Moringa Oleifera Aqueous Extract Research Articles

Acetaminophen induces liver injury and depletes glutathione in mice brain: Prevention by Moringa oleifera extract

Acetaminophen (APAP) overdose is among the leading causes of acute liver failure worldwide. Moringa oleifera is used as food additive and, as phytomedicine for its antioxidant, analgesic, anti-pyretic, anti-inflammatory, immuno-modulatory and hepatoprotective properties. The present study investigated the effect of Moringa oleifera aqueous extract on acetaminophen-induced acute liver injury and glutathione depletion in mice brain. Moringa oleifera leaf aqueous extract (100 and 200 mg/kg, p.o.), distilled water and ascorbic acid (50 mg/kg, p.o.) were administered to mice 1 and 12 h before acetaminophen (500 mg/kg, p.o.). Mice were sacrificed 6 h after APAP treatment. Aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) serum activities; malondialdehyde (MDA) and nitrite liver level, tumor necrosis factor alpha (TNF-α or TNF) and interleukin-1β (IL-1β) serum levels were assessed. Reduced glutathione (GSH) was evaluated in brain and liver tissues. Histological analyses of liver section were performed by hematoxylin/eosin (H&E) staining. APAP administration induced liver injury with significant (P < .01) depletion of liver and brain GSH level. ALT, ALP and AST serum activities (P < .05), MDA and nitrite liver content, TNF and IL-1β in the serum were increased (P < .05). Moringa oleifera extract significantly (P < .05) reduced liver injury, inhibiting ALT, AST and ALP serum activities. TNF and IL-1β serum levels, nitrite, and MDA liver content were significantly (P < .05) reduced by M. oleifera extract, compared to APAP-treated mice. GSH depletion in brain and liver was inhibited by M. oleifera extract. Histological injuries (vacuolization and inflammation) by acetaminophen were remarkably prevented by M. oleifera administration. Results of the present study revealed that M. oleifera leaf extract strongly protects mice against acetaminophen hepatotoxicity, exhibiting antioxidant and anti-inflammatory effects. The present results support ethnopharmacological uses of M. oleifera for the treatment of liver diseases.

Incubation period induced biogenic synthesis of PEG enhanced Moringa oleifera silver nanocapsules and its antibacterial activity

Human pathogenic diseases are on the rage in the list of enfeebling diseases globally. The endless quest to salvage these drug-resistant pathogens ravaging our system through various therapies still posts serious challenge. This study engaged a biogenic synthesis that is benign, facile, biocompatible, cost-effective and eco-friendly to synthesized silver nanocapsule (AgNCs) via Moringa oleifera aqueous extract under incubation control. The flavonoid-kaempferol, phenolic-chlorogenic acid and tannin components of MO acted as the potential stabilizing and reducing agent in the formation of AgNCs. The formulated AgNCs was further functionalized with PVA, PVP and PEG for biocompatibility and dispersion enhancement. Various characterization techniques were used to determine the properties of AgNCs formulated. The absorbance due to the color change was observed by the UV-Visible spectroscopy with surface plasmons resonance peak between 425 and 455 nm. The Fourier transform infrared spectroscopy (FTIR) shows the various functional group responsible for the biogenic synthesis of AgNCs. The X-ray spectroscopy analysis shows a single phase cubic structure of AgNCs formed. The Scanning electron microscopy (SEM) image shows a rod-like nanocapsule of uniform grains. The antibacterial potency of AgNCs was proven against gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Coliform). The AgNCs inhibited the growth of the three human pathogens with Coliform showing the highest activity to the AgNCs with a minimum inhibitory dose of 15 μg/mL. It is noteworthy that the bacterial strains show functional susceptibility to the AgNCs at lower concentrations compared to the conventional antibacterial drugs. Consequently, AgNCs serve as an enhanced substitute for the conventional antibacterial drugs in therapeutic biomedical field sequel to its pharmacodynamics against the bacterial strains.

