion of honey and an extract of the following five medicinal plants: ginger, cinnamon, saf­fron, cardamom, and galangal). Asthma Control Questionnaire (ACQ) items and total scores of ACQ were evaluated before and after treatment. Results: To this end, 72 patients complet­ed this study. There was no significant difference between the experimental and the control groups in baseline data such as age, sex, body mass index, ACQ items, and ACQ scores. Total scores and all items of ACQ, with the exception of forced expiratory volume in one second (FEV1%), were significant between groups (P<0.05). No serious adverse effects were ob­served in the two groups. Conclusions: The results of this study reveal that compound honey syrup can be a safe and effective complementary drug for the treatment of pediatric asthma [GMJ.2017;6(4):291-301] DOI: 10.22086/gmj.v6i3.884

Phytoestrogenic effect of Inula racemosa Hook f – A cardioprotective root drug in traditional medicine

Ethnopharmacological relevanceRoots of Inula racemosa are used as a cardio protective in Ayurveda in India, being prescribed as a medicine for precordial chest pain, cough and dyspnoea, both singly and as a poly herbal. AimEvaluation of Phytoestrogenic activity of the root extracts of Inula racemosa and compounds isolated therefrom in vivo, in silico and in vitro. Materials and methodsAlcohol (IrA) and hexane (IrH) extracts characterized by HPTLC/GC-MS analysis respectively and processed for compound isolation were evaluated for estrogenic activity (100 & 250mg/kg bw) by the Immature rat uterotrophic assay using ethinylestradiol (EE −30µg/kg bw) as standard drug. Alantolactone (ALT), Isoalantolactone (IALT) and Stigmasterolglucoside (SG) isolated from the extracts were characterized and screened in silico for ERα, ERβ binding affinity, assessed in vitro for growth modulatory effects on MCF-7 cells by MTT assay and cell cycle distribution analysis using Flow cytometry. RT-PCR analysis evaluated the mRNA expression of pS2 in these cells post exposure to ALT, IALT and SG. ResultsIn the IrA treated groups there has been a statistically significant increase (P < 0.05) in absolute and normalised uterine weight, uterine diameter, endometrial thickness, luminal epithelial cell height,diameter of ovary and in the number of primary and secondary ovarian follicles relative to untreated controls. Presence of ciliated epithelial cells in the oviduct, elevated number of early growing follicles characterized by an increased oocyte diameter, and signs of vascularization in the cortex of ovarian sections in this group relative to EE treated group are indicative of pervasive activation of follicular growth and initiation. Virtual docking demonstrated ERα affinity for IALT, ERβ affinity for ALT, while SG showed a high binding affinity to both with a relatively greater ERβ binding affinity. Dose dependent decrease in cell viability mediated by IALT and SG in the MTT assay is corroborated by a statistically significant increase (p < 0.05) in sub G0-G1 cells by SG at 200 and 400µM in cell cycle analysis and there has been an induction of pS2 by IALT and SG in the ER regulated MCF-7 cells. ConclusionsDemonstration of classical morphological changes induced by estrogen stimulation mediated by IrA in vivo at both the tested doses, isolation of the antioxidant SG from IrA and its dose dependent growth inhibitory effect on estrogen sensitive MCF-7 cells through apoptotic induction and an up regulation of pS2 are suggestive of an anti-estrogenic effect through estrogen receptor binding affinity, typical of phytoestrogens that bind to ER but do not elicit a full estrogenic response. The observed estrogenic effect of IrA suggests a multi mechanistic molecular action involving antioxidant as well as redox signalling pathways acting in consonance with their anti-estrogenic effects owing to the weak estrogen like competitive receptor binding of SG.

