Analysis of the bacterial community composition of the epiphytes on diseased Gracilaria lemaneiformis using PCR-DGGE fingerprinting technology

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 PCR-DGGE指纹图谱技术分析发病龙须菜附生菌菌群组成 DOI: 作者: 作者单位: 1. 汕头大学 理学院 生物系, 广东省海洋生物技术重点实验室, 广东 汕头 515063;2. 汕头大学 理学院, 汕头大学-马尔凯理工大学藻类联合研究中心, 广东 汕头 515063;3. 福建省水产研究所, 福建省海洋生物增养殖与高值化利用重点实验室, 福建 厦门 361013 作者简介: 裴鹏兵(1990-),男,硕士研究生,研究方向为水生环境保护与修复.E-mail:peipengbing1990@126.com 通讯作者: 中图分类号: S917;Q935 基金项目: 广东省海洋渔业科技与产业发展专项项目（A201405B06）；福建省海洋生物增养殖与高值化利用重点实验室开放课题基金项目（2016fjscq02）. Analysis of the bacterial community composition of the epiphytes on diseased Gracilaria lemaneiformis using PCR-DGGE fingerprinting technology Author: Affiliation: 1. Guangdong Provincial Key Laboratory of Marine Biotechnology, Department of Biology, College of Science, Shantou University, Shantou 515063, China;2. Inauguration of STU-UNIVPM Joint Algal Research Center, College of Science, Shantou University, Shantou 515063, China;3. Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:本研究以07-2品系龙须菜（）为实验材料，提取自然条件下代表不同病变程度的健康、病间、白化藻体的附生菌DNA，经PCR扩增16S rDNA基因的V3可变区并进行变性梯度凝胶电泳（DGGE），分离V3片段，再对目的条带进行克隆鉴定。实验结果经数据化后计算龙须菜附生菌数目并比较附生菌菌群多样性，通过非度量多维尺度分析（nMDS）及多元梯度分析（RDA）来比较附生菌菌群组成相似性及优势菌群。实验结果表明，随着龙须菜病变程度的加深，附生菌菌落数及菌群多样性逐渐增加（<0.05）。nMDS分析结果表明，健康藻体与白化藻体在附生菌菌群组成上存在差异，病间藻体在附生菌菌群组成上与健康藻体更为接近。RDA分析结果表明，龙须菜健康藻体附生菌可能的优势菌群是海洋单胞菌属（）、红杆菌科（Rhodobacteraceae）、假交替单胞菌属（、红杆菌科、玫瑰变色菌属（）。从上述结果可以看出，龙须菜健康藻体与发病藻体在附生菌菌群组成及优势菌上存在较大差异，而介于两者之间的藻体在菌群组成上更偏向于健康藻体。 Abstract:, one of the important sources of agar, is the third most widely cultivated seaweed in China, after . In recent years, there has been a large-scale decrease in the cultivation of , resulting in huge economic losses for the seaweed cultivation industry. Epiphytes grow as an integral part on the surfaces of large algae, and the variations in the seaweed-associated microbial community compositions are typically associated with disease occurrence. In this study, the 07-2 strain was used as experimental material. DNA of epiphytes from the healthy, adjacent, and bleached fronds was extracted, and the V3 variables amplified from 16S rDNA genes were carried out by denaturing gradient gel electrophoresis (DGGE). Finally, the 23 objective bands were cloned and identified. The number and diversity index of the bacterial community of the epiphytes from the three fronds were calculated after digitization of the experimental results. The similarity among the dominant species in the bacterial communities were compared by non-metric multidimensional scaling (nMDS) and redundancy analysis (RDA). The results show that the colony forming units and bacterial community diversity increased gradually with the aggravation in the degree of lesion in <0.05). The nMDS results indicate that the bacterial community composition of the epiphytes varies between the healthy and bleached fronds, while the composition of adjacent fronds is closer compared with that between healthy fronds. The RDA results reveal that the potential dominant bacteria of the epiphytes of the healthy and adjacent fronds are Pseudoalteromonas, respectively. However, the potential dominant bacteria of the epiphytes of the bleached fronds were not identified. The dominant bacteria of the epiphytes of the bleached attached fronds were . It can be seen from the above results that there is a significant difference between the healthy fronds and the diseased fronds of in terms of the bacterial community composition and dominant bacteria of the epiphytes, while the epiphytic bacterial community composition of the adjacent fronds was more similar than that of the healthy fronds. 参考文献 相似文献 引证文献