Moringa Oleifera Aqueous Extract Research Articles

13-Week repeated-dose toxicity study of optimized aqueous extract of Moringa oleifera leaves in mice

Ethnopharmacological relevanceMoringa oleifera (Moringaceae family), commonly known as horseradish or tree of life, is traditionally used for various diseases, such as diabetes, hypercholesterolemia, neurological disorders, among others. Aim of the studyTo evaluate the toxicological profile of the oral use of an aqueous extract of Moringa oleifera leaves for 13 weeks in mice. Materials and methodsInitially, a factorial design (23) was carried out to optimize aqueous extraction using as variables; the extraction method and proportion of drug. The 13-week repeated-dose toxicity trial used female and male mice, with oral administration of aqueous extract of Moringa oleifera leaves at doses of 250, 500, and 1000 mg/kg. The animals were evaluated for body weight, water and feed intake, biochemical and hematological parameters, urinalysis, ophthalmology and histopathology of the liver, spleen and kidneys. ResultsThe extraction efficiency was evidenced by the extraction by maceration at 5%, obtaining the optimized extract of Moringa oleifera (OEMo). The oral administration of OEMo did not promote significant difference (p > 0.05) in the weight gain, food and water consumption of the control animals and those treated with 250 and 500 mg/kg. However, treatment with 1000 mg/kg promoted a reduction (p < 0.05) in food intake and body weight from the 7th week onwards in male and female mice. No alterations were detected in the hematological and histological parameters in the concentrations tested for both sexes. The highest concentration treatment (1000 mg/kg) promoted an increase in transaminases in males and females. All concentrations promoted a significant decrease (p < 0.05) in the serum lipid profile of mice. ConclusionThis study developed an optimized extract of Moringa oleifera leaves, which should be used with caution in preparations above 500 mg/kg for the long term because it leads to significant changes in liver enzymes. On the other hand, the extract proved to be a promising plant preparation for hyperlipidemia in mice.

Moringa oleifera leaf extracts improve exercise performance in young male adults: A pilot study

BackgroundMoringa oleifera leaves are rich in bioactive substances. PurposeThe purpose of this study was to evaluate the effects of Moringa oleifera leaf aqueous extract supplements on energy metabolism and antioxidant function in young male adults. MethodsForty-four young male adults (26.3 ± 3.5 years) were randomly assigned to two groups: a supplement group (n = 23) receiving aqueous extract of Moringa oleifera leaves and a placebo group (n = 21). The supplementation period lasted for 30 days. Baseline measurements were taken at the beginning of the study, and further measurements were taken at the end of the supplementation period. Changes in upper- and lower-body strength, treadmill endurance, and certain blood biochemical parameters were evaluated. ResultsAfter 30 days of supplementation, participants in the supplement group exhibited enhanced performance in push-ups and treadmill exhaustion tests compared to the placebo group. Levels of glucose, urea, malondialdehyde, and glutathione peroxidase activity in serum were also improved in the supplement group. ConclusionThe findings suggest that Moringa oleifera leaf aqueous extracts have the potential to improve post-exercise energy metabolism and antioxidant function in young male adults.

Interaction between aqueous extract of Moringa oleifera leaves and rabeprazole in treatment of ethanol induced gastric ulcer in Wistar rats

Background: The effect of concomitant use of herbs and conventional drugs is not widely known because it hasn’t been a common practice especially among the elite and those resident at the urban centers but very common among the rural duelers for co-administered herbs/supplements and conventional drugs. This study was carried out to investigate the interaction between moringa oleifera and rabeprazole in gastric acid secretion. Methods: Twenty (20) adult Wistar albino rats of both sexes weighing 300-450 g were randomly allocated into four (4) groups of five (5) animals per group. Group 1 served as the control and was given normal saline without administration of acid alcohol. Animals in group 2 were given acid alcohol (0.25 ml 0f 36% HCl + 75% ethanol 0.25 ml/100 g body weight). Animals in group 3 were given 50 mg/100 g bw of Moringa oleifera 1 hour before acid alcohol was administered. Group 4 were given: Rabeprazole 20 mg /kg body weight + acid Alcohol (36% HCL 0.25 mls+ 75% Ethanol (0.25 mls). A 30% isoflorane (inhalational anesthesia) by API Manufacturer with FDA, UK, marketed by Macfes medical store, high level Markurdi, Benue State, Nigeria. And 3.5% isoflurane (of the mixture of isoflurane 30%) at 100% oxygen was soaked in a cotton wool and dropped in clean and covered plastic container, and was used to anesthetized the rats, for the purpose of the aqueous extracts administration through a gastric fistula created by a surgical procedure. 10 minutes after aqueous extracts administration, aliquot samples were collected over 4 hours. Gastric acid secretion was measured by titrating the aliquots to a phenolphthalein endpoint. Results: showed that Moringa Olefera significantly delayed the time of onset of action of rabeprazole when co-administered. Moringa alone was a better inhibitor of gastric acid secretion than moringa co-administered with rabeprazole. Conclusion: Moringa delays the time of onset of action of Rabeprazole when co-administered.