Development and Evaluation of Poly Herbal Molluscicidal Extracts for Control of Apple Snail (Pomacea maculata)

Golden Apple Snail (GAS) is the most destructive invasive rice pest in Southeast Asia. The cost of synthetic molluscicides, their toxicity to non-target organisms, and their persistence in the environment have propelled the research of plant-derived molluscicides. Most research efforts have focused on individual plant extracts for their molluscicidal potency against GAS and have not been proven to be entirely effective in rice field conditions. Selective combination of synergistically acting molluscicidal compounds from various plant extracts might be an effective alternative. In this direction, ethanolic extracts from six different plants (Neem, Tobacco, Nerium, Pongamia, Zinger, and Piper) were evaluated against Pomacea maculata Perry. Of the various combinations studied, a binary extract (1:1) of nerium and tobacco (LC90 177.71 mg/L, 48 h), and two tri-herbal extract formulations (1:1:1) of (nerium + tobacco + piper) and (nerium + tobacco + neem) were found to be most effective, with LC90 values of 180.35 mg/L and 191.52 mg/L, respectively, in laboratory conditions. The synergistic effect of combined herbal extracts resulted in significant reduction in LC90 values of the individual extracts. The findings of this study demonstrate that the selective combinations of potent molluscicidal herbal extracts are effective for management of P. maculata under laboratory conditions.

HPTLC Marker-based Analysis of Madhuram, a Pre-diabetic Proprietary Poly Herbal

Proper validation of traditional medical knowledge using current scientific techniques and reasoning is vital before complementary and alternative medicinal products are integrated into conventional clinical practice. The present study was designed to standardize a proprietary Ayurvedic polyherbal preparation, Madhuram. Concocted as a mixture of six popular antidiabetic herbs as per Ayurvedic therapeutics, this is being prescribed as a health supplement in prediabetes. Capsule contents were processed for HPTLC analysis based on markers phyllanthin, berberine, ellagic acid, gallic acid, gymnemic acid and sennoside B through sample enrichment and were quantified against reference standards. The retention factors were phyllanthin 0.35±0.03, berberine 0.32±0.02, gallic acid 0.41±0.02, ellagic acid 0.29±0.04, gymnemic acid 0.36±0.01 and sennoside B 0.41±0.01 in their respective solvent systems. The peaks for phyllanthin, berberine, gallic acid, ellagic acid, gymnemic acid in their respective chromatograms of the formulation authenticated the raw material. Sennoside B was not detected in its enriched fraction of sample. Quantitation by densiometric scanning followed by peak integration demonstrated the presence of 3.17 µg of phyllanthin, 4.92 µg of berberine, 72 µg of gallic acid, 66 µg of ellagic acid, 410 µg of gymnemic acid. The evaluation of antidiabetic poly herbal established the identity of label claim herbs. Quantification of markers characteristic of each herb has laid the groundwork for batch to batch standardization to check its content uniformity.

Treatment of Mycotic Dermatitis in Domestic Animals with Poly Herbal Drug

Treatment of Mycotic Dermatitis in Domestic Animals with Poly Herbal Drug

A Wound Healing Formulation Based on Iranian Traditional Medicine and Its HPTLC Fingerprint.

Herbal therapy was the common treatment prescribed by Iranian physicians for wound healing. "Zemad" was the most ancient pharmaceutical dosage form used in Iranian Traditional Medicine (ITM) for skin diseases. In the present research, formulation of a traditionally used Poly Herbal Paste (PHP) for wound healing was performed. Moreover, the fingerprint of the product was prepared by HPTLC method for identification and quality assessment of the formulation. 3.33 % of each plant materials containing Aloe vera, Boswellia carteri and Commiphora myrrha were used in a hydrophilic base. The physical stability and rheological behavior of the formulation was evaluated. Moreover, microbiological tests was performed. Methanol fraction of A. vera, C. myrrha and B. carterri were used as standard materials in HPTLC method. Stability and rheological behavior evaluations as well as microbiological tests showed that the prepared formulation was stable towards physical changes with no growth of pathogenic microorganisms and suitable for topical application. HPTLC fingerprinting of PHP confirmed the presence of compounds corresponding to the plants used in the formula. Regarding to the antioxidant, anti-inflammatory and antimicrobial effects of the constituents of PHP, the product could be an appropriate candidate for wound healing with respect to its traditional use in ITM. In addition, HPTLC fingerprinting could be used as an applicable method for quality control assessment of the prepared formulation.