Inhibitory activity of Lactobacillus plantarum ATCC 8014 fermented milk combined with aqueous extract of Moringa oleifera leaves against Streptococcus mutans

The growth inhibitory activity of Lactobacillus plantarum ATCC 8014 (Lp) fermented milk combined with aqueous extract of Moringa oleifera leaves against Streptococcus mutants has been studied. The preparation is potential developed as healthy food due to its nutritional value as well as its beneficial activities. The well agar diffusion method was applied for the antibacterial assay on nutrient agar media and local strain of Streptococcus mutants was used as a bacterial test. This species often causes infectious in the teeth. Effectiveness of the fermented milk and its combination with aqueous extract preparations against the bacterial test were assessed by minimum inhibitory concentration (MIC) for the potency indication. The aqueous extract of Moringa oleifera leaves was prepared as infuse like according to community daily consumption. Concentration of the extract used in the formulation was higher than the MIC. The result showed that the MIC of the aqueous extract of Moringa oleifera leaves and the L. plantarum fermented milk were 20% and 35% with growth inhibition zone diameter of 11.70 ± 0.28 mm and 12.02 ± 0.83 mm, respectively. The combination of fermented milk and aqueous extract was prepared in various ratio concentration (1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, and 9:1). The optimum ratio of the combination was 2:8 with growth inhibition zone diameter of 16.53 ±0.32 mm and the MIC value at optimum ratio was 45% with inhibition zone diameter of 11.90 ± 0.86 mm.

Improvements of alveolar bone healing using Moringa oleifera leaf powder and extract biomimetic composite: an experimental study in dogs

Alveolar ridge preservation techniques and biomaterials were extensively used in the past few years. The rate of new bone formation is the concept in healing sockets. The faster the rate the lesser the alveolar bone resorption. Resorption is fastened in the first three months after extraction. Wide range of biomaterials were available, the choice of an ideal material was confusing.Objective: Evaluate the effectiveness of both Moringa Oleifera (MO) leaf powder and extract mixture locally and MO leaf extract repeated injections in fresh extraction sockets of dog. Moreover, identifying bioactive phenolic compounds in dried leaves.Materials and Methods: Eight mongrel dogs were included in this study; two animals were used as control. Six test animals were divided into two groups according to biopsy time (3 and 5 weeks). Each of these groups includes three animals. All animals were subjected to extraction of the third premolar bilaterally, both sides of the test animals (the six) were treated with Moringa Oleifera leaf powder (0.7 gm) and water extract (1ml) mixture. The right side only was subjected to repeated injections of Moringa Oleifera leaf water extract (½ ml) for two times (1st week and 2nd week postoperatively for all groups). Trephine bur was used to take biopsy for the purpose of histopathologic examination by H&E and Masson Trichrome stain to quantify new bone formation, and number of inflammatory cells.Results: Bone area percent showed the highest mean value in the right side at 3 weeks and 5 weeks. ANOVA test revealed no significant difference between groups (P=0.43) at 3 weeks, while the difference was significant at 5 weeks (P=0.00). Mean inflammatory cells count were significantly higher mean value in control group at 3 weeks and 5 weeks (P=0.00). Conclusion: Two injections of moringa oleifera aqueous extract of 11.7% concentration improved bone area present significantly at 5 weeks and decreased inflammatory cells at all test periods.

Study on the ameliorating effect of aqueous extract of Moringa oleifera on Ganga water

The quality of surface water is deteriorating day by day due to various anthropogenic activities. Ganga water is assumed polluted almost in the entire strech of river. The medicinal plants may be useful for amelioration of pollution. The present work deals with the study of several chemical characteristics as well as microbial examination of water samples taken from few sites of Ganga river namely Krishna Ghat, Kali Ghat and N.I.T Ghat (Gandhi Ghat) at Patna. The study was foccused on the evaluation of antimicrobial effect of Moringa oleifera (sahjan) leaves extract in the Ganga water. The aqueous leaves extract of different concentrations (0.1%, 0.16%, 0.23%, 0.3%) showed ameliorating results on pH and chloride content of water as well as microbial activity. Significant ameliorating results were observed on pH and chloride content of water. pH was reduced from 8.1 to 7.4 and chloride was reduced from 82.36 mg/l to 48.14 mg/l under plant extract treatment. Colony Forming Unit (CFU) was assessed by standard plate count (SPC) method and was found to be 468 CFU/ml for raw water, which was reduced to 143 CFU/ml when treated with plant extract. Similar decreasing trend was observed in case of MPN of coliform. So extract of Moringa oleifera may also be applied to purify drinking water. Morever it will be cheaper for the people in comparision to other purification devices.

