Fisheries Research Institute Research Articles

Effects of salinity on osmotic pressure and nonspecific immune enzymes in juvenile Eniocheir sinensis

PDF HTML阅读 XML下载 导出引用 引用提醒 盐度对中华绒螯蟹仔蟹渗透压和非特异性免疫酶的影响 DOI: 作者: 作者单位: 1. 中国水产科学研究院东海水产研究所, 上海 200090;2. 上海海洋大学水产与生命学院, 上海 201306 作者简介: 徐静静(1992-),女,硕士研究生,从事水生动物生理生态学研究.E-mail:xujingdhs@163.com 通讯作者: 中图分类号: S93 基金项目: 农业农村部财政专项（CJDC-2017-23）；中央级公益性科研院所基本科研业务费项目（2016M05）；上海市中华绒螯蟹产业技术体系项目（沪农科产字2017-4号）. Effects of salinity on osmotic pressure and nonspecific immune enzymes in juvenile Eniocheir sinensis Author: Affiliation: 1. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Shanghai 200090, China;2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:设置盐度梯度0、4、8、12、16、20和24，测定不同盐度下中华绒螯蟹（-ATPase活性、氧合血蓝蛋白含量以及超氧化物歧化酶（SOD）、碱性磷酸酶（AKP）、酚氧化酶（PO）活性，研究盐度对仔蟹成活率、渗透压调节和非特异性免疫酶活性的影响，探讨仔蟹对盐度的适应性。结果表明，盐度8实验组中华绒螯蟹仔蟹成活率和氧合血蓝蛋白含量最高，分别为（90.00±1.00）%和（0.253±0.023） mg/mL，显著高于其他实验组（+浓度，Na+-K+-ATPase活性和非特异性免疫酶活性在盐度0~8时呈下降趋势，而在盐度8~12时逐渐上升，当盐度大于12时趋于稳定；这些指标值在盐度8实验组降到最低，Na+、Cl-和K+浓度分别为（40.897±1.700） mmol/mL、（8.340±2.130） mmol/mL和（1.842±0.158） mmol/mL，Na+-K+-ATPase酶活性为（3.153±0.735） U/mg，非特异性免疫酶SOD、AKP和PO活性分别为（129.026±3.496） U/mg、（1.326±0.173） U/mg和（16.366±0.065） ng/mL。综合各项实验指标表明，不同盐度对中华绒螯蟹仔蟹渗透压和非特异性免疫酶活性具有一定影响，中华绒螯蟹仔蟹在盐度4~8水体中有较好的生理适应性，淡水和高盐度水体会降低其渗透压调节和免疫防御功能，这为长江口仔蟹资源变动评估和繁育场保护提供了参考。 Abstract:Salinity is an important ecological factor in the environment. It not only affects growth but also survival rate, osmotic pressure, and non-specific enzymes in juveniles of E. sinensis in the Yangtze Estuary, the effects of salinity on survival rate, osmotic pressure, and non-specific enzymes of juvenile were studied. Juveniles were added to circular glass fiber cylinders with different salinity levels (0, 4, 8, 12, 16, 20, and 24) and maintained under the following conditions:average water temperature 23-24; pH 7.2℃ -7.8; and average DO>5 mg/L. The survival rate, tissue homogenate supernatant Na+, Cl-, and K+ concentrations, Na+-K+-ATPase activity, oxyhemoglobin content, and the activities of total superoxidase (SOD), alkaline phosphatase (AKP), and phenoloxidase (PO) were analyzed after 3 days of the experiment. The results showed that the salinity 8 experimental group had the highest survival rate and hemocyanin content, which were 89% and (0.253±0.023) mg/mL, respectively, and were significantly higher than those of other experimental groups (<0.05) but were minimized in the freshwater and salinity 24 groups. Na+, Cl-, and K+ concentrations, Na+-K+-ATPase activity, and non-specific immune enzyme activity were divided into three stages. Firstly, these indicators decreased significantly with increasing salinity (0-8), gradually increased with higher salinity (8-12), and finally stabilized at the highest salinity (12-24). It had its lowest index values in the salinity 8 experimental group, the concentrations of Na+, Cl- and K+ were (40.897±1.700) mmol/mL, (8.340±2.130) mmol/mL, and (1.842±0.158) mmol/mL, and the activity of Na+-K+-ATPase was (3.153±0.735) U/mg, and the activities of SOD, AKP, and PO were (129.026±3.496) U/mg, (1.326±0.173) U/mg, and (16.366±0.065) ng/mL, respectively. Together, the results revealed that the juveniles were highly adaptable in low salinity (4-8), whereas fresh water and high salinity water weakened its osmotic regulation and immune defense function. The goals of this study were to reveal the physiological characteristics during feed-bait migration and provide a scientific basis for resource conservation and environmental assessment of juvenile . 参考文献 相似文献 引证文献

Cloning and prokaryotic expression of Cryptocaryon irritans α-tubulin gene and its mRNA expression levels in different life history stages

PDF HTML阅读 XML下载 导出引用 引用提醒 刺激隐核虫α-微管蛋白基因的克隆、原核表达及在生活史不同阶段的mRNA表达 DOI: 作者: 作者单位: 1. 集美大学水产学院, 福建 厦门 361021;2. 福建农林大学动物科学学院, 福建 福州 350002;3. 莆田市水产科学研究所, 福建 莆田 351100;4. 福建省水产研究所, 福建 厦门 361013 作者简介: 孙嘉阳(1992-),女,硕士研究生,从事分子生物学研究.E-mail:344156662@qq.com 通讯作者: 中图分类号: S917 基金项目: 福建省海洋与渔业厅五新项目（201209200023）；福建省科技厅高校产学合作项目（2017N5002）. Cloning and prokaryotic expression of Cryptocaryon irritans α-tubulin gene and its mRNA expression levels in different life history stages Author: Affiliation: 1. Fisheries College, Jimei University, Xiamen 361021, China;2. Animal Science College, Fujian University of Agriculture, Fuzhou 350002, China;3. Putian Institute of Fishery Sciences, Putian, 351100, China;4. Fujian Fisheries Research Institute, Xiamen, 361013, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:从本实验室已有刺激隐核虫（）转录组数据中筛选获得α-微管蛋白基因片段，利用5'RACE和3'RACE技术首次克隆获得其cDNA，全长为1602 bp，包含1356 bp的开放阅读框，编码451个氨基酸，预测蛋白质分子量为49.78 kD。生物信息学分析表明α-微管蛋白为亲水性非跨膜蛋白，氨基酸序列的第142~148位有特异且保守的GTP核苷酸结合位点（GGGTGSG）。对刺激隐核虫α-微管蛋白氨基酸序列进行同源性比对及进化树分析，发现其与间日疟原虫（Euplotesoct ocarinatus）、尾刺耐格里原虫（）等的序列一致性高达94%~95%，且在系统进化树上聚为一支。采用实时荧光定量PCR技术对α-微管蛋白基因在刺激隐核虫3个生活史阶段的表达进行检测，结果显示α-微管蛋白基因的表达量在纤毛虫时期显著高于包囊和滋养体时期（<0.05）。我们进一步构建了α-微管蛋白重组表达载体，并转化至大肠杆菌表达菌株BL21（DE3）和Rosetta （DE3）中进行原核表达，SDS-PAGE分析表明，诱导表达的重组蛋白分子量约为50 kD，与预测的结果一致，即成功诱导表达α-微管蛋白。本实验结果为后续制备α-微管蛋白有效亚单位疫苗防治刺激隐核虫病奠定了基础。 Abstract:is an extremely destructive ciliate parasite of marine fishes, which results in severe economic costs in mariculture. Its life history is divided into three stages:trophont, tomont, and theront. Tubulin is a major component of the cytoskeleton, cilia, and flagella, which plays an important role in maintaining cell morphology, intracellular trafficking, cell division, ciliary, and flagella motility. At the same time, many studies showed that tubulin has immunogenicity and can be used as a target antigen vaccine. In this study, the α-tubulin gene fragment was obtained from the transcriptome data in our laboratory. Full-length cDNA of α-tubulin was cloned for the first time by 5'RACE and 3'RACE. The results showed that full-length cDNA of α-tubulin is 1602 bp containing a 1356 bp open reading frame, which encodes proteins with 451 amino acids. The predicted molecular mass of α-tubulin is 49.78 kD. Bioinformatics analysis showed that it is a hydrophilic non-transmembrane protein. The amino acid sequence at positions 142-148 has a unique conserved GTP nucleotide binding site (GGGTGSG). Multiple sequence alignment analysis and phylogenetic analysis revealed that the α-tubulin in was integrated with other protozoa in the phylogenetic tree and had 94%-95% sequence identity with Naegleria gruberi, and . The real-time quantitative PCR technique was used to detect the expression of α-tubulin gene in the life history of . The results showed that α-tubulin gene expression was significantly higher in the theront stage than in the tomont and trophont stages ( < 0.05). Furthermore, the α-tubulin recombinant expression vector was constructed and transformed into the BL21 (DE3) and Rosetta (DE3) of the expression strain for prokaryotic expression. SDS-PAGE analysis showed that the molecular weight of the recombinant protein was approximately 50 kD, which was consistent with the predicted result, indicating that α-tubulin protein induced expression successfully. The results of this study laid the foundation for the subsequent preparation of an effective subunit vaccine. 参考文献 相似文献 引证文献