Phytochemical Profiling by UHPLC-Q-TOF/MS and Chemopreventive Effect of Aqueous Extract of Moringa oleifera Leaves and Benzyl Isothiocyanate on Murine Mammary Carcinogenesis.

Moringa oleifera Lam, commonly known as moringa, is a plant widely used both as a human food and for medicinal purposes around the world. This research aimed to evaluate the efficacy of the aqueous extract of Moringa oleifera leaves (MoAE) and benzyl isothiocyanate (BIT) in rats with induced breast cancer. Cancer was induced with 7,12-dimethylbenz[a]anthracene (DMBA) at a dose of 60 mg/kg by orogastric gavage once only. Forty-eight rats were randomly assigned to eight groups, each consisting of six individuals. The control group (healthy) was called Group I. Group II received DMBA plus saline. In addition to DMBA, Groups III, IV, and V received MoAE at 100, 250, and 500 mg/kg/day, respectively, while Groups VI, VII, and VIII received BIT at 5, 10, and 20 mg/kg/day, respectively. Treatment was carried out for 13 weeks. Secondary metabolite analysis results identified predominantly quercetin, caffeoylquinic acid, neochlorogenic acid, vitexin, and kaempferol, as well as tropone, betaine, loliolide, and vitexin. The administration of MoAE at a dose of 500 mg/kg and BIT at 20 mg/kg exhibited a notable decrease in both the total tumor count and the cumulative tumor weight, along with a delay in their onset. Furthermore, they improved the histological grade. A significant decrease in serum levels of VEGF and IL-1β levels was observed (p < 0.001) with a better effect demonstrated with MoAE at 500 mg/kg and BIT at 20 mg/kg. In conclusion, this study suggests that both the aqueous extract of Moringa oleifera leaves and the benzyl isothiocyanate possess antitumor properties against mammary carcinogenesis, and this effect could be due, at least in part, to the flavonoids and isothiocyanates present in the extract.

Investigating the Anticancer Properties of the Essential Oil and Aqueous Extract of Moringa oleifera and its Biosynthesized Metal Nanoparticles on MCF-7 and BT-549 Cell Lines

Investigating the Anticancer Properties of the Essential Oil and Aqueous Extract of Moringa oleifera and its Biosynthesized Metal Nanoparticles on MCF-7 and BT-549 Cell Lines

Antibacterial effect of Moringa oleifera on Staphylococcus aureus and Pseudomonas aeruginosa isolated from raw milk and some dairy products with special reference to biofilm gene expression.

Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) are well defined as food poisoning pathogens that are highly resistant and need continuous studies. The purpose of the work was to examine phenotypic and genotypic characteristics of both P. aeruginosa and S. aureus, and treatment trials with medicinal plants. Samples were examined for isolation of P. aeruginosa and S. aureus on selective media followed by biochemical confirmation, biofilm formation, genes detection, and expression of P. aeruginosa pslA biofilm gene was performed by quantitative real-time polymerase chain reaction after treatment with 0.312 mg/ml Moringa oleifera aqueous extract as a minimum inhibitory concentration. The highest isolation rate of P. aeruginosa was 20% from both raw milk and Kariesh cheese, followed by 16% and 12% from ice cream and processed cheese, respectively, while the highest isolation rate of S. aureus was 36% from raw milk followed by 28% in ice cream and 16% in both Kariesh cheese and processed cheese. 30% of P. aeruginosa isolates were biofilm producers, while only 21% of S. aureus isolates were able to produce biofilm. The P. aeruginosa isolates harbor virulence-associated genes nan1, exoS, toxA, and pslA at 100%, 80%, 40%, and 40%, respectively. Staphylococcus aureus SEs genes were examined in S. aureus strains, where SEA and SEB genes were detected with 60%, but no isolate harbored SEC, SED, or SEE. The significant fold change of P. aeruginosa pslA expression was 0.40332 after treatment with M. oleifera aqueous extract. Pseudomonas aeruginosa and S. aureus harbor dangerous virulence genes that cause food poisoning, but M. oleifera extract could minimize their action.

