Abstract
This research aimed to determine the effect of spermatozoa X and Y separation using swim-up method on the quality of etawah crossbreed goat’s spermatozoa. This research used 6 male etawah crossbreed goats aged between 18-24 months, which were collected the semen once a week using electroejaculator. Immediately after semen collection, the quality of semen was examined, and then grouped into three treatment groups. Group 1 was control group (P0) refers to the semen was not separated by swim-up method. Group 2 (P1) was the semen separated by the swim-up method for 5 minutes. Group 3 (P2) was the semen separated by swim-up method for 10 minutes. Each treatment was repeated six times. Parameters for semen quality examined were the percentage of spermatozoa motility, live spermatozoa, and abnormal spermatozoa. Data were analyzed by analysis of variance (ANOVA) one way pattern, followed by Duncan's multiple test. The results showed that the mean ± SD percentage of sperm motility on each treatment groups (P0; P1; P2) was 81.33±3.44, 89.67±3.21, and 90.00±3.10 %, respectively. The percentage of live spermatozoa on each group was 86.50 ± 2.07, 92.33 ± 2.08, and 91.83 ± 1.72%, respectively. The percentage of abnormal spermatozoa on each group was 13.00±2.53, 7.33±2.52, and 7.67±2.16%, respectively. Results of this research showed that the percentages of motility, live spermatozoa, and abnormal spermatozoa after separation by swim-up method were significantly different (P0.05) compared to without separation. It can be concluded that the separation of spermatozoa using swim-up method can improve significantly (P0.05) the percentage of live and motility of spermatozoa and decrease the percentage of abnormal spermatozoa of etawah crossbreed goats.Key words: Etawah crossbreed, spermatozoa quality, swim-up method
Highlights
Pengembangan peternakan di Indonesia khususnya dalam rangka meningkatkan populasi ternak, untuk mencukupi kebutuhan konsumsi dalam negeri, perlu didukung oleh berbagai faktor
Salah satu upaya pemisahan spermatozoa berkromosom X dengan spermatozoa berkromosom Y dapat dilakukan dengan cara metode swim up dalam medium isotonis
Pemisahan spermatozoa dengan swim up didasarkan atas perbedaan motil atau kecepatan berenang spermatozoa berkromosom Y dengan spermatozoa berkromosom X keluar dari pelet menuju ke permukaan media (Parrish et al, 1995; de Jonge et al, 1997; Cleassens et al, 1998; Yuliani, 2000)
Summary
Eskayanti Pasaribu, dkk menginseminasikan seekor betina berahi dengan spermatozoa yang sudah dipisahkan (spermatozoa berkromosom X dan spermatozoa berkromosom Y). Hasil penelitian Mirajuddin (1997) membuktikan bahwa pemisahan spermatozoa kambing PE dengan metode swim up selama 10 menit, dapat menghasilkan kualitas spermatozoa dan setelah inseminasi pada induk betina didapatkan anak berjenis kelamin jantan sebanyak 100% dan berjenis kelamin betina sebanyak 0%. Yuliani (2000) melakukan pemisahan spermatozoa sapi bali dengan metode swim up selama 30-45 menit dalam medium EBSS, diperoleh populasi spermatozoa berkromosom Y sebanyak 82,40±4,52% dan populasi spermatozoa berkromosom X sebanyak 17,60±3,15% pada lapisan atas, setelah fertilisasi in vitro (FIV) diperoleh 82,45±6,67% embrio berjenis kelamin jantan dan 17,55±3,83% embrio berjenis kelamin betina. Hasil yang hampir sama juga dilaporkan oleh Dasrul et al (2009) pada spermatozoa kambing Boer yang dipisahkan dengan metode swim up selama 5 menit, dengan pengukuran diameter kepala diperoleh pada lapisan atas populasi spermatozoa berkromosom Y sebanyak 76,20% dan populasi spermatozoa berkromosom X sebanyak 23,80%. Sampai saat ini kajian tentang pengaruh pemisahan spermatozoa X dan Y dengan metode swim up terhadap kualitas spermatozoa kambing PE belum pernah dilakukan
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