Technologies for large-scale cultivation of giant salamander cells and iridovirus by the Cytodex 3 microcarrier

PDF HTML阅读 XML下载 导出引用 引用提醒 大鲵细胞与大鲵虹彩病毒的微载体规模化培养工艺及优化 DOI: 作者: 作者单位: 1. 华中农业大学水产学院 水生动物医学系, 湖北 武汉 430070;2. 中国水产科学研究院 长江水产研究所, 湖北 武汉 430223 作者简介: 贾路路(1991-),女,硕士,从事水产动物医学研究.E-mail:1525825924@qq.com 通讯作者: 中图分类号: S941 基金项目: 公益性行业（农业）科研专项（201203086-05）. Technologies for large-scale cultivation of giant salamander cells and iridovirus by the Cytodex 3 microcarrier Author: Affiliation: 1. Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;2. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:利用Cytodex 3微载体悬浮培养系统规模化培养大鲵肌肉细胞（GSM）和大鲵虹彩病毒（GSIV），研究了微载体培养GSM细胞的形态和增殖特性，同时测定了病毒在培养系统中的增殖动态相关指标。结果显示，在Cytodex 3微载体培养系统中，将GSM细胞在贴壁期以转速30 r/min，每静置40 min搅拌2 min的方式间歇搅拌，10 h后贴壁率可达95%，培养基中最适血清浓度为10%，最适微载体浓度为2 g/L，最适细胞初始接种密度为1.2×105 cells/mL；增殖期以25 r/min的连续搅拌方式可以达到最佳的细胞生长效能。倒置显微镜与扫描电镜观察结果显示，GSM细胞呈长梭形，紧密贴附在Cytodex 3微载体上，生长良好。采用优化的工艺条件培养GSM细胞，以感染复数（MOI）为0.5的剂量接种GSIV至规模化培养的GSM细胞，48 h后GSM细胞出现典型的细胞病变效应，72 h病毒滴度达到最高TCID50=10-8.50±0.20/mL。本研究为大鲵虹彩病毒病疫苗的规模化生产工艺研究奠定了前期基础。 Abstract:The Chinese giant salamander , a member of the family Cryptobranchidae, is the largest extant amphibian species in the world, which is valued for food, medicine, and research on animal evolution and biodiversity because of its unique phylogenetic position and physiological features. Within the last decade, Chinese giant salamander farming has increased rapidly in China. However, with the rapid development of Chinese giant salamander aquaculture, a severe epizootic recently occurred in cultured Chinese giant salamanders in Hubei, Hunan, Sichuan, Shannxi, and Zhejiang Provinces of China, causing tremendous economic losses. The causative pathogen has been identified as the giant salamander iridovirus (GSIV). At present, no effective methods are available for the control of the disease. Immunoprophylaxis is considered the best measure in controlling viral diseases in aquatic animals, and the large-scale cultivation technology of giant salamander cells and GSIV are of significance for the immunoprophylaxis of the disease. In this study, by using Cytodex 3 microcarriers to culture giant salamander muscle cells (GSM) and GSIV at a large scale, the GSM cell morphology, proliferative characterization, and GSIV growth dynamics were investigated. The results showed that the attachment efficacy reached 95% after 10 h of cultivation with intermittent agitation of 30 r/min for 2 min followed by 40 min still culture during the cell attachment period in the Cytodex 3 microcarrier culture system. The optimal culture conditions are as follows:serum concentration 5%, microcarrier concentration 2 g/L, and initial inoculation cell density of 1.2×105 cells/mL. During the cell growth period, the continuous stirring speed was 25 r/min. Under inverted microscopy and electron microscopy, the GSM cells on the Cytodex 3 microcarriers were long, spindle-shaped, and well adhered. After infection with GSIV at a multiplicity of infection of 0.5, the GSM cells on Cytodex 3 microcarriers showed the typical cytopathic effect at 48 h post infection, and the highest virus titer (TCID50/mL) of 10-8.50±0.20/mL was reached at 72 h post infection. This study established a solid foundation for further investigation on the large-scale technologies of GSIV vaccine production in the future. 参考文献 相似文献 引证文献

Molecular cloning analysis of the 5' regulatory region of Ikaros gene from Oreochromis niloticus and screening of its SNP markers for Streptococcus agalactiae resistance