Moringa oleifera leaf extract lowers high blood pressure by alleviating vascular dysfunction and decreasing oxidative stress in L-NAME hypertensive rats

BackgroundEnhancing relaxation of resistance arteries and decreasing oxidative stress by using natural products are potential strategies for prevention and treatment of hypertension. PurposeThis study investigated whether aqueous extract of Moringa oleifera leaves (MOE) could alleviate Nω-nitro-L-arginine-methyl ester (L-NAME)-induced high blood pressure via modulation of vascular function and antioxidant properties. MethodsAn experimental hypertensive model was established by administration of L-NAME (50 mg/kg/day) in drinking water to male Wistar rats for 3 weeks. Arterial pressure was measured indirectly by tail-cuff plethysmography and directly via femoral artery catheterization. Vasoreactivity of isolated rat mesenteric arterial bed was determined by the changes in perfusion pressure detected by a pressure transducer. Vascular superoxide anion (O2•−) production was determined by lucigenin-enhanced chemiluminescence. Other biochemical measurements including malondialdehyde (MDA) level, superoxide dismutase (SOD), and catalase (CAT) activities were measured by colorimetric assay. ResultsL-NAME-treated rats developed significantly increased blood pressure and heart rate. Concurrent oral treatment with MOE (30 and 60 mg/kg/day) could decrease the high blood pressure and tachycardia in a dose-dependent manner. MOE reduced the impairment of acetylcholine-induced relaxation and decreased the hyperreactivity of adrenergic-mediated contraction in response to periarterial nerve stimulation and phenylephrine in isolated mesenteric arterial beds. In addition, MOE exhibited antioxidant effects in the hypertensive rats, as indicated by suppression of vascular O2•− production, decrease of plasma and thoracic aorta MDA levels, and increase of antioxidant activities of SOD and CAT. Moreover, MOE (0.001–0.3 mg) produced a dose-dependent relaxation in methoxamine pre-contracted arterial beds isolated from L-NAME hypertensive rats, which was abolished by endothelium denudation. ConclusionThese findings suggest that the antihypertensive effect of MOE in L-NAME-hypertensive rats may be mediated by alleviating vascular dysfunction and oxidative stress and promoting endothelium-dependent vasorelaxation. MOE may be potentially useful as a natural product against hypertension.

Effects of aqueous extract of Moringa oleifera seed on cadmium-induced reproductive toxicity in male Wistar rats.

Moringa oleifera seeds have been reported to increase sexual activity of male rats but its mechanism of action remains unknown. Cadmium is a heavy metal that induces reproductive toxicity. To elucidate its possible mechanisms of action, the effects of aqueous extract of Moringa seeds (AEMS) on Cadmium-induced reproductive damage were investigated. Thirty male rats (180-200g, n=5) were grouped as follows: Control, Cadmium (2mg/kg), AEMS (100 and 500mg/kg), Cadmium+100mg/kg AEMS and Cadmium+500mg/kg AEMS. Single intraperitoneal dose of Cadmium was administered while AEMS was given daily (p.o) for 6 weeks and thereafter sacrificed by cervical dislocation. Sperm variables were examined microscopically while serum was analysed for sex hormones and antioxidants, testicular and epididymal total protein from tissue homogenate. Data were analysed using Students t-test and ANOVA. Cadmium caused significant decrease in sperm variables. The AEMS decreased sperm motility, count and testosterone. These declines were dose dependent. Total testicular protein was significantly lowered by AEMS but total epididymal protein was not affected. Serum catalase and SOD was reduced by AEMS while MDA increased. Moringa seeds may exhibit male reproductive toxicity, as observed from its deleterious effect on androgen and sperm variables. It failed to prevent cadmium-induced alteration in reproductive variables and serum antioxidants.

