Efficacy of Moringa oleifera Aqueous Extract in Inhibiting Tamoxifen®-Induced Physiological Hepatic Deterioration in Male Albino Rats.

A Essawy ,N Oulad Sayad ,M Beeker ,G Abdel-Wahhab ,Roya Ranjbar Saber

doi:10.21608/eajbsz.2017.13436

Abstract

Tamoxifen citrate (TAM) is a widely used drug in breast cancer treatment. It showed a degree of hepatic carcinogenesis. The purpose of this study was to elucidate the antioxidant capacity of Moringa oleifera aqueous extract (MAE) against TAM-induced liver injury. A model of liver injury in male rats was done by orally administration of TAM in a dose of 3mg/Kg/3days for consecutive six weeks to evaluate the drug-toxicity in combination with MAE in a dose of 300mg/Kg/day for similar period. The model of TAM-intoxication elicited significant elevation in serum alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT) activities as well as hepatic levels of the oxidative stress markers (MDA and NO),lipid profiles and inflammatory marker(TNF-α) associated with a significant depletion of anti-oxidative marker (GSH).The oral administration of MAE in combination with TAM-intoxicated rats, resulted in significant improvements in ALAT,ASAT and anti-oxidative marker (GSH)with significant decrements in MDA, NO and lipid profiles. The data obtained from this study speculated that MAE has the capacity to scavenge free radical and can protect against oxidative stress induced by TAM intoxication. Supplementation of MAE could be useful in alleviating tamoxifen-induced liver injury in rats.

Highlights

Since ancient times, plants remained major natural resource in the world (Kalia, 2005); and these plants have a great demand in both developed and developing countries (Yirga et al, 2011)
In accordance with the data obtained from this study, TMX administration resulted in decrease in the body weight gain
The loss in the body weight gain, from our point of view, may be due to the disturbance in the animals, appetite, digestive system physiology, food absorption and food assimilation as a consequence to Tamoxifen®, but animals' those were treated with Moringa oleifera aqueous extract (MAE) after TMX recorded a significant improvement in the body weight gain in compare to the Tamoxifen®intoxicated group

Summary

Introduction

Plants remained major natural resource in the world (Kalia, 2005); and these plants have a great demand in both developed and developing countries (Yirga et al, 2011). Even the modern pharmacopoeia still contains at least 25%of drugs derived from plants as it was suggest that over 9000 herbs have known medicinal applications among various cultures and countries (Kalia, 2005). Moringa oleifera Lam is a well-known widely distributed species of Moringaceae family enriched with many bioactive compounds such as kaempferol, rhamnetin, quercitin, chlorogenic acid, rutin, apigenin that exhibit antimicrobial, antiinflammatory, anti-cancer, anti-diabetic effects (Karthivashan et al, 2013; Ezuruike & Prieto, 2014, Anwar et al, 2007; Coppin et al, 2013) (Fig. 1). It has recently been evaluated for its hepatoprotective effects (Das et al, 2012; Sharifudin et al, 2012)

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Conclusion