Histological Study Of Protective Effects Of The Aqueous Extract Of Moringa Oleifera Seeds On Diclofenac-Induced Livers In Male Rats

The study designed to indicate the protective effect of the hot aqueous extract of Moringa oleifera the seeds of against the harmful effects stimulated by the diclofenac drug in the livers of the male rats. This study was carried out in the animal house of Science College / Department of Biology -University of Kufa from 1/10/2022 to 10/1/2023.&#x0D; Thirty animals of adult male rats of type (Rattus rattus) were used in this study which had an average weight of (220 g) and an average age of (12) weeks. The experimental animals of male rats were divided into six groups and each group included five male rats which were treated by dose Orally by means of gastric dosing tool for 35 days andwere as : the 1 group was provided with diet and water only and was a control group, while the 2 group of male rats was dosed with only diclofenac drug at a concentration of (100) mg / kg of body weight, and the 3 group of animals were dosed with hot aqueous extract of plant seeds Moringa oleifera at concentrationof (400 ) mg / kg of body weight and the 4 group of animals were dosed with hot aqueous extract of plant seeds Moringa oleifera at concentrationof (600 ) mg / kg of body weight respectively, while 5 group was dosed with the hot aqueous extract of the seeds of Moringa oleifera at concentration (400 mg/kg of body weight + diclofenac at a concentration of (100) mg/kg of body weight and the last group 6 was dosed with the hot aqueous extract of the seeds of Moringa oleifera at concentration (400 mg/kg of body weight + diclofenac at a concentration of (100) mg/kg of body weight respectively.all the animals of rats were treated in all groups for a period of 35 days and after expermnit end the rats were sacrificed after being anesthetized with chloroform and the livers was extracted from the bodies after they were opened and placed in formalin solution at a concentration of 10% for a period of 48 hours for the purpose for histological study.&#x0D; &#x0D; &#x0D; The results of this study showed that histological sections of liver tissue of rats treated with the aqueous extract of Moringa oleifera seeds at a concentration of (450 and 600) mg/kg of body weight and there were no pathological changes in the histological structure of the liver as the central vein, sinusoids, and hepatocytes were normal , as the results also revealed by examining the histological sections in the group treated with aqueous extract of Moringa oleifera seeds at concentrations of (450,600) mg/kg of body weight + diclofenac at a concentration (100) mg / kg of body weight the liver tissue was closer to the normal structure with slight changes observed in it such as little necrosis in some liver cells with little widening in sinusoids compared to the control group while the results of the current study showed that the liver tissue of rats treated with diclofenac at a concentration of (100) mg / kg of body weight had suffered from many pathological changes such as expansion of the central vein, widening of the sinusoids, necrosis and degeneration of the liver cells and necrosis of the liver tissue, and infiltration of inflammatory cells in comparison with the normal histological structure of the livers in the control group.&#x0D; &#x0D; &#x0D; &#x0D; &#x0D; &#x0D; &#x0D; &#x0D; &#x0D; &#x0D; &#x0D; &#x0D; &#x0D; &#x0D; &#x0D; &#x0D;

Effect of Aqueous extract of Moringa Olivera leaves( AEMOL) on some characteristics of ram epididymal sperms under cooling conditions

