Microsatellite genetic analysis of gynogenetic grass carp group and two common grass carp groups

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 雌核发育草鱼群体及两个普通草鱼群体的微卫星遗传分析 DOI: 作者: 作者单位: 山东省淡水渔业研究院, 山东省淡水水产遗传育种重点实验室, 山东 济南 250017 作者简介: 朱树人(1986-),男,博士,研究方向为水产动物遗传育种.E-mail:zhshr229@163.com 通讯作者: 中图分类号: S917 基金项目: 国家自然科学基金项目（31440090）；国家大宗淡水鱼产业技术体系济南综合试验站项目（CARS-45-43）；山东省农业良种工程项目（2014lz042）；山东省现代农业产业技术体系鱼类创新团队项目（SDAIT-14-011-01）；山东省重点研发计划项目（2018GNC110034）. Microsatellite genetic analysis of gynogenetic grass carp group and two common grass carp groups Author: Affiliation: Shandong Provincial Key Laboratory of Freshwater Genetics and Breeding, Shandong Freshwater Fisheries Research Institute, Jinan 250017, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:以草鱼（）养殖群体（YZ）为母本，以紫外线灭活的鲤鱼精子激活草鱼卵子，冷休克抑制第二极体排出的方法诱导获得异精雌核发育草鱼群体（CH）。利用12对微卫星引物对YZ群体、YS （扬州广陵长江系家鱼原种场引进草鱼群体）群体、CH群体进行PCR扩增并分析，共检测出194个等位基因，其中75.8个有效等位基因。YZ群体、YS群体、CH群体的平均等位基因数依次为13.0、12.6、4.7；平均有效等位基因数依次是7.7、6.6、2.3；平均期望杂合度依次为0.87、0.82、0.56；平均多态信息含量依次为0.84、0.79、0.49。从每个个体在微卫星位点的纯合率看，YZ群体中个体的纯合度在0.00~0.33，YS群体中个体的纯合度在0.00~0.42，CH群体中个体的纯合度在0.42~0.92。这表明与YZ群体和YS群体相比，CH群体的遗传多样性显著下降，并且在每个位点的纯合率CH群体均高于普通草鱼群体，表明人工诱导减数雌核发育可加速草鱼大多数基因位点的纯合，是快速建立高纯品系的有效手段。同时，本研究筛选并利用微卫星位点组合建立了雌核发育草鱼子代不同家系及其母本亲缘关系的简易、高效鉴别技术，旨在为雌核发育草鱼标记辅助育种打下基础。 Abstract:We induced the gynogenetic offspring of the breeding grass carp (YZ) using UV-inactivated grass carp sperm and cold shock method to inhibit extrusion of the second polar body. Twelve pairs of microsatellite primers were used for amplification and genetic analysis of the YZ population and control population (YS). A total of 194 alleles were detected in the YZ and YS populations, of which 75.8 were effective alleles. The average number of alleles in the YZ, YS and CH populations was 13.0, 12.6, and 4.7, respectively; the average number of effective alleles was 7.7, 6.6, and 2.3, respectively; the average expected heterozygosities were 0.87, 0.82, and 0.56, respectively; and the average polymorphism information content was 0.84, 0.79, and 0.49, respectively. Homozygosity was analyzed for the microsatellite loci:the homozygosity of individuals in the YZ population was 0.00-0.33, the homozygosity of individuals in the YS population was 0.00-0.42, and the homozygosity of individuals in the CH population was 0.42-0.92. The results showed that the genetic diversity of the CH population was significantly lower than that of the YZ population and the YS population, and the homozygosity of the CH population at each locus was higher than that of the common grass carp groups. This suggests that artificially induced meiotic gynogenesis can accelerate the homozygosity of most grass carp gene loci and is an effective means of rapidly establishing a high purity strain. At the same time, this study screened and used microsatellite loci combinations to establish a simple and efficient identification technique for the genetic relationship among different families and their female parent, laying a foundation for the marker-assisted breeding of gynogestidium. 参考文献 相似文献 引证文献