Comparative Evaluation of Poly Herbal Formulation from Traditionally Used Eritrean Medicinal Plants against Pathogenic Microorganisms

Comparative Evaluation of Poly Herbal Formulation from Traditionally Used Eritrean Medicinal Plants against Pathogenic Microorganisms

Free radical scavenging activity of Shirishavaleha - A Poly Herbal Ayurvedic formulation

Objective: Synthetic antioxidants such as butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA) have recently been reported to be hazardous to human health. Thus, the search for effective, non-toxic natural compounds with antioxidative activity has been intensified in recent years. The current attempt aimed to determine the antioxidant activity of two samples of Shirishavaleha, one prepared with water and the other with Kanji as liquid media, using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and lipid peroxidation (LPO) assay. Methods: Batches of Shirishavaleha with water and Kanji were prepared by following standard manufacturing procedures. Quercetin (2 mg/ml) and butylated hydroxyanisole (BHA, 0.8 mg/ml) were used as standard drugs for DPPH and LPO, respectively. Results: Both the drugs were found to present clear DPPH radical and LPO scavenging activity, comparatively at higher degrees in the sample prepared with Kanji. Conclusion: Based on the observations; it can be concluded that both the samples possesses significant antioxidant activities which may open their way for being used as potential natural antioxidants.

Wound Healing Activity of a Traditionally Used Poly Herbal Product in a Burn Wound Model in Rats.

Background:Burns are known as one of the most common and destructive forms of injury with a vast spectrum of consequences. Despite the discovery of various antibacterial and antiseptic agents, burn wound healing still has remained a challenge to modern medicine. Plants, with a valuable traditional support, have been considered as potential agents for prevention and treatment of disorders in recent years. However, modern scientific methods should be applied to validate the claims about the therapeutic effects of the herbal products.Objectives:This study was conducted to evaluate the wound-healing activity of a poly herbal cream (PHC), retrieved from Iranian Traditional Medicine (ITM), in a rat burn wound model in Iran.Materials and Methods:In this experimental study, PHC containing aqueous extracts of Malva sylvestris and Solanum nigrum leaves and oily extract of Rosa damascena petals was used. Second-degree burn wounds were induced in four groups of five rats each. Group 1 received no treatment while groups 2, 3 and 4 were given cream base, silver sulfadiazine (SS) 1% and PHC, respectively to compare the efficacy of PHC with the negative and positive control groups. The percentage of wound healing on days 2, 6, 10 and 14 and histopathological parameters of healed wounds on the 14th day were assessed. Antioxidant and antimicrobial activities of PHC were evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and micro-dilution methods, respectively.Results:There was a significant improvement in healing percentage of PHC-treated rats in comparison to the other groups at the end of the treatment period (87.0% ± 2.1% for PHC in comparison to 32.2% ± 1.6%, 57.0% ± 5.3% and 70.8% ± 3.5% for the control, cream base and SS groups, respectively). Moreover, the healed wounds in PHC-treated animals contained less inflammatory cells and had desirable re-epithelialization with remarkable neovascularization. In addition to the antioxidant activity, PHC exhibited antibacterial effect against Staphylococcus aureus.Conclusions:Poly herbal cream experimentally and histopathologically revealed a burn wound healing activity probably due to the antioxidant, anti-inflammatory and antimicrobial activities of its phytochemical contents. Therefore, this study confirms the use of M. sylvestris, S. nigrum and R. damascena in burn prescriptions in ITM.

Design, Development and Phytochemical Evaluation of a Poly Herbal Formulation Linkus Syrup

The present work has been carried out on polyherbal formulation named as Linkus Syrup. The herbal formulation consists of Glycyrrhiza glabra, Hyssopus officinalis, Piper longum and Alpinia galangal. The Linkus syrup physico-chemical evaluation such as pH, density, identification of polysaccharide, tanning agents, ascorbic acid and shelf life was complied. The TLC and quantitative determination of alkaloid were quantified. Determination of biomarker has been validated for the analysis of vasicine. The study result revealed that Linkus syrup formulation was well standardized at different levels such as physic-chemical consistency and assay of bio marker compound.

