Formulation Strategies Research Articles

Evolution of the structure of lipid nanoparticles for nucleic acid delivery: From in situ studies of formulation to colloidal stability

The development of lipid nanoparticle (LNP) based therapeutics for delivery of RNA has triggered the advance of new strategies for formulation, such as high throughput microfluidics for precise mixing of components into well-defined particles. In this study, we have characterised the structure of LNPs throughout the formulation process using in situ small angle x-ray scattering in the microfluidic chip, then by sampling in the subsequent dialysis process. The final formulation was investigated with small angle x-ray (SAXS) and neutron (SANS) scattering, dynamic light scattering (DLS) and cryo-TEM. The effect on structure was investigated for LNPs with a benchmark lipid composition and containing different cargos: calf thymus DNA (DNA) and two model mRNAs, polyadenylic acid (polyA) and polyuridylic acid (polyU). The LNP structure evolved during mixing in the microfluidic channel, however was only fully developed during the dialysis. The colloidal stability of the final formulation was affected by the type of incorporated nucleic acids (NAs) and decreased with the degree of base-pairing, as polyU induced extensive particle aggregation. The main NA LNP peak in the SAXS data for the final formulation were similar, with the repeat distance increasing from polyU<polyA<DNA, following the expected extent of base-pairing.

Electrospun nanofiber drug delivery systems and recent applications: An overview

Fiber technology plays a significant role in advancing drug research. While developing drug delivery systems, nanofiber technology has gained importance due to its high surface area to volume ratio and porosity. The most preferred nanofiber production method is the electrospinning method. This review focuses on electrospun nanofiber drug delivery systems, including analysis of critical process parameters affecting electrospinning and nanofiber properties. Moreover, the design strategies for nanofiber drug delivery systems are explored, including equipment preferences and design types. Given to these design strategies, it is possible to design either nanofiber mat or nanofiber itself. It has been shown that a tailor-made drug delivery system can be prepared using axial-based nozzles and other equipment options. In addition, the prominent features and usage areas of frequently used polymers are mentioned. General characterization studies of post-production nanofibers are discussed. Examples of areas of use for pharmaceutical purposes are presented. Finally, drug release kinetics were evaluated on electrospun nanofiber drug delivery systems. As a result of exhaustive evaluations, various effective applications in the literature have been shown to achieve remarkable results such as drug release manipulation, drug targeting, self-assembling formulation strategies. Therefore, it is clear that in the field of electrospun nanofiber technology, it is possible to modify all the process options and develop a unique drug delivery product, thanks to a comprehensive evaluation of the critical quality parameters of the drug product.

Strategic Planning for Development of Micro Enterprises at the Department of Trade, Industry, Cooperatives, and Small and Medium Enterprises, Soppeng Regency

MSMEs are businesses with a micro, small to medium production scale that can be owned by individuals or in the form of business entities of a certain type. In Indonesia, MSMEs are currently being worked on and continue to grow from year to year. This study aims to examine the strategic planning of micro-enterprise development in the Department of Trade, Industry, Cooperatives and Small and Medium Enterprises, Soppeng Regency. This type of research is descriptive qualitative. Data collection techniques were carried out by observation, interviews, and documentation. The data analysis technique used consists of data reduction, data display, and conclusion drawing. The results of the study show that Environmental Scanning (Environmental Analysis), in Soppeng Regency has abundant Natural Resources (SDA) which can be utilized by micro-entrepreneurs as a source of income by processing them into products that have sales value. Strategy formulation (Strategy Formulation), a strategic plan made by the PPK and UKM Office in accordance with the 2nd mission of the Regional Head, namely strengthening the realization of community welfare. Pay more attention to micro business actors so that they can prosper from the business they are involved in and develop. Strategy implementation (Implementation Strategy), the implementation of programs is inseparable from what has been stated in the strategic plan (renstra), but in implementing the programs researchers value it is still not optimal because of the lack of awareness of micro-entrepreneurs to register their business as fostered by the PPK Service and UKM. Evaluation and control (evaluation and supervision), evaluation of activities is carried out quarterly but for evaluation and supervision of micro business actors directly to the field the schedule is uncertain, this is due to the lack of funds to go directly to the field. Factors supporting the success of strategic planning are supported by the awareness of micro-entrepreneurs to register their businesses and complete all forms of licensing according to the type of business. The inhibiting factors for miscommunication between micro-entrepreneurs and the PPK and UKM Offices are that micro-business actors sometimes delay registering their businesses because they think that the management will be complicated and convoluted.&#x0D; Keywords: strategic planning, micro enterprises, department of trade, industry, cooperatives and small and medium enterprises

