Glucose Levels In Type Research Articles

Evaluation of the Cookies Formulated with Costus igneus Plant Material for Antidiabetic Activity

Diabetes Mellitus is a fast-growing disorder in the current scenario commonly affecting all age groups especially elder groups. The high blood glucose can be kept in control with nutraceutical foods. The present study is aimed to formulate a nutritionally rich cookie with Costus igneus leaf extract and to determine the effect of cookie consumption on decreasing blood glucose levels in type 2 diabetic patients. Cookies made with Costus igneus leaf powder etc. were analyzed for proximate analysis and phytochemical analysis with standard procedures. A total of 30 Type-II diabetic patients were selected for the study whose demographic variables and blood glucose levels were measured on the first day and after 15 days, 30 days of cookies consumption. The proximate and physic-chemical analysis showed cookies contain high amount of secondary metabolites including antioxidant compounds. Among the 30 study subjects, the mean levels of FBS and PBS were decreased significantly in all the four groups from day 0 to day 30 which indicates that C.igneus cookies consumption had good effect in reducing the blood glucose levels. However the HbA1C has been decreased by one unit in all the four study groups. The cookies made with Costus igneus plant material have good antimicrobial, antioxidant and antidiabetic activities and can be used as a therapeutic and functional food source for the treatment of overweight, obesity and diabetes.

Effect of exercise on blood glucose levels in type 2 uncomplicated diabetes mellitus patients: A pilot comparative study from a tertiary care center of north India

Study Design: Comparative pilot study. Objective: Type 2 Diabetes Mellitus is a very common disease affecting Indian population and exercise is an effective method to control blood sugar levels. The aim of this study is to demonstrate the positive effect of exercise in the management of Type 2 Diabetes Mellitus. Materials and Methods: The present study was carried out in Physical Medicine and Rehabilitation department of Patna Medical College and Hospital on the role of exercise in patients of Type 2 Diabetes Mellitus. A total of 40 patients were recruited for the study and divided into two groups- study and control group; 20 patients in each group. Result: The study shows that exercising in Type 2 Diabetes Mellitus according to the designed protocol is safe, tolerable and effective, leading to decrease in blood glucose level. It was not surprising that reduction in blood glucose level was associated with a functional improvement and has been documented with the statistically significant differences blood glucose level on most of the scales. Conclusion: We can say that supervised exercising in Type 2 Diabetes Mellitus under the condition described in this study seems to be a safe and effective treatment, with no associated systemic repercussions or complications. Nonetheless, further study and clinical trials are needed to confirm the results observed. It may be an error to limit to exercising as a single treatment. It can be applicable as a carrier or a co- adjuvant of other established therapeutic methods and diet modifications.

Infrared measurements of glucose in peritoneal fluid with a tuneable quantum cascade laser.

Fast and accurate continuous glucose monitoring is needed in future systems for control of blood glucose levels in type 1 diabetes patients. Direct spectroscopic measurement of glucose in the peritoneal cavity is an attractive alternative to conventional electrochemical sensors placed subcutaneously. We demonstrate the feasibility of fast glucose measurements in peritoneal fluid using a fibre-coupled tuneable mid-infrared quantum cascade laser. Mid-infrared spectra (1200–925 cm−1) of peritoneal fluid samples from pigs with physiological glucose levels (32–426 mg/dL, or 1.8–23.7 mmol/L) were acquired with a tuneable quantum cascade laser employing both transmission and attenuated total reflection (ATR) spectroscopy. Using partial least-squares regression, glucose concentrations were predicted with mean absolute percentage errors (MAPEs) of 8.7% and 12.2% in the transmission and ATR configurations, respectively. These results show that highly accurate concentration predictions are possible with mid-infrared spectroscopy of peritoneal fluid, and represent a first step towards a miniaturised optical sensor for intraperitoneal continuous glucose monitoring.

O-GlcNAcylation Mediates Glucose-Induced Alterations in Endothelial Cell Phenotype in Human Diabetes Mellitus.

