Floor Plate Research Articles

Dynamics of morphogen source formation in a growing tissue.

A tight regulation of morphogen production is key for morphogen gradient formation and thereby for reproducible and organised organ development. Although many genetic interactions involved in the establishment of morphogen production domains are known, the biophysical mechanisms of morphogen source formation are poorly understood. Here we addressed this by focusing on the morphogen Sonic hedgehog (Shh) in the vertebrate neural tube. Shh is produced by the adjacently located notochord and by the floor plate of the neural tube. Using a data-constrained computational screen, we identified different possible mechanisms by which floor plate formation can occur, only one of which is consistent with experimental data. In this mechanism, the floor plate is established rapidly in response to Shh from the notochord and the dynamics of regulatory interactions within the neural tube. In this process, uniform activators and Shh-dependent repressors are key for establishing the floor plate size. Subsequently, the floor plate becomes insensitive to Shh and increases in size due to tissue growth, leading to scaling of the floor plate with neural tube size. In turn, this results in scaling of the Shh amplitude with tissue growth. Thus, this mechanism ensures a separation of time scales in floor plate formation, so that the floor plate domain becomes growth-dependent after an initial rapid establishment phase. Our study raises the possibility that the time scale separation between specification and growth might be a common strategy for scaling the morphogen gradient amplitude in growing organs. The model that we developed provides a new opportunity for quantitative studies of morphogen source formation in growing tissues.

Lineage tracing of Shh+ floor plate cells and dynamics of dorsal-ventral gene expression in the regenerating axolotl spinal cord.

Both development and regeneration depend on signaling centers, which are sources of locally secreted tissue-patterning molecules. As many signaling centers are decommissioned before the end of embryogenesis, a fundamental question is how signaling centers can be re-induced later in life to promote regeneration after injury. Here, we use the axolotl salamander model (Ambystoma mexicanum) to address how the floor plate is assembled for spinal cord regeneration. The floor plate is an archetypal vertebrate signaling center that secretes Shh ligand and patterns neural progenitor cells during embryogenesis. Unlike mammals, axolotls continue to express floor plate genes (including Shh) and downstream dorsal-ventral patterning genes in their spinal cord throughout life, including at steady state. The parsimonious hypothesis that Shh+ cells give rise to functional floor plate cells for regeneration had not been tested. Using HCR in situ hybridization and mathematical modeling, we first quantified the behaviors of dorsal-ventral spinal cord domains, identifying significant increases in gene expression level and floor plate size during regeneration. Next, we established a transgenic axolotl to specifically label and fate map Shh+ cells in vivo. We found that labeled Shh+ cells gave rise to regeneration floor plate, and not to other neural progenitor domains, after tail amputation. Thus, despite changes in domain size and downstream patterning gene expression, Shh+ cells retain their floor plate identity during regeneration, acting as a stable cellular source for this regeneration signaling center in the axolotl spinal cord.

Metode Pelaksanaan Pekerjaan Struktur Balok dan Pelat Lantai 2 Pembangunan Gelanggang Generasi Muda Majalengka

Beams and Floor Plates are a very important construction structure in a building. In carrying out work on floor slabs and beams, a method is needed to complete the work in the field. Especially when facing obstacles caused by field conditions that do not match previous expectations. For this reason, applying construction implementation methods that are appropriate to field conditions will be very helpful in the process of completing construction projects. Research on the construction method for the beam and floor slab construction was carried out at the Majalengka Young Generation Center construction. The casting method for the beams and floor slabs is conventional using ready mix concrete with a slump test value of 12 ± 2. From the results of the research on the construction implementation method for the Majalengka Young Generation Arena, it was found that: beam and floor plate work includes, preparatory work includes measuring work, scaffolding installation and formwork installation, then there is reinforcement/reinforcing work, namely steel fabrication in the project area, then there is work Casting the beams and floor plates using a tower crane. Next, maintenance work is carried out on the concrete mixture during the drying process, namely by watering according to regulations.