The current study was conducted in the Graduate Studies Laboratory at the College of Agriculture, Al-Muthanna University, for the period from December 3, 2022 to March 9, 2023, to determine the effect of an aqueous extract of Moringa leaves on some characteristics of epididymal sperm of rams under cooling conditions for different periods. The aqueous extract of Moringa leaves was prepared and stored in the refrigerator ( 5 °C) until the time of use, the testicles were transferred to the laboratory less than an hour after the slaughter of the animal, the caudal epididymis was diluted with a Tris extender containing 0.03 concentration of aqueous extract of moringa olivera leaves.The results of this study showed a significant increase (P&lt;0.01) in the percentage of live sperm, individual motility, and the plasma membrane integrity, while the adding of AEMOL leads to a decreasing of the percentage of abnormal sperms. Increasing the cooling hours of sperm leads to a significant decrease in the percentage of live sperm, individual motility, testing the integrity of the plasma membrane with an increase in the percentage of abnormal sperm. Using the aqueous extract of Moringa leaves at different times led to a significant effect on the individual motility, percentage of the plasma membrane integrity represented by the hypo-osmotic swelling test (HOST) , While the treatment did not significantly affect the percentage of live sperm and abnormal sperm. According to the results of this study we conclude that the aqueous extract of Moringa Oleifera leaves improves the characteristics of rams epididymal sperms preserved under cooling conditions for different periods

Effect of Adding Aqueous Extract of Moringa oleifera Leaves in Drinking Water on some of the Reproductive Characteristics for Lohmann Brown Rooster Breeder

The purpose of this study, which took place from December 8, 2022, to February 15, 2023, over the course of 10 weeks in the Department of Animal Production at Al-Qasim Green University’s Faculty of Agriculture, was to determine the impact, if any, that varying concentrations of an aqueous extract of the leaves of the Moringa Oleifera plant had on the reproductive and physiological qualities and the rates of hatching and fertilization for rooster breeders of the laying hen the first, a group that drank water without any flavorings or other additions, For the second, add 20 ml of an aqueous extract of Moringa oleifera leaves per litre of drinking water; for the third, add 40 ml; for the fourth, add 60 ml; for the fifth, add 80 ml; and for the sixth and final treatment, add 100 ml of an aqueous extract of Moringa oleifera leaves per litre of drinking water. As for estimating the individual movement of the sperm, we note from the results a superiority (P0.05) for the third, fourth, and fifth treatments compared to the first treatment in the fourth week. The results also showed a superiority (P0.05) for the second treatment in estimating the size of the ejaculate size compared to the first, third, and fifth treatments. The results revealed that the concentration of sperm increased significantly (P 0.05) after the second, third, fourth, and fifth treatments compared to the first.

The Influence of Feeding a Moringa Oleifera Leaf Aqueous Extract on the Biochemical Attributes of the Lohmann Brown Rooster Breed

This experiment was conducted at the Department of Animal Production in the Faculty of Agriculture at Al-Qasim Green University from December 8, 2022, to February 15, 2023. It lasted for 10 weeks and aimed to see what effect, if any, different concentrations of an aqueous extract of the leaves of the Moringa Oleifera plant had on the reproductive and physiological qualities and the hatching and fertilisation rates of rooster breeders of the laying hen. An aqueous extract of Moringa oleifera leaves can be added to 20 ml of drinking water to improve its nutritional value, while 40 ml of extract can be used to improve the nutritional value of 1 litre of water. A 60 ml dose of Moringa Oleifera leaf aqueous extract per litre of drinking water is recommended for the fourth treatment, while an 80 ml dose is recommended for the fifth. There were no statistically significant differences between the treatments for the follicle-stimulating hormone (FSH), and the findings revealed an increase at the level (P0.05) in the LH hormone in the fifth treatment compared to the control treatment (T1) and the other addition treatments. A comparison of the testosterone levels between Treatment 1 (control) and the other supplementary Treatments indicated that Treatment 4 produced significantly higher levels of testosterone (P0.01). Malondialdehyde (MDA) enzyme levels were found to be significantly lower in the fourth treatment compared to the control treatmenT1 and the rest of the supplementary treatments, with the third and fifth treatments also showing significant superiority over the control treatmenT1 and the second treatment (P0.05 for both comparisons).