PDF HTML阅读 XML下载 导出引用 引用提醒 吉富尼罗罗非鱼Ikaros基因5'调控区的克隆、序列分析及抗无乳链球菌相关SNP位点筛选 DOI: 作者: 作者单位: 1. 中国水产科学研究院 珠江水产研究所, 农业部热带亚热带水产资源利用与养殖重点实验室, 广东 广州 510380;2. 上海海洋大学 水产与生命学院, 上海 201306;3. 惠州市渔业研究推广中心, 广东 惠州 516002 作者简介: 陈昆平(1991-),男,硕士研究生,研究方向为水产健康养殖及遗传育种.E-mail:colinchenkunping@163.com 通讯作者: 中图分类号: S917 基金项目: 现代农业产业技术体系专项资金项目（CARS-46）；广东省自然科学基金项目（2016A030313146）；国家自然科学基金项目（31502205）. Molecular cloning analysis of the 5' regulatory region of Ikaros gene from Oreochromis niloticus and screening of its SNP markers for Streptococcus agalactiae resistance Author: Affiliation: 1. Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China;2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;3. Fisheries Research and Extension Center of Huizhou, Huizhou 516002, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:为获得尼罗罗非鱼（）抗链球菌病相关的单核苷酸多态性（single nucleotide polymorphism，SNP）标记，本研究通过染色体步移法克隆了尼罗罗非鱼基因的启动子和转录调控元件进行预测和分析。利用PCR产物直接测序法从亲本（P0）尼罗罗非鱼基因5'调控区中筛查到5个SNPs，分别为SNP1（g.562，G>A）、SNP2（g.217，G>T）、SNP3（g.-53，C>T）、SNP4（g.-220，T>C）和SNP5（g.-579，T>C）。利用Snapshot技术对子一代（F1）尼罗罗非鱼易感群体和抗病群体进行相应SNPs的基因分型，分析两个群体遗传多样性参数，结果显示多态信息含量（polymorphism information content，PIC）值在0.0872~0.3747之间，表明基因5'调控区SNPs与抗链球菌病性状关联分析结果表明，SNP2、SNP3、SNP4和SNP5的基因型频率和等位基因频率在易感群体和抗病群体中存在显著差异（基因5'调控区SNPs可形成1个单倍块和5种单倍型，其中GGCTT单倍型与抗病性显著相关（<0.05）。此外，还发现SNP2和SNP5处于完全连锁状态（Ikaros基因5'调控区筛选到4个抗链球菌病相关SNPs和1个单倍型（GGCTT），均可作为尼罗罗非鱼分子育种的候选分子标记。 Abstract:Tilapia () is one of the most important fishery species in the world and has been introduced and cultured widespreadly because of its fast growth rate, strong reproductive capacity, good adaptability and omnivorous feeding habit. However, disease has become the biggest threat for tilapia breeding. Recently, the epidemic and outbreak of tilapia disease caused substantial economic losses to aquaculture industry. Breeding resistant variety in tilapia was unimportant way to solve the problem of disease. The molecular marker assisted selection (MAS) has become an efficient breeding method for selecting and breeding tilapia, and will accelerate genetic improvement and increase selection intensity for disease resistance. The single nucleotide polymorphism (SNP) markers are used in many genetic and breeding studies because they are abundant in genomes, and can be genotyped easily. Ikaros is a kind of transcription factor with zinc finger structure that is essential to the development of lymphocyte and to the maintenance of normal immune function. Therefore, to obtain large amount of effective SNP molecular genetic markers and to perform MAS for disease resistance in tilapia, it is essential to study immune related candidate and to examine whether the SNPs in the gene are associated with disease resistance. In this study, the 5' regulatory region sequence of , length of 4178 bp, were obtained through Genome Walking method from . Bioinformatics software was used to analyze the 5' regulatory region sequence of gene. The predicted transcriptional start site (TSS) was in the initiation codon (ATG) upstream of 931 bp, and the core promoter regions was located at -57 bp to 48 bp when the TSS was specified as 1. The predicted promoter regions of gene included basic start of substructure components:TATA box, CCAAT box and octamer. The analysis of transcription factor binding sites (TFBS) showed that abundant of TFBS were located at -2200 bp to 1200 bp in 5' regulatory region of gene, such as GATA-1, Homeobox, CDP CR3+HD and AP-1. The analysis of CpG islands showed that two CpG islands were in 5' regulatory region sequence of gene, one of which was located in promoter regions and the other of which was located in the first exon. Five SNPs in the 5' regulatory region of gene were detected by direct sequencing method from the parents (P0), which are named SNP1 (g.562, G>A), SNP2(g.217, G>T), SNP3(g.-53, C>T), SNP4(g.-220, T>C) and SNP5(g.-579, T>C). The five SNPs were sited in various regulatory elements in promoter regions which could have major implications for exact expression of gene. Based on Snapshot method analysis, the frequencies of alleles and genotypes were calculated in susceptible groups and resistant groups of ). The polymorphisms and genetic parameter of the SNPs in resistant groups and susceptible groups were calculated by software Popgen 32 and PIC. The result showed that the polymorphism information content (PIC) of the 5' regulatory region of SNPs was 0.0872~0.3747, suggesting that all the SNPs had moderate of intermediate polymorphism. The correlation between SNPs and resistance to was analyzed by software SPSS 17.0, the results showed that four of them (SNP2, SNP3, SNP4and SNP5) were significantly associated with the resistance to <0.05). Moreover, All of the SNPs in the 5' regulatory region of could formed one haplotype block and five haplotypes from the prediction of linkage disequilibrium analysis used software Haploview 4.2. The haplotypes (GGCTT) were significantly associated with the resistance to <0.05), and two of the haplotypes (GGTCT and GTCCC) were significantly associated with <0.05). Furthermore, the SNP2 and SNP5 were completely linked with each other ('=1), which could be selected as tag SNP for research of genetic breeding in . The results suggest that the four SNPs (SNP2, SNP3, SNP4 and SNP5) and the haplotypes (GGCTT) in the 5' regulatory region of could be potential genetic markers for future molecular selection of . 参考文献 相似文献 引证文献

Analysis of the bacterial community composition of the epiphytes on diseased Gracilaria lemaneiformis using PCR-DGGE fingerprinting technology

PDF HTML阅读 XML下载 导出引用 引用提醒 PCR-DGGE指纹图谱技术分析发病龙须菜附生菌菌群组成 DOI: 作者: 作者单位: 1. 汕头大学 理学院 生物系, 广东省海洋生物技术重点实验室, 广东 汕头 515063;2. 汕头大学 理学院, 汕头大学-马尔凯理工大学藻类联合研究中心, 广东 汕头 515063;3. 福建省水产研究所, 福建省海洋生物增养殖与高值化利用重点实验室, 福建 厦门 361013 作者简介: 裴鹏兵(1990-),男,硕士研究生,研究方向为水生环境保护与修复.E-mail:peipengbing1990@126.com 通讯作者: 中图分类号: S917;Q935 基金项目: 广东省海洋渔业科技与产业发展专项项目（A201405B06）；福建省海洋生物增养殖与高值化利用重点实验室开放课题基金项目（2016fjscq02）. Analysis of the bacterial community composition of the epiphytes on diseased Gracilaria lemaneiformis using PCR-DGGE fingerprinting technology Author: Affiliation: 1. Guangdong Provincial Key Laboratory of Marine Biotechnology, Department of Biology, College of Science, Shantou University, Shantou 515063, China;2. Inauguration of STU-UNIVPM Joint Algal Research Center, College of Science, Shantou University, Shantou 515063, China;3. Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:本研究以07-2品系龙须菜（）为实验材料，提取自然条件下代表不同病变程度的健康、病间、白化藻体的附生菌DNA，经PCR扩增16S rDNA基因的V3可变区并进行变性梯度凝胶电泳（DGGE），分离V3片段，再对目的条带进行克隆鉴定。实验结果经数据化后计算龙须菜附生菌数目并比较附生菌菌群多样性，通过非度量多维尺度分析（nMDS）及多元梯度分析（RDA）来比较附生菌菌群组成相似性及优势菌群。实验结果表明，随着龙须菜病变程度的加深，附生菌菌落数及菌群多样性逐渐增加（<0.05）。nMDS分析结果表明，健康藻体与白化藻体在附生菌菌群组成上存在差异，病间藻体在附生菌菌群组成上与健康藻体更为接近。RDA分析结果表明，龙须菜健康藻体附生菌可能的优势菌群是海洋单胞菌属（）、红杆菌科（Rhodobacteraceae）、假交替单胞菌属（、红杆菌科、玫瑰变色菌属（）。从上述结果可以看出，龙须菜健康藻体与发病藻体在附生菌菌群组成及优势菌上存在较大差异，而介于两者之间的藻体在菌群组成上更偏向于健康藻体。 Abstract:, one of the important sources of agar, is the third most widely cultivated seaweed in China, after . In recent years, there has been a large-scale decrease in the cultivation of , resulting in huge economic losses for the seaweed cultivation industry. Epiphytes grow as an integral part on the surfaces of large algae, and the variations in the seaweed-associated microbial community compositions are typically associated with disease occurrence. In this study, the 07-2 strain was used as experimental material. DNA of epiphytes from the healthy, adjacent, and bleached fronds was extracted, and the V3 variables amplified from 16S rDNA genes were carried out by denaturing gradient gel electrophoresis (DGGE). Finally, the 23 objective bands were cloned and identified. The number and diversity index of the bacterial community of the epiphytes from the three fronds were calculated after digitization of the experimental results. The similarity among the dominant species in the bacterial communities were compared by non-metric multidimensional scaling (nMDS) and redundancy analysis (RDA). The results show that the colony forming units and bacterial community diversity increased gradually with the aggravation in the degree of lesion in <0.05). The nMDS results indicate that the bacterial community composition of the epiphytes varies between the healthy and bleached fronds, while the composition of adjacent fronds is closer compared with that between healthy fronds. The RDA results reveal that the potential dominant bacteria of the epiphytes of the healthy and adjacent fronds are Pseudoalteromonas, respectively. However, the potential dominant bacteria of the epiphytes of the bleached fronds were not identified. The dominant bacteria of the epiphytes of the bleached attached fronds were . It can be seen from the above results that there is a significant difference between the healthy fronds and the diseased fronds of in terms of the bacterial community composition and dominant bacteria of the epiphytes, while the epiphytic bacterial community composition of the adjacent fronds was more similar than that of the healthy fronds. 参考文献 相似文献 引证文献