ANALYSIS OF LABOR PRODUCTIVITY OF BEAM AND FLOOR PLATE WORK WITH WORK SAMPLING METHOD

Productivity is a crucial parameter in achieving project objectives. This research aims to obtain the value of labor productivity on the beam structure and floor plate in home construction using the work sampling method and analyze the comparison of the labor coefficient to the Analysis of Unit Price of Work (AHSP) in 2022. Experimental quantitative research describes this approach, utilizing information gathered through documentation, questionnaires, and participant observation. Data analysis in this research includes Data Uniformity Analysis, Data Sufficiency Analysis, Labor Utilization Rate (LUR), and Spearman Correlation. The findings revealed that the beam concreting job in home construction achieved a labor productivity rate (LUR) of 61.61% and 84.417 kg/person. In the floor plate concreting work, labor productivity reached 98.787 kg/person with a LUR value of 54.10%. Meanwhile, in formwork work, labor productivity reached 3.577 m/person with a LUR value of 53.37%, and in casting work, labor productivity reached 1.287 m/hour with a LUR value of 53.37%. Correlation analysis shows a positive relationship between Labor Utilization Rate and labor coefficient in concreting and formwork works. This indicates that the more efficient the labor, the higher the productivity in carrying out these activities, and the smaller the coefficient value obtained

Perencanaan Ulang Bangunan Gedung Dormitory Kawasan Sains dan Teknologi (KST) Nuklir Yogyakarta Berbasis Building Information Modelling (BIM) 5D

Building Information Modeling (BIM) is a method of processing data during the life cycle of a construction project that uses three-dimensional, real-time, and dynamic building modeling software, thereby creating a complete model related to project data from the planning to the construction phase of the project. In the re-planning of the Yogyakarta Nuclear Science and Technology (KST) Dormitory Building based on Building Information Modeling (BIM) 5D, a special moment resisting frame system (SRPMK) structure was used. The planned building consists of 7 floors, including the lower structure consisting of a 50x50 square pile foundation and five types of pile cap, the upper structure consisting of columns, beams, and floor plates. The quality of concrete in the structure used is fc' 40 Mpa and 35 Mpa with reinforcing steel quality fy 420B. In this planning, 2D and 3D modeling was carried out. Structural analysis was carried out using the SAP2000 application, which was then continued with 2D 3D modeling and Quantity take-off results using Revit and Scheduling using Ms. Projects. The Draft Budget (RAB) results for this planning are Rp. 37,691,368,000.00. The planned duration of work is 26 weeks. The results of the scheduling and RAB are then integrated using the Naviswork software so that the simulation results of the work to be planned can be seen.

Comparative Analysis of The Implementation Of Conventional And Modular Methods On Walls And Floor Plates In Terms Of Cost And Time (Case Study: Construction Of Satpol PP Building)

Building construction requires selecting efficient construction methods to achieve the desired project objectives. This research aims to compare conventional and modular construction methods in the implementation of walls and floor plates, with a focus on cost and time analysis. The case study taken is the construction of the Satpol PP Building. This research method uses a quantitative approach by collecting primary and secondary data. Primary data was obtained through field observations and interviews with parties involved in the project, while secondary data was obtained from project documents, literature and other related references. Data analysis was carried out with comparing the total costs and time required for each construction method.

Analysis of Floor Work Design in Ceramic Factory Building Expansion Project PT, Surya Multi Cemerlang

