Abstract
Three simple, accurate and economical methods have been developed for the estimation of norfloxacin and ornidazole in tablet dosage form. First method is based on the simultaneous equations, wavelengths selected for analysis were 273.0 nm (λmax of norfloxacin) and 318.5 nm (λmax of ornidazole), respectively, in 0.1N NaOH. Second method is Q-analysis method, based on absorbance ratio at two selected wavelengths 297.0 nm (iso-absorptive point) and 318.5 nm (λmax of ornidazole). Third method is first order derivative spectroscopy using 297.5 nm (zero cross for norfloxacin) and 264.0 nm (zero cross for ornidazole). The linearity was obtained in the concentration range of 4-20 μg/ml and 5-25 μg/ml for norfloxacin and ornidazole, respectively. The results of the analysis have been validated statistically and by recovery studies.
Highlights
Norfloxacin (NF), chemically 1-ethyl-6-fluoro-1,4dihydro-4-oxo-7-(1-piperazinyl)-3-quinoline carboxylic acid[1], is a synthetic broad spectrum fluoroquinolone antibacterial agent used in the treatment of urinary and genital tract infection
Norfloxacin is official in USP, BP and IP whereas Ornidazole is not official in any pharmacopoeia
Literature survey revealed that a number of methods have been reported for estimation of Norfloxacin[3,4,5,6] and Ornidazole[7,8,9,10,11,12] individually or in combination with other drugs
Summary
Norfloxacin (NF), chemically 1-ethyl-6-fluoro-1,4dihydro-4-oxo-7-(1-piperazinyl)-3-quinoline carboxylic acid[1], is a synthetic broad spectrum fluoroquinolone antibacterial agent used in the treatment of urinary and genital tract infection. There is no analytical method reported for the simultaneous estimation of Norfloxacin and Ornidazole in a combined dosage formulation. Procedure Method A: Simultaneous Equation Method For the selection of analytical wavelength, solutions of NF and OZ (20 μg/ml, each), were prepared separately by appropriate dilution of standard stock solution and scanned in the spectrum mode from 400 nm to 200 nm.
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