Changes of Blood Biochemical Parameters During Immune Deficiency Prevention with an Extract of Moringa oleifera in Wistar Rats

Abstract

The aim of this work was to determine the evolution of some biochemical parameters during a preventive immunostimulation assay of the total aqueous extract of Moringa oleifera leaves in rats. To do this, 48 albino rats including 24 males and 24 females divided into 12 groups of weight between 107 g and 140 g were used. The different doses of the aqueous extract of Moringa oleifera leaves 200 mg/kg bw, 400 mg/kg bw, 800 mg/kg bw and 1600 mg/kg bw respectively were compared with controls subjected to distilled water and 50 mg/kg bw of levamisole for 14 days. At the end of this 14-day treatment, immunosuppression was performed by administration of 5 mg/kg bw intraperitoneally dexamethasone in all rats grouped for three days twice daily. Then a two-week observation was made to assess the behavior of some biochemical parameters after administration of dexamethasone. During the experiment five samples were taken on day 1, day 14, day 17, day 21 and day 28. These different samples allowed the determination of biochemical and nutritional parameters. These samples were used to determine urea, creatinine, transaminases (AST/ALT), PAL, LDH, total cholesterol, HDL, LDL, RRC, CA, RC, IAP, triglycerides and finally nutritional parameters (albumin and total proteins).