The development of science and technology must be followed by an increase in the quality of quality Human Resources (HR), who think and act practically and efficiently. It is expected that quality Human Resources (HR) can be born from Universities in Indonesia. Surabaya State University is one of the institutions for the study and development of science, which plays a role in preparing professional workers. Not only equipping students with theoretical knowledge alone, but also equipped with practicums and internship activities as a means of training and skills for various fields according to the majors of each student. Internship Work Practice aims for students to be able to distinguish between the theory obtained in the lecture hall and field work practice, and to be able to think critically, logically, conceptually and applicatively as well as professionally in their fields. In this case, in the Building Engineering Education Study Program, Internship Work Practice is one of the requirements to complete the semester VI courses which are carried out for approximately 4 months in the field, namely the implementation starting from February 14, 2024 to June 14, 2024. To meet these requirements, the author and several fellow students conducted an Internship Work Practice . This Internship Work Practice activity was carried out at the PT Surya Multi Cemerlang ceramic factory, precisely at Jl. Raya Semambung No.296, Semambung-Wonoayu, Sidoarjo with PT Bangun Karya Perkasa Jaya Tbk as the Implementing Contractor. In connection with the many types of work on the Ceramic Factory Building Expansion Project (PT. Surya Multi Cemerlang) in Wonoayu, Sidoarjo, the types of work reviewed are limited to reviewing only the main activities, namely Floor Plate Work. One of the important structural elements in this project is the Floor Plate. As a structural element that functions as a place to support human and object loads, the Floor Plate is very influential in the planning and implementation of building construction. In its planning and implementation, the Floor Plate is designed as well and optimally as possible so as not to cause discomfort or danger. Improper planning and implementation of Floor Plate construction can cause cracks in one piece, which can affect the quality and durability of the floor plate. There are several students who review the implementation of other structural work from several areas being worked on by PT Bangun Karya Perkasa Jaya, such as reviewing the implementation of pile work, reviewing the implementation of Machine Foundation work, and reviewing the implementation of Wall work. Therefore, in the practical work that has been carried out, the author decided to analyze the method of "implementing Floor Plate work in the construction of a ceramic factory" in the field for writing the practical work report.

Model Test and Numerical Simulation of Two Typical Close-Fitting Pile–Wall Integrated Structures in Deep Excavation

Compared to conventional support methods, the close-fitting pile–wall integration technique features a minimized construction spacing between the retaining pile and the basement retaining wall. This approach leverages the pile stiffness to minimize the wall thickness and enhance underground space utilization. However, it currently lacks significant discussions and measured data about the interaction laws between the pile and the wall. The model test and finite element method (FEM) are employed to study the deformation and internal force interaction laws of two typical close-fitting pile–wall integrated structures, and a comparison with conventional design is conducted. Furthermore, this study separately investigates the impact of sensitivity factors, specifically the pile–wall stiffness ratio and floor plate stiffness, on both structures during the basement construction and serviceability stages. The test results can closely match the numerical simulation. The study results reveal that the wall impacts the bending moment of the pile to some extent. The internal force in the wall is significantly influenced by the lateral deformation of the pile and the floor plate. Compared to conventional designs, this structure significantly reduces the bending moment of the wall, particularly in the composite structure. Additionally, the analysis of sensitivity factors reveals their considerable influence on the pile–wall interaction.

Clinical diagnosis and treatment analysis of spontaneous cerebrospinal fluid otorrhoea

Objective:To investigate the clinical features, imaging findings, surgical methods， diagnostic and treatment experience of spontaneous cerebrospinal fluid otorrhoea. Methods:The clinical data of 11 patients with spontaneous cerebrospinal fluid otorrhoea treated surgically at our hospital from May 2018 to May 2023 were retrospectively analyzed. The medical data included medical history, imaging data, leak location, surgical repair method, treatment effect and postoperative follow-up. Results:Among the 11 surgical patients, 4 patients were initially diagnosed with secretory otitis media, 1 was initially diagnosed with purulent otitis media, and 5 patients had a history of meningitis or presented because meningitis as the initial diagnosis. There were 2 cases of cerebrospinal fluid leakage repaired through the ear canal pathway and 9 cases of cerebrospinal fluid leakage repaired through the mastoid pathway. During the operation, leaks were located in the stapes floor plate in 4 cases, sinus meningeal angle in 1 case, posterior cranial fossa combined with middle cranial fossa in 1 case, middle cranial fossa in 4 cases, and labyrinthine segment of the internal auditory canal and facial nerve canal in 1 case. Ten patient was successfully repaired, and another patient developed intracranial hypertension after surgery, with symptoms alleviated by a lateral ventriculoperitoneal shunt. Postoperative follow-up ranged from 6 months to 4 years, and there was no CSF otorrhoea and meningitis recurrence. Conclusion:The incidence of spontaneous cerebrospinal fluid otorrhea is low, the clinical symptoms are atypical, and the rate of delayed diagnosis or missed diagnosis and misdiagnosis is high. Surgery is currently the preferred treatment for spontaneous cerebrospinal fluid otorrhoea, and satisfactory results are usually achieved; During diagnosis and treatment, it is crucial to be vigilant for intracranial hypertension to prevent serious complications and irreversible damage.