DNA barcoding: A potential tool for fishery biodiversity conservation

PDF HTML阅读 XML下载 导出引用 引用提醒 DNA条形码在渔业生物多样性保护中的应用 DOI: 作者: 作者单位: 1. 中国水产科学研究院黄海水产研究所, 山东 青岛 266071;2. 青岛海洋科学与技术国家实验室, 海洋渔业科学与食物产出过程功能实验室, 山东 青岛 266200;3. 中国水产科学研究院, 北京 100141 作者简介: 柳淑芳,研究员,从事渔业生物学研究.E-mail:liusf@ysfri.ac.cn 通讯作者: 中图分类号: S917 基金项目: 科技部科技基础性工作专项（2013FY110700）；中央级公益性科研院所基本科研业务费专项（2014A11）；山东省泰山学者建设工程专项. DNA barcoding: A potential tool for fishery biodiversity conservation Author: Affiliation: 1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;2. Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266200, China;3. Chinese Academy of Fishery Sciences, Qingdao 100141, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:生物多样性与人类存亡休戚相关，保护生物多样性是实现人类社会可持续发展的基础。本文立足我国渔业生物多样性现状，针对我国渔业生物过度开发和保护不足等难题，提出了通过开展渔业生物DNA条形码研究来推动我国渔业生物多样性学科发展的思路，详细阐述了DNA条形码在渔业生物资源可持续开发、物种分类鉴定、生物多样性监测、外来物种评价、水产品市场监管、渔业信息化等多个领域的应用现状，进一步展望了DNA条形码可以成为渔业生物区系研究、水域生态研究、电子分类技术以及宏DNA条形码新技术等多个方面的研究热点，呼吁扎实推进我国渔业生物DNA条形码数据库和信息化平台的建设和共享。 Abstract:The Earth's biodiversity are closely altered by a variety of human activities at all scale from local to global. These changes have a strong potential effect to biological communities, ecosystem properties, social affairs and economic development they provide. China fishery is characterized by high biodiversity which cover 10 percent of the total earth species because its role geographical position that occupies the eastern part of the Eurasian continent adjacent to the western Pacific Ocean. In recent years, it is a very realistic and important scientific problem to protect and rationally utilize the biodiversity of fisheries globally. However, insufficient protection and hard utilization of fishery isn't a single case in China. To promote the development of fishery biological diversity in China, this paper puts forward the idea of DNA bar code methods for performing in fishery biology activities. It reviewed how DNA barcode expounds the sustainable development in the fishery biological resources, species identification and classification, fishery biodiversity monitoring, foreign species evaluation, market and supervision, fishery information and so on. Besides that, it further discussed the recent prospects for the DNA barcode in the biologic study of fishery organisms, water ecological research, electronic classification technology and the new technology of meta DNA barcoding. This research had appealed the continuous researches on construction of a fishery DNA barcode database and information platform of China which need to be constructed and shared nationally and globally. 参考文献 相似文献 引证文献

Temporal variation in the composition and abundance of fish larvae and juveniles off the Yangtze River Estuary in spring and summer

PDF HTML阅读 XML下载 导出引用 引用提醒 春夏季长江口邻近水域仔稚鱼种类组成和丰度的月变化 DOI: 作者: 作者单位: 中国水产科学研究院东海水产研究所, 农业部东海渔业资源开发与利用重点实验室, 上海 200090 作者简介: 李建生(1976-),副研究员,从事渔业资源与生态学研究.E-mail:jianshengli@sina.com 通讯作者: 中图分类号: S931 基金项目: 国家公益性行业（农业）科研专项经费项目（201303047）. Temporal variation in the composition and abundance of fish larvae and juveniles off the Yangtze River Estuary in spring and summer Author: Affiliation: East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, Shanghai 200090, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:利用2015年4—7月在长江口邻近水域的调查数据，对春夏季该水域仔稚鱼种类组成和丰度的月变化特征进行了观测。结果表明，共采获62种、35839尾仔稚鱼，分别属于14目、30科、45属。各月种类数范围为22~36种，从4月到6月，先是缓慢增加然后快速增加，至6月达到高峰期，7月则大幅降低。各月的丰度范围为11.45~114.31 ind/（100 m3）；丰度随时间的推移而持续增加。各月主要种类数范围为5~13种，具有较大的波动性；优势种主要为小黄鱼（Chaeturichthys hexanema）、鳀（Acanthogobius ommaturus）、（鱼衔）属（ sp.）等。优势种随时间推移表现出较大的更替性。种类数以20 m以浅水域最多，不同水深范围的月变化有一定的差异性。20 m以浅海域的种类数与表层水温的关系在4—6月为显著正相关（=-0.91，<0.05）。从春季到夏季，仔稚鱼高丰度区从浅水区向深水区转移。 Abstract:The present study investigated the monthly variation in species composition and abundance of fish larvae and juveniles from April to July 2015 off the Yangtze River Estuary. A total of 35839 larvae and juveniles were collected, and these included 62 species from 45 genera, 30 families, and 14 orders. Species richness ranged from 22 to 36, depending on month, increased from April to June, and then declined sharply in July. Meanwhile, the total abundance of larvae and juveniles ranged from 11.45 to 114.31 individuals per 100 m3 and increased throughout the study period, with small increases during the first three months and a greater increase in July. The number of dominant species varied by month, ranging from 5 to 13, and the dominant species mainly included Chaeturichthys hexanema, Engraulis japonicus, Odontamblyopus rubicundus, Acanthogobius ommaturus, and sp.. The dominant species showed greater replacement over time. The greatest species richness was observed in the nearshore water (<20 m depth), and the spatial pattern of species richness along the depth gradient varied among months. Surface temperature and species richness were positively correlated (<0.05) from April to June but negatively correlated from June to July, and in every month, abundance and surface salinity were negatively correlated (<0.05) in the nearshore water (<20 m depth). The high-abundance region shifted away from the shore over time. 参考文献 相似文献 引证文献

PAE coding system-based ethogram of Schizothorax wangchiachii

PDF HTML阅读 XML下载 导出引用 引用提醒 基于PAE编码系统的短须裂腹鱼行为谱 DOI: 作者: 作者单位: 中国水产科学研究院 长江水产研究所, 农业部淡水生物多样性保护重点实验室, 湖北 武汉 430223 作者简介: 朱挺兵(1987-),男,博士,助理研究员,主要从事鱼类生态学研究.E-mail:zhutb2008@163.com 通讯作者: 中图分类号: Q958 基金项目: 国家自然科学基金项目（31402303）. PAE coding system-based ethogram of Schizothorax wangchiachii Author: Affiliation: Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture;Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:短须裂腹鱼（）是长江上游特有鱼种。近年来，由于受过度捕捞、水电开发等人类活动影响，短须裂腹鱼自然资源量不断下降。2015年11月-2016年3月，采用焦点动物取样法和随机取样法对短须裂腹鱼的行为谱进行了研究。以"姿势、动作和环境"为轴心，以生态功能为分类依据，构建短须裂腹鱼PAE行为谱。共辨识和定义了自然条件下短须裂腹鱼的10种姿势、22种动作和43种行为。根据行为的生物学功能，将上述行为划分为11大类，即摄食与排遗、探索、领域、发情、交配、护幼、冲突、运动、聚群、休息和杂类行为；区别了各种行为在雄性、雌性和幼体之间的相对发生频次以及发生季节。本研究有助于短须裂腹鱼的行为与保护研究，亦可为其他鱼类的行为谱研究提供参考。 Abstract:is an endemic fish in the upper Yangtze River. Due to overfishing, hydropower development and other human activities, the natural resources of have decreased during recent decades. The significance of behavioral studies for animal protection is widely accepted. The compilation and analysis of ethograms is the basis of further research on animal behavior. Due to variations in living environments, accurate ethograms of fish should be based on behaviors obtained from natural or semi-natural conditions. The present study aims to establish the behavioral ethogram of in nature. From November 2015 to March 2016, field behavioral observations of were conducted in the Guosheng River, which is located in Sichuan Province, China. A short reach of the Guosheng River, with a length of about 100 m and width of 10 to 13 m, was enclosed as the observation site. A total of 56 pairs of mature parents were released to the enclosure. The river could pass through the enclosure, but experimental fish could not escape. The behaviors of were recorded using underwater cameras and sampled by focal animal and was coded under the "posture, act, and environment" (PAE) coding system. A total of 10 postures, 22 acts, and 43 behaviors were recorded. According to biological functions, the recorded behaviors of were divided into 11 types, including ingestion and elimination, exploration, territoriality, estrus, mating, epimeletic, conflict, locomotion, aggregation, rest and sundry. The relative frequency and occurrence season of each behavior in relation to gender and juvenile were also given out. The behaviors of the juveniles were relatively simple and mainly related to ingestion, elimination, aggregation, and resting. The behaviors of matured were diversified with obvious gender differences, especially in the reproductive behaviors. During the reproductive season, female spent most of the time resting and showed few behaviors such as swimming or mating. While male showed various behaviors, especially endemic behaviors like territorial behavior, sneak mating, and epimeletic behavior. Gender differences in behaviors may reflect the reproductive strategy of , in which more energy is allocated for oocyte development in females and for behavior in males. The behavioral types of aquatic animals are significantly fewer than that of terrestrial higher animals, which may relate to the lower evolutionary level and simpler living environment of aquatic animals. The present study may enable further behavioral and protection research in , and give a reference to behavioral ethogram studies in other fish species. 参考文献 相似文献 引证文献

