Abstract
Second order derivative spectroscopy method was developed and validated for the simultaneous estimation of Diclofenac sodium (DFS) and Serratiopeptidase (SPD) in bulk and tablet dosage form. Accurate and Precised UV Spectrophotometric method with good sensitivity has been developed for simultaneous estimation of DFS and SPD. The method employs Second order derivative based on the measurement of absorbance of DFS at ZCP 264.20 nm and SPD at ZCP 295.20 nm. The calibration curve was linear in a concentration range of 5-30 μg/ml for DFS and 25-150 μg/ml for SPD. The developed method was validated as per ICH guideline, for its accuracy, precision, LOD, LOQ and the results were found to be satisfactory, thus the method is specific, rapid and simple with good sensitivity for estimation of DFS and SPD in marketed dosage form. Keywords: Diclofenac sodium, Serratiopeptidase, Second order derivative method, Validation
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