Interaction between aqueous extract of Moringa oleifera leaves and rabeprazole in treatment of ethanol induced gastric ulcer in Wistar rats

Abstract

Background: The effect of concomitant use of herbs and conventional drugs is not widely known because it hasn’t been a common practice especially among the elite and those resident at the urban centers but very common among the rural duelers for co-administered herbs/supplements and conventional drugs. This study was carried out to investigate the interaction between moringa oleifera and rabeprazole in gastric acid secretion. Methods: Twenty (20) adult Wistar albino rats of both sexes weighing 300-450 g were randomly allocated into four (4) groups of five (5) animals per group. Group 1 served as the control and was given normal saline without administration of acid alcohol. Animals in group 2 were given acid alcohol (0.25 ml 0f 36% HCl + 75% ethanol 0.25 ml/100 g body weight). Animals in group 3 were given 50 mg/100 g bw of Moringa oleifera 1 hour before acid alcohol was administered. Group 4 were given: Rabeprazole 20 mg /kg body weight + acid Alcohol (36% HCL 0.25 mls+ 75% Ethanol (0.25 mls). A 30% isoflorane (inhalational anesthesia) by API Manufacturer with FDA, UK, marketed by Macfes medical store, high level Markurdi, Benue State, Nigeria. And 3.5% isoflurane (of the mixture of isoflurane 30%) at 100% oxygen was soaked in a cotton wool and dropped in clean and covered plastic container, and was used to anesthetized the rats, for the purpose of the aqueous extracts administration through a gastric fistula created by a surgical procedure. 10 minutes after aqueous extracts administration, aliquot samples were collected over 4 hours. Gastric acid secretion was measured by titrating the aliquots to a phenolphthalein endpoint. Results: showed that Moringa Olefera significantly delayed the time of onset of action of rabeprazole when co-administered. Moringa alone was a better inhibitor of gastric acid secretion than moringa co-administered with rabeprazole. Conclusion: Moringa delays the time of onset of action of Rabeprazole when co-administered.