Poly Herbal Research Articles

In Silico Docking Analysis of Poly Herbal Formulation Aadathodai Kudineer used in Siddha medicine in inhibiting Main Protease and ACE2 Receptor Spike protein SARS-CoV-2

Corona virus disease (COVID-19) is an infectious pandemic disease caused by the newly discovered novel corona virus. World Health Organization has declared the global health emergency due to COVID19 outbreak. Currently, there is no specific treatment or vaccine for fighting against this infectious disease. Aadathodai Kudineer is a drug indicated for Iya Erumal, Kozhai Kattu, Kabasuram. Upon the mortality and severity of the disease COVID19, we tried to identify the possible inhibition of phytocomponents of Aadathodai Kudineer in inhibiting Main Protease and ACE2 Receptor Spike protein SARS-CoV-2 through molecular docking studies. Methodology: In Silico molecular docking analysis was performed for phytocomponents present in the Aadathodai Kudineer formulation for targets main protease and ACE2 Receptor Spike protein, PDB ID: 6LU7 and PDB ID: 2AJF using Autodock tool. ADME properties was also predicted for all the above compounds. Results: Among the 9 active Phytocompounds present in the Aadathodai Kudineer formulation, Lupeol showed high binding affinity with COVID19 main protease and ACE2 receptor which shows the promising contrivance of protease inhibition. The ADME suggested that the formulation is free from toxic. Conclusion: The phytocomponents showed possible affinity towards these targets and has the lead molecules that inhibits COVID19 main protease and ACE2 receptor.

Formulation and Finger Printing of a Poly Herbal Film-Coated Tablet for Treatment of Hemorrhoids

Background and objectives: Hemorrhoids is the most prevalent rectal disease. Despite different medical efforts, its complications are not managed well. In the present research, a popular prescription for treatment of hemorrhoids was formulated as tablet dosage form and, its HPTLC fingerprint prepared. Methods: Commiphora mukul was dissolved in Allium ampeloprasum juice (1:3). Then, this solution was blended with Terminalia chebula, Phyllantus emblica and Terminalia bellirica (1:1:1) powder. Different formulations were prepared from the mixture and the best one was selected for tablet preparation. Subsequently, the tablets were coated and their physicochemical characteristics and fingerprint pattern were obtained using silica gel plate, NP/PEG reagent and toluene: ethyl acetate: formic acid (70:15:15) as mobile phase. Laboratory stability studies were carried out as well. Results: Formulation C revealed excellent results in flowability studies (angle of repose: 26, carr’s index: 6, hausner ratio: 1.00). It was also demonstrated acceptable results in different tests including weight variation (500 mg), hardness (8.04 kg/cm2), disintegration time (28.50 min), friability (0.6%), dissolution (97.6% phenolics and 96.1% tannins, respectively) and the coating process. Total phenolics and tannins contents were determined as 125.8 mg/tab and 89.2 mg/tab, respectively. In fingerprinting study, characteristic spots of each species were distinguished. The film-coated tablets were stable in laboratory stability test. Conclusion: With reference to anti-inflammatory, astringent and wound healing roles of phenolics and tannins in hemorrhoids, the present tablets could be an appropriate candidate for hemorrhoids regarding its historical backgrounds.

Physiochemical and Preliminary Phytochemical Screening of Venpoosani Lekiyam – A Poly Herbal Siddha Formulation

Physiochemical and Preliminary Phytochemical Screening of Venpoosani Lekiyam – A Poly Herbal Siddha Formulation

Synthesis And Evaluation Of Herbal Formulation Using Natural Extract

Our study is intended to prepare and evaluate the polyherbal formulation (PHF) using Nyctanthes arbor-tristis, Piper nigrum, and lemon juice. Poly Herbal Formulation F1 and F2 were synthesised and subjected to qualitative, quantitative phytochemical analysis and antioxidant activity was measured using a standard protocol. Antimicrobial activity of PHFs was observed by the agar well diffusion method. The anti-inflammatory and anti-diabetic activities (In-vitro) were studied in F1 by using inhibition of albumin denaturation method. TLC and FTIR are done for the structural elucidation in F1. The F1 and F2 exhibited the presence of flavonoids, alkaloids, tannins, phenols, terpenoids, carbohydrates, saponins, Glycosides and proteins. The F1 and F2 show significant activity against the standard in the antimicrobial and antioxidant activity. The presence of flavonoids and alkaloids may contribute to the anti-microbial activity. The Nitric oxide radical scavenging activity and a metal chelating assay of the F1 possess the highest antioxidant activity of 73.1% and 69% respectively. TLC confirms the presence of flavonoids in F1. FTIR spectrum shows the functional group of amines, alcohols, nitrites, aromatic and aliphatic compounds. These FTIR bands denote stretching and vibrational bands responsible for the compounds like flavonoids, terpenoids, phenols and amino acids. This investigation shows the excellent result in antimicrobial, antioxidant and in-vitro anti-diabetic and anti-inflammatory activities. In future, the PHF of F1 can be used as potent therapeutical agents and can be further exploited for their other beneficial effect in the pharmacological studies.