Midline Assembloids Reveal Regulators of Human Axon Guidance.

Organizers are specialized cell populations that orchestrate cell patterning and axon guidance in the developing nervous system. Although non-human models have led to fundamental discoveries about the organization of the nervous system midline by the floor plate, an experimental model of human floor plate would enable broader insights into regulation of human neurodevelopment and midline connectivity. Here, we have developed stem cell-derived organoids resembling human floor plate (hFpO) and assembled them with spinal cord organoids (hSpO) to generate midline assembloids (hMA). We demonstrate that hFpO promote Sonic hedgehog-dependent ventral patterning of human spinal progenitors and Netrin-dependent guidance of human commissural axons, paralleling non-human models. To investigate evolutionary-divergent midline regulators, we profiled the hFpO secretome and identified 27 evolutionarily divergent genes between human and mouse. Utilizing the hMA platform, we targeted these candidates in an arrayed CRISPR knockout screen and reveal that GALNT2 , a gene involved in O-linked glycosylation, impairs floor plate-mediated guidance of commissural axons in humans. This novel platform extends prior axon guidance discoveries into human-specific neurobiology with implications for mechanisms of nervous system evolution and neurodevelopmental disorders.

β-arrestins Are Scaffolding Proteins Required for Shh-Mediated Axon Guidance.

During nervous system development, Sonic hedgehog (Shh) guides developing commissural axons toward the floor plate of the spinal cord. To guide axons, Shh binds to its receptor Boc and activates downstream effectors such as Smoothened (Smo) and Src family kinases (SFKs). SFK activation requires Smo activity and is also required for Shh-mediated axon guidance. Here we report that β-arrestin1 and β-arrestin2 (β-arrestins) serve as scaffolding proteins that link Smo and SFKs in Shh-mediated axon guidance. We found that β-arrestins are expressed in rat commissural neurons. We also found that Smo, β-arrestins, and SFKs form a tripartite complex, with the complex formation dependent on β-arrestins. β-arrestin knockdown blocked the Shh-mediated increase in Src phosphorylation, demonstrating that β-arrestins are required to activate Src kinase downstream of Shh. β-arrestin knockdown also led to the loss of Shh-mediated attraction of rat commissural axons in axon turning assays. Expression of two different dominant-negative β-arrestins, β-arrestin1 V53D which blocks the internalization of Smo and β-arrestin1 P91G-P121E which blocks its interaction with SFKs, also led to the loss of Shh-mediated attraction of commissural axons. In vivo, the expression of these dominant-negative β-arrestins caused defects in commissural axon guidance in the spinal cord of chick embryos of mixed sexes. Thus we show that β-arrestins are essential scaffolding proteins that connect Smo to SFKs and are required for Shh-mediated axon guidance.

Developmental Anatomy of Gray Matter of Spinal Cord in Animals with Special Reference to Prenatal Goat: A Review

