Acetic Acid Derivatives Research Articles

In Vitro-Propagation of Agave tequilana Weber cv. azul in a Temporary Immersion System

In Mexico, there is a need to produce large quantities of plantlets for the establishment and replanting of blue (cv. azul) agave production areas. Most of these plots are within the origin denomination area (DOT, Spanish acronym) of the distilled product of this plant, known as tequila. The objective of this study was to develop an in vitro-propagation protocol for Agave tequilana Weber cv. azul using segmented stems in both: solid and liquid media. A disinfection and in vitro technique were developed to obtain shoots, through plantlets collected in commercial plots, which attained 100% surface-disinfection and budding rate. At the multiplication stage, the effects of 6-Benzylaminopurine (BA) (0.0, 4.4 and 13.2 μM) and kinetin (0.0, 9.4, 18.8 and 37.6 μM) were evaluated on lateral-shoot production of segmented sagittal stems. These were cultivated on Murashige & Skoog (MS) medium, with the addition of 3.0% sucrose and 8 g L−1 agar. It was observed that BA and kinetin increased the number of shoots per explant, obtaining up to 18 and 26, respectively. Furthermore, it was found that just the sagittal segmentation of explants increased axillary budding. On the other hand, segmented-stem bases were grown in MS liquid medium with 3.0% sucrose, inside a RITA® system, programmed by a 5 min immersion step with a frequency of every 4 h. The effect of Indole−3-Acetic acid (IAA) (0.57, 2.9, 5.7 μM) was evaluated, while maintaining a concentration of BA (13.2 μM). It was observed that the greatest concentration of IAA led to the formation of more than 20 buds per explant. These results offer a new methodology to increase the efficiency of A. tequilana Weber cv. azul-in vitro multiplication by sagittal segmentation of stems and the addition of BA and/or IAA.

Germination and in vitro development of mature zygotic embryos and protein profile of seedlings of wild and cultivated Hevea brasiliensis.

The main factors governing Hevea brasiliensis germination and seedling establishment remains unclear. We examined the effect of growth regulators Indole 3-Acetic Acid (IAA) and 6-Benzylaminopurine (BAP), and their interactions on germination and the development of mature zygotic embryos (MZE) and protein profile of Hevea brasiliensis seedlings from wild and cultivated (clone PB 250) genotypes. Embryonic axes excised from seeds (wild and clone PB 250) were inoculated in Murashige and Skoog medium (control) and supplemented with IAA (3 µM) and BAP (6 µM) individually and their combination (3 µM IAA + 6 µM BAP). For both genotypes, the mature embryos displayed a high percentage of germination and establishment, and the seedlings were characterized by protein bands ranging from 7 to 30 kDa. Notably, the wild genotype showed proteins in the 14 kDa range, which may be associated with one of the major rubber elongation factors (REF). The wild and clone genotypes presented different behavior and strategies in relation to the protein profile in the presence of different growth regulators. Although the latex biosynthetic pathway and its mechanisms of regulation still remain largely unknown, our results aid in our understanding of the dynamics of proteins in different rubber tree clones in vitro.

Preparation and In vitro Characterization of Aceclofenac Nanosuspension (ACNS) for Enhancement of Percutaneous Absorption using Hydrogel Dosage Form

Aceclofenac (AC) is an orally active phenyl acetic acid derivative, non-steroidal anti-inflammatory drug with exceptional anti-inflammatory, analgesic and antipyretic properties. It has low aqueous solubility, leading to slow dissolution, low permeability and inadequate bioavailability. The aim of the current study was to prepare and characterize AC-NS-based gel to enhance the dissolution rate and then percutaneous permeability. NS.s were prepared using solvent/antisovent precipitation method at different drug to polymer ratios (1:1, 1:2, and 1:3) using different polymers such as poly vinyl pyrrolidone (PVP-K25), hydroxy propyl methyl cellulose (HPMC-E5) and poloxamer® (388) as stabilizers alone and in combinations of two polymers (1:2 and 1:4 Drug: polymer ratio). Fifteen formulas of AC-NS.s were prepared and characterized for production yield, loading efficiency, particle size, polydispersity index and physical stability. The best formulas of NS were then lyophilized to be characterized by FTIR, DSC, P-XRD and SEM. After that, the best prepared formula of AC-NS regarding the involved characterization methods was incorporated in gel dosage forms using carbopol®940. From this study, we conclude that the dissolution rate and permeability of AC were improved when the particle size was reduced to Nano-scale as compared with pure drug.

