Simultaneous Spectrophotometric Estimation of Hydrochlorothiazide, Atenolol and Losartan Potassium in Tablet Dosage Form

Abstract

Two UV spectrophotometric methods have been developed for the simultaneous estimation of Hydrochlorothiazide (Hctz), Atenolol (Atn) and Losartan Potassium (Los) in combined tablet dosage forms. The first method involves determination using the Area under curve, the sampling wavelength intervals selected are 274.5-270.5 nm, 226-222 nm and 252-248 nm over the concentration ranges of 0.5-30 mcg mL -1 , 1-50 mcg mL -1 and 1-60 mcg mL -1 for Hctz, Atn and Los respectively. The second method involves determination using the multicomponent mode of the UV visible spectrophotometer, the sampling wavelengths selected are 272.5 , 224 and 250 nm over the concentration ranges of 0.5-30 mcg mL -1 , 1-50 mcg mL -1 and 1-60 mcg mL -1 for Hctz, Atn and Los respectively. The results of the analysis were validated statistically and recovery studies were carried out as per ICH guidelines. The developed methods are simple, rapid, precise, accurate and can be employed for the routine estimation of Hydrochlorothiazide, Atenolol and Losartan Potassium in both bulk and tablet dosage form.