Abstract
Punica granatum is a shrub that has a bushy appearance, have a good n umber of varieties, cultivated either as commercial crop for the production of pomegranates or as an ornamental crop for its beautiful flo wers. Among the ornamental crop of Punica granatum, the “Double flower” producing plant gains much significant value as it is used in Unani drug un der the name of Gulnar for treating many ailments. The present study was aimed to investigate and compare the phytoconstitutents present in both the flowers using TLC and HP TLC methods. The normal and double flo wers of P. granatum were extracted with chloroform and alcohol separately using soxhlet apparatus. The TLC and HPTLC finger print profile of the extracts with marker compounds were studied using HPTLC Instrument, CAMAG (Switzerland). The TLC and HPTLC profile of chloroform and alcohol extract of normal and double flowers of P. granatum were exploited. The compound gallic acid and ethyl gallate, chromatographed together served as marker compound. The results revealed th e similar and different phytoconstituents of both the flowers. The TLC and HPTLC finger print chromatograms not only demonstrates the similarities and differences bet ween the flowers but also sho ws the presence of tested marker compounds gallic acid and ethyl gallate in alcohol extracts of both the flower types apart from acting as a quality control tool.
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