Phytochemical analysis, HPTLC finger printing, in vitro antioxidant and cytotoxic activity of Cymodocea serrulata

Abstract

ObjectiveTo investigate the phytochemical analysis, HPTLC finger printing, in vitro antioxidant and cytotoxic activity of the methanolic extract of Cymodocea serrulata (MECS). MethodsPhytochemical analysis was carried out to determine the presence of phenol, triterpenoids, flavones, alkaloids, reducing sugars, glycosides, saponins, quinones, proteins, tannins and anthraquinones. HPTLC finger printing was performed using Camag Linomat-V. Antioxidant potential of MECS was determined by DPPH radical scavenging assay, lipid peroxidation inhibition assay and ascorbic acid estimation. Cytotoxic activity was assessed using MTT assay on HeLa cells. ResultsPhytochemical analysis and HPTLC profile of MECS revealed that it possess various phytoconstituents. DPPH radical scavenging assay and lipid peroxidation inhibition assay were tested from 1.5 μg/ml to 1000 μg/ml. The results of these studies showed that at 1000 μg/ml DPPH assay showed 87.81 ± 0.65 inhibition and lipid peroxidation inhibition assay showed 37.15 ± 1.96 inhibition. In cytotoxicity assay, MECS produced maximum percentage of growth inhibition 40.47% at 100 μg/ml. ConclusionThe results of the present study indicated that MECS possess antioxidant and cytotoxic activity and could be used as a source of antioxidant in the food industry. However further detailed phytochemical, pharmacological and in vivo studies should be the next step in the identification of active constituents.