Simultaneous Spectrophotometric Methods for Estimation of Cefpodoxime Proxetil and Potassium Clavulanate in Tablet Dosage form

Abstract

Three UV spectrophotometric methods have been developed for the simultaneous estimation of Cefpodoxime proxetil (Cef) and Potassium clavulanate (Pot.clav.) in combined tablet dosage forms. The first method involves Q-absorbance ratio method, the sampling wavelengths selected are, 315.0nm (isoabsorptive point) and 233.0 nm (lmax of Cef). The second method is the First order derivative method, the sampling wavelengths selected are 269.0 nm and 240.0 nm for estimation of Cef and Pot.clav. respectively. In both the methods, the linearity range for both Cef and Pot.clav. was in the concentration range of 1.5-50 μg mL -1 . The third method based on ratio derivative spectrophotometry, involves measurement of absorbances at the amplitudes in the first order derivative of the ratio spectra at 246.0 nm and 327.0 nm for Cef and Pot.clav. respectively over the concentration range of 2.5-50 μg mL -1 for both the drugs. The results of the analysis were validated statistically and recovery studies carried out as per ICH guidelines. The developed methods are rapid, precise, accurate, rugged and can be employed for the routine estimation of Cefpodoxime proxetil and Potassium clavulanate in both bulk and tablet dosage form.