Effect of Aqueous Extract of Dill leaves (AEDL) on some Traits of Ram Epididymal Sperms under Cooling Conditions

Abstract

The current study was conducted in the Graduate Studies Laboratory of the College of Agriculture, Al-Muthanna University, for the period from December 3, 2022 to March 9, 2023, to determine the effect of an aqueous extract of dill leaves ( AEDL)on some properties of epididymal sperms of rams under cooling conditions of 5 °C for different periods. The aqueous extract of dill leaves was prepared and kept in the refrigerator until the time of use. The epididymal sperms were obtained from the testis of Rams that slaughters in the local slaughterhouse of Al-Muthanna Governorate, the testicles were transferred to the laboratory less than an hour after the slaughter of the animal, the caudal epididymis was diluted with a Tris diluent containing 3% concentration of aqueous extract of dill leaves. The results of using the aqueous extract of dill leaves showed a significant increase (P<0.01) in the percentage of live sperm, Individual motility, and a decrease in the percentage of abnormal sperms. While the use of the extract did affect the percentage of the integrity of acrosome represented by the hypo osmotic swelling test (HOST) at the 0 time of cooling preservation, The Increasing of the time of sperm cooling.leads to a decreases in the percentage of live sperm, HOST, individual motility, with an increase in the percentage of abnormal sperms. The use of the aqueous extract of dill leaves at different times resulted in a significant effect(P< 0.01) on the percentage of live sperm, individual motility, while it did not significantly affect the percentage of abnormal sperm, and there were no significant differences in the percentage of HOST. according to the results of this study We can conclude to adding the aqueous extract of dill leaves to Improve the physical traits of rams epididymal sperm include the live sperm and individual motility under cooling conditions for different periods.