Abstract
This study aimed to determine the effect of sperm washing by swim up and centrifugation in isotonic medium on sperm quality of aceh bull. In this study, fresh semen from healthy male aceh bull aged 3-4 months was collected using artificial vagina. Immediately after semen collection, fresh semen quality was examined macroscopically and microscopically. Subsequently, sperm washing was performed by centrifugation and swim up in sperm washing medium. Group 1 (P0) as control group, cement washed with isotonic solution (andromed medium: saline solution) with ratio of 1:8. 2. Group 2 (P1), cement was separated by centrifugation method, group 3 (P2), all cement was separated by swim up method then examined the sperm quality sperm washing results. Each treatment was repeated 5 times. Quality parameters measured were the percentage of spermatozoa motility, sperm viability, and plasma membrane integrity intact spermatozoa. Data were analyzed with analysis of variance one-way pattern, followed by Duncan's multiple test. The results showed the mean ± SD percentage of sperm motility of each treatment group (P0; P1; P2) respectively amounted to 72.00±3.74, 66.40±4.77, and 73.60±3.29%. The percentage of viability was 72.00 ±3.74%, 66.40±2.88%, 71.80±2.17%. The percentage of plasma membrane integrity is intact spermatozoa was 68.20±1.79%, 57.20±3.77%, 69.00±2.00%. Results of this study showed that the percentage of motility, live spermatozoa and plasma membrane integrity intact after separation by swim-up method were significantly different (P <0.05) compared with no separation.Key words: spermatozoa quality, aceh bulls, centrifugation, swim up
Highlights
Upaya meningkatkan penampilan hewan ternak sapi dengan daya produksi yang bermutu tinggi dapat ditempuh melalui usaha penyediaan dan menyebarluaskan bibit unggul ternak tersebut, terutama penggunaan pejantan-pejantan unggul, sehingga kontribusi dari ternak sapi terhadap penyediaan pangan asal ternak akan lebih dirasakan
Group 2 (P1), cement was separated by centrifugation method, group 3 (P2), all cement was separated by swim up method examined the sperm quality sperm washing results
The results showed the mean ± SD percentage of sperm motility of each treatment group (P0; perlakuan 1 (P1); perlakuan 2 (P2)) respectively amounted to 72.00±3.74, 66.40±4.77, and 73.60±3.29%
Summary
Dilaporkan oleh beberapa peneliti pencucian spermatozoa dapat dilakukan dengan beberapa cara diantaranya melalui sentrifugasi (Dasrul, 1999), filtrasi kolom sephadeks, dan swim up (Mirajuddin, 1997; Yuliani, 2000). Telah terbukti bahwa pencucian spermatozoa dengan sentrifugasi dalam medium isotonis seperti earle’s balance salt solution (EBSS) akan meningkatkan persentase spermatozoa motil dengan morfologi yang normal (Dasrul, 1999; Sterckx et al, 2006, Kaewnoonual et al, 2008; HeeJun Chi et al, 2011). Pemisahan spermatozoa dengan swim up didasarkan atas perbedaan kecepatan renang spermatozoa ke luar dari pelet menuju ke permukaan media (De Jounge et al, 1997; Mirajuddin, 1997; Yuliani, 2000). Hasil penelitian Yuliani (2000) pemisahan spermatozoa sapi bali dengan swim up dapat meningkatkan perolehan persentase motilitas dan viabilitas spermatozoa serta menurunkan jumlah spermatozoa abnormal
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