Microsatellite genetic analysis of gynogenetic grass carp group and two common grass carp groups

PDF HTML阅读 XML下载 导出引用 引用提醒 雌核发育草鱼群体及两个普通草鱼群体的微卫星遗传分析 DOI: 作者: 作者单位: 山东省淡水渔业研究院, 山东省淡水水产遗传育种重点实验室, 山东 济南 250017 作者简介: 朱树人(1986-),男,博士,研究方向为水产动物遗传育种.E-mail:zhshr229@163.com 通讯作者: 中图分类号: S917 基金项目: 国家自然科学基金项目（31440090）；国家大宗淡水鱼产业技术体系济南综合试验站项目（CARS-45-43）；山东省农业良种工程项目（2014lz042）；山东省现代农业产业技术体系鱼类创新团队项目（SDAIT-14-011-01）；山东省重点研发计划项目（2018GNC110034）. Microsatellite genetic analysis of gynogenetic grass carp group and two common grass carp groups Author: Affiliation: Shandong Provincial Key Laboratory of Freshwater Genetics and Breeding, Shandong Freshwater Fisheries Research Institute, Jinan 250017, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:以草鱼（）养殖群体（YZ）为母本，以紫外线灭活的鲤鱼精子激活草鱼卵子，冷休克抑制第二极体排出的方法诱导获得异精雌核发育草鱼群体（CH）。利用12对微卫星引物对YZ群体、YS （扬州广陵长江系家鱼原种场引进草鱼群体）群体、CH群体进行PCR扩增并分析，共检测出194个等位基因，其中75.8个有效等位基因。YZ群体、YS群体、CH群体的平均等位基因数依次为13.0、12.6、4.7；平均有效等位基因数依次是7.7、6.6、2.3；平均期望杂合度依次为0.87、0.82、0.56；平均多态信息含量依次为0.84、0.79、0.49。从每个个体在微卫星位点的纯合率看，YZ群体中个体的纯合度在0.00~0.33，YS群体中个体的纯合度在0.00~0.42，CH群体中个体的纯合度在0.42~0.92。这表明与YZ群体和YS群体相比，CH群体的遗传多样性显著下降，并且在每个位点的纯合率CH群体均高于普通草鱼群体，表明人工诱导减数雌核发育可加速草鱼大多数基因位点的纯合，是快速建立高纯品系的有效手段。同时，本研究筛选并利用微卫星位点组合建立了雌核发育草鱼子代不同家系及其母本亲缘关系的简易、高效鉴别技术，旨在为雌核发育草鱼标记辅助育种打下基础。 Abstract:We induced the gynogenetic offspring of the breeding grass carp (YZ) using UV-inactivated grass carp sperm and cold shock method to inhibit extrusion of the second polar body. Twelve pairs of microsatellite primers were used for amplification and genetic analysis of the YZ population and control population (YS). A total of 194 alleles were detected in the YZ and YS populations, of which 75.8 were effective alleles. The average number of alleles in the YZ, YS and CH populations was 13.0, 12.6, and 4.7, respectively; the average number of effective alleles was 7.7, 6.6, and 2.3, respectively; the average expected heterozygosities were 0.87, 0.82, and 0.56, respectively; and the average polymorphism information content was 0.84, 0.79, and 0.49, respectively. Homozygosity was analyzed for the microsatellite loci:the homozygosity of individuals in the YZ population was 0.00-0.33, the homozygosity of individuals in the YS population was 0.00-0.42, and the homozygosity of individuals in the CH population was 0.42-0.92. The results showed that the genetic diversity of the CH population was significantly lower than that of the YZ population and the YS population, and the homozygosity of the CH population at each locus was higher than that of the common grass carp groups. This suggests that artificially induced meiotic gynogenesis can accelerate the homozygosity of most grass carp gene loci and is an effective means of rapidly establishing a high purity strain. At the same time, this study screened and used microsatellite loci combinations to establish a simple and efficient identification technique for the genetic relationship among different families and their female parent, laying a foundation for the marker-assisted breeding of gynogestidium. 参考文献 相似文献 引证文献

Population genetic structure of Thamnaconus septentrionalis in China's coastal waters based on mitochondrial Cyt b sequences

PDF HTML阅读 XML下载 导出引用 引用提醒 基于线粒体Cyt b基因序列的绿鳍马面鲀6个野生群体的遗传结构分析 DOI: 作者: 作者单位: 1. 中国水产科学研究院黄海水产研究所, 山东 青岛 266071;2. 农业农村部外海渔业开发重点实验室, 广东 广州 510300;3. 中国水产科学研究院南海水产研究所, 广东 广州 510300;4. 青岛海洋科学与技术国家实验室, 海洋渔业科学与食物产出过程功能实验室, 山东 青岛 266071;5. 上海海洋大学 水产与生命学院, 上海 201306 作者简介: 边力(1988-),助理研究员,博士,研究方向为鱼类遗传育种.E-mail:bianli@ysfri.ac.cn 通讯作者: 中图分类号: Q347;S931.5 基金项目: 农业部外海渔业开发重点实验室开放课题（LOF 2017-05）；中国水产科学研究院黄海水产研究所级基本科研业务费项目（20603022017014）. Population genetic structure of Thamnaconus septentrionalis in China's coastal waters based on mitochondrial Cyt b sequences Author: Affiliation: 1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;2. Key Laboratory of Open-Sea Fishery Development, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China;3. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;4. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;5. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:利用线粒体细胞色素b（cytochrome b，Cyt ）6个野生群体（大连、秦皇岛、蓬莱、日照、舟山和汕头）的遗传多样性和群体遗传结构。结果显示，在176个个体915 bp的Cyt 部分序列中共检测到32个变异位点和30个单倍型；6个群体的单倍型多样性为0.883~0.953，核苷酸多样性0.0032~0.0039；群体间固定指数较小，呈中低度分化；AMOVA分析表明，遗传变异主要来源于群体内，群体间遗传分化未达到显著水平。群体历史动态分析表明，绿鳍马面鲀在16.9万~42.2万年前经历种群扩张。总体上，各群体间的遗传分化较小，并未形成相应的地理支系，推测黑潮的输送作用以及绿鳍马面鲀的洄游习性维持了各群体间高强度的基因交流；中更新世中晚期的气候波动对绿鳍马面鲀的种群扩张以及地理分布格局可能具有重要影响。本研究结果加深了人们对于绿鳍马面鲀资源情况的认识，为绿鳍马面鲀资源的保护和合理利用提供了理论依据。 Abstract:Genetic data have aided fishery conservation research and management by facilitating the detection of genetically distinct populations, the measurement of genetic connectivity, and the identification of the risks associated with demographic change and inbreeding. is an important economic marine fish species in China and inhabits in the Bohai Sea, Yellow Sea, East China Sea and South China Sea. has become an important fishing target in the East China Sea since 1974. Its annual catches were once second only to has declined sharply since 1990 due to overfishing and ocean environmental change. To provide better protection and effective recovery for this species, it is urgent to evaluate its population structure and genetic diversity. However, population surveys of in China's coastal waters were rare. No population structure research based on molecular makers was available. In this study, we amplified the partial mitochondrial Cyt gene sequences of 176 individuals from six populations (Dalian, Qinhuangdao, Penglai, Rizhao, Zhoushan, and Shantou). The results showed that 32 polymorphic nucleotide sites and 30 haplotypes were detected among all the Cyt sequences. The haplotype diversity ranged from 0.883 to 0.953, and the nucleotide diversity was 0.0032-0.0039. This genetic diversity pattern was probably caused by a recent demographic expansion in the Pleistocene, a rapid population growth after 1970, and the adaptable biological characteristics of . Although the haplotype diversity was high, the genetic diversity is still vulnerable to overfishing. AMOVA analysis showed that the genetic differentiation of mainly occurred within the population variation. The genetic differentiations among populations were weak and no groups were identified. The high gene exchange among populations is probably caused by the seasonal migration behaviors of the adults and the long-distance transportation of juveniles by the Kuroshio (Black) Current. Demographic history analysis showed that the six populations experienced rapid population growth 0.17-0.42 Mya. This time point was within the middle Pleistocene. During this period, sea levels changed dramatically due to unstable temperatures. We speculate that the sea level changes during the middle Pleistocene might have had significant influence on the population expansion of . To our knowledge, this is the first population genetics research on in China's coastal waters based on molecular markers. This study could deepen our understanding of how to optimize the utilization of the natural resources of . 参考文献 相似文献 引证文献