Emulgel Towards Novel Formulation Development: A Comprehensive Review

Emulgels are an innovative topical drug delivery system that combines the properties of emulsions and gels to enhance the delivery and efficacy of pharmaceuticals. These systems leverage the advantages of both components: the solubilizing capabilities of emulsions and the stable, bioadhesive properties of gels. This review aims to summarize the development, formulation strategies, evaluation techniques, and therapeutic applications of emulgels. A comprehensive literature search was conducted across various scientific databases including PubMed, Scopus, and Web of Science. Studies focusing on the formulation, characterization, and clinical efficacy of emulgels were included. The review evaluates the methodologies used in the preparation of emulgels, including the choice of gelling agents, emulsifiers, and active pharmaceutical ingredients (APIs). Analytical techniques for assessing the physicochemical properties and stability of emulgels were also examined. The review identified key formulation parameters that influence the performance of emulgels, such as the type and concentration of gelling agents (e.g., carbomers, xanthan gum), emulsifiers (e.g., Tween, Span), and oils. Optimization of these components significantly affects the viscosity, spreadability, and drug release profile of emulgels. Studies demonstrated that emulgels exhibit improved drug penetration and sustained release compared to conventional topical formulations. Therapeutic applications of emulgels encompass a wide range of conditions including dermatological disorders, pain management, and microbial infections. The review also highlights novel advancements such as the incorporation of nanoparticles and the development of stimuli-responsive emulgels. Emulgels represent a promising platform for topical drug delivery, offering enhanced stability, bioavailability, and patient compliance. The versatility in their formulation allows for the incorporation of diverse APIs, making them suitable for various therapeutic areas. Future research should focus on the clinical translation of emulgels, exploring their potential in personalized medicine, and addressing regulatory challenges. Emulgels have the potential to revolutionize topical therapy and provide new avenues for effective and targeted drug delivery.

A Comprehensive Study on Formulation of Herbal Sunscreen Using Aloe barbadensis, Cucumis sativus and Solanum lycopersicum

Sunscreen formulations play a crucial role in protecting the skin from the harmful effects of ultraviolet (UV) radiation. Recent advancements in nature and herbal ingredients have led to a surge of interest in formulation effective, eco-friendly sunscreens. This review provides a comprehensive analysis of herbal sunscreen formulations, specially focusing on Aloe barbadensis miller (Aloe vera), Cucumis sativus (Cucumber), and Solanum lycopersicum (Tomato) as key ingredients. Aloe vera, known for its soothing and hydrating properties, offers potential benefits enhancing the skin’s resilience against UV damage. Cucumis sativus contributes cooling and antioxidant effects, while Solanum lycopersicum provides substantial levels of lycopene, which is recognized for its photoprotective qualities. The review systematically examines the phytochemical profiles, extraction methods, and efficacy of these ingredients in sunscreen formulations. It also explores their synergistic effects when combined and evaluates their overall performance in terms of sun protection factor (SPF), stability, and skin compatibility. By synthesizing current research findings, this review aims to highlight the potential of these herbal components in developing effective, natural sunscreens and to provide insights for future research and formulation strategies in the field of dermatological protection.

Effects of Dietary Acidifiers and Low Acid-Binding Capacity-4 (ABC-4) Formulation Strategies on Nursery Pig Performance and Fecal Dry Matter

Effects of Dietary Acidifiers and Low Acid-Binding Capacity-4 (ABC-4) Formulation Strategies on Nursery Pig Performance and Fecal Dry Matter

Intraoral administration of probiotics and postbiotics: An overview of microorganisms and formulation strategies

Intraoral administration of probiotics and postbiotics: An overview of microorganisms and formulation strategies

Unlocking the Therapeutic Applicability of LNP-mRNA: Chemistry, Formulation, and Clinical Strategies.

