Traditional teaching methods have been placed under strain in recent years with the advent of new technologies and the ongoing COVID-19 pandemic. This has forced a migration towards virtual education, which has placed a greater emphasis on self-learning. Students are currently presented with two main options: the conventional lectures or the online videos on the topic. The former has the advantage of interaction between the students and the lecturer, whereas the latter has the advantage of presenting the subject in a dynamic style. We have created NMJLab, a didactical tool that combines both the interactive features of the traditional lecture with the ability of presenting the subject in a dynamical format, as well as a self-assessment component for the student to verify their mastery on the topic. We depicted the physiological cycle of muscle excitation and relaxation at the neuromuscular junction, along with four additional pathologies (myasthenia gravis, Lambert-Eaton myasthenic syndrome, tetanus, and botulism). Once the student has interacted with the simulation, they are presented with an assessment to gauge their understanding of the topic. At every stage, a Help option guides the student through the process. The software was implemented using LabVIEW 2022 from National Instruments, Inc. for both Mac and PC devices. It is part of the curriculum of the Integumentary and Musculoskeletal Systems course at California Northstate University College of Medicine. The reception by the students was positive, and similar modules of this interactive tool are planned to be incorporated into our basic science curriculum.