Genome-wide genetic analysis of cultured rainbow trout ( Oncorhynchus mykiss ) populations in China

PDF HTML阅读 XML下载 导出引用 引用提醒 国内虹鳟代表性养殖群体的高通量SNP芯片检测及遗传分析 DOI: 作者: 作者单位: 1. 中国水产科学研究院, 农业部水生动物基因组学重点实验室, 渔业生物技术北京市重点实验室, 北京 100141;2. 中国水产科学研究院黑龙江水产研究所, 黑龙江 哈尔滨 150070;3. 四川农业大学 动物科技学院, 四川 雅安 625014;4. National Center for Cool and Cold Water Aquaculture, ARS-USDA, Kearneysville, WV 25430, USA;5. 厦门大学 海洋与地球学院, 福建省海洋生物资源开发利用协同创新中心, 福建 厦门 361102 作者简介: 赵紫霞(1983-),女,博士,副研究员,从事水产生物技术研究. 通讯作者: 中图分类号: S917 基金项目: 国家科技支撑计划项目（2015BAD25B01）；中国水产科学研究院基本科研业务费专项（2015C007）. Genome-wide genetic analysis of cultured rainbow trout (Oncorhynchus mykiss) populations in China Author: Affiliation: 1. Key Laboratory of Aquatic Genomics, Ministry of Agriculture;Beijing Key Laboratory of Fishery Biotechnology, Chinese Academy of Fishery Sciences, Beijing 100141, China;2. Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;3. College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China;4. National Center for Cool and Cold Water Aquaculture, ARS-USDA, Kearneysville, WV 25430, USA;5. Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources;College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:本研究旨在对国内虹鳟（）代表性养殖群体开展全基因组水平的遗传评估。利用57K单核苷酸多态性（single nucleotide polymorphism，SNP）芯片，检测了来自不同地域的6个虹鳟养殖群体样本共计48尾，包括黑龙江虹鳟、黑龙江金鳟、四川虹鳟、四川金鳟、北京虹鳟和北京金鳟，共获得有效SNP位点50201个，在中国虹鳟中的多态比例达到97.7%，表明该芯片虽然基于美国和挪威虹鳟群体设计，但对中国群体同样具有良好的适用性。各群体最小等位基因频率均值为0.240~0.267，与国外主流养殖群体相近，黑龙江虹鳟、四川虹鳟和北京虹鳟群体内遗传多样性丰富，多态位点比例为83.6%~84.9%，与国外主流养殖群体相近，而黑龙江金鳟、四川金鳟和北京金鳟，多态位点比例相对较低，在60.2%~76.9%范围内。应用6个中国虹鳟群体和2个美国虹鳟群体数据开展系统发育分析、主成分分析和群体遗传结构STRUCTURE分析，结果显示8个群体可分为3个祖源类群，其中3个金鳟群体为遗传联系较紧密的一个类群，黑龙江虹鳟和北京虹鳟为一个类群，而四川虹鳟与2个美国虹鳟群体为一个类群，部分中国养殖群体中有显著离群个体存在，表明群体遗传背景不均一。本研究表明，高密度SNP芯片在我国虹鳟养殖群体遗传分析中具有广泛的应用前景，能够为种质资源评估、本土化良种培育、制种和引种工作提供基因组水平的参考信息。 Abstract:The present study aimed to perform a genome-wide genetic evaluation of representative aquaculture populations of rainbow trout () in China. A 57K single nucleotide polymorphism (SNP) array that was designed by the National Center for Cool and Cold Water Aquaculture (NCCCWA) was used to genotype 48 individuals from six aquaculture populations, which included the Heilongjiang rainbow trout (1HLJ), Heilongjiang golden trout (2gHLJ), Sichuan rainbow trout (3SC), Sichuan golden trout (4gSC), Beijing rainbow trout (5BJ), and Beijing golden trout (6gBJ). A total of 50,201 SNPs were successfully genotyped using a >97% call rate threshold, and the overall rate of polymorphism was 97.7%, which indicated that the SNP array was suitable for analyzing the Chinese rainbow trout populations, even though it was designed using USA and Norwegian populations. The average minor allele frequency of each population was between 0.240 and 0.267, which is similar to that reported for other major aquaculture populations. Genetic diversity was high in the 1HLJ, 3SC, and 5BJ populations, with rates of polymorphism ranging from 83.6% to 84.9%, which is similar to reported for other major aquaculture populations. However, genetic diversity was relatively low in the 2gHLJ, 4gSC, and 6gBJ populations, with rates of polymorphism ranging from 60.2% to 76.9%. Phylogenetic, principal components, and STRUCTURE analyses, which were performed using genotype data from the six Chinese populations and two USA populations, indicated that the eight populations could be clustered into three groups. The three golden trout groups and the 1HLJ and 5BJ populations formed respective clusters, whereas the 3SC population was placed in a third cluster with the two USA populations. Outliers were identified for the 3SC, 4gSC, and 6gBJ populations, which suggested that the populations possessed inhomogeneous genetic backgrounds. Together, these results demonstrate the promising potential of high-density SNP arrays for the genetic analysis of Chinese rainbow trout populations and that such analyses could provide genomic data for plasm resource evaluation, localized strain breeding, high-quality fry production, and reasonable foreign strain introduction. 参考文献 相似文献 引证文献

Study on phylogenetic relationships of Euphausiacea based on mitochondrial COI gene sequences