Isolation and Characterization of Bacillus Spp. for Plant Growth Promoting Properties

Abstract A group of free-living bacteria known as plant growth-promoting rhizobacteria (PGPR) inhabit the rhizosphere and aid root development. These rhizobacteria bacteria are vital to the growth of plants and can serve as bio-fertilizer and can enhance food security through green agricultural practices. They exhibit special features which make them potential candidates as bio-fertilizer. Isolation and characterization of rhizobacteria is the first step toward their utilization as bio-fertilizers. Ten rhizobacteria from two different rice farms were isolated and characterized for plant growth promoting properties. The isolated rhizobacteria were identified morphologically, microscopically, biochemically, and molecularly. Plant’s growth promoting properties of these rhizobacteria was also analyzed which includes; Indole 3-acetic acid production (IAA), phosphate solubilisation, hydrogen cyanide production (HCN), ammonia production (NH3), and zinc solubilisation. Out of the ten isolates, three were found to have the best plant growth enhancing properties and were therefore the best candidates as bio-fertilizers. 16SrRNA study and phylogenetic analysis was performed in order to unravel the specie of these three isolates and they were identified as Bacillus subtilis, Bacillus niacini, and Bacillus cereus with accession numbers OM184294, OM1842295 and OM184296 respectively. These isolates have the potential to be used as bio-fertilizer, which would significantly contribute to food security.

Effects of process factors on performances of liquid membrane-based transfer of indole-3-acetic acid

The paper has aimed at studying the transfer of indole 3-acetic acid (IAA) from a feed aqueous solution to a stripping aqueous solution of NaOH using a chloroform bulk liquid membrane and trioctylamine (TOA) as a ligand (L). Initial molar concentrations of IAA in the feed phase, cIAA,F0 (10–4–10–3 kmol/m3), of TOA in the membrane phase, cL,M0 (10–2 and 10–1 kmol/m3), and of NaOH in the stripping phase, cNaOH,S0 (10–2 and 1 kmol/m3), were selected as process factors. Their effects on the final values of IAA concentration in the feed phase (cIAA,Ff) and stripping solution (cIAA,Sf), extraction efficiency (EF), distribution coefficient (KD), and recovery efficiency (ER) were quantified using multiple regression equations. Regression coefficients were determined from experimental data, i.e., cIAA,Ff,ex = 0.02–1 × 10–4 kmol/m3, cIAA,Sf,ex = 0.22–2.58 × 10–3 kmol/m3, EF,ex = 90.0–97.9%, KD,ex = 9.0–46.6, and ER,ex = 66.5–94.2%. It was found that cIAA,F0 had the most significant positive effect on cIAA,Ff and cIAA,Sf, whereas cNaOH,S0 had a major positive effect on EF, KD, and ER. A deterministic model based on mass transfer of IAA was developed and its parameters, i.e., mass transfer coefficient of IAA-L complex in the liquid membrane (0.82–11.5 × 10–7 m/s) and extraction constant (1033.9–1779.7 m3/kmol), were regressed from experimental data. The effect of cL,M0 on both parameters was significant.

Synthesis, characterization and biological evaluation of some 2-arylbenzoxazole acetic acid derivatives as potential anticancer agents

A series of 2-arylbenzoxazole compounds possessing a cytotoxic activity as potential anticancer agents has been synthesised. Oxidative coupling of benzaldehyde with o -aminophenol utilizing lead tetraacetate approach has been used to realize the synthesis of compounds 1 - 11 . The cytotoxicity of 1 - 11 have been screened against breast cancer cell line MCF-7 and human colon cancer cell line HCT-116 utilizing doxorubicin as reference drug. Among these compounds, 2-(3-benzyloxyphenyl)benzoxazole-5-acetic acid 5 and 2-(4-methoxyphenyl)benzoxazol-5-acetic acid 10 , are found to be promising cytotoxic compounds against MCF-7 cell line. In addition, this study shows that the presence of acetic acid group at position 5 of benzoxazole nucleus enhances the activity. Moreover, it is noticed that the presence of oxygen atom directly linked to the phenyl substituent improves activity. The results offer a new benzoxazole based template to design and develop novel antineoplastic agents.