Effect of Aqueous Extract of Moringa oleifera and Rosmarinus officinals on the Rate of Destruction of the Varroa parasite, Varroa destructor and Honey bees in the Field

Effect of Aqueous Extract of Moringa oleifera and Rosmarinus officinals on the Rate of Destruction of the Varroa parasite, Varroa destructor and Honey bees in the Field

Changes of Blood Biochemical Parameters During Immune Deficiency Prevention with an Extract of Moringa oleifera in Wistar Rats

The aim of this work was to determine the evolution of some biochemical parameters during a preventive immunostimulation assay of the total aqueous extract of Moringa oleifera leaves in rats. To do this, 48 albino rats including 24 males and 24 females divided into 12 groups of weight between 107 g and 140 g were used. The different doses of the aqueous extract of Moringa oleifera leaves 200 mg/kg bw, 400 mg/kg bw, 800 mg/kg bw and 1600 mg/kg bw respectively were compared with controls subjected to distilled water and 50 mg/kg bw of levamisole for 14 days. At the end of this 14-day treatment, immunosuppression was performed by administration of 5 mg/kg bw intraperitoneally dexamethasone in all rats grouped for three days twice daily. Then a two-week observation was made to assess the behavior of some biochemical parameters after administration of dexamethasone. During the experiment five samples were taken on day 1, day 14, day 17, day 21 and day 28. These different samples allowed the determination of biochemical and nutritional parameters. These samples were used to determine urea, creatinine, transaminases (AST/ALT), PAL, LDH, total cholesterol, HDL, LDL, RRC, CA, RC, IAP, triglycerides and finally nutritional parameters (albumin and total proteins).

Effect of ethanol and aqueous extract of Moringa oleifera on bacteria isolated from wound infection

The aqueous and ethanol extracts of Moringa oleifera used for the treatment of infectious disease were tested for their active against gram positive and gram-negative bacteria isolated from burns infection culture using the broth dilution and disc diffusion method. Results of this study revealed the presence of phytochemical which were active against gram positive and negative bacteria. Ethanol extracts of plant showed highest activity other than aqueous extract. The minimum inhibitory concentration (MIC) of the aqueous extracts on the test organism was 25- 100 mg\ml, while that of the ethanol extract ranged between 25 -50 mg\ml on the test organisms, the minimum bacterial concentration (MBC) ranging between 25-100 mg\ml for ethanol extract, and 25-200 mg\ml for aqueous extracts. The highest activity at 45 oC was demonstrated by the ethanol extracts of S. rosmarinus against Staphylococcus aureus and Klebsiella spp. In this study plant extracts against gram negative bacteria showed activity in acidic pH only in contrast of gram positive bacteria which were constant in plant extract. M. oleifera contained essential elements at higher levels. The results of this study suggest the possibility of using the ethanolic extracts of plant in treating diseases caused by the test organisms, especially when prepared at acidic pH.

Leukocyte Parameters and Immune Proteins Evolution during Prevention of Immune Insufficiency with an Extract of Moringa oleifera in Wistar Rats

Immunosuppression is a particular pathology reflecting a weakening of immune system and constituting a factor of exposure to other diseases. It is therefore both a disease and a risk factor. The aim of this work was to prevent a possible immune deficiency by using Moringa oleifera leaf extract. Forty eigth albino rats including 24 males and 24 females divided into 12 group weighing between 107g and 140g were used. Different doses of the leaves aqueous extract of Moringa oleifera respectively 200 mg/kg bw, 400 mg/kg bw, 800 mg/kg bw and 1600 mg/kg bw were compared with controls subjected to distilled water and 50 mg/kg bw of Levamisole for 14 days. At the end of this 14-day treatment, immunosuppression was achieved by intraperitoneal administration of 5 mg/kg bw of dexamethasone in all rats divided into groups for three days twice a day. Then a two-week observation was made to assess changes of the different immune cells after administration of dexamethasone. During the experiment, five samples were taken on day 1, day 14, day 17, day 21 and day 28. These different samples allowed the determination of hematological and immune proteins parameters. The obtained results showed an improvement in the immune status on day 14 with a highly significant increase (p&lt;0,001) in white blood cells, a non-significant increase in neutrophils, lymphocytes, monocytes and immature granulocytes unlike the control subjected to distilled water. The administration of dexamethasone on day 17, total leukocytes in rats of group V and VI decreased in significantly. Contrarily controls and doses of 200 and 400 mg/kg bw. From day 21 to day 28, the levels of the various immune cells increased significantly. In conclusion, 800 mg/kg bw and 1600 mg/kg bw of aqueous extract have a good profile to prevent possible immune deficiency.