Formulation and Evaluation of Poly Herbal Formulation against CCl4 Induced Hepatotoxicity

Poly Herbal Formulation | A. Marmelos | P. Amarus and E. Alba Carbon Tetra Chloride | Hepatotoxicity | المستخلص العشبي Polyherbal | الكلوريد الكربوني | الألانين ألانين ( ALT ) | الألانين إسبارتاتي ( AST )

Abstract 501: Poly Herbal Formulation Prevents Foam Cell Formation Possibly Through Attenuation of CD36 Scavenger Receptor Signalling Cascade

Objective CD36 signalling cascade promotes migration, spreading and entrapment of lipid laden foam cells within intimal lesions by oxidative stress, inflammation and oxidized LDL (oxLDL) by activation of actin polymerization, Focal Adhesion Kinase (FAK) and cement adhesion which also are indirectly activated by Reactive Oxygen Species (ROS). There is dearth of information about an antagonistic therapy for this CD36 cascading pathway to prevent atherosclerosis. Therefore, we hypothesized that, an antioxidant, antiinflammatory and antihyperlipidemic Poly Herbal Formulation (PHF) consisting of Allium sativum, Zingiber officinale, Curcuma longa, Terminalia arjuna and Cyprus rotundus prevents atherosclerosis. Methods Standardized (WHO and IP monographs) hydro-alcoholic extracts of the above said test materials were mixed in equal amounts to prepare PHF. After conforming the antioxidant, antiinflammatory and antihyperlipidemic activities, PHF was evaluated for foam cell formation and the protein expression of CD36, PPAR-γ and FAK in oxLDL treated mouse macrophage J774A.1 cells. Its inhibitory activity for ROS generation was evaluated. Antiatherosclerotic activity was studied in high cholesterol fed rabbit model. Results Significant accrual of lipid in oxLDL treated cells conform the foam cell formation where CD36, PPAR-γ and FAK protein levels had increased. PHF (20 to 80 μg/mL) prevented lipid accumulation in oxLDL treated cells from ~29% to ~38% where CD36, PPAR-γ and FAK protein levels were significantly decreased in concentration dependent manner up to 3, 2 and 2 folds, respectively. ROS generation was inhibited by PHF in concentration dependent manner. PHF dose of 100 and 200mg/Kg, significantly reduced plasma cholesterol, ~23% and ~40% and plasma triglycerides ~87% and ~91%, respectively. Plasma TBARS were significantly reduced by PHF 100 and 200mg/Kg, 50% and ~66%, respectively. Aortic atheroma formation was significantly barred by PHF as observed in histopathological studies. Conclusion PHF prevents atherosclerosis by preventing foam cell formation possibly by reducing CD36, PPAR-γ and FAK proteins and ROS generation. However, detailed pharmacodynamic studies are needed to correlate these findings in a clinical set up.

A Study on Ayurveda Poly Herbal Compound of Yogaratnakar (17 - A.D.) w.s.r. Rakta-Pradara (Abnormal Uterine Bleeding)

Rakta Pradara (Abnormal uterine bleeding) is commonest problem encountered by gynecologist in today's practice and incidence becomes higher with degree of civilization. Various treatments prescribed in modern medicine like HRT, Antifibrinolytic agents etc have not proved their definite efficacy Inspite of high price and side effect. The present study titled "Clinical study of Shatavari Yashti yoga on Rakta-Pradara w.s.r. to Yogaratnakar " was aimed to observe the efficacy of the trial drug Shatavari Yashti Yoga (Poly herbal compound) in Rakta Pradara (Abnormal uterine bleeding) . The clinical study was conducted on 30 patients selected randomly and divided in to three groups based on the drug for the clinical trial. Group 1- Shatavari Yashti Yoga with Anupana Tandulodaka; Group 2 - Shatavari Yashti Yoga without Anupana and Group 3 - Placebo with Tandulodaka. The Clinical assessment was done on the basis of grading criteria with specific Symptomology of Rakta Pradara (Abnormal uterine bleeding) like Amount and duration of bleeding, intermenstrual bleeding, Pain, burning sensation, pallor, and weakness then mean scores levels of these Symptoms before and after the treatment of all three groups were subjected for student paired 't' test for statistical analysis. Conclusion: The results were statistically and clinically significant not only to cure Rakta Pradara but also in improving general health of women.