Biodiversity of Culturable Endophytic Actinobacteria Isolated from High Yield Camellia oleifera and Their Plant Growth Promotion Potential

Camellia oleifera Abel. is one of the world’s four famous woody oil trees and has drawn increasing attention because of its high commercial value. Endophytes are microorganisms inhabiting inside plant tissues, and their functions vary with the change of host status and environmental parameters. To deepen our understanding of the interactions between C. oleifera and their endophytic actinobacteria, the present study investigated the four endophytic actinobacterial composition-residing high-yield C. oleifera trees. A total of 156 endophytic actinobacterial isolates were obtained distributed in 17 genera. Among them, Streptomyces was the dominant endophytic actinobacteria, followed by Nocardia, Amycolatopsis, Microbiospora, Micromonospora and other rare actinobacteria genera. Soil characteristics including soil pH and organic matter were found to play crucial roles in shaping the endophytic actinobacterial community composition. Furthermore, all isolates were studied to determine their plant growth-promotion traits, 86.54% could produce Indole 3-Acetic Acid, 16.03% showed nitrogen-fixing, 21.15% showed phosphorus solubilizing, and 35.26% produced siderophore. Under the glasshouse condition, some isolates exhibited growth promotion effects on C. oleifera seedlings with significant increase in spring shoot length and ground diameter. Altogether, this study demonstrated that C. oleifera plants harbored a high diversity and novelty of culturable endophytic actinobacteria, which represent important potential as natural biofertilizers for the high production of C. oleifera.

Comparative Extraction of Aluminum Group Metals Using Acetic Acid Derivatives with Three Different-Sized Frameworks for Coordination

We prepared acetic acid derivatives using three different frameworks, calix[4]arene, alkenyltrimethylol, and trihydroxytriphenylmethane, which differ in the number and size of their coordination sites. We further investigated the extraction properties for aluminum group metal ions. All three extraction reagents exhibited increased extraction compared with the corresponding monomeric compounds, owing to structural effects. The extraction reaction and extraction equilibrium constants were determined using a slope analysis. Their extraction abilities, separation efficiencies, and potential coordination modes are discussed using the extraction equilibrium constants, half-pH values, and spectroscopic data. The calix[4]arene and trihydroxytriphenylmethane derivatives demonstrated allosteric co-extraction of indium ions (In3+) with an unexpected stoichiometry of 1:2.

In Praise of the Phytogenic Medicinal Plant Syzygium Aromaticum: A Review

Syzygium aromaticum commonly known as Clove is considered to be the most precious spice among others, which has been continued to be utilized for centuries for different biological and therapeutic purposes. It is a tropical tree which belongs to the Myrtaceae family and natively originated from Indonesia. However, in current times it is found in a few other places of the world including Brazil. Clove is viewed as perhaps the most extravagant source of phenolic mixes, for example, eugenol, eugenol acetic acid derivatives and so on. These mixtures have incredible potential for restorative, organic and other rural applications. In this review we have tried to figure out some of the important medicinal or therapeutic as well as phytobiotic and agricultural utilizations of the products prepared from Clove. We have tried to give an extra emphasis on the application of clove as a product of herbal contraception however for this purpose dose of the extract (either aqueous or ethanolic) of clove plays the vital role in this case. Although for establishing or making clove products commercially available as a safe product for herbal contraception or other medicinal impacts, more studies are required, and it could open a new era in the field of herbal medicine that would be much safer and feasible.

Anticancer activity of N-heteroaryl acetic acid salts against breast cancer; in silico and in vitro investigation.

The present research was performed to assess N-heteroaryl acetic acid salts' anticancer activity against the breast cancer cell in order to introduce new inhibitory agents for histone deacetylase. A molecular docking simulation was performed to design the rational novel compounds. Afterward, the best compounds were selected for synthesis. The cytotoxic effects and mechanism of action have been studied via (Methyl Thiazol-Tetrazolium) MTT assay. Flow cytometry and gene expression analyses were performed to introduce an effective acetic acid derivative as an anticancer agent. Molecular docking simulations demonstrated that all compounds have the best interaction with histone deacetylase. The fold changes of Bcl-2, Bak, Bim, Caspase-3, and Caspase-8 gene expressions were investigated and compared with reference gene using real-time PCR. The cytotoxic studies showed the best anticancer activity of 4-benzyl-1-(carboxymethyl) pyridinium bromide (compound 2) with a low IC50 value (32µM, p < 0.05). Also, the best anti HDAC activity was obtained for compound 2 with IC50 value of 1.1µM. Furthermore, this compound showed a high percentage of apoptosis among all tested compounds after 72h incubation which was associated with the significant increase in mRNA level of Bim, Bax, Bak, Caspase-3, and Caspase-8 and the considerable decrease in Bcl2 gene expression. These results suggest that compound 2 with the benzyl ring could be an effective anticancer compound for further investigation in breast cancer treatment.