Thaalisapaththiri Chooranam (Poly Herbal Formulation) in Siddha Medicine - A literature review

Thaalisapaththiri chooranam (T.C) is a poly herbal formulation and used to treat a wide variety of respiratory tract conditions such as kaasam, shayam, eilai and pitha diseases in Siddha Medicine. It has different ingredients which are having a wide range of therapeutic uses. Although the method of preparation of the T.C is mentioned in the eight text books, same variety of ingredients (eighteen) is mentioned in only two books. Therefore, the preliminary step was to develop the documentary evidences for the medicinal ingredients those are using to prepare the T.C. Data for the review on 18 ingredients from 13 families were collected from relevant text books and research sources from October to December 2019. The characteristics of the ingredients that were identified for the review were morphology; native; parts used; siddha properties such as taste, potency and efficacy; pharmacological actions and phytochemicals. Among these ingredients, all were identified as herbal materials and 2(11.1%) of the species were found in Umbelliferae, Myristicaceae, Piperaceae, Combretaceae and Compositae families. Based on the morphology 8(44.44%) and 6(33.33%) plants were trees and herbs respectively; 8 species (44%) were used as dry fruits. Among these ingredients, 11(39.2%) were pungent in taste, 15(83.3%) hot potency and 12(66%) pungent efficacy. Based on pharmacological actions such as carminative [12(27%)] and stimulant [11(23%)]; phytochemicals such as volatile oil 8(23%) were found in these ingredients. Although this review provides useful documentary evidences for T.C in health management further extensive scientific studies should be carried out to justify in future.

Formulation, Phytochemical Estimation of Active principles by Spectroscopic methods and Comparison of the Poly Herbal Antidiabetic Tablet with the Commercial Tablets

Diabetes mellitus is a group of metabolic disorders characterized by increased glucose levels over a prolonged period. The poly-herbal anti-diabetic tablet was formulated in the laboratory by using herbs of Enicostemma littorale (EL) (Gentianaceae), roots of Aconitum heterophyllum (AH) (Ranunculaceae), rhizomes of Picrorhiza kurroa (PK) (Scrophulariaceae), and fruits of Piper longum (PL) (Piperaceae). Furthermore, Laboratory formulation (LF) and Marketed formulations (MFs) were tested for Phytochemical screening and Qualitative chemical examination. From their result, isolation of active principles, Swertiamarin (EL-1) and Piperine (PN-1), was done and subjected to phytochemical examination by Modern analytical methods like TLC, UV, FTIR. Finally, the quantification and estimation of the aforementioned compounds were achieved by HPTLC. SSE of all the four formulations and the chemical tests proved alkaloids, flavonoids, carbohydrates, tannins, phenols and phytosterols. Comparing the data frommthe melting point, TLC, UV, and IR studies of EL-1 and PN-1 with standard Biomarkers, further calculation of both markers concentrations was done with the help of the calibration equation and the peak area derived from the chromatogram. Study outcomes signify that LF was more potent than MFs for anti-diabetic activity, signifying as a promising natural and safe remedy for the prevention of diabetic complications.

Wound Healing Efficacy of Re Cell Heal in a Diabetic Foot Infection Patient Diagnosed with Gas Gangrene – A Case Report

Gas gangrene is a rapidly progressive and bacterial infection that produces tissue gas in gangrene. The condition may result from ischemia, infection, or trauma or a combination of these processes. A 72-year-old woman with a 10 years history of type-2 diabetes mellitus (NIDDM) presented to the emergency department with a one-week history of left foot painful, swelling, redness, fever and foul-smelling discharge from wound. Wound infection of the left foot worsening progressing to gas gangrene that remains unhealed after a week to one month. She was diagnosed with Clostridium perfringens and Clostridial myonecrosis. The foot X-ray showed a 13 X 8.5 X 1.75 cm and is surrounded by callus under the 1st to 5th metatarsal heads (Figure 3a & 3b). Computed tomography (CT) scan showed moderate air suggesting deep tissue infection, with multiple tiny gas bubbles within under the first metatarsal heads, concerning for gas gangrene. The patient was treated with Poly Herbal Formulation (PHF), Re Cell Heal 500 mg Capsule (PRANATHI HERBALS; License No. T-2265/Ayur/; Letter No 2884/ DA/2018) (p.o) twice a day, in addition to the (Topical) Wound Check Ointment (Letter No 4964/DA/2018) accompanied with debriment over a five (5) months, foot healed without amputation improved therapeutic outcomes, reduce fatalities caused by gas gangrene. Re Cell Heal helps to provide relief in the symptoms like swelling and pain in tissues. Recent scientific evidences and trials conducted on Re Cell Heal is a traditional and alternative medicine in wound therapy, gas gangrene and diabetic related foot problems holds good promise in the future safety without any side effects.