Phytochemical Screening and in vitro Antioxidant Activity of Jawarish Amla - A Poly Herbal Formulation

The present research work was carried out to study the antioxidant potential of methanolic extract of Jawarish amla using various in vitro tests including reducing power assay, Superoxide anion scavenging activity assay, Hydroxyl radical scavenging activity assay, nitric oxide scavenging activity assay, DPPH free radical scavenging assay, and hydrogen peroxide method. these various antioxidant activities were compared to standard ascorbic acid. Preliminary phytochemical screening revealed that the extract of jawarish amla possesses flavonoids, steroids and phenolic compounds. In this study, quantitative determinations of flavonoids and phenols were conducted by colorimetric methods, using aluminum chloride method and Folin ciocalteu reagent respectively. the results indicated that methanolic extract of Jawarish amla possess antioxidant property.

Hypoglycemic and hypolipidemic effect of Allopolyherbal formulations in streptozotocin induced diabetes mellitus in rats

Aim of the studyIn the present study, we examined and compared the effect of Polyherbal (PH), Allopolyherbal-A (APH-A), Allopolyherbal-B (APH-B), and Allopolyherbal-C (APH-C) formulations on hyperglycemia, lipid profile, renal, and hepatic function in streptozotocin (STZ) induced diabetes mellitus in rats. Materials and methodsThe hypoglycemic activity (along with other parameters) of Polyherbal and Allopolyherbal formulations was investigated in STZ induced diabetes in rats. Polyherbal (PH) (3.63g/kgbodywt.); Allopolyherbal-A (APH-A) [(5mgGliclazide+1.81gPH)/kgbodywt.]; Allopolyherbal-B (APH-B) [(4mgGliclazide+2.17g of PH)/kgbodywt.]; Allopolyherbal-C (APH-C) [(2mg of Gliclazide+2.904g of PH)/kgbodywt.], and Gliclazide (10mg/kgbodywt.) were administered once a day, orally by gavages for 21days.Blood glucose levels were measured on 0, 7, 14, and 21days of the study; total cholesterol, triglycerides, LDL, VLDL, HDL, serum creatinine, SGOT, and SGPT were estimated on 21st day. ResultsGliclazide, Polyherbal (PH), Allopolyherbal-A (APH-A), Allopolyherbal-B (APH-B), and Allopolyherbal-C (APH-C) formulations treated rats showed significant (P<0.01) decrease in blood glucose, total cholesterol, triglycerides, LDL, VLDL, serum creatinine, SGOT, and SGPT level, along with significant increase in HDL. ConclusionsPresent findings provide experimental evidence that the combination of allopathic hypoglycemic drugs with hypoglycemic Polyherbal formulations provides effective and rapid glycemic control and can also minimize the cardiovascular risk factors of type II diabetes mellitus.