α‐Glucosidase, α‐Amylase Inhibition, Kinetics and Docking Studies of Novel (2‐Chloro‐6‐(trifluoromethyl)benzyloxy)arylidene) Based Rhodanine and Rhodanine Acetic Acid Derivatives

AbstractIn our effort to identify potent fluorinated small molecules as antidiabetic compounds, a novel fluorinated series of 2‐chloro‐6‐(trifluoromethyl)benzyloxy arylidene derived Rhodanine and Rhodanine‐acetic acid derivatives were synthesized and screened for α‐glucosidase and α‐amylase inhibitory activity. Newly synthesized compounds were characterized by 1HNMR, 13C NMR, and LCMS spectral data. Among the tested compounds, (Z)‐5‐(4‐(2‐chloro‐6‐(trifluoromethyl)benzyloxy)benzylidene)‐2‐thioxothiazolidin‐4‐one(5 a) and 2‐((Z)‐5‐(4‐(2‐chloro‐6‐(trifluoromethyl)benzyloxy)benzylidene)‐4‐oxo‐2‐thioxothiazoli‐din‐3‐yl)acetic acid(6 a) emerged as most promising α‐glucosidase inhibitors with IC50 values 4.76±0.64 μM and 4.91±0.45 μM, respectively. Further, the kinetic inhibition experiments against yeast α‐glucosidase for compounds 5 a and 6 a indicated that these are competitive inhibitors with inhibitory constant (Ki) 0.54 μg and 1.15 μg respectively. Molecular docking studies on α‐glucosidase was performed by homology modelingfor the most potent compounds 5 a and 6 a to understand the putative binding mode. The study revealed substantial binding of the compounds to the active site of α‐glucosidase, indicating that the position of the substituent plays a key role in its inhibitory potential.

Development of an engineered methanotroph-based microbial platform for biocatalytic conversion of methane to phytohormone for sustainable agriculture

Methane is an important target for reducing greenhouse gases emission, owing to its abundance and highly global warming potential. Bioconversion of methane to value-added products, therefore, has become a promising and sustainable approach for industrial biomanufacturing. Herein, we metabolically engineered an obligate methanotroph Methylotuvimicrobium alcaliphilum 20Z to overproduce L-tryptophan and its derivative indole 3-acetic acid (IAA), a phytohormone, from methane. The engineered RS00 strain initially produced tryptophan from methane with a titer of 39.6 mg/L at 144 h of cultivation. The highest titer of IAA of 10.5 mg/L from methane was obtained in RS01 strain under Ptac promoter. Moreover, xylose, a carbon source derived from lignocellulosic biomass used as co-substrate, could enhance the titers of tryptophan and IAA in RS00 and RS01 strains by 60% and 82%, respectively. Remarkably, we proposed a proof-of-concept for the development of methanotroph-based plant growth-promoting bacteria (PGPB) which can utilize and convert methane into phytohormones to induce beneficial adaptation and growth promotion of plants. In this study, under saline-alkaline conditions, the germination percentage and elongation of the shoots and roots of wheat seeds treated with the IAA-producing strain were significantly improved, being 114%, 199% and 362% higher than those of control treatments, respectively. Our findings provide a novel methanotrophic platform for the conversion of renewable sources to value-added products. Additionally, the strategies and concepts presented here could be further expanded and applied to other methanotrophic hosts to develop biofertilizers that promote plant growth while mitigating methane and reducing chemical fertilizer use for sustainable agriculture production.

Lead tetraacetate assisted formation of bis(acetoxy)acetic acid derivative from carvone

R-Carvone on heating with Pb(OAc)4 in benzene gives (2RS)-2-hydroxy-2-[(1S)-4-methyl-5-oxocyclohex-3-en-1-yl)]-prop-1-yl bis(acetoxy)acetate. Alkaline hydrolysis of this compound affords the corresponding diol which under acidic catalysis is transformed into bis-hydroxy ether and 1,4-dioxane derivatives.

Are acetic acid derivatives really negative to the iodoform test?

Acetic acid derivatives such as ethyl acetate have been considered to be negative to the iodoform test because of the predominant hydrolysis leading to acetic acid. We clarified the immiscible property of the ester was the actual reason for the negative result. When THF or 1-propanol was used as a solvent, even alkyl acetate underwent the iodoform reaction; however, it cannot be used as qualitative test because of high solubility of iodoform into these solvents. This problem was overcome by conducting the test in methanol. Indeed, not only alkyl acetates but also N,N-dimethylacetamide showed positive to the iodoform test producing a yellow precipitates.