Combined use of coagulation (M. oleifera) and electrochemical techniques in the treatment of industrial paint wastewater for reuse and/or disposal

In this work, water-based paint (WBP) wastewater was treated using a natural coagulant, Moringa oleifera aqueous extract (MOAE), fortified with Ca2+ (from nitrate and chloride salts). In order to improve the quality of the treated wastewater and render it suitable for disposal, an electrolytic flow process was associated with the wastewater treatment using a filter-press reactor with a boron doped diamond (BDD) electrode. The feasibility of the treatment was evidenced by the reuse of the treated wastewater in the production of a new paint (manufactured by the company supplying the raw wastewater), whose quality was compatible with the water used by the manufacturer. The best conditions for the coagulation-flocculation process involved the use of 80 mL of MOAE (50 g/L of MO and 0.125 mol/L of Ca2+) for every 1.0 L of wastewater at pH 6.5. The limiting current density (35 mA/cm2) and an electrolysis time of 90 min (charge passed of 3.68 A h/L) were used in the electrochemical treatment. Biotoxicity assays using the brine shrimp Artemia salina revealed that the mortality (in %) of microcrustaceans was reduced from 100% (raw wastewater) to only 11% at the end of the electrolysis process, in addition to eliminating the strong odor and 85% of the organic load. Moreover, microbiological tests showed that the number of mesophiles decreased by more than six orders of magnitude and there was no growth of thermotolerant coliforms (TC).

In-vitro and in-vivo determination of hydroxyl radical scavenging activity (hrsa) of fractions of aqueous extract of moringa oleifera leaves (aemol)

In-vitro and in-vivo determination of hydroxyl radical scavenging activity (hrsa) of fractions of aqueous extract of moringa oleifera leaves (aemol)

Efficacy of Moringa oleifera Aqueous Extract in Inhibiting Tamoxifen®-Induced Physiological Hepatic Deterioration in Male Albino Rats.

Tamoxifen citrate (TAM) is a widely used drug in breast cancer treatment. It showed a degree of hepatic carcinogenesis. The purpose of this study was to elucidate the antioxidant capacity of Moringa oleifera aqueous extract (MAE) against TAM-induced liver injury. A model of liver injury in male rats was done by orally administration of TAM in a dose of 3mg/Kg/3days for consecutive six weeks to evaluate the drug-toxicity in combination with MAE in a dose of 300mg/Kg/day for similar period. The model of TAM-intoxication elicited significant elevation in serum alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT) activities as well as hepatic levels of the oxidative stress markers (MDA and NO),lipid profiles and inflammatory marker(TNF-α) associated with a significant depletion of anti-oxidative marker (GSH).The oral administration of MAE in combination with TAM-intoxicated rats, resulted in significant improvements in ALAT,ASAT and anti-oxidative marker (GSH)with significant decrements in MDA, NO and lipid profiles. The data obtained from this study speculated that MAE has the capacity to scavenge free radical and can protect against oxidative stress induced by TAM intoxication. Supplementation of MAE could be useful in alleviating tamoxifen-induced liver injury in rats.

Hyperglycaemia and Oxidative Stress in Wistar Albino Rats: Effects of Aqueous Extract of Moringa oleifera (Lam) Leaf

Hyperglycaemia and Oxidative Stress in Wistar Albino Rats: Effects of Aqueous Extract of Moringa oleifera (Lam) Leaf

Uso de sementes de Moringa Oleífera (LAM) no tratamento de efluente têxtil

The seed extracts of Moringa oleifera has been studied as alternative coagulating agent to chemicals commonly used in order to minimize costs while reducing environmental impacts and human health arising from traditional processes of wastewater treatment. This work studied the performance of the aqueous extract of Moringa oleifera seed in the removal of four different types of textile dyes classified as dispersed, acidic, basic and reactive. Studies with aqueous and saline solutions were performed aiming to improve performance in the removal of textile dyes, and stability testing on the storage time of the extract of Moringa oleifera. Studies were performed using spectrophotometric analysis by means of calibration curves of textile dyes in an aqueous medium.The results showed that the seed extract of Moringa oleifera was quite efficient in the removal of textile dyes, except for the cationic type. Regarding the behavior of textile dyes in aqueous solution and the saline extract were not found significant differences in terms of removal. Other promising results were obtained to evaluate coagulant activity of the extract up to three days storage at room temperature. This fact is important from the operational aspect arising from the use of the extract on a large scale: the possibility to prepare a larger amount of extract, without loss of coagulant activity may enable the utilization of this resource