Protective Effect of Br-16a (Poly Herbal Preparation) on the Behavioural and Biochemical Alterations in Sleep Disturbed Mice (Grid Suspended over Water Method)

Rationale: Sleep deprivation disrupts various vital biological and metabolic processes that are necessary for physical health of a human being. Benzodiazepines are the most frequently prescribed drugs for sleep related problems in spite of their side effects such as tolerance and dependence. Objective: The present study was performed to explore the protective effect of BR-16A in sleep-disturbed mice. Material and Method: Male Laca mice (n=6–9/group) were sleep deprived for 48 hour using grid suspended over water method. BR-16A was administered orally for five days (starting three days before sleep deprivation for 48 hours). All the behavioural parameters (locomotor and anxiety) and biochemical tests (lipid peroxidation, reduced glutathione, nitrite and catalase) were performed on 5 th day. Results: 48 hours sleep deprivation significantly decreased body weight, locomotor activity and produced severe anxiogenic response in animals. Besides, significant oxidative stress was also observed in the brain tissues of the animals. Pre treatment of BR-16A (100 mg/kg and 200 mg/kg) and diazepam (0.5 mg/kg) significantly protected reduction in body weight, locomotor activity and improved anxiogenic response. Biochemically, BR-16A (100 mg/kg and 200 mg/kg) and diazepam (0.5 mg/kg) decreased lipid peroxidation level, nitrite levels and improve the antioxidants defences activity as indicated by catalase, reduced glutathione enzyme activities. However, co-administration of BR-16A (100 mg/kg) and diazepam (0.5 mg/kg) could not produce any significant effect on both behaviour as well as biochemical parameters. Conclusion: BR-16A has protective action against sleep deprivation induced behavioural and biochemical alterations. doi: 10.5214/ans.0972.7531.2006.130401

Protective Effect of Br-16a (Poly Herbal Preparation) on the Behavioural and Biochemical Alterations in Sleep Disturbed Mice (Grid Suspended over Water Method)

Protective Effect of Br-16a (Poly Herbal Preparation) on the Behavioural and Biochemical Alterations in Sleep Disturbed Mice (Grid Suspended over Water Method)

Anti-diabetic Activity of SMK001, a Poly Herbal Formula in Streptozotocin Induced Diabetic Rats: Therapeutic Study

The therapeutic anti-diabetic effect of SMK001, a poly herbal formula was evaluated in the streptozotocin (STZ; 60 mg/kg, single intraperitoneal injection) induced diabetic rats. For therapeutic study, test articles were orally dosed once a day from 21 d after STZ-dosing at 100, 200 and 500 mg/kg/5 ml dosage levels for 4 weeks. The body weight changes, blood and urine glucose level changes were monitored with changes on the pancreas weight, and after sacrifice, the histopathological changes of pancreas and the changes of insulin- and glucagon-producing cells were also observed by immunohistochemistry. The results were compared to that of glibenclamide 5 mg/kg-dosing group. Significantly (p<0.01 or p<0.05) decrease of body weight, blood and urine glucose levels were detected in STZ-induced diabetic animals with disruption and disappearance of pancreatic islets. In addition, significantly (p<0.01) increase of glucagon- and decrease of insulin-producing cells were detected in STZ induced diabetic rats. However, these diabetic changes were significantly (p<0.01 or p<0.05) and dose dependently decreased in SMK001-dosing groups, and SMK001 100 mg/kg showed more favorable effects compared to that of glibenclamide 5 mg/kg. Based on these results, it is considered that SMK001 has favorable effect to inhibit the changes on the blood and urine glucose levels, body weight and the histopathological changes of pancreas in STZ induce diabetes.

Effect of a Poly Herbal Ingredient on Day Old Chick Quality by Feeding in Parent Flocks

A trial was undertaken in 99 numbers of white leghorn layer parent stock to study the indirect effect of stresroak (a poly herbal feed additive) on overall chick quality improvement, seven day mortality and maternal antibody transfer to chicks. The stresroak feed mix was given @ 2kg per ton of feed and water supplement @ 20ml / day for 100 birds for one month period. The chicks were received two weeks after supplementation at weekly interval for 5 weeks period to assess the chick quality. The results showed that the chick quality scores of the stresroak supplemented groups were highly significantly different from control group (P<0.01). The first week mortality recorded for the three treatment groups revealed a highly significant difference (P<0.01) upon comparison with control group. The maternal antibody against Ranikhet disease in the stresroak supplemented groups showed a highly significant difference (P<0.01) in the transfer of maternal antibody when compared to control group.