Interactive technologies require the user to frequently generate purposeful movements, where actions are guided towards targets. While delays between the movement onset and the corresponding update within the virtual space have been shown to impair target acquisition, the relative contribution of the individual latency parameters remains unclear. This paper investigates how changes in latency, the moment-by-moment variability in the latency (jitter amplitude) and the rate of variability (jitter frequency), affect the speed and accuracy of target acquisition at different mean latencies. Experiment 1 explored changes in the mean latency and jitter amplitude. As the mean latency increased, we observed substantial impairments in completion time and accuracy, with increased variability in performance. There was also an interaction between the mean latency and jitter amplitude: although large jitter amplitudes caused a small completion time impairment, this effect decreased as the mean latency increased. Experiment 2 isolated the effect of the jitter by investigating changes in the jitter amplitude and jitter frequency at a fixed mean latency. Here, we observed completion time impairments from 67ms amplitude and accuracy impairments from 134ms amplitude. Like Experiment 1, the effect of the amplitude was small. Notably, we found no evidence that changes in the jitter frequency significantly influenced performance. Overall, increases in the mean latency contributed most to the impairment, disrupting acquisition speed and accuracy as it increased. Large jitter amplitudes also disrupted speed and accuracy, but this was a comparatively small effect that was mediated by the mean latency.