Messenger RNA (mRNA) has emerged as an innovative therapeutic modality, offering promising avenues for the prevention and treatment of a variety of diseases. The tremendous success of mRNA vaccines in effectively combatting coronavirus disease 2019 (COVID-19) evidences the unlimited medical and therapeutic potential of mRNA technology. Overcoming challenges related to mRNA stability, immunogenicity, and precision targeting has been made possible by recent advancements in lipid nanoparticles (LNPs). This review summarizes state-of-the-art LNP-mRNA-based therapeutics, including their structure, material compositions, design guidelines, and screening principles. Additionally, we highlight current preclinical and clinical trends in LNP-mRNA therapeutics in a broad range of treatments in ophthalmological conditions, cancer immunotherapy, gene editing, and rare-disease medicine. Particular attention is given to the translation and evolution of LNP-mRNA vaccines into a broader spectrum of therapeutics. We explore concerns in the aspects of inadequate extrahepatic targeting efficacy, elevated doses, safety concerns, and challenges of large-scale production procedures. This discussion may offer insights and perspectives on near- and long-term clinical development prospects for LNP-mRNA therapeutics.

Load Restoration in Islanded Microgrids: Formulation and Solution Strategies

Load Restoration in Islanded Microgrids: Formulation and Solution Strategies

Optimal velocity planning based on the solution of the Euler-Lagrange equations with a neural network based velocity regression

Trajectory optimization is a complex process that includes an infinite number of possibilities and combinations. This work focuses on a particular aspect of the trajectory optimization, related to the optimization of a velocity along a predefined path, with the aim of minimizing power consumption. To tackle the problem, a functional formulation and minimization strategy is developed, by means of the Euler-Lagrange equation. The minimization is later performed using a neural network approach. The strategy is deemed Lagrange-Net, as it is based on the minimization of the energy functional, by the means of Lagrange's equation and neural network approximations.

FIELDS APART: EXPLORING THE IMPACT OF LAND FRAGMENTATION ON TECHNICAL EFFICIENCY IN MAIZE FARMING WITHIN GHANA'S TRANSITIONAL ZONE

This study investigates the intricate relationship between land fragmentation and technical efficiency in maize farming within the Transitional Zone of Ghana. Employing a comprehensive analysis, we examine how the division of land affects the operational efficiency of maize farmers in this region. Utilizing a combination of quantitative data and empirical evidence, our research sheds light on the nuanced dynamics and implications of land fragmentation on agricultural productivity. The findings contribute valuable insights to agricultural policy formulation and resource management strategies in similar agro-ecological contexts.

Key Features in Designing an Integrated Recall System for Dispatch in Mass Casualty Incidents; a Systematic Review.

Following Mass Casualty Incidents (MCIs), the sudden surge in demand for essential services disrupts the balance between available and required resources. This study aimed to systematically identify and categorize existing systems employed for dispatching professional or lay rescuers during emergencies. Adhering to the PRISMA 2020 Checklist, the research scrutinized international databases (PubMed, Scopus, and Web of Science) using formulated search strategies. Additionally, a manual search was conducted on Google Scholar and prominent journals employing specific keywords. Original articles introducing systems for dispatching rescuers to incident sites were included. Thirty-one of the 23051 initially identified documents were included for data extraction and quality assessment. The comprehensive analysis revealed twenty-two dispatch systems worldwide, contributing to life-saving efforts in emergencies. Additionally, an evaluation of the articles' quality using the Mixed Methods Appraisal Tool (MMAT) with five scores, indicated that more than two-thirds of the identified articles scored four or higher. Summarizing the data extracted from these systems, four distinct categories of recall system characteristics were identified: general, dispatcher, responder, and other features. Technology has the potential to revolutionize the delivery of healthcare services. This study highlights four key elements necessary for the development of dispatch systems that can effectively mobilize healthcare providers to the incident scene. These elements include general characteristics, dispatcher roles, responder requirements, and additional features, which equip researchers with the knowledge for designing effective systems to recall healthcare providers during MCI.

Comparative Analysis of Educational Policy Formulation and Implementation Strategies in Developed and Developing Countries.