Comparison fabrication, identification and avoidance of corrosion potential of M-CuO NPs/S-CuO NPs to suppress corrosion on steel in an acidic solution

The utilization of plants to produce metal oxide nanoparticles has recently received a lot of attention due to its ease of usage and environmental friendliness. Therefore, the aim of this study was to synthesize CuO NPs using two distinct methods, including an aqueous extract of moringa oleifera leaves (M-CuO NPs) and a synthetic approach (S-CuO NPs). Nanoparticles produced were evaluated by SEM, EDX, TEM and XRD, to consider the creation of CuO NPs and to determine the morphological, elemental constitution and size the samples. The average particles size 14.95 and 35.73 nm for S-CuO and M-CuO NPs, respectively, is having potentials for application as inhibitor in corrosion of MS. The inhibition and adsorption properties of prepared M-CuO NPs and S-CuO NPs on MS in 1 M HCl were investigated using the wt reduction assessment. The maximal IE of prepared M-CuO NPs and S-CuO NPs was 95.06% and 92.10%, respectively, at 1000 ppm. The IE % improves with greater concentration of the prepared M-CuO NPs and S-CuO NPs. According to the findings, M-CuO NPs are the most effective green potential inhibitor for MS in acidic conditions. It is demonstrated that the Langmuir isotherms are obeyed by the produced CuO NPs and MS substrate.

Characterization of Aqueous Extract of Moringa oleifera Leaves using GC-MS Analysis

Background: The Evaluation of plants for its bioactive compounds is of great importance to researchers because of their therapeutic properties which can be harnessed to serve as alternatives to antibiotics or the development of novel drugs to fight human infectious diseases. Moringa oleifera is a member of the Moringaceae family with nutritional and medicinal properties.&#x0D; Aim: To identify the bioactive constituents present in Moringa oleifera leaves using Gas chromatography-Mass spectrometry.&#x0D; Methodology: The bioactive compounds were extracted using the Soxhlet method of extraction and was analyzed using Gas chromatography mass-spectrometry.&#x0D; Results: The chromatogram of the GC-MS analysis used to identify the bioactive constituents present in the aqueous extract of Moringa oleifera leaves extract showed the presence of seventy-eight compounds. This includes 1H-Indene, 2-pyridinyl–methyl ester (9.82%), Anthracene, 9-(2-propenyl) (6.72%), 2,6- Lutidine 3,5- dichloro-4-dideiylthio (5.67%) and various other compounds which were identified as low level.&#x0D; Conclusions: The GC-MS analysis of Moringa oleifera showed the presence of bioactive compounds. These compounds are of high importance because they can be used in the production of new antimicrobials due to their valuable medicinal and therapeutic properties.

Comparative analysis of nutritional, antioxidant, and antibacterial properties of Moringa oleifera extract

People are increasingly more concerned with their lifestyle and health due to the significant shift in socioeconomic level in the modern era. People are aware of the negative consequences of manufactured items. Natural compounds derived from plants with fewer adverse effects are getting more attention. Moringa oleifera is an example of a tree with significant nutritional and therapeutic advantages. In the present study, mineral and macronutrient content and antioxidant capacity were evaluated at two stages of maturity (mature and tender leaves). The chemical analysis revealed that the protein concentration was higher, and the lipid concentration was lower. Regarding mineral content, calcium (Ca) and iron (Fe) have exhibited a higher degree of bioaccessibility, with potassium (K), sulfur (S), Ca, and Fe constituting the most prevalent elements. Using an established in vitro model, the antioxidant activities of Moringa oleifera leaf extracts were evaluated to comprehend the mechanism of pharmacological action. At 900 μg/ml, the aqueous extract of Moringa oleifera showed significant antibacterial activity. Based on the results of this study, Moringa oleifera leaf extracts exhibit considerable antioxidant activity and substantial protection against bacterial infection due to the presence of phenolic and flavonoids. Based on in vitro experiments, we aimed to determine if Moringa oleifera may be used as a potential antibacterial in therapeutic applications in light of the rising incidence of antibiotic resistance.

