Taila Kalpana (Medicated oil) are integral part of Ayurvedic treatment and Taila (Oil) can be used for both Bahya (Externally) and Abhyantara Chikitsa (Internally). Standardization of Ayurvedic formulations is an important step for establishment of biological activity, consistent chemical profile, or quality assurance for production. The Apamarga Ksharasootra is well proven to be an effective treatment for fistula-in-ano and has been standardized by the CCRAS, an apex research organization of Government of India in the field of Indian system of medicine. Mechanical introduction of the Ksharasootra into the tract is complex and painful procedure. More over it has limitation in the treatment of multiple fistulae. Injection of the liquid into the tract can minimize many of these drawbacks as it can diffuse throughout the fistulous tract and it is simple, painless, non-invasive and cost effective management. So Apamargakshara Taila was prepared as mentioned in classics for the purpose to evaluate the effects in Bhagandara (Fistula-in-ano). Before clinical trial, standardization of Apamargakshara Taila was performed to know its physical, chemical and HPTLC profile as per WHO and AYSUH testing guidelines and it was found that, specific gravity was 0.9431, rancidity was slightly oxidised, viscosity was 89.0120, refractive index was 1.4699, acid value was 2.20, saponification value was 161.90, unsaponification matter was 3.47, iodine value was 82.43 and ester value was 159.70. At 254 nm and 366 nm showed 5 and 12 spots respectively and after derivatisation also showed 12 spots.