Histological Effects of Aqueous Extract of Moringa oleifera on the Liver Tissues of Wistar rats fed with High Fat Diet

Moringa oleifera belongs to family of moringaceae and is considered as one of the world’s most useful trees, as almost every part of the plant can be used for either as food, or therapeutic purposes. Fatty diet is a significant factor in the pathogenesis of non-alcoholic fatty Liver disease (NAFLD). The study was designed to examine histological effects of aqueous extract of Moringa oleifera on the liver tissues of wistar rat fed with high fat diet. Twenty five adult rats were divided into five groups of five animals each .While group A received distilled water daily only, groups Band C received aqueous extract of Moringa oleifera at doses of 200 mg/kg body weight and fat high diet (30% w/w of the total mash feed) respectively for a duration of seventy days. Others (groups D and E) received aqueous extract of Moringa oleifera at doses of 200 mg/kg body weight and fat high diet for a duration of seventy days. Histology of the liver of the rats fed with high fat diet exhibited significant changes in the architecture of liver tissue. The changes include micro and macro vascular steatosis, increased fatty infiltration, inflammation, sinusoidal dilation, degeneration of veins and vacuolization as compared to normal liver histology. Treatment with 200 mg/kg extract of Moringa oleifera significantly attenuated these effects imposed by high fat diet as compared to the control group. Therefore demonstrated that daily administration of Moringa oleifera leaves extract to rats for a period of 70 days may reverse the formation of hepatic steatosis in non-alcoholic fatty liver disease.

An experimental study to evaluate the anti-inflammatory effect of moringa oleifera leaves in animal models

Background: Inflammatory diseases are a major cause of morbidity and disability of work force throughout the world. The treatment of inflammation with standard steroidal and non-steroidal anti-inflammatory drugs shares the risk of toxicity on various organ systems. Moringa oleifera, an herbal plant has been claimed to be effective in the treatment of various types of inflammatory conditions. However, there is lack of scientific studies to ratify these claims. Therefore, the present study was undertaken to explore the anti-inflammatory activity of aqueous extract of leaves of Moringa oleifera (AEMO) in experimentally induced inflammation in albino rats.Methods: The study was commenced after obtaining approval from Institutional Animal Ethical Committee using AEMO leaves in Albino wistar rats (150-200 gm) of either sex. The anti-inflammatory activity was evaluated using carrageenan induced paw edema model, cotton pellet induced granuloma method and formaldehyde induced paw edema method. For each set of experiment, animals were divided in three groups of six animals each. In each experiment, 1st group was given normal saline (5 ml/kg/day), 2nd group was given standard anti-inflammatory drug dexamethasone (0.5 mg/kg/day) and 3rd group was given Moringa oleifera (200 mg/kg/day).Results: Aqueous extract of Moringa oleifera leaves at dose of 200 mg/kg, p.o. exhibited the significant anti-inflammatory effect in all the models used in this study.Conclusions: It can be concluded from our study that aqueous extracts of Moringa oleifera leaves possess anti-inflammatory activity.

Utilization of Aqueous Extract of Moringa oleifera for Production of Functional Yogurt

The aqueous extract of Moringa oleifera contained a bioactive compounds namely tannins, flavonoids, saponin, alkaloids and terpens. The aqueous extract has high content of total phenolic (340.82 mg/g as gallic acid), In addition to IC 50 of Moringa oleifera extract was 75.82 µg/ml, and therefore the addition of Moringa oleifera extract to yogurt enhances the nutritional value of the resultant yogurt. The aqueous extract exhibited a antibacterial activity against Gram positive bacteria (S. aureus , E. faecalis and B. cereus), in contrast to Gram negative (E.coli and S. Typhimurium) more resistance to extract of Moringa oleifera. The total solid of yogurt is directly proportional to addition of extract content. A slightly decrease in pH was noticed in yogurt enriched aqueous extract. The use of Moringa oleifera extract at mentioned concentration rates increased significantly curd tension and WHC, in addition to susceptibility of synersis was decrease. The addition of aqueous extract of Moringa oleifera enhanced sensory properties of the resultant yogurt. Acetaldehyde and diacetyl increased in yogurt with Moringa oleifera extract, compare with control. Hardness, Adhesiveness and gumminess were significantly higher (P> 0.05) in experimental yogurt made by using different concentration of aqueous extract of Moringa oleifera than control. However, the cohesiveness and springiness were significantly lower in samples of experimental yogurt made with different concentration of aqueous extract of Moringa oleifera than control.