BackgroundPosttranslational protein modification with O‐linked N‐acetylglucosamine (O‐GlcNAc) is linked to high glucose levels in type 2 diabetes mellitus (T2DM) and may alter cellular function. We sought to elucidate the involvement of O‐GlcNAc modification in endothelial dysfunction in patients with T2DM.Methods and ResultsFreshly isolated endothelial cells obtained by J‐wire biopsy from a forearm vein of patients with T2DM (n=18) was compared with controls (n=10). Endothelial O‐GlcNAc levels were 1.8‐ford higher in T2DM patients than in nondiabetic controls (P=0.003). Higher endothelial O‐GlcNAc levels correlated with serum fasting blood glucose level (r=0.433, P=0.024) and hemoglobin A1c (r=0.418, P=0.042). In endothelial cells from patients with T2DM, normal glucose conditions (24 hours at 5 mmol/L) lowered O‐GlcNAc levels and restored insulin‐mediated activation of endothelial nitric oxide synthase, whereas high glucose conditions (30 mmol/L) maintained both O‐GlcNAc levels and impaired insulin action. Treatment of endothelial cells with Thiamet G, an O‐GlcNAcase inhibitor, increased O‐GlcNAc levels and blunted the improvement of insulin‐mediated endothelial nitric oxide synthase phosphorylation by glucose normalization.ConclusionsTaken together, our findings indicate a role for O‐GlcNAc modification in the dynamic, glucose‐induced impairment of endothelial nitric oxide synthase activation in endothelial cells from patients with T2DM. O‐GlcNAc protein modification may be a treatment target for vascular dysfunction in T2DM.

Effect of High Intensity Interval Training on Blood Glucose Levels in Type 2 Diabetes

Abstract Background: Patients with type 2 diabetes are at signifi-cantly increased risk for cardiovascular morbidity and mortality compared with age-matched healthy control subjects. Aim of Study: Was to determine the effect of high intensity interval training on blood glucose levels in type 2 diabetes. Subject and Methods: Forty diabetic patients of both sexes (12 males and 28 females). They were assigned into two groups equal in number. All patients were diagnosed clinically with established T2DM for more than 7-year duration. Their ages ranged from 45 to 55 years selected from outpatient clinic of Kasr El-Aini, Cairo University, Egypt. Study group with mean age (51.07±3.31) years. and Control group with mean age (51.36± 2.83 ) years. The study group included 20 patients (7 males and 13 females) who practiced high intensity interval training at intensity level of (85-90% of MHR), three times per week for 12 weeks in addition to their optimized oral hypoglycemic medications. Symptom limited exercise test was performed before and after training (12 weeks) to determine maximum heart rate. The control group included 20 patients (5 males 15 females) who only received their optimized oral hypoglycemic medications. Fasting and post prandial blood glucose were measured initially before starting the treatment and after the end of the sessions in both groups. Results: Post-treatment there were a significant improve-ment in both fating and two hours post prandial blood glucose. There were a significant decrease in fasting and two hours post prandial blood glucose. Conclusion: The high intensity interval training is an effective tool to decrease insulin resistance and hyperglycemia this effect is mediated, in part, by the decrease in fasting and two hours post prandial blood glucose in type 2 diabetic patients which consequently helps in the treatment type 2 diabetes.

V-ATPase blockade reduces renal gluconeogenesis and improves insulin secretion in type 2 diabetic rats.

Vacuolar H+-adenosine triphosphatase (V-ATPase) stimulates vesicular acidification that may activate cytoplasmic enzymes, hormone secretion and membrane recycling of transporters. We investigated the effect of blockade of V-ATPase by bafilomycin B1 on renal gluconeogenesis, mitochondrial enzymes, and insulin secretion in type 2 diabetic rats. Spontaneous type 2 diabetic Torii rats were treated with intraperitoneal injection of bafilomycin B1 for 1 week, and the kidneys were examined after 24 h of starvation in metabolic cages. The renal expression and activity of V-ATPase were increased in the brush border membrane of the proximal tubules in diabetic rats. The blockade of V-ATPase by bafilomycin B1 reduced renal V-ATPase activity and urinary ammonium in diabetic rats. Treatment with bafilomycin suppressed the enhanced renal gluconeogenesis enzymes and mitochondrial electron transport enzymes in type 2 diabetic rats and reduced the renal cytoplasmic glucose levels. The insulin index and pancreatic insulin granules were decreased in diabetic rats with increased V-ATPase expression in islet cells, and treatment with bafilomycin B1 reversed these changes and increased the insulin secretion index. Hepatosteatosis in type 2 diabetic rats was ameliorated by bafilomycin treatment. As a consequence, treatment with bafilomycin B1 significantly decreased the plasma glucose level after 24 h of starvation in diabetic rats. In conclusion, a V-ATPase inhibitor improved plasma glucose levels in type 2 diabetes by inhibiting renal mitochondrial gluconeogenesis and improving insulin secretion.

