Effect of Aqueous extract of Moringa Olivera leaves( AEMOL) on some characteristics of ram epididymal sperms under cooling conditions

Abstract

The current study was conducted in the Graduate Studies Laboratory at the College of Agriculture, Al-Muthanna University, for the period from December 3, 2022 to March 9, 2023, to determine the effect of an aqueous extract of Moringa leaves on some characteristics of epididymal sperm of rams under cooling conditions for different periods. The aqueous extract of Moringa leaves was prepared and stored in the refrigerator ( 5 °C) until the time of use, the testicles were transferred to the laboratory less than an hour after the slaughter of the animal, the caudal epididymis was diluted with a Tris extender containing 0.03 concentration of aqueous extract of moringa olivera leaves.The results of this study showed a significant increase (P<0.01) in the percentage of live sperm, individual motility, and the plasma membrane integrity, while the adding of AEMOL leads to a decreasing of the percentage of abnormal sperms. Increasing the cooling hours of sperm leads to a significant decrease in the percentage of live sperm, individual motility, testing the integrity of the plasma membrane with an increase in the percentage of abnormal sperm. Using the aqueous extract of Moringa leaves at different times led to a significant effect on the individual motility, percentage of the plasma membrane integrity represented by the hypo-osmotic swelling test (HOST) , While the treatment did not significantly affect the percentage of live sperm and abnormal sperm. According to the results of this study we conclude that the aqueous extract of Moringa Oleifera leaves improves the characteristics of rams epididymal sperms preserved under cooling conditions for different periods