Preventative and Curative Effect of Moringa oleifera Aqueous Extract to Ensure Safe Natural Antimicrobials Targeting Foodborne Pathogens

The interest in biopreservation of food has prompted the quest for new natural antimicrobial compounds from different origins including plants. Medicinal plants are moving from fringe to mainstream use with a greater number of people seeking for remedies and health approaches from side effects caused by synthetic chemicals. This has aggravated the search for antimicrobials from plants sources. In this study, aqueous extract of Moringa oleifera was screened for antimicrobial activity against five foodborne bacterial isolates (Bacillus cereus, Staphyllococcus aureus, Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa). As well as, against five fungal and yeast strains, including Aspergillus niger, Aspergillus flavus, Penicillium italicum, Rhizopus stolonifer and Candida albicans. Various methods for better antimicrobial activity were established. Minimum Inhibitory Concentration (MIC) and time-kill assays were also determined. The Moringa oleifera aqueous extract exhibited antimicrobial activity towards all tested microorganisms with markedly stronger anti-Gram positive bacteria activities were 26.2 ± 0.55 and 24.26 ± 0.71 (mm) against B. cereus and S. aureus, respectively, while the anti- Gram negative bacteria halos of inhibition ranged from 18.230.81 to 21.73 ± 0.55 as an organism specific. However, the Moringa oleifera aqueous extract showed strong antifungal activity in exception C. albicans, the effect was concentration dependant in a range from 15.2 ± 0.79 to 35.7 ± 0.63. Based on MICs values Moringa oleifera aqueous extract exhibited a wide spectrum of actions against both two Gram-types bacteria and tested fungal/yeast strains and the concentration values ranged from 1.562 to 12.5 mg/ml against bacteria depending on Gram character and ranged from 1.562 to 25 mg/ml against the panel of fungi and yeast tested. The MICs values were organism specific and concentration dependent. Based on the time-kill assay Gram-negative bacteria required longer time of exposure than the Gram-positive one. Interestingly, Moringa oleifera aqueous extract at sub-inhibitory concentrations showed strong activity in smashing up foodborne bacterial biofilm as a dose dependent manner. Scanning Electron Microscopy (SEM) images showed the morphological damages and strong destructive effects against both Gram-positive and Gram-negative bacteria. Finally, Moringa oleifera aqueous extract has good potential for controlling growth of foodborne pathogenic and spoilage microorganisms and open new fields in food biopreservation. As well as, breaks the increasing prevalence of multi-drug resistant strains of bacteria and the recent appearance of strains with reduced susceptibility to antibiotics raised the specter of ‘untreatable’ bacterial infections and adds urgency to the search for new infection-fighting strategies.

Effect of aqueous extract of Moringa oleifera leaves on pharmacological models of epilepsy and anxiety in mice

ObjectiveAmong the psychiatric co-morbidities in epilepsy, anxiety disorders are highly frequent and have profound influence on the quality of life of epilepsy patients. Moringa oleifera Lam. (Moringaceae) is used in traditional medicine to treat various ailments including anxiety and epilepsy. However, no scientific evidence exists to support its use. We studied antiepileptic and anxiolytic activities of aqueous extract of Moringa oleifera Lam. leaves (AEMO). MethodsAntiepileptic activity was evaluated using pentylenetetrazole (PTZ) induced seizure and maximum electroshock (MES) induced seizure test and anxiolytic activity was evaluated using elevated plus maze, light/dark box and hole board test. ResultsIn present study, AEMO (250, 375 and 500mg/kg, i.p.) demonstrated significant antiepileptic and anxiolytic effects. To study involvement of GABA in anxiolytic and antiepileptic activity of AEMO, we also evaluated effect of AEMO on Baclofen induced catatonia, a GABA mediated behavior, wherein AEMO significantly potentiated (preponed) baclofen induced catatonia, which is suggestive of its GABA mimetic action. ConclusionThus, it may be concluded that aqueous extract of M. oleifera possess anxiolytic and antiepileptic effects possibly mediated via of GABA mimetic action and these findings authenticate the traditional claims about use of Moringa oleifera in treatment of epilepsy and anxiety.