Educational policy formulation and implementation are critical components for improving educational systems worldwide, yet significant disparities exist between developed and developing countries. This paper explores these disparities and presents a comparative analysis of the strategies employed in both contexts. Drawing on key theories like the Policy Cycle and Actor-Network Theory, the comparative analysis examined how these frameworks inform the processes of policy formulation and implementation. Data were drawn from 53 peer reviewed articles gotten through a systematic search in online databases including Google Scholar, Sematic Scholars, Paperity and Ethos. The analysis reveals that both developed and developing countries emphasize evidence-based policy making, stakeholder engagement, capacity building, and the integration of technology in education. Developed countries have a more robust financial resources, advanced technology, and stable political environments, enabling robust funding systems and continuous policy adaptation. In contrast, developing countries face challenges such as inadequate funding, political instability, and bureaucratic inefficiencies, focusing more on community engagement and infrastructure development. Despite these challenges, developing countries like Nigeria, Ghana, and Kenya have made significant strides in improving educational outcomes through strategic partnerships and targeted policy interventions. The study concludes with implications for practice and recommendations for policymakers in developing countries, emphasizing the need for diversified funding models, technological integration, strengthened governance, stakeholder engagement, and continuous evaluation to enhance educational policy effectiveness and sustainability. The findings highlight the importance of context-specific approaches and the potential for developing countries to learn from the experiences of developed nations in creating robust educational systems.

Formulation strategy to reach a balance among dietary essential amino acids for Nile tilapia juveniles

Formulation strategy to reach a balance among dietary essential amino acids for Nile tilapia juveniles

Synthetic mucus barrier arrays as a nanoparticle formulation screening platform.

A mucus gel layer lines the luminal surface of tissues throughout the body to protect them from infectious agents and particulates. As a result, nanoparticle drug delivery systems delivered to these sites may become trapped in mucus and subsequently cleared before they can reach target cells. As such, optimizing the properties of nanoparticle delivery vehicles, such as their surface chemistry and size, is essential to improving their penetration through the mucus barrier. In previous work, we developed a mucin-based hydrogel that has viscoelastic properties like that of native mucus which can be further tailored to mimic specific mucosal tissues and disease states. Using this biomimetic hydrogel system, a 3D-printed array containing synthetic mucus barriers was created that is compatible with a 96-well plate enabling its use as a high-throughput screening platform for nanoparticle drug delivery applications. To validate this system, we evaluated several established design parameters to determine their impact on nanoparticle penetration through synthetic mucus barriers. Consistent with the literature, we found nanoparticles of smaller size and coated with a protective PEG layer more efficiently penetrated through synthetic mucus barriers. In addition, we evaluated a mucolytic (tris(2-carboxyethyl) phosphine, TCEP) for use as a permeation enhancer for mucosal drug delivery. In comparison to N-acetyl cysteine (NAC), we found TCEP significantly improved nanoparticle penetration through a disease-like synthetic mucus barrier. Overall, our results establish a new high-throughput screening approach using synthetic mucus barrier arrays to identify promising nanoparticle formulation strategies for drug delivery to mucosal tissues.

Aging iridium oxide catalyst inks: a formulation strategy to enhance ink processability for polymer electrolyte membrane water electrolyzers.

Iridium oxide (IrO2) is recognized as a state-of-art catalyst for anodes of low-temperature polymer-electrolyte membrane water electrolyzers (PEMWE), one of the promising clean energy technologies to produce hydrogen, a critical energy carrier for decarbonization. However, typical IrO2 ink formulations are challenging to process in liquid-film coating processes because of their poor stability against gravitational settling and low viscosities. Here we report on time evolution of the microstructure of concentrated IrO2 inks in a water-rich dispersion medium, probed using a combination of rheology and X-ray scattering for up to four days. The inks progressively evolve from a predominantly liquid-like to a gel-like material with increasing aging time that can be leveraged as a formulation strategy to enhance their stability against sedimentation, and processability during electrode fabrication. We also elucidate the aging behavior by investigating the effects of ink formulation composition - ionomer concentration and solvent composition - and using the extended-DLVO theory. The implications of aging on electrode fabrication, including via direct coating onto membranes and porous transport layers, and membrane-electrode-assembly performance has also been examined. Our findings offer not only a facile but also an environmentally benign formulation strategy to enhance ink processibility, expand practical fabrication approaches, and advance PEMWE manufacturing.

A comparative impact chain analysis of 1999 Kocaeli and 2023 Kahramanmaraş earthquakes

Resilience in the face of crises is crucial for minimizing the impact of disasters and enabling rapid recovery. This study delves into the interlinked consequences of two seismic events that significantly impacted Türkiye in 1999 and 2023. Using an impact chain analysis, the aim is to provide a thorough understanding of the extensive effects on structures, infrastructure, and socio-economic dynamics. The research also examines the evolution of disaster management practices from the 1999 Kocaeli Earthquake to the more recent seismic events in 2023, highlighting advancements in risk management and resilience. Structurally, both seismic events revealed vulnerabilities in building design, emphasizing seismic shortcomings that led to widespread damage. Earthquakes exert a profound impact on critical infrastructure, affecting transportation, communication, and energy systems, with cascading effects that extend to the broader socio-economic landscape. The effectiveness of the methodology, particularly, the Impact Chain analysis, is emphasized as it reveals complex causal relationships. Visual representations support effective communication and collaboration among stakeholders, offering a holistic perspective on systemic risks. In conclusion, this study contributes to understanding disaster resilience and provides a foundation for subsequent research, policy formulation, and pragmatic strategies for disaster preparedness and response.