Spinal cord is the major nerve tract of vertebrates, extending from the brain through the vertebral canal. Primordium, the neural tube was initially with thick lateral walls having three layers: inner germinal/ependymal layer, middle mantle layer forming the gray matter and outer marginal layer forming white matter. It presented a diamond-shaped lumen with a sulcus limitans, alar, basal, roof and floor plates and limiting membranes. Gray matter of the cord occupies the central region of spinal cord and in amniotes has in cross section, the well-known ‘H’ or butterfly-like shape. The white matter forms a thick peripheral zone of the cord and is divided by the gray columns into dorsal, lateral and ventral funiculi. There are three coumns for the gray matter, viz. the dorsal, ventral and lateral horns, with ventral ones being wider at the enlargements of the cord. Lateral horn appears only at thoracic, anterior lumbar and middle sacral levels. The layers of gray matter were laminae I to X. Lamina I or marginal zone capped the dorsal horn. Lamina II represented substantia gelatinosa and covered the dorsal horn, beneath marginal zone. Lamina III was an area with loosely arranged larger cells, entering the substantia gelatinosa. Lamina IV was a poorly defined cell column with nucleus proprius. Lamina V was a broad zone extending across the cervix of the dorsal horn; had spinal reticular nucleus and reticular processes. The region showed small to medium-sized cells which were most numerous in the zone between the ventral part of the dorsal horn and the lateral funiculus of white matter. Lamina VI was broad with unclear boundaries, related medially to central canal. It had compact medial and less compact lateral zones. Lamina VII shows the intermediate gray matter, with intermediolateral and intermediomedial nuclei, cervical nucleus of Stilling and Clark’s column. Lamina VIII had cells of small and medium size and was not sharply distinguished from lamina VII. Lamina IX had alpha and gamma type of neurons, which were larger at enlargements. Ventral horn had lateral and medial nuclear groups of multipolar neurons. Medial nuclear groups were seen in all regions and lateral groups only at enlargements. Medial nuclear group had dorsomedial and ventromedial nuclei; whereas the smaller lateral nuclear group had dorsolateral and ventrolateral nuclei. In addition, the enlargements also had central and retrodorsolateral nuclei. Lamina X was seen around the central canal. Laminae varied in cell size and thickness between regions of the cord. Development of laminae and nuclei of spinal gray in fetuses corresponded to the progressive growth of muscles and skeleton. All the ten laminae were together present in the spinal gray matter by the end of gestation.

Impaired Sonic Hedgehog Responsiveness of Induced Pluripotent Stem Cell-Derived Floor Plate Cells Carrying the LRRK2-I1371V Mutation Contributes to the Ontogenic Origin of Lower Dopaminergic Neuron Yield.

Lower population of dopaminergic (DA) neurons is known to increase susceptibility to Parkinson's disease (PD), and our earlier study showed a lower yield of DA neurons in Leucine-Rich Repeat Kinase Isoleucine 1371 Valine (LRRK2-I1371V) mutation-carrying PD patient-derived induced Pluripotent Stem Cells (iPSCs). Although the role of Sonic Hedgehog (SHH) in DA neurogenesis of floor plate cells (FPCs) is known, the effect of LRRK2 mutations on SHH responsiveness of FPCs impacting DA neuronal yield has not been studied. We investigated SHH responsiveness of FPCs derived from LRRK2-I1371V PD patient iPSCs with regard to the expression of SHH receptors Patched1 (Ptch1) and Smoothened (Smo), in conjunction with nuclear Gli1 (glioma-associated oncogene 1) expression, intracellular Ca2+ rise, and cytosolic cyclic adenosine monophosphate (cAMP) levels upon SHH induction. In addition, we examined the mechanistic link with LRRK2-I1371V gain-of-function by assessing membrane fluidity and Rab8A and Rab10 phosphorylation in SH-SY5Y cells and healthy control (HC) FPCs overexpressing LRRK2-I1371V as well as FPCs. Although total expression of Ptch1 and Smo was comparable, receptor expression on cell surface was significantly lower in LRRK2-I1371V FPCs than in HC FPCs, with distinctly lower nuclear expression of the downstream transcription factor Gli1. HC-FPCs transfected with LRRK2-I1371V exhibited a similarly reduced cell surface expression of Ptch1 and Smo. Intracellular Ca2+ response was significantly lower with corresponding elevated cAMP levels in LRRK2-I1371V FPCs compared with HC FPCs upon SHH stimulation. The LRRK2-I1371V mutant FPCs and LRRK2-I1371V-transfected SH-SY5Y and HC FPCs too exhibited higher autophosphorylation of phospho LRRK2 (pLRRK2) serine1292 and serine935, as well as substrate phosphorylation of Rab8A and Rab10. Concurrent increase in membrane fluidity, accompanied by a decrease in membrane cholesterol, and lower expression of lipid raft marker caveolin 1 were also observed in them. These findings suggest that impaired SHH responsiveness of LRRK2-I1371V PD FPCs indeed leads to lower yield of DA neurons during ontogeny. Reduced cell surface expression of SHH receptors is influenced by alteration in membrane fluidity owing to the increased substrate phosphorylation of Rab8A and reduced membrane protein trafficking due to pRab10, both results of the LRRK2-I1371V mutation.