Sitagliptin Inhibits Extracellular Matrix Accumulation and Proliferation in Lung Fibroblasts.

BackgroundFibroblasts activation-induced fibrosis can cause idiopathic pulmonary fibrosis (IPF). Excessive activation of fibroblasts contributes to poor healing or severe visceral fibrosis and even organ dysfunction. Sitagliptin acts as a dipeptidyl peptidase 4 inhibitor to reduce glucose level in type 2 diabetes, but its role in fibrosis of lung fibroblasts is elusive. We investigated the mechanism of sitagliptin in TGF-β-activated lung fibroblasts and evaluated the efficacy of sitagliptin in extracellular matrix accumulation and fibroblasts proliferation.Material/MethodsBy in vitro lung fibroblasts culture, we assessed the expression of lung fibroblasts biomarker (α-SMA) and extracellular matrix (Col-1, Col-3, fibronectin) following TGF-β stimulation and treatment with sitagliptin. Mechanistically, the phosphorylation level of Smad-3 protein in cells was analyzed using Western blotting, and the apoptosis level was assessed by Western blotting and flow cytometry. The degree of proliferation was determined using immunofluorescence and scratch-healing assay.ResultsWe found that treatment with sitagliptin attenuates fibroblasts activation following TGF-β stimulation. Furthermore, the extracellular matrix was decreased by sitagliptin treatment by suppressing the phosphorylation level of Smad-3 protein. We found that sitagliptin does not affect apoptosis in fibroblasts, but it does affect the degree of proliferation of lung fibroblasts, thus ameliorating fibrosis after TGF-β stimulation.ConclusionsSitagliptin inhibits fibrosis in TGF-β-induced lung fibroblasts activation, which restrains extracellular matrix formation and cell proliferation in fibroblasts. Therefore, sitagliptin appears to have promise as a treatment of fibroproliferative disease caused by activation and proliferation of fibroblasts.

Prediction of type 2 diabetes mellitus with saliva- A pilot study

Aim: To validate the usage salivary glucose levels to monitor type 2 diabetes. Materials and Methods: 100 type 2 diabetes mellitus patients were included in the study. The fasting whole saliva and serum was collected and were analyzed for glucose by using autoanalyzer. The results were analyzed statistically. Results: A Significant correlation was obtained between salivary and serum glucose levels in type 2 diabetic patients and in control group. Conclusion: Salivary glucose levels can serve as a marker in diagnosing and monitoring type 2 diabetes mellitus. Keywords: Diabetes mellitus, Salivary glucose, Serum glucose.

The Effect of 12 Weeks Resistance Training on FOXO1 Expression in Hepatocytes, Glucose and Insulin in Diabetic Rats- A Brief-Report

Objective: In diabetic patients, hyperglycemia is associated with impaired FOXO signaling pathways in liver cells. This study aimed to determine the effect of resistance training on FOXO1 expression in liver hepatocytes and fasting glucose levels in type 2 diabetic rats. Materials and Methods: In this experimental study, type 2 diabetes induced by intraperitoneal injection of nicotinamide-STZ in 16 male wistar rats (220±20 g) and were randomly divided into exercise (n=8) and control (n=8) groups. Exercise group were participated in resistance training program (12 weeks, 5 days/weekly). Fasting glucose and insulin as well as FOXO1 expressioninliver hepatocytes were measured lasted exercise session of 2 groups and compared by independent T-test. Results: Compared to control group, resistance training resulted in significant decrease in fasting glucose ( P -value< 0.0001) and decrease in serum insulin ( P -value< 0.0001). However, no significant difference was found in FOXO1 expression in liver cells between exercise and control groups ( P -value: 0.725). Conclusion: Based on this data, improvement of glycemic profile in response to resistance training in diabetic rats cannot be attributed to FOXO1 expression in liver cells. This improvement may be attributed to an increase in serum insulin or other hormonal or genetic changes in response to resistance exercise, which will require further studies in this area.

Use of Graph Based Descriptors for Determination of Structural Features Causing Modulation of Fructose-1,6-Bisphosphatase.