Effects of Aqueous Extract of Moringa oleifera leaves on Epidermal Wound Healing in Domestic Rabbit

ABSTRACT A study was undertaken to assess the healing properties of Moringa oleifera leaf extract on epidermal wounds in rabbits. Twelve adult rabbits were divided into two equal groups of six. A pair of wound measuring 2cm x 2cm each was created on each rabbit. The wound on the rabbits in the experimental group were treated with Moringa oleifera aqueous leaf extracts while those in the control group were cleaned with distilled water daily. Wound contraction was measured daily with days 5, 9 and 12 representing the inflammatory, proliferative and maturation phases of wound healing respectively. Blood samples were collected on days 0, 5, 9 and 12 for analysis. Moringa oleifera leaf extract-treated animals had faster rates of wound contraction and healing with shorter days of scab fall off within 7 days; increases in packed cell volume, mean corpuscular volume, lymphocyte and neutrophil counts (P

Effects of Water Crude Leaf Extract of Moringa Oleifera Lam (Moringaceae) on Normotensive Rat Blood Pressure and Isolated Duodenum

Moringa oleifera is used in folk medicine for the treatment of many ailments among which, hypertension and intestinal disorders. The aim of this study was to investigate the hypotensive and/or antihypertensive activity of Moringa oleifera leaf extract on normotensive anaesthetized rats by direct measure of the arterial pression and in the other hand, to determine its antispasmodic effects on isolated rat duodenum. Intravenous administration of aqueous leaf extract of Moringa oleifera (EAMO 5mg/kg -75 mg/kg body weight), caused a transitory dose-dependent fail in the mean arterial blood pressure of normotensive anaesthetized rats (p< 0.05). Moreover, hypertensive arterial blood pressure induced by phenylephrine 100µg/kg rat body weight, was completely abolished by aqueous extract of Moringa oleifera 10mg-40mg/kg body weight. The studies on isolated rat duodenum smooth muscles demonstrated that the aqueous extract of Moringa oleifera produced a dose dependent relaxation of these muscles (p

Lead-Induced Damage on Hepatocytes and Hepatic Reticular Fibres in Rats; Protective Role of Aqueous Extract of &amp;lt;i&amp;gt;Moringa oleifera&amp;lt;/i&amp;gt; Leaves (Lam)

This study was designed to evaluate the hepatoprotective and ameliorative effects of aqueous extract of Moringa oleifera (MO) leaves on the histologyof liver and hepatic reticular fibres integrity of adult Wistar rats following lead-induced hepatotoxicity. Twenty four adult Wistar rats, weighing 180 - 220 g, randomly assigned into four groups of six animals each were used for the study. Lead and Moringa oleifera were given orally to the rats. 24 hours after the last administration, animals were sacrificed, blood obtained by cardiac puncture and liver excised, fixed in 10% phosphate buffered formalin for histological and histochemical analysis. The activities of aspartate amino transferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) were used as markers of hepatotoxicity and catalase (CAT) activity were used as marker to evaluate the anti-oxidant status of the tissue. Result showed that lead treatment increased markers of hepatic damage (ALT, AST and ALP) and decreased CAT activities. Histological studies reveal alterations of hepatic structure including hepatocytic vacuolations, sinuosoidal congestion and loss of reticular fibres following lead treatment. Treatment with MO prevented and reversed lead induced hepatic damage. In conclusion, this study shows that Moringa oleifera leaf extract has an appreciable ability to prevent hepatotoxicity caused by lead, partly as result of its chemical constituents which has hepatoprotective properties.