Inpatient Corridor Visibility and Care Team Communication.

This article aims to investigate the impact of fine-grained differences in unit layout on care staff corridor communication. Corridor design can be undertaken with greater emphasis on space and cost efficiency or with greater emphasis on affording quality knowledge workspace. Building on prior research on care team communication and inpatient unit design, this study aims to gain a better understanding of how corridor layout properties affect care team communication. This study used space syntax analysis to characterize inpatient unit design, specifically floor-plan layout. Two approaches were used to capture care team communication: behavior mapping with recording of care team member locations and whether they were communicating and the HDR CARE Scale, Inpatient Version. The two units were part of a vertical tower expansion project, and, though constructed at separate times, they maintain the same organizational culture, floor plate, and location of key vertical elements. The newer unit was found to be more visually open using three measures from space syntax. More nursing staff verbal communication was observed on this unit. Across both units, nursing staff were more likely to be observed verbally communicating in locations that were more visually connected (i.e., at locations with larger viewsheds). There were no significant differences between nursing staff self-report on work using the HDR CARE Scale, Inpatient Version. We conclude that care team communication may tend to be encouraged by visual connectivity that can be promoted through floor-plan layout.

Forced LMX1A expression induces dorsal neural fates and disrupts patterning of human embryonic stem cells into ventral midbrain dopaminergic neurons

The differentiation of human pluripotent stem cells into ventral mesencephalic dopaminergic (DA) fate is relevant for the treatment of Parkinson's disease. Shortcuts to obtaining DA cells through direct reprogramming often include forced expression of the transcription factor LMX1A. Although reprogramming with LMX1A can generate tyrosine hydroxylase (TH)-positive cells, their regional identity remains elusive. Using an invitro model of early human neural tube patterning, we report that forced LMX1A expression induced a ventral-to-dorsal fate shift along the entire neuroaxis with the emergence of roof plate fates despite the presence of ventralizing molecules. The LMX1A-expressing progenitors gave rise to grafts containing roof plate-derived choroid plexus cysts as well as ectopically induced TH-positive neurons of a forebrain identity. Early activation of LMX1A prior to floor plate specification was necessary for the dorsalizing effect. Our work suggests using caution in employing LMX1A for the induction of DA fate, as this factor may generate roof plate rather than midbrain fates.

Testing of Concrete Structures with Non-Destructive Test Method (NDT) Using Ultrasonic Pulse Velocity (UPV) at the Building on the Ancol Beach

The UPV test procedure with the PUNDIT device is set based on the concept of the wave flow speed passing through a solid object bound to the elastic properties of a tangible medium. When used properly and correctly, this tool will provide a lot of information about the condition of the surface or the inside of the concrete. Classification for pulse velocity results according to the speed criteria of concrete quality presented in BS 1881: Section 203:1986 as follows: 4500 m/s = excellent concrete condition, 3400 – 4500 m/s = good concrete conditions, 3000-3500 m/s = medium concrete situation, < 3000 m/ s = concrete problem condition (doubtful concrete condition). The pulse velocity test results are measured by three methods: direct transmission, semi-direct transmission and indirect or surface transmission. The test results for the structure of the beams, cylinders, tie beams, and floor plates of the office building on the edge of the anchor showed that it is in medium concrete condition. The average value of the estimated quality of concrete results of the test UPV Pundit for Beams was 25,29MPa, Slabs was 25,17MPa, Tie Beam was 25,06MPa, and Pilecap was 25,46MPa. The result has met the minimum requirement for concrete solid pressure of 21MPa for the quality of unique structures concrete according to SNI-2847-2019.

Rigid Frame Structure Analysis of Wyndham Hotel Building Palembang

A building that is sturdy, strong, secure, and effective must be designed according to building criteria from all structrural components, starting from columns, beams, floor plates, stairs, foundation, excavations, and basement structures. To build quality that meets the criteria from all aspects. Especially the building under study is a high level building that uses a rigid frame structure where the structure is a rigid connection that connect horizontal beams as well as vertical columns then connected to a plane. And all of this works together so that it is likely that this building meets the criteria for multi-story buildings and guarantees the strength of the building structure to meet its functional aspects.