Bioactive Constituent Profiling of Poly Herbal Distillate Thiribhaladi Dravagam to Evaluate Its Credible Therapeutic Role in Siddha Clinical Practices

Introduction: Hydro distillation in Siddha medicine is the technique of extracting valuable principles from a pre-processed raw drug through the traditional process of distillation under a controlled temperature setting. Thiribhaladi Dravagam (TD) is one of the polyherbal distillate formulation stated in the Tamil medical text specified for phlegmatic affections. The drug was subjected to GC-MS studies to screen its bioactive sketch. Methods: Two sets of raw drug samples from different locations were collected. The raw drugs were purified and pre-processed as per the classical literature and distilled in a traditional apparatus. Two distillate samples (TD 1 & TD 2) were prepared from the set of ingredients and were screened for its constituents. Results: The Gas chromatogram of TD 1 revealed 4 prominent peaks with retention time ranging from 4.84 to 8.27. TD 2 reported 8 prominent peaks with retention time ranging from 3.35 to 7.88. The study testified the presence of active volatile metabolites like aldehydes and oxygenated monoterpenes like citral and borneol, monocyclic alcoholic compounds like terpineol and organic acids like caprylic acid from both the samples. TD 2 reported more pharmacologically valuable lead molecules than TD 1, which may be due to the variation in diversity of the two sets of raw drug samples. Conclusion: The bio constituents of TD was found to be pharmacologically significant from the review of various reputed research works supporting the traditional claim on its role in the respiratory system and for maintaining good health. Through the review of Thiribhaladi Dravagam and its analytical part performed through GC-MS, the medical applications of TD in multiple clinical associations and as a perfect health promoter has been justified for promoting its traditional value.

Evaluation of the Antioxidant and Antidiabetic Potential of the Poly Herbal Formulation: Identification of Bioactive Factors.

The present investigation is intended to prepare a Poly Herbal Formulation (PHF) with Piper nigrum (fruits), Terminalia paniculata (bark) and Bauhinia purpurea (bark) and assess their antioxidant and glucose-lowering effects utilizing in vitro models. The individual plant methanolic extracts and PHF are exposed to phytochemical examination and to distinguish the bioactive factors by GC-MS. We assessed the antioxidant properties of individual plant extracts and the PHF by using the DPPH scavenging method, H2O2 scavenging assay, TBARS assay and total antioxidant estimation. Likewise, the anti-diabetic activity was assessed by ɑ-amylase and α-glucosidase enzyme inhibition and glucose diffusion inhibitory techniques. We found that PHF contains a high measure of total phenolics, total flavonoids and tannin compared to individual plant extracts. The GC-MS identified the bioactive components. We also found that PHF had significantly higher antioxidant and glucose-lowering effects than the individual plant concentrates. In conclusion, it could be reasoned that due to the nearness of antioxidant components, the PHF has good potential in the administration of hyperglycemia, diabetes and the related state of oxidative stress. This study shows that PHF is superior to individual plant extracts, supporting the conventional PHF concept.

Formulation and Evaluation of a Poly Herbal Anti-acne gel

Formulation and Evaluation of a Poly Herbal Anti-acne gel

Phytochemical Screening, Spectroscopic Examination and Antimicrobial Evaluation of PHE (Poly Herbal Extract) of Selected Indian Medicinal Herbs

Methanolic extract of important medicinal herbal plants like Marsilea-quadrifolia (whole plant), Trigonella-foenum-graecum (whole plant), Leaves of Mangifera-indica, Seeds of Eugenia-jambolana, Flowers of Cassia-auriculata, Flower of Musa- paradisiaca, Leaves of Aegle-marmelos and equal ratio of the mixture of the seven plants (Poly Herbal Extract - PHE) was prepared. The phytochemical screening for all the individual plants and PHE have been studied by using UV, IR, TLC and AAS. The results obtained from spectroscopic analysis indicated the presence of alkaloid, carbohydrates, protein, phenols, tannin, saponin, triterpenoids, glycosides, phytosterols, gums and mucilage. The results of TLC for seven plants and PHE showed the Rf factor in confirmation with phytochemical and spectrometric analysis. The atomic absorption studies reflected the presence of copper, iron, magnesium and zinc metals in PHE. Antimicrobial studies for all the individual plants and PHE were carried towards gram positive bacteria Bacillus subtilis and gram negative bacteria Pseudomonas aeruginosa, Escherichia coli respectively. The in vitro antimicrobial studies also revealed that PHE showed better activity against E. coli which is comparable with the standard ciprofloxacin.