Effects of Epichloësinensis Endophyte and Host Ecotype on Physiology of Festuca sinensis under Different Soil Moisture Conditions.

This study explored the effects of the Epichloë sinensis endophyte on growth, photosynthesis, ionic content (K+ and Ca2+), phytohormones (abscisic acid—ABA, cytokinin—CTK, indolE−3-acetic acid—IAA, and gibberellin—GA), and elements—C, N, P (in the shoot and root) in two ecotypes of Festuca sinensis (ecotypes 111 and 141) under different soil water conditions (35% and 65% relative saturation moisture content (RSMC)). The results showed that 35% RSMC inhibited the plants’ growth, and compared with 65% RSMC, there was a significant (p < 0.05) decrease in the growth and photosynthesis indices, the contents of CTK and GA, Ca2+ concentration, and the contents of C, N, and P (in both the aboveground and underground parts) under 35% RSMC. E. sinensis had beneficial effects on host growth and stress tolerance. Under both 35% and 65% RSMC, the presence of E. sinensis significantly (p < 0.05) increased host plant height, tiller number, root length, root volume, shoot dry weight, chlorophyll content, and the rate of photosynthesis of both ecotypes. Furthermore, the shoot C, N, and P contents in plants infected with E. sinensis (E+) from the two ecotypes, under both conditions of RSMC, were significantly (p < 0.05) higher than those in corresponding plants that were not infected with E. sinensis (E−). Under 35% RSMC, the contents of ABA, K+, Ca2+, and root P contents in E+ plants were significantly (p < 0.05) higher than those in corresponding E− plants in both ecotypes. However, under 65% RSMC, root C, N, and P contents in E+ plants of ecotype 111 and 141 were significantly (p < 0.05) higher than those in corresponding E− plants. In addition, the host ecotype also had effects on host growth and stress tolerance; the growth and photosynthetic indices of ecotype 141 were significantly (p < 0.05) higher than those of ecotype 111 under 35% RSMC, which suggested that ecotype 141 is more competitive than ecotype 111 under water deficiency conditions. These findings suggest that the endophyte improved the host plant resistance to water deficiency by maintaining the growth of the plant, improving photosynthesis, accumulating K+ and Ca2+, promoting nutrient absorption, and adjusting the metabolism of plant hormones.

Utilization of a complex arrangement approach for spectroscopic examination with Eosin Y of various cephalosporins in their pure or pharmaceutical dosage forms, and in human plasma.

Approved, basic, effective and successful spectroscopic strategies (spectrophotometric and spectrofluorimetric) were created to measure seven cephalosporins: cefpiramide (I), cefuroxime (II), cefoxitin (III), ceftazidime (IV), cefpirome (V), ceftobiprole (VI), and ceftriaxone (VII). These strategies used a two-fold complex arrangement response for the drug amino groups with Eosin Y (EY). The examined drugs were determined spectrophotometrically at 542-550 nm in acetic acid derivative buffer. The examined drugs were determined spectrofluorimetrically by measuring their quenching effect on EY local fluorescence at 545 nm after excitation at 305 nm. The absorbance-intensity plots were rectilinear over the ranges 20-100, 10-130, 20-220, 30-230, 10-210, 20-180 and 10-130 μg ml-1 for I, II, III, IV, V, VI, and VII samples, respectively. The fluorescence-intensity plots were rectilinear over the ranges 0.5-1.5, 0.1-0.9, 0.3-1.5, 0.5-2.5, 0.1-0.9, 0.5-2.5 and 0.1-1.0 μg ml-1 for I, II, III, IV, V, VI, and VII samples, respectively. The recommended materials were certified as adhering to International Council for Harmonisation (ICH) guidelines and were used to examine the tested drugs in different dosage forms and in human plasma tests. The approved materials matched the reference materials.