PDF HTML阅读 XML下载 导出引用 引用提醒 基于线粒体COI基因序列的磷虾目分子系统进化 DOI: 作者: 作者单位: 中国水产科学研究院东海水产研究所, 农业农村部远洋与极地渔业创新重点实验室, 农业农村部东海渔业资源开发利用重点实验室, 上海 200090 作者简介: 谌微(1989-),助理研究员,研究方向为水产动物遗传育种.E-mail:328160296@qq.com 通讯作者: 中图分类号: S931 基金项目: 科技部科技基础性工作专项（2013FY110700）；中央级公益性科研院所基本科研业务费（2014A11）；国家科技支撑计划课题（2013BAD13B03）；国家自然科学基金青年科学基金项目（41406190）. Study on phylogenetic relationships of Euphausiacea based on mitochondrial COI gene sequences Author: Affiliation: Key and Open Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture and Rural Affairs;East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:为探讨磷虾目物种的系统进化关系，以磷虾目的50种磷虾为研究对象，分析其线粒体COI基因序列的分子变异，构建系统发育树，初步探讨了磷虾种属间亲缘关系。所分析COI基因可比序列长度为519 bp，共包含258个变异位点，全部为碱基替换，无插入/缺失位点。四种碱基含量分别为A 27.58%、T 35.47%、G 18.88%、C 18.07%，碱基A+T含量（63.05%）显著高于G+C含量（36.95%），表现出明显的T偏倚特点。50种磷虾的种间遗传距离为0.065~0.306，其平均值为0.186；而种内遗传距离为0~0.071，其平均值为0.017，平均种间距离约为种内距离的11倍。根据物种鉴定最小种间遗传距离0.020的观点，COI基因序列间的差异能够很好地区分各磷虾种。基于COI基因分别构建了4种系统进化树：邻接树（neighbor-joining，NJ）、极大似然树（maximum likelihood，ML）、最大简约树（maximum parsimony，MP）及UPGMA（unweighted pair-group method with arithmetic means）树。它们拓扑结构基本一致，均可分为三大支系：假磷虾属处于系统树的基部，是磷虾目中最早分化出来的一支；而包含磷虾种类最多的磷虾属则最后分化出来，表明其为磷虾类中相对较新的一个属。本研究较全面地阐述了磷虾目的系统进化关系，结果表明磷虾目的线粒体COI序列变异可以用来研究磷虾属、种的分类单元及其系统进化问题，具有较好的理论和应用价值。 Abstract:In order to study the phylogenetic relationship of Euphausiacea, 50 Euphausiacea species were selected and used for the construction of a phylogenetic tree. The mitochondrial COI gene was amplified using PCR techniques. The results showed that a 519 bp COI gene fragment was obtained and sequenced, with 258 mutation sites, all of which were base substitutions, and no insertion/deletion sites. The contents of A, T, G, and C were 27.58%, 35.47%, 18.88%, and 18.07% respectively. Additionally, the content of A+T (63.05%) was significantly higher than that of G+C (36.95%). The genetic distance within species ranged from 0 to 0.071 and the average value was 0.017. The genetic distance among species ranged from 0.065 to 0.306, and the average value was 0.186, which was about 11 times that of the former. With regards to the minimum interspecific genetic distance of 0.020, the difference between the COI gene sequences could distinguish the Euphausiacea species efficiently. Four phylogenetic trees were constructed:neighbor-joining tree (NJ), maximum likelihood tree (ML), maximum parsimony tree (MP), and UPGMA tree. Their topological structures were basically the same, i.e. they can be divided into three branches. Pseudeuphausia was on the bottom of the phylogenetic tree, which was one of the earlier species of Euphausiacea. The Euphausia, which contained the most species, finally was branched out, indicating that it was a relatively newer genus of Euphausiacea. The phylogenetic relationships among 11 Euphausiacea genera have been comprehensively discussed in this paper. The results showed that the phylogenetic tree constructed based on the mitochondrial COI gene could be used to study the phylogenetic relationships between the Euphausiacea species and was of notable theoretical and practical value. 参考文献 相似文献 引证文献

Rhizoclonium grande Bioethanol in Biofuel Production by Transesterification of Jatropha curcas Oil

There is a need for an alternative sustainable fuel based on renewable sources and efficient carbon dioxide scrubbers. Alkyl esters prepared by transesterification of vegetable oils from various plants have been evaluated. In this work the potential of sustainable macroalgae bioethanol in biofuel production by transesterification of Jatropha curcas oil was assessed. Rhizoclonium grande macroalgae was collected from Shimoni shores in Kwale, Shelly beach in Mombasa, Jamvi la Wageni in Mtongwe Likoni, English point near Kenya Marine and Fisheries Research Institute (KMFRI) Mombasa, dried and processed to obtain 5.36% ± 0.355% v/w bioethanol using Aspergilus niger for hydrolysis and Saccharomyces cerevisiae for fermentation. J. curcas seeds were collected from contracted farmers of Energy Africa in Shimba hills, Coast region. Oil from the seeds was machine cold pressed and solvent extracted using n-hexane giving 44% - 53%v/w yield. The physicochemical properties of the J. curcas oil were investigated. Transesterification of J. curcas oil was carried out using bioethanol from the algae with 63% - 70% FAEE yield. Characterisation of the bioethanol and ethyl esters was done using GC-MS. Physicochemical and fuel properties of the biofuel were investigated at Technical University of Mombasa (TUM), Jomo Kenyatta University of Agriculture and Technology (JKUAT), Government Chemist and Kenya Pipeline Laboratories in Mombasa. There was significant difference in physicochemical and fuel properties observed in density, calorific value, kinematic viscosity, pour point and cloud point between the Jatropha oil and Jatropha fatty acid ethyl esters (JAT FAEE) samples. No significant difference observed in the physicochemical and fuel properties between the JAT FAEE and standard biodiesel samples. This was according to statistical analysis of data done using STATA/SE 13.0 and Xlstat at 95% confidence level (P < 0.05) two-tailed. From the findings bioethanol from R. grande biomass used in transesterification has a potential to improve the sustainability, physicochemical and fuel properties of biofuel from J. curcas a non-food crop. The effect of the use of bioethanol and its byproduct on shelf life of the biofuel can further be investigated.

Assessment of economic feasibility of pen aquaculture technology in floodplain wetlands (beels) of Assam, India

Floodplain wetlands (beels) constitute one of the most important fishery resources amenable for culture-based fisheries development in Assam, both in terms of large resource size (100,815 ha) and high fish production potential (1000-1500 kg ha-1 yr-1). Pen aquaculture technology standardised by ICAR-Central Inland Fisheries Research Institute (ICAR-CIFRI), Barrackpore, India for in situ rearing of carp seed for stocking in beels was demonstrated in 21 beels located in 9 districts of Assam in collaboration with the Assam Fisheries Development Corporation Limited (AFDC Ltd.) and Bodoland Territorial Council (BTC) for pilot-scale validation of economic feasibility of the technology. Large pen enclosures (2400-4990 m2) were erected in the beels for the validation; which were grouped into four size groups viz., P1 (2400-2499 m2), P2 (2500-3499 m2), P3 (3500-4499 m2) and P4 (4500-4999 m2 area) to determine the most economic size of pens for the beels of Assam. Cost of bamboo (main construction material) ranged from 30.47 (P4) to 48.74% (P3) of the capital cost. Labour cost for pen construction was found to be the major contributor of capital cost ranging from 33.73 (P3) to 52.22% (P4), followed by the cost of LDPE netting (10.56-13.74%) and coir rope (3.28-5.83%). Major share of recurring cost was spent on fish seed (54.93-61.04%), followed by feed (17.01-22.63%) and towards labour for culture operations (8.48-14.51%). The total cost per crop was the highest in P3 (`87,211/-) followed by that in P4 (`52,606/-) and P2 (`50,933/-), whereas it was the lowest in P1 (`49,511/-). The percentage expenditure of recurring costs per crop to total cost was 72.48-82.25% and a much smaller percentage (17.74-27.51%) of total cost was contributed by the cost on fixed expenditure. The average quantity of fish seed produced in the pens in P1, P2, P3 and P4 groups was 885.94, 875.08, 1,356.73 and 1,104.62 kg, respectively. The P1 group was found to be the most economically viable pen size yielding the highest benefit-cost ratio (3.04), followed by pen sizes P4 (2.78), P2 (2.50) and P3 (2.28). The study showed that pen aquaculture technology using pen sizes in the range of 2400-5000 m2 were economically viable for in situ carp seed rearing in the beels of Assam, with highest profitability in the pen size range of 2400-2499 m2.

