Effects of salinity on osmotic pressure and nonspecific immune enzymes in juvenile Eniocheir sinensis

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 盐度对中华绒螯蟹仔蟹渗透压和非特异性免疫酶的影响 DOI: 作者: 作者单位: 1. 中国水产科学研究院东海水产研究所, 上海 200090;2. 上海海洋大学水产与生命学院, 上海 201306 作者简介: 徐静静(1992-),女,硕士研究生,从事水生动物生理生态学研究.E-mail:xujingdhs@163.com 通讯作者: 中图分类号: S93 基金项目: 农业农村部财政专项（CJDC-2017-23）；中央级公益性科研院所基本科研业务费项目（2016M05）；上海市中华绒螯蟹产业技术体系项目（沪农科产字2017-4号）. Effects of salinity on osmotic pressure and nonspecific immune enzymes in juvenile Eniocheir sinensis Author: Affiliation: 1. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Shanghai 200090, China;2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:设置盐度梯度0、4、8、12、16、20和24，测定不同盐度下中华绒螯蟹（-ATPase活性、氧合血蓝蛋白含量以及超氧化物歧化酶（SOD）、碱性磷酸酶（AKP）、酚氧化酶（PO）活性，研究盐度对仔蟹成活率、渗透压调节和非特异性免疫酶活性的影响，探讨仔蟹对盐度的适应性。结果表明，盐度8实验组中华绒螯蟹仔蟹成活率和氧合血蓝蛋白含量最高，分别为（90.00±1.00）%和（0.253±0.023） mg/mL，显著高于其他实验组（+浓度，Na+-K+-ATPase活性和非特异性免疫酶活性在盐度0~8时呈下降趋势，而在盐度8~12时逐渐上升，当盐度大于12时趋于稳定；这些指标值在盐度8实验组降到最低，Na+、Cl-和K+浓度分别为（40.897±1.700） mmol/mL、（8.340±2.130） mmol/mL和（1.842±0.158） mmol/mL，Na+-K+-ATPase酶活性为（3.153±0.735） U/mg，非特异性免疫酶SOD、AKP和PO活性分别为（129.026±3.496） U/mg、（1.326±0.173） U/mg和（16.366±0.065） ng/mL。综合各项实验指标表明，不同盐度对中华绒螯蟹仔蟹渗透压和非特异性免疫酶活性具有一定影响，中华绒螯蟹仔蟹在盐度4~8水体中有较好的生理适应性，淡水和高盐度水体会降低其渗透压调节和免疫防御功能，这为长江口仔蟹资源变动评估和繁育场保护提供了参考。 Abstract:Salinity is an important ecological factor in the environment. It not only affects growth but also survival rate, osmotic pressure, and non-specific enzymes in juveniles of E. sinensis in the Yangtze Estuary, the effects of salinity on survival rate, osmotic pressure, and non-specific enzymes of juvenile were studied. Juveniles were added to circular glass fiber cylinders with different salinity levels (0, 4, 8, 12, 16, 20, and 24) and maintained under the following conditions:average water temperature 23-24; pH 7.2℃ -7.8; and average DO>5 mg/L. The survival rate, tissue homogenate supernatant Na+, Cl-, and K+ concentrations, Na+-K+-ATPase activity, oxyhemoglobin content, and the activities of total superoxidase (SOD), alkaline phosphatase (AKP), and phenoloxidase (PO) were analyzed after 3 days of the experiment. The results showed that the salinity 8 experimental group had the highest survival rate and hemocyanin content, which were 89% and (0.253±0.023) mg/mL, respectively, and were significantly higher than those of other experimental groups (<0.05) but were minimized in the freshwater and salinity 24 groups. Na+, Cl-, and K+ concentrations, Na+-K+-ATPase activity, and non-specific immune enzyme activity were divided into three stages. Firstly, these indicators decreased significantly with increasing salinity (0-8), gradually increased with higher salinity (8-12), and finally stabilized at the highest salinity (12-24). It had its lowest index values in the salinity 8 experimental group, the concentrations of Na+, Cl- and K+ were (40.897±1.700) mmol/mL, (8.340±2.130) mmol/mL, and (1.842±0.158) mmol/mL, and the activity of Na+-K+-ATPase was (3.153±0.735) U/mg, and the activities of SOD, AKP, and PO were (129.026±3.496) U/mg, (1.326±0.173) U/mg, and (16.366±0.065) ng/mL, respectively. Together, the results revealed that the juveniles were highly adaptable in low salinity (4-8), whereas fresh water and high salinity water weakened its osmotic regulation and immune defense function. The goals of this study were to reveal the physiological characteristics during feed-bait migration and provide a scientific basis for resource conservation and environmental assessment of juvenile . 参考文献 相似文献 引证文献