Comparative Amino Acid Compositions of Uvaria chamae Stem Bark and Poly Herbal Mixture

The study evaluated comparative amino acids compositions of Uvaria chamae stem bark and Ruzu bitters. The amino acids compositions were determined using amino acid analyzer. The result of amino acid composition showed that eighteen amino acids were detected in both samples. Ten of the detected amino acids were essential amino acids and eight were non-essential. Glumatic acid had the highest concentration of 10.90 and 5.15 g/100g protein in Uvaria chamae stem bark and Ruzu bitters followed by aspartic acid with values of 8.40 and 3.44 g/100 g protein in Uvaria chamae stem bark and Ruzu bitters respectively. Leucine was the next amino acid in Uvaria chamae stem bark and Ruzu bitters followed by arginine. Uvaria chamae stem bark had the highest level of total amino acids of 72.66 g/100 g protein and Ruzu bitters had 32.17 g/100 g protein. For the EAA, it was 34.41 g/100 g for Uvaria chamae >17.44 g/100 g for Ruzu bitter. The highest essential amino acid (EAA) was leucine (6.13 and 3.56 g/100 g) in Uvaria chamae stem bark and Ruzu bitters. The total sulphur amino acid was generally low at 1.01-1.78 g/100 g but the % Cysteine in total sulphur amino acid (TSAA) was slightly high at 47.05% for Ruzu bitters but lower in Uvaria chamae stem bark (27.44%). The percentage coefficient variance (CV %) of the amino acid values were generally high with the exception of isoleucine, phenylalanine, lysine, methionine, leucine, cysteine and alanine with respective CV % values of 27.32, 31.97, 33.68, 37.50, 37.53, 38.81 and 39.05 while rest of CV % values ranged from 50.69-94.53 showing the gap of the amino acid values in the two samples to each other. The results of this study indicate that Uvaria chamae stem bark is richer in essential amino acid while % Cys/TSAA value is higher in Ruzu bitters.

Poly Herbal Hair Oil Preparation, Standardization, Treatment and Evaluation for Alopecia in Male Wistar Rats

Poly Herbal Hair Oil Preparation, Standardization, Treatment and Evaluation for Alopecia in Male Wistar Rats

Formulation and Evaluation of Poly Herbal Hair Oil

The concept of beauty and cosmetics is as ancient as mankind and civilization. So, they use various beauty products that have herbs to look charming and young. Herbal cosmetics are now-a-days widely used by the common people because of concept of fewer side effects and with a better safety and security profile. The present work was aimed to formulate herbal oil for general purpose (application in hairs) using various herbs. The formulated herbal oil was evaluated and various parameters such as viscosity, saponification value, pH etc. were determined and are reported in this project.

Alcoholic Extract of Poly Herbal Powder Mixture for Anti-Obesity effect on Wistar Rats

Alcoholic Extract of Poly Herbal Powder Mixture for Anti-Obesity effect on Wistar Rats

Acute and Sub-acute Toxicity Studies of a Patented Anti-anxiety Poly Herbal Formulation

Acute and Sub-acute Toxicity Studies of a Patented Anti-anxiety Poly Herbal Formulation

Assessment of Therapeutic Outcome and Medication Adherence in Diabetics Consumed Insulin, Oral Hypoglycemics and Poly Herbal Drugs