Reply to Comment on “Identification of Major Sources of Atmospheric NH3 In An Urban Environment In Northern China During Wintertime”

ADVERTISEMENT RETURN TO ISSUEPREVCorrespondence/Rebut...Correspondence/RebuttalNEXTReply to Comment on “Identification of Major Sources of Atmospheric NH3 In An Urban Environment In Northern China During Wintertime”Qingjing Hu† and Xiaohong Yao*‡View Author Information† Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China‡ Key Lab of Marine Environmental Science and Ecology (MoE), Ocean University of China, Qingdao 266100, China*Phone: 86-532-66782565; fax: 86-532-66782810; e-mail: [email protected]Cite this: Environ. Sci. Technol. 2018, 52, 1, 364–365Publication Date (Web):December 14, 2017Publication History Published online14 December 2017Published inissue 2 January 2018https://doi.org/10.1021/acs.est.7b05839Copyright © 2017 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views661Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (239 KB) Get e-AlertsSUBJECTS:Atmospheric chemistry,Computer simulations,Deposition,Nanogenerators,Particulate matter Get e-Alerts

New records of Solenocera barunajaya Crosnier, 1994 and Solenocera rathbuni Ramadan, 1938 (Crustacea: Decapoda: Penaeoidea) from the southwest coast of India.

The genus SolenoceraLucas, 1849consist of 43 species, most with commercial importance and occurring in offshore, deeper waters (Pérez Farfante & Kensley 1997; De Grave & Fransen 2011). Twelve species in the genus Solenocera were reported from India, with a few contributing to the regular fishery (S. crassicornis, S. choprai, and S. hextii), and others seldom captured (S.alfonso, S.alticarinata, S.annectens, S.halli, S.koelbeli, S.melantho, S.pectinata, S.pectinulata and S.waltairensis). Members of the Solenoceridae are identified based on the key taxonomic characters of the genitalia and the cephalothorax. Major taxonomic references on solenocerids include Crosnier (1978, 1989), Perez Farfante (1977), and Dall (1999). Specimens are deposited in the Marine Biodiversity Museum at the Central Marine Fisheries Research Institute, Kerala (CMFRI).

Technicalities to be considered for culture fisheries development in Indian inland waters: seed and feed policy review

The government and premier fisheries research institutes of India are in pursuit to sensitize the fish farmers and entrepreneurs regarding prospects of highly promising ‘culture-based fisheries (CBFs) technology’ in inland waters. Some crucial aspects, key drivers and existing policy-level lacunae having implications for culture fisheries development in inland waters from fish farmers and fisheries experts perspective have been discussed. Although the technicalities encompassing fish seed and feed are discussed in Indian context, the information may be useful for other developing countries as well. The opinions from fisheries policy, vision documents, meeting proceedings, experts and stakeholders have been compiled to suggest some advanced strategies for hastening culture fisheries development in inland waters and its easier acceptability among stakeholders. Based on recommendations, a centralized fish seed and feed vigilance-cum-certification system is the need of the hour. Stakeholder extracted technicalities and ambiguities surrounding seed and feed aspects can be the basis on which these systems shall function. Periodic upgradation of policies is suggested through feedbacks obtained from stakeholders, especially fish farmers, and shall not be limited to experts only.

Development and characterization of 13 polymorphic microsatellite DNA markers for pink salmon (Oncorhynchus gorbuscha) using next-generation sequencing approach

Journal of Applied IchthyologyVolume 33, Issue 6 p. 1204-1207 SHORT COMMUNICATION Development and characterization of 13 polymorphic microsatellite DNA markers for pink salmon (Oncorhynchus gorbuscha) using next-generation sequencing approach H. Tsukagoshi, Corresponding Author H. Tsukagoshi tsuk@iwate-u.ac.jp orcid.org/0000-0003-2601-805X Sanriku Fisheries Research Center, Iwate University, Kamaishi, Iwate, Japan Correspondence Hideharu Tsukagoshi, Sanriku Fisheries Research Center, Iwate University, Kamaishi, Japan. Email: tsuk@iwate-u.ac.jpSearch for more papers by this authorS. Terui, S. Terui Sanriku Fisheries Research Center, Iwate University, Kamaishi, Iwate, JapanSearch for more papers by this authorS. Sato, S. Sato Hokkaido National Fisheries Research Institute, Japan Fisheries Research and Education Agency, Sapporo, Hokkaido, JapanSearch for more papers by this authorS. Abe, S. Abe Sanriku Fisheries Research Center, Iwate University, Kamaishi, Iwate, JapanSearch for more papers by this author H. Tsukagoshi, Corresponding Author H. Tsukagoshi tsuk@iwate-u.ac.jp orcid.org/0000-0003-2601-805X Sanriku Fisheries Research Center, Iwate University, Kamaishi, Iwate, Japan Correspondence Hideharu Tsukagoshi, Sanriku Fisheries Research Center, Iwate University, Kamaishi, Japan. Email: tsuk@iwate-u.ac.jpSearch for more papers by this authorS. Terui, S. Terui Sanriku Fisheries Research Center, Iwate University, Kamaishi, Iwate, JapanSearch for more papers by this authorS. Sato, S. Sato Hokkaido National Fisheries Research Institute, Japan Fisheries Research and Education Agency, Sapporo, Hokkaido, JapanSearch for more papers by this authorS. Abe, S. Abe Sanriku Fisheries Research Center, Iwate University, Kamaishi, Iwate, JapanSearch for more papers by this author First published: 25 July 2017 https://doi.org/10.1111/jai.13436Citations: 2Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Citing Literature Volume33, Issue6December 2017Pages 1204-1207 RelatedInformation

Hemolymph haemagglutination activity of pearl oysters Pinctada fucata in post-operative care

Aquaculture ResearchVolume 48, Issue 11 p. 5690-5692 SHORT COMMUNICATION Hemolymph haemagglutination activity of pearl oysters Pinctada fucata in post-operative care Natsumi Sano, Corresponding Author Natsumi Sano natsumi5@bio.mie-u.ac.jp orcid.org/0000-0002-8485-792X Graduate School of Bioresources, Mie University, Tsu, Mie, Japan Correspondence Natsumi Sano, Graduate School of Bioresources, Mie University, Tsu, Mie, Japan. Email: natsumi5@bio.mie-u.ac.jpSearch for more papers by this authorTakashi Atsumi, Takashi Atsumi Mie Prefecture Fisheries Research Institute, Shima, Mie, JapanSearch for more papers by this authorShinji Tanaka, Shinji Tanaka Mie Prefecture Fisheries Research Institute, Shima, Mie, JapanSearch for more papers by this authorAkira Komaru, Akira Komaru Graduate School of Bioresources, Mie University, Tsu, Mie, JapanSearch for more papers by this author Natsumi Sano, Corresponding Author Natsumi Sano natsumi5@bio.mie-u.ac.jp orcid.org/0000-0002-8485-792X Graduate School of Bioresources, Mie University, Tsu, Mie, Japan Correspondence Natsumi Sano, Graduate School of Bioresources, Mie University, Tsu, Mie, Japan. Email: natsumi5@bio.mie-u.ac.jpSearch for more papers by this authorTakashi Atsumi, Takashi Atsumi Mie Prefecture Fisheries Research Institute, Shima, Mie, JapanSearch for more papers by this authorShinji Tanaka, Shinji Tanaka Mie Prefecture Fisheries Research Institute, Shima, Mie, JapanSearch for more papers by this authorAkira Komaru, Akira Komaru Graduate School of Bioresources, Mie University, Tsu, Mie, JapanSearch for more papers by this author First published: 02 May 2017 https://doi.org/10.1111/are.13370Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume48, Issue11November 2017Pages 5690-5692 RelatedInformation

Fish habitat and fisheries of Brahmaputra River in Assam, India

River Brahmaputra is a trans-boundary major river flowing through the northeastern state of Assam, India and is the lifeline of its natural fisheries. In the present article, we discuss eco-hydrobiology (including sediment quality and energy flow) of River Brahmaputra and its important tributaries in the state of Assam, India based on extensive synoptic studies conducted from April 1996 to March 1998, duly collated with past and present studies. Salient aspects of fisheries of the river system in the state (ichthyofauna, biogeography, conservation status, migratory and exotic fish species, trends in fish yield and species composition, fishing crafts and gear) are discussed in light of past and present studies conducted by the Indian Council of Agricultural Research-Central Indian Fisheries Research Institute and others. Fisheries of floodplain wetlands (beels) located in the Brahmaputra valley in the state and their interaction with the river system are also discussed.