Three simple, accurate, economical and reproducible spectrophotometric methods have been developed for the simultaneous estimation of Gatifloxacin (GTX) and Ketorolac Tromethamine (KTA) in ophthalmic dosage form. The first method is Q analysis method based on absorbance ratio at two selected wavelengths 303 nm (iso-absorptive point) and 292 nm (λ max of Gatifloxacin). The second method is Simultaneous equation method based on measurement of absorbance at two wavelength 292 (λ max of Gatifloxacin) and 319 (λ max of Ketorolac Tromethamine) and the third method is Area under curve method and wavelength ranges selected were 290-294 nm (λ 1 -λ 2 ) for Gatifloxacin and 317-321 nm (λ 3 -λ 4 ) for Ketorolac Tromethamine. The linearity lies between 1-12 μg/ml for (GTX) and 1-12 μg/ml for KTA for all the three methods. The results of the analysis were validated statistically and recovery studies were carried out as per ICH guideline. As the % RSD was found less than 2, all the methods were proved to be precise and accurate and can be successfully applied for the simultaneous determination of both the drugs in bulk and ophthalmic dosage form.