Exploration of Solubilization Strategies: Enhancing Bioavailability for Low Solubility Drugs

This review explores various strategies aimed at improving the solubilization of low-solubility drugs, including formulation design, nanoparticle technologies, prodrug strategies, and particle size reduction methods. Water solubility plays a crucial role in shaping bioavailability, formulation strategies, and therapeutic efficacy. Nanotechnology, particularly in nanomedicines, is a promising avenue to tackle solubility challenges, but faces barriers like production costs, formulation reproducibility, and varying pharmacokinetics. Despite these challenges, the burgeoning landscape of innovative drug delivery technologies offers advantages, particularly for formulation scientists. Understanding molecular properties is crucial for resolving these challenges, with solid dispersions and lipid-based delivery techniques emerging as sought-after solutions. Commercializing these advancements requires a leap in technology and infrastructure, making it essential to streamline the process and identify optimal approaches. Pioneering methodologies, such as Fagerholm's predictive model for human oral bioavailability based on chemical structure, demonstrate promising predictive accuracy. The integration of artificial intelligence and innovative solubility enhancement technologies is pivotal in transforming drug delivery, tackling solubility concerns, and streamlining research and development expenses.

Peran Pendidikan Agama Islam dalam Membangun Moralitas Siswa di Era Digital (Studi di SMP IT Mutiara Aulia Sei Mencirim)

This study aims to examine the role of Islamic religious education in building student morality in the digital era, focusing on a case study at SMP IT Mutiara Aulia Sei Mencirim. The digital age has a significant impact on student behavior, especially in the context of morality. This study aims to determine the role of Islamic religious education in building student morality in the digital era. The research method used is qualitative with a case study approach. The subjects of the study were selected by purposive sampling. Data was collected through observation, interviews, and document studies at SMP IT Mutiara Aulia Sei Mencirim. Data analysis is carried out with a qualitative data analysis approach, namely data reduction, data presentation, and conclusions. The results of data analysis show that Islamic religious education at SMP IT Mutiara Aulia Sei Mencirim has an important role in guiding students to face this challenge. Islamic teachers act as facilitators of moral learning who integrate Islamic religious values in students' daily lives. The research formulated several strategies that can be applied by SMP IT Mutiara Aulia Sei Mencirim to strengthen the role of Islamic religious education in the digital era, including responsive curriculum development, Islamic teacher training, and integration of religious values in school activities.

Quality by Design in Pharmaceuticals: A Review of its Impact on Regulatory Compliance and Product Quality.

The pharmaceutical industry has embraced the quality-by-design (QbD) approach as a promising development, formulation and manufacturing strategy. QbD provides a systematic and science-based framework for designing and producing high-quality products, with a particular focus on identifying, assessing and controlling risks throughout the development process. This review aims to assess the benefits of implementing QbD in pharmaceutical processes, evaluate its impact on regulatory compliance and explore its potential to enhance drug product quality. The primary objective of this review is to evaluate the influence of QbD on pharmaceutical development and manufacturing processes. It also seeks to examine the regulatory requirements associated with the implementation of QbD and highlight the advantages of this approach in terms of product quality and cost-effectiveness. Additionally, the review aims to explore the potential of QbD in improving the safety and efficacy of drug products. The QbD approach holds tremendous potential to revolutionize the pharmaceutical industry by optimizing drug development & manufacturing processes, reducing costs and enhancing product quality and consistency. However, implementing QbD requires a comprehensive understanding of the underlying science, as well as strict adherence to regulatory requirements in drug development and manufacturing. In conclusion, by embracing the QbD approach, the pharmaceutical industry can ensure the production of safe, effective and regulation-compliant products while simultaneously improving process efficiency. This strategic shift toward QbD represents a pivotal step in advancing pharmaceutical research and manufacturing capabilities, ultimately benefiting both the industry and more importantly, patients worldwide.