ANALISIS MANAJEMEN WAKTU KONSTRUKSI PEMBANGUNAN GEDUNG KLINIK UNIBA DENGAN MENGGUNAKAN METODE CPM DAN APLIKASI QM FOR WINDOWS V5

Factors that influence the delay in the construction of the UNIBA clinic building is a lack of communication between labor and supervisor, delay shiping material, quality of job control, the intensity of rainfall. This research is to determine the analysis of the management planning of the construction. To find out the urgency of the use of barchart in the process of managing the construction of the UNIBA clinic building. Conclution in this problem the author uses the CPM method, barchart. In this CPM analysis there are several jobs that belong to the criticalpath, namely the critical path of the work of the UNIBA clinic building structure there are 10 jobs, namely preparatory work (A-7 days). Bored pilee (B-21 days) work. Pile cap (C-21 days). Floor plate work (H-14 days). Ladderwork (-3 days). Beam wor (N-21 days). Floor plate work (S-0-14 daays). Sanitair work (W-35). Ceiling work (S-42). Paint work (X-42). Conclution of the use of barchart in the process of managing the construction of th UNIBA clinic building timeliness, data objectivity, submitting information on time, to provide standards that can be used to measure or compare progress with status, to identify and isolate the most important information

SELECTION OF METHODS OF CARRYING OUT FLOOR PLATE BEKESTING WORK WITH COST COMPARISON

The process of developing an area can begin with infrastructure development, where one example of such infrastructure is a building that has various functions. The Surabaya City X building construction project is one of the objects that will be studied in this study. This project has an estimated value of Rp183.000.000.000.00. With a project value of that size, this study aims to choose alternative materials for floor slab formwork work. Floor plate formwork work is one type of work that results in the waste of material used for formwork. Formwork planning requires consideration of using a method or methods to make it easier and cheaper, especially for buildings that are the same shape as the previous building and are of the same size. Conventional floor plate formwork materials are wood, multiplex, and nails; alternative choices are conventional formwork, semi-system formwork, full-system formwork, and bondek plate formwork. The results of this study indicate that when initially analyzed using conventional formwork, it costs Rp6.421.406.646; when using alternative semi-system formwork, it costs Rp6.002.969.805; using full system formwork costs Rp5.530.690.993; and if using bondek plate formwork, it costs Rp6.116.119.937.

Perencanaan Gedung Bertingkat 5 Lantai Asrama Putri Pondok Pesantren Hasyim Asy’ari Bangsri Jepara

The development of the world of Islamic education in Indonesia also includes Islamic boarding schools, Hasyim Asy'ari Bangsri Jepara Islamic boarding school is one of the best Islamic boarding schools, to support the smooth teaching and learning, it is necessary to have adequate student facilities and facilities. This research aims to help fulfill the capacity of santri facilities and infrastructure at the Hasyim Asy'ari Bangsri Jepara Islamic boarding school. The building structure calculations were reviewed for dead loads, live loads and earthquake loads using the SAP 2000 program. The structural calculations carried out included roofs, floor plates, beams, columns, stairs, sloofs and foundations. The building planning location is Jl. Jepara-Bangsri No. 9, Krasak Bangsri, District. Bangsri, Kab. Jepara, Central Java 59452. From the structural planning, the roof structure is obtained with 2L steel trusses 50.50.7, floor plate 130 mm ∅ 12-100, beam B1 500x300 mm 10 D19, beam B2 400x250 mm 10 D19, column K1 500x500 mm 18 D19, column K2 400x400 mm 12 D19, ladder plate thickness 150 mm ∅ 12-150, sloof 500x300 mm 9 D19, pile cap foundation 160x160x60 mm, pile D30 cm 6 m long, with concrete quality (f'c) = 30 MPa, reinforcement quality (fy) = 400 MPa and (fy) = 240 MPa. The programs used are Microsoft Office, Microsoft Excel, Autocad, SAP 2000, and SketchUp.