Abstract
BackgroundMahasudarshan Churna (MC) is a polyherbal Ayurvedic medicine that is employed in fever (especially chronic type), cold and malaria, improvement of digestion and appetite, removes toxins from the blood, boosts immunity and protects against common bacterial infections.MethodsValidation and quantification of oleanolic acid (OA), ursolic acid (UA), mangiferin (M), gallic acid (GA), quercetin (Q) and curcumin (C) in commercial MC formulations by HPTLC method. Mobile phase, hexane: ethyl acetate: acetone (16.4: 3.6: 0.2, v/v) was used for the separation of OA and UA; ethyl acetate: glacial acetic acid: formic acid: water (20: 2.2: 2.2: 5.2 v/v) for the development of M; and toluene: ethyl acetate: formic acid (13.5: 9: 0.6 v/v) for the separation of GA, Q and C in crude sample extracts. Visualization and scanning were performed at λ = 530 nm for OA and UA, at λ = 254 nm for M and at λ = 366 nm for GA, Q and C. In addition, HPLC-PDA analysis was used to confirm the HPTLC results.ResultsMajor bio-active compounds in MC formulations were oleanolic acid (1.54–1.78%), mangiferin (1.38–1.52%) and gallic acid (1.01–1.15%); followed by ursolic acid (0.79–0.98%), curcumin (0.45–0.67%) and quercetin (0.22–0.34%).ConclusionAnalysis of bio-active compounds in the present study was performed using HPTLC methods and later HPTLC results were compared with HPLC. These two methods give comparable results and there was no statistically significant difference between the mean values for all extracts. Present study concluded that this HPTLC technique is low cost, fast, precise, and accurate which can be employed for the quantification of xanthonoid (M), triterpenoids (OA, UA) and phenolics (GA, Q and C) in samples/formulations. Furthermore, present HPTLC method can be conveniently employed for routine quality control analysis of all the six marker compounds in marketed Ayurvedic/herbal formulations.
Highlights
Mahasudarshan Churna (MC) is a polyherbal Ayurvedic medicine that is employed in fever, cold and malaria, improvement of digestion and appetite, removes toxins from the blood, boosts immunity and protects against common bacterial infections
The authentic standards - Mangiferin, Oleanolic acid, Ursolic acid, gallic acid, curcumin and quercetin standards were purchased from Sigma-Aldrich (USA)
Development of optimum mobile phase In this study, quantitative estimation of specific bioactive compounds viz. M, oleanolic acid (OA), ursolic acid (UA), gallic acid (GA), CA and curcumin was conducted in the commercial polyherbal formulations using High-performance ThinLayer Chromatography (HPTLC) technique
Summary
Mahasudarshan Churna (MC) is a polyherbal Ayurvedic medicine that is employed in fever (especially chronic type), cold and malaria, improvement of digestion and appetite, removes toxins from the blood, boosts immunity and protects against common bacterial infections. Ayurveda system of medicine is beneficial to mankind, but still there is a lack of proper standardizing techniques for the assessment of their quality, quantity and efficiency. According to WHO guidelines, chromatographic techniques are reliable and effectively implemented to standardize and quantify the major biomarker compounds from single drug and polyherbal formulation. The application of medicinal plants in curing diseases is the oldest prevailing system to deal with illness. 80% population depends upon traditional Medicine (TM) system to cure innumerable deadly diseases like cancer, AIDS, Malaria etc. Much attention and research has been attempted in current years, there is still insufficient information about the phytochemical and metabolomics of herbal medicines, which has created a major challenge in standardization methods [2]. Framework has been required for the quality control and standardization protocols of the herbal drugs
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