Fructose-1,6-bisphosphatase performs a significant function in regulating the blood glucose level in type 2 diabetes by controlling the process gluconeogenesis. In this research work optimal descriptor (graph) based quantitative structural activity relationship studies of a set of 203 fructose-1,6-bisphosphatase has been performed with the help of Monte Carlo optimization. Distribution of compounds into different sets such as training set, invisible training set, calibration set and validation sets resulted in formation of splits. Statistical parameters obtained from quantitative structural activity relationship modeling were good for various designed splits. The statistical parameters such as R2 and Q2 for calibration and validation sets of best split developed were found to be 0.8338, 0.7908 & 0.7920 and 0.7036 respectively. Based on the results obtained for correlation weights, different structural attributes were described as promoters and demoters of the endpoint. Further these structural attributes were used in designing of new fructose-1,6-bisphosphatase inhibitors and molecular docking study was accomplished for the determination of interactions of designed molecules with the enzyme.

An increased focus on stress for the management of blood glucose levels in type 1 diabetes: a case report

An increased focus on stress for the management of blood glucose levels in type 1 diabetes: a case report

English

Oxidative stress resulting from chronic hyperglycemia in diabetes is due to high production of reactive oxygen species and/or a decrease in the antioxidant defense system activity. The study was designed to investigate the ameliorative effect of methanolic extract of Dennettia tripetala (DT) on oxidative stress and blood glucose level in type II diabetic male Wistar rats. Type II diabetes was induced by a single intraperitoneal injection of streptozotocin (40 mg/kg) after 2 weeks of 10% fructose diet. Twenty-five (25) rats were randomly divided into five groups, namely: 1 (normal control), 2 (negative control), 3 (10 mg/kg of glibenclamide), 4 (100 mg/kg of DT extract) and 5 (200 mg/kg of DT extract). The administration of the extract caused a significant decrease in blood glucose levels in all treatment groups. A significant (P<0.01) decrease in malonaldehyde (MDA) activity was observed in the group treated with 100 and 200 mg/kg of DT extract compared to diabetic control. Catalase (CAT) activity showed a significant (P<0.05) increase in the group treated with 200 mg/kg of DT extract compared to the normal control. However, the extract did not affect GSH-Px, SOD and CAT activities. The findings suggest ameliorative effect of DT extract in diabetic Wistar rats. Key words: Dennettia tripetala, diabetes mellitus, oxidative stress, lipid peroxidation, Wistar rats.

Prolonged melittin release from polyelectrolyte-based nanocomplexes decreases acute toxicity and improves blood glycemic control in a mouse model of type II diabetes

Gating modifier toxins (GMTs) from animal venom have shown great potential in controlling blood glucose levels in type II diabetes (T2D), but their high acute toxicity and quick clearance in the body hamper their potential therapeutic use. Inspired by their highly positive charge, we have developed a nanocomplex system based on polyelectrolytes, in which strong interactions form between positively charged GMTs and negatively charged dextran sulfate (DS). Using melittin as a model GMT and adapting flash nanocomplexation (FNC) technology for complex preparation, uniform nanocomplexes (polydispersity index: ~0.1) with high melittin encapsulation efficiency (~100%), high payload capacity (~30%), and tunable release profiles were formulated. In contrast to the high acute liver toxicity and low survival rate (60% after 8days) observed after a single intraperitoneal (i.p.) injection of 3mg/kg free melittin, melittin-loaded nanocomplexes displayed improved safety (100% survival after 8days) due to prolonged melittin release. In a mouse model of T2D, a single i.p. injection of nanocomplexes decreased the blood glucose level to 12mmol/L within 12h and maintained it within the therapeutic range (<15mmol/L) for 48h. In addition, body weight decreased following treatment. This GMT/DS binary system shows great promise due to its simple components, facile preparation method, and enhanced potential druggability, including a decreased dosing frequency, decreased acute toxicity, and improved pathological indicators.

Effect of Laparoscopic Mini Gastric Bypass and Laparoscopic Sleeve Gastrectomy on Blood Glucose Level in Type 2 Diabetes Mellitus Obese Patients; a Prospective Study

Background: Type 2 diabetes mellitus (T2DM) is now a global health problem. The World Health Organization estimates that between 2000 and 2030, the number of diabetic patients will increase by 114%. T2DM and morbid obesity are conditions representing increasing public health threats. They are associated with significant morbidity and mortality. Despite lifestyle modifications and medical support, glycemic control remains difficult to achieve in obese diabetic patients. Objective: To compare the effect of laparoscopic mini gastric bypass and laparoscopic sleeve gastrectomy in controlling blood glucose level in type 2 DM obese patients. Patients and methods: This prospective study was conducted on 40 adult morbid obese patients with T2DM. Twenty patient underwent laparoscopic mini-gastric bypass and the other 20 patients underwent sleeve gastrectomy. All patients were followed up at 1, 3, 6, 9 and 12 months post-operatively by measuring BMI, excess weight-loss (EWL), Fasting and 2 hours post prandial blood glucose level, HbA1C also postoperative gastrograffin study to exclude complications. Results: MGB was superior to SG in controlling T2DM along 1 year of follow up with lower 1-year HBA1C, FBG,2HPP blood glucose level. Conclusion: MGB is safe and effective in controlling T2DM in morbidly obese patients candidate for bariatric surgery.