: Introduction: The overwhelming of consumption of antidiabetes poly herbal products (PHP) would influence the outcomes of hyperglycemia management. The underlying reasons were yet to be reveled; thought to be the herb-drug interactions and delayed adherence towards the conventional anti-diabetes drugs. We hypothesized that consumption of poly herbal antidiabetes products would influence the therapeutic outcome while on treatment with Insulin, oral hypoglycemic (OHG) drugs and/or both. We also hypothesized that trends towards poly herbal antidiabetes products would change the adherence towards conventional drug treatments and life style modifications. Methods: A total of 1251 patients with both type 1 and 2 diabetes mellitus of age 20-60 year, their fasting blood sugar (FBS) >110 mg/dl and /or random blood sugar (RBS) >150 mg/dl and urine sugar level >50 mg/dl were enrolled. The patients used herbal products, steroids, pregnant and lactating women and diabetic micro vascular disorders were excluded. The physical and vital parameters, symptoms of hyper/hypoglycemia were monitored. Diabetes Self-Assessment Questionnaire 16 (DSMQ-16); the scale used to assess their glucose management, physical activity, dietary control and physician contact was administered to each patient as a measure of adherence to the treatment. Results: The adherence level towards Insulin was found to be decreased while oral hypoglycemic drugs showed well. The consumption of PHP was most prevalent as well as adherence level. The markers of hyperglycemia were found to be altered in patient consumed PHP along with OHG drugs (p<0.05). The random blood sugar (RBS) levels were not found to be normalized (p<0.05) while fasting blood sugar (FBS) were found to be responded (p<0.05). Conclusion: The PHP had a significant influence on OGH drugs. Since the adherence towards Insulin was decreased and assumed OHG-herb interactions the hyperglycemia management was not carried out. We recommend noncompartment Pharmacokinetic as well as Pharmacodynamics studies along with therapeutic drug monitoring.Key words: Poly herbal, Oral hypoglycemic drugs, Insulin, Adherence.

Poly herbal formulation with anti-elastase and anti-oxidant properties for skin anti-aging

BackgroundSkin forms an important part of human innate immune system. Wrinkles, thinning and roughening of skin are some of the symptoms that affect the skin as it ages. Reactive oxygen species induced oxidative stress plays a major role in skin aging by modulating the elastase enzyme level in the skin. Extrinsic factors that affect skin aging such as UV radiation can also cause malignant melanoma. Here we selected four medicinal plant materials, namely, leaves of Nyctanthes arbor-tristis, unripe and ripe Aegle marmelos fruit pulp and the terminal meristem of Musa paradisiaca flower and investigated their anti-aging properties and cytotoxicity in vitro individually as well as in a poly herbal formulation containing the four plant extracts in different ratios.MethodsThe phytochemical contents of the plant extracts were investigated for radical scavenging activity and total reducing power. Based upon its anti-oxidant properties, a poly herbal formulation containing leaves of Nyctanthes arbor-tristis, unripe and ripe fruit pulp of Aegle marmelos, and the terminal meristem of Musa paradisiaca flower in the ratio 6:2:1:1 (Poly Herbal Formulation 1) and 1:1:1:1 (Poly Herbal Formulation 2), respectively were formulated.ResultIt has been observed that the Poly Herbal Formulation 1 was more potent than Poly Herbal Formulation 2 due to better anti-oxidant and anti-elastase activities in NIH3T3 fibroblast cells. In addition Poly Herbal formulation 1 also had better anti-cancer activity in human malignant melanoma cells.ConclusionBased on these results these beneficial plant extracts were identified for its potential application as an anti-aging agent in skin creams as well as an anti-proliferation compound against cancer cells.

Formulation and Evaluation of a Poly Herbal Skin Care Cream containing Neem and Tulsi

Neem and Tulsi called as holy tree and herb has been used since ancient times for the treatment of various skin diseases. In this study Neem leaves Azadirachta indica and Tulsi leaves Ocimum sanctum was incorporated with various ingredients and a poly herbal cream was prepared. Neem leaves and Tulsi leaves were collected from Tirupati Local Area. Both these plants were shade dried for 4 days and size reduced using mixer grinder converted into coarse powder and passed through sieve number 22. The coarse powder was stored for further studies. Both Neem and Tulsi leaf were defatted using pet ether 60–40 and then extracted using ethanol in a soxhlet apparatus. The extraction was carried out for 3 hrs taking 100 gms of plant material with 500 ml of ethanol. The extract was evaporated in a rotary evaporator the obtained ethanol extracts were preserved for further studies in a dessicator. The extracts were incorporated with various ingredients and excipients and a poly herbal cream was formulated. The cream was evaluated for various evaluation parameters which include pH, viscosity, spreadability, centrifugation test, spectrophotometric test, accelerated stability studies and microbial stability. The accelerated stability studies were carried out for 20 days in room and elevated temperature in a stability chamber. The cream was found to be stable after centrifugation there was no phase separation after centrifugation and stability study. The microbial stability was evaluated for bacterial growth (E. coli) and yeast. The results revealed that the microbial stability of the cream incorporated no microbial growth.

Clinical Efficacy of a Unani Poly Herbal Formulation on Abnormal Vaginal Discharge

Clinical Efficacy of a Unani Poly Herbal Formulation on Abnormal Vaginal Discharge