Bio synthesis and characterization of Cobalt oxide nanoparticles using aqueous extract of Moringa oleifera leaves

Recently the green synthesis of nanoparticles involving plant extract has attracted the attention of researchers. In this work we present an environmentally friendly, one step, ultra-fast, cost-efficient method for producing cobalt oxide nanoparticles using readily available Moringa Oleifera (Drumstick) extracts as reducing and capping agent. The bio-reduced cobalt nanoparticles were appropriately characterized. The cobalt oxide nanoparticles are characterized by UV-DRS, IR, powder XRD, SEM and EDX. To characterize the crystal phase identification of the nanoparticle, X-ray Powder Diffraction (Powder XRD) is used. Surface morphology was studied by SEM and EDX to determine the amount of cobalt present. The unique physicochemical characteristics of Cobalt oxide are believed to have increased medical applications when synthesized via environmentally benign methods free of toxic byproducts.

Parametrical Study for the Effective Removal of Mordant Black 11 from Synthetic Solutions: Moringa oleifera Seeds’ Extracts Versus Alum

Prior studies have examined the ability of Moringa oleifera (MO) seed extract, among other natural coagulants, to remove several types of dyes. MO has been proven to have a high capacity to remove some anionic dyes. The aim of the present study is to explore the possible use of aqueous and saline extracts of MO as biocoagulants for the removal of Mordant Black (MB11) from aqueous solution. Their performances were compared to that of aluminum sulfate (alum). To do so, various operating parameters were investigated such as coagulant dose (100–600 mg/L), pH (3–11), initial dye concentration (100–350 mg/L), sodium chloride concentration (0.2–2 M), and sedimentation time (15–90 min). The maximum percentages of MB11 removal were found to be 98.65%, 80.12%, and 95.02% for alum, aqueous extract of MO (MOPW), and saline extract of MO (MOPS), respectively, at around pH 6.5 and for coagulant doses of 400 mg/L (alum) and 500 mg/L (MOPW and MOPS). The coagulation-flocculation mechanism of biocoagulants was hypothesized to be adsorption and charge neutralization. The two biocoagulants (MOPW and MOPS) showed an interesting versatility towards pH counter to alum which was very sensitive to this parameter. pH variations were measured for the three coagulants and proven to be negligible for the biocoagulants. Faster sedimentation time was recorded when MOPW and MOPS were used, suggesting the existence of larger quickly settleable flocs. Considering their high coagulative capacity, rapid and cost-effective preparation, and eco-friendly character, MO extracts can be considered as powerful alternatives to aluminum sulfate in the remediation of MB11 from wastewaters.

Moringa oleifera decreases biomarkers of oxidative stress in a murine NASH model

This study aimed to evaluate the effect of Moringa oleifera aqueous extract on biomarkers of oxidative stress in a murine model of NASH. The characterization of the extract was performed by DPPH and ABTS spectrophotometric assays. Male C57BL/6J mice were randomly separated into two groups. 1) Standard diet (ND) (n = 5) (18% lipid) and 2) High fat (HF) diet (n = 10) (60% lipid and 42 g/L sugar in water of use), for 16 weeks. At the end of eight weeks, five HF group mice were divided into a subgroup, 3) Moringa Oleifera (HF + MO), 290 mg/kg/day p.o. for eight weeks. Malondialdehyde (MDA) levels were determined in liver homogenates and the transcriptome by microarray. Differences between groups were determined by ANOVA/Kruskal-Wallis test. The trial was approved by the research ethics committee. Moringa aqueous extract showed antioxidant capacity; DPPH values were 10081.4 0.3 and 22960.4 0.3 for ABTS. Hepatic MDA levels were increased in the HF group compared to the ND group (p<0.05) and decreased in the moringa-treated group (p<0.05). In the transcriptome, mRNAs involved in endoplasmic reticulum stress were underexpressed. An increase in MDA has been demonstrated in a murine model of NAFLD induced by a high-fat diet. In our study, Moringa administration reduces MDA production and gene expression of molecules involved in oxidative stress. MO treatment is a therapeutic alternative for the NASH spectrum of liver disorders. The resources used in this study were from the hospital without any additional financing The authors declare no potential conflicts of interest.