Blood Glucose Prediction with Variance Estimation Using Recurrent Neural Networks

Many factors affect blood glucose levels in type 1 diabetics, several of which vary largely both in magnitude and delay of the effect. Modern rapid-acting insulins generally have a peak time after 60–90 min, while carbohydrate intake can affect blood glucose levels more rapidly for high glycemic index foods, or slower for other carbohydrate sources. It is important to have good estimates of the development of glucose levels in the near future both for diabetic patients managing their insulin distribution manually, as well as for closed-loop systems making decisions about the distribution. Modern continuous glucose monitoring systems provide excellent sources of data to train machine learning models to predict future glucose levels. In this paper, we present an approach for predicting blood glucose levels for diabetics up to 1 h into the future. The approach is based on recurrent neural networks trained in an end-to-end fashion, requiring nothing but the glucose level history for the patient. Our approach obtains results that are comparable to the state of the art on the Ohio T1DM dataset for blood glucose level prediction. In addition to predicting the future glucose value, our model provides an estimate of its certainty, helping users to interpret the predicted levels. This is realized by training the recurrent neural network to parameterize a univariate Gaussian distribution over the output. The approach needs no feature engineering or data preprocessing and is computationally inexpensive. We evaluate our method using the standard root-mean-squared error (RMSE) metric, along with a blood glucose-specific metric called the surveillance error grid (SEG). We further study the properties of the distribution that is learned by the model, using experiments that determine the nature of the certainty estimate that the model is able to capture.

Platelet Count and its Correlation with Blood Sugar Level in Type 2 Diabetes Mellitus Patients

Introduction&#x0D; Diabetes Mellitus (DM) is defined as a complex disease with chronic hyperglycemiaand is a global public health problem; the long-term sequelae of which are long-term macrovascular and microvascular complications. Studies show role of hematological indices especially platelet indices in contributing to the vascular injury in diabetic patients. Thus, the aim of this study was to determine platelet indices and their correlation with fasting blood glucose levelin type 2 DM patients in comparison with healthy controls.&#x0D; Method&#x0D; A retrospective study was conducted at DM Wayanad Institute of Medical Sciences, Kerala, India from Istof January 2018 to 31st of June. A total of 238 participants (119 cases and 119 healthy controls) were selected using systematic random sampling technique. Data is retrieved from Medical Records Department. Parameters are lab investigation values those are already done on patients who came to DM WIMS. FBS was estimated using (Cobasintegra 400 plus) automated clinical chemistry analyzer and hematological parameters using fully automated (Sysmex XT-1800i) analyzer. Independent sample t-test. The sample size required to study the correlation is 90 at 5% level of significance and 80% power assuming the population correlation to be. 3 (moderate correlation). The statistical analysis was done using SPSS 15.0 version. After checking for normality Pearson's or Spearman correlation analysis is carried out to study the correlation. A P-value, 0.05 was considered as statistically significant.&#x0D; Result&#x0D; There was no significant correlation between platelet indices, mean platelet volume, platelet distribution width in the diabetic patients.&#x0D; Conclusion&#x0D; Even though previous study showed statistically significant difference in platelet indices of diabetic patients compared to controls in our study there is no such statistical significance. Further studies should be done in large population.

Glucagon-like peptide-1 receptor agonist exendin-4 mitigates lipopolysaccharide-induced inflammatory responses in RAW264.7 macrophages

Macrophages play a critical role in the immune response against pathogen invasion and injury. However, under pathological stress, macrophages could have aberrant roles and contribute to the pathogenesis of inflammatory associated diseases. Exenatide is a glucagon-like peptide 1(GLP-1) agonist, which belongs to the family of synthetic exendin-based incretin mimetic. Exendin related compounds reduce glucose levels in type 2 diabetes patients. The purpose of this study was to examine the anti-inflammatory effects of exendin-4 in LPS-induced activation of macrophages. We show that exendin-4 inhibits LPS-induced expression of inflammatory mediators (iNOS, COX-2, PGE2 and NO) and pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) in RAW264.7 macrophages. Exendin-4 pretreatment mitigates LPS induced cellular ROS production. Mechanistically, Exendin-4 suppresses the LPS-induced activation of the JNK and AP-1 pathway. Furthermore, exendin-4 suppresses both nuclear p65 accumulation and transfected NF-κB promoter activity, indicating it inhibits the activation of the NF-κB pathway. Our study demonstrates that the GLP-1 agonist exendin-4 has a potent anti-inflammatory effect independent on its glucose reducing ability, and exendin-4 has the potential implication to treat inflammatory associated diseases.