New Derivatives of (Carbamoselenoyl)acetic Acid for the Synthesis of Functionally Substituted Selenazoles

New Derivatives of (Carbamoselenoyl)acetic Acid for the Synthesis of Functionally Substituted Selenazoles

Rapid mass propagation of endangered valuable medicinal plant &lt;em&gt;Salacia chinensis&lt;/em&gt; L. and GC-MS/LC-MS analysis of active compounds produced in callus and leaf extracts

A protocol for indirect organogenesis of Salacia chinensis was established. Normally S. chinensis is propagated through seeds. However, due to the difficulty to obtain uniform plants in a short time period by seed germination, micro-propagation is a possible alternative method. For the micro-propagation, media with different concentration combinations of cytokinins and auxins were used to induce callus formation in three explants types: leaf segment, nodal segment and seeds. The rate of recurrence of callus formation from leaf on Murashige and Skoog (MS) basal medium supplemented with thidiazuron (TDZ) 0.5mg and TDZ 1mg was 100% and the maximum percentage of shoot let development from nodal segment on MS basal medium supplemented with BAP 6-(benzylamino purine) 3.5+ indole3-butyric acid (IBA) 1 mg/l was 78.3% when compared to other plant growth regulators (PGR) combinations used. The highest shoot regeneration response (85%) and the determined shoots (12.33 ± 0.33%) per callus were attained from leaf explants on MS medium containing 1-napthaleneacetic acid (NAA) 1mg/l + BAP 0.5 mg/l. The seeds showed highest percentage of shoot formation on MS medium supplemented with BAP 2mg + indole 3-acetic acid (IAA) 2mg/l and BAP 2mg + isopentenyl adenine (2iP) 2mg/l. Highest root formation (70±1.3%) was found in shoot regenerated using leaf segment on MS medium supplemented with IBA 0.5mg. The gas chromatography mass spectrometry (GC-MS) analysis of methanolic extract of callus showed more compounds at higher percentage. HPLC-MS analysis of methanolic extract of callus showed higher concentration of Mangifera than in leaf extracts are reported for the first time.

Statistical optimization of in vitro callus induction of wild and cultivated varieties of Mucuna pruriens L. (DC.) using response surface methodology and assessment of L-Dopa biosynthesis

Mucuna pruriens L. (DC.), is a valuable, economically significant medicinal crop, known worldwide for producing the anti-Parkinson's drug Levodopa (L-Dopa). Itching property of the plant poses certain constrains in its conventional breeding, but, in vitro cell culture systems offer advantage in which controlled conditions can be used for the production of valuable compounds. In the current investigation, a simple and efficient method for callus induction from the wild (var. pruriens) and cultivated (var. utilis) varieties of M. pruriens is described. Juvenile leaves and roots obtained from 30 day old aseptically grown seedlings were used as explants for callus induction. The leaves were found to be the best explants for obtaining maximum callus within a short period of time in 2.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) supplemented Murashige and Skoog (MS) medium. White compact root callus was produced from var. utilis in 2,4-D (2.0 mg/L) and indole 3-acetic acid (0.2 mg/L) + benzyl amino purine (2.0 mg/L) containing media. Whereas, callus formation was not observed from root explants of var. pruriens. Central composite design–response surface methodology (CCD-RSM) experiment was designed to analyze the linear-model and quadratic model (including pair-wise and interactive effects) of each variable to study the maximum biomass production from leaf and root explants. In this study, 1.5 mg/L concentrations of BAP as well as 2,4- D in plant tissue culture medium resulted in maximum callus biomass (2.378) and L-Dopa % yield (0.635 %). L-Dopa content in these varieties was investigated by high performance-thin layer chromatography (HP-TLC) using the methanolic extract prepared from callus cultures of both the varieties and compared with natural plant parts. L-Dopa content was found to be higher in seeds obtained from the naturally grown plants of var. pruriens followed by var. utilis (> 0.55 % and 0.494 % respectively). From the result obtained it can also be ascertained that root callus can serve as a source of L-Dopa extraction commercially.

Auxin treatment increases lifespan in Caenorhabditis elegans.

ABSTRACTThe auxin-inducible degradation system (AID) has proven to be a highly versatile technology for rapid, robust and reversible depletion of proteins in multiple model systems. In recent years, AID has been adapted into the nematode Caenorhabditis elegans as a tool for conditional protein knockdown. Numerous transgenic strains have been created that, upon auxin exposure, undergo protein inactivation in the worm germline or somatic tissues, both during development and in young adults. Since longevity assays often involve long-term gene- and protein-manipulation, the facility for spatiotemporally precise and extended protein removal makes AID a potentially highly valuable tool for aging biology. However, whether auxins themselves impact worm longevity has not been directly addressed. Here, we show that prolonged exposure to indole 3-acetic acid (IAA), the auxin used in worm AID studies, extends lifespan. We also report that two transgenic strains expressing Arabidopsis proteins that are key components of the AID platform are longer lived than wild-type animals. Together, our results highlight the necessity for exercising caution while utilizing AID for longevity studies and in interpreting the resulting data.This article has an associated First Person interview with the first author of the paper.