Simultaneous estimation of Guggulsterone E & Z and Tinosporaside in Jivitprada vati by HPTLC method

Abstract

Jivitprada vati is an Ayurvedic formulation, which is consists of Guggul, Shilajit and Galodhan Guggulsterone E & Z and Tinosporaside are active constituents that are used for rheumatoid arthritis and erectile dysfunction. The purpose of this study was to develop an HPTLC method of quantitative estimation of marker compounds, Guggulsterone E & Z and Tinosporaside in laboratory prepared authentic formulation and compare with three different marketed formulation. The marker compounds were isolated from plant material and authenticated by comparing its UV spectrum, IR spectrum and GC-MS fragmentation pattern with standard marker and its literature studied. The four formulations were subjected to methanol extractions by Soxhlet apparatus. Guggulsterone E & Z and Tinosporaside were quantified in the above extracts by HPTLC method. The detection and quantification was performed at a wavelength of 230 nm. The laboratory formulation was found to contain 2.18% of Guggulsterone E, 1.898% of Guggulsterone Z and 0.954% of Tinosporaside while in case of marketed formulation MF-1, MF-2 and MF-3 was found to contain, respectively, 1.06%, 0.527%, 0.318% of Guggulsterone E and 0.914%, 0.487%, 0.24% of Guggulsterone Z in the methanolic extracts of formulations, whereas in MF-1 and MF-2 was found very less amount of Tinosporaside (respectively, 0.347% & 0.14%) except in MF-3 which was devoid of Tinosporaside. The method was found to be linear, precise and accurate for quantitative estimation of E & Z Guggulsterone and Tinosporaside in different formulation. Key words: Guggulsterone E and Z, HPTLC, Jivitprada vati, laboratory formulation, Tinosporaside