Indeks Glikemik Bahan Makanan Dengan Kadar Glukosa Darah Pada Penderita Diabetes Mellitus Tipe 2 Di Puskesmas Ranotana Weru

The food glycemic index is a scale or number of foodstuffs that if consumed can have an impact on blood glucose levels so it can be used as a way to control blood glucose levels. The purpose of this study to determine the relationship of glycemic index of food with blood glucose level at diabetes mellitus type 2 in working area of Ranotana Weru Health Center. This research is an observational research using cross sectional research design with sample consist of 34 respondents who fill up the criteria of inclusion and exclusion. Data retrieval is done through interviews using food frequency questionnaire form (FFQ) and blood glucose levels obtained from the examination using autocek. Univariate analysis is done by frequency distribution and bivariate analysis using Fisher’s Exact Test. The results showed that of 34 respondents most of the respondents have blood glucose levels when the uncontrolled ≥180 mg/dl and consumed a high food glycemic indexs of 28 respondents (82,3%). While respondents have controled blood glucose levels of &lt;180 mg/dl and consumed a low food glycemic index is 5 respondents (14,7%). Average blood glucose levels of respondents is 237,74% mg/dl. The statistical test is done obtained that there was correlation of food glycemic index with blood glucose level with value p = 0,000 (p &lt;0,05). Conclusion, there is correlation of food glycemic index with blood glucose level in type 2 diabetes mellitus in working area of Ranotana Weru Health Center.

Pengaruh Pemberian Minuman Fungsional Terhadap Penurunan Kadar Glukosadarah Puasa Pada Penderita Diabetes Melitus

Indonesia is currently the fourth country with the largest number of DM patients in the world. Functional food is beneficial for the health of DM patients, for example bananas and soybeans. Kepok bananas contain dietary fiber inulin and antioxidants (β carotene). Soybeans contain isoflavones, fiber, and proteins in the form of amino acids arginine and glycine. This study aims to look at the effect of giving functional drinks a mixture of soybean and banana milk on decreasing fasting blood glucose levels in type II DM patients. Type of Quasy-experimental research with pre test and post test with control group design. Subjects were taken using the Simple Random Sampling method and the subjects involved until the end of the study were 26 people. The intervention was carried out for 14 days and given as much as 330 ml. The equality of the subject characteristics of the two groups was analyzed using the chy squere test, and the effect of giving intervention materials on decreasing blood glucose levels was tested by independent t test. The results of the study were characteristics (84.6%) aged 50-60 years, (53.8%) female gender, (57.1%) having a family history of disease, 58.4% using glibenclamide anti-hyperglycemic drugs and BMI average in the risk category. . Result Δ It is known that there is an effect of giving functional drinks on decreasing fasting blood glucose levels where the average change is 11.5 mg / dl (p = 0.03).

Activity And Kinetics of α-Glucosidase Inhibition by Collagen Hydrolysate From Thunnus albacares Bone

Controlling of postprandial hyperglycemia using α-glucosidase inhibitors is one of the therapeutic approaches for the treatment of type 2 diabetes. Collagen hydrolysate, as an α-glucosidase inhibitor has not been reported. Therefore, This study intends to evaluate the activity and kinetics of inhibition of collagen hydrolysate against the α-glucosidase enzyme. Collagen hydrolysate was prepared from Thunnus albacares bone enzymatically using bacterial collagenase at hydrolysis times (0; 0.5; 1; 2; 3; 4; 5 and 6 h). The type of inhibition was determined by enzyme kinetics analysis. Collagen hydrolysate obtained at hydrolysis time of 1 h has the highest inhibitory activity of 24.47 %. Based on enzyme kinetics analysis, collagen hydrolysate showed a show a type of competitive inhibition. These results revealed that collagen hydrolysate has the potential as a natural antidiabetic in controlling